Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,429,964	0	C	A	33.3%	104.5 / 15.4	42	intergenic (‑88/+53)	uspF/ompN	universal stress protein UspF/porin OmpN
Reads supporting (aligned to +/- strand):  ref base C (23/5);  new base A (0/14);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 2.20e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TACGCTAAACCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATC  >  NZ_CP009273/1429716‑1430193                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                       tACGCTAAACCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATc                                                                                                                                                                                                                                        >  2:251321/1‑249 (MQ=255) tACGCTAAACCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATc                                                                                                                                                                                                                                        >  2:206356/1‑249 (MQ=255) tACGCTAAACCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCa                                                                                                                                                                                                                                       <  2:364573/250‑1 (MQ=255) tACGCTAAACCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCa                                                                                                                                                                                                                                       <  1:42152/250‑1 (MQ=255)   cGCTAAACCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCaaa                                                                                                                                                                                                                                     <  1:369168/250‑1 (MQ=255)        aaCCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAaggagg                                                                                                                                                                                                                                <  2:372797/250‑1 (MQ=255)            cAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGg‑aaga                                                                                                                                                                                                                           <  2:21376/250‑1 (MQ=255)                         tagGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGaaa                                                                                                                                                                                                                >  2:304702/1‑248 (MQ=255)                                gTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGc                                                                                                                                                                                                        >  1:586755/1‑249 (MQ=255)                                 tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCa                                                                                                                                                                                                      >  2:610449/1‑250 (MQ=255)                                                    aaataccccTTTGAATCATAATTCTTTCCCTCTCCAACAACGGGCCAAACCCCGTGTTAATTTAATT‑TAAAACGGAAAATATCGATAGGAAAAAGATTTTTTCTTTTAAAAAACCCTCTTTTTTTATTACCCGAAAAAAGTGAAACGCAAAATGCCTTTTTTTGTGA‑AATGGGGGATTTCATTTTTTTATTTAAGAAAAAGGGGGA‑AAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGAt                                                                                                                                                                                   <  2:99500/243‑1 (MQ=255)                                                      aTGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGAtt                                                                                                                                                                                  >  2:362344/1‑249 (MQ=255)                                                         aaCCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAg                                                                                                                                                                               >  2:565972/1‑249 (MQ=255)                                                            ctctGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACt                                                                                                                                                                           >  1:527449/1‑250 (MQ=255)                                                             tctGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACt                                                                                                                                                                           >  1:50309/1‑249 (MQ=255)                                                                gCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGAt                                                                                                                                                                        >  1:621895/1‑249 (MQ=255)                                                                                                                   aaTTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCg                                                                                                                      >  1:117303/1‑248 (MQ=255)                                                                                                                    aTTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCg                                                                                                                      >  1:258099/1‑247 (MQ=255)                                                                                                                     ttCTAAATCGA‑AAATATGAAAAGAGACAAAAATCTTTCTTTACAAAAACCCTCTTTTTTTAGTACCCGAATAAAGTTTAACGCAAGATGACTTTTTTTGTTA‑AAGGACGGAGTTCATATTTTTAATTAAGAAAAAAGGAGA‑AAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGa                                                                                                                  <  2:273602/250‑1 (MQ=255)                                                                                                                        tGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGc                                                                                                               >  1:217968/1‑250 (MQ=255)                                                                                                                                 aaaTATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCt                                                                                                       >  2:528649/1‑250 (MQ=255)                                                                                                                                        gATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATcc                                                                                                 >  2:249313/1‑249 (MQ=255)                                                                                                                                               aaaaaaaTCGTTCTTTAAAAAAACCCTCCTTTTTTAGTACCCGAAAAAAGTGAAACGCCAGATAACTTTTTTTGTGA‑AATGACGGAGTTCATATTTTTAATTTAAAAAAAAGGAGA‑AAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGtt                                                                                         <  1:212390/245‑1 (MQ=255)                                                                                                                                                             gTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCaa                                                                           >  1:464441/1‑250 (MQ=255)                                                                                                                                                                 aTAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACg                                                                         >  2:475529/1‑248 (MQ=255)                                                                                                                                                                        ctctttttttAGTACCCGAAAAAAGTGAAACGCAAGATGACTTTTTTTGTAA‑AAAGAAGGAGTCCACATTTTAAATTAAAAAAAAAGGAGA‑AAAAAAAAAAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGAc                                                                <  2:166811/244‑1 (MQ=255)                                                                                                                                                                             tttttAGTATCAGAAAAAGGTGTACCGCAAGATGACATTTTGTGTGA‑AAAAAGGGAATCCAAATTTTAAATAAAGA‑AAAAGGAGGAAAAAAAAAGAAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATg                                                             <  1:379571/247‑1 (MQ=255)                                                                                                                                                                             gTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtt                                                           >  2:579490/1‑250 (MQ=255)                                                                                                                                                                                         gCATAAAGTGTAACGCCAGATTACACTTTTTGTGT‑AATGTCGGAGATCACATTTTTAATTTAGATCAAAGGTTG‑AAGATTTAGCAGATAAAGTCCGCCATAACATCGGTAAGGAGGATTTAGAACTGTTAAACCAGGCATAAATCGACAATATCATCGGTAGAGATGCCATTGGCAGCTTAGATTCTATCATCTTCATCAAGCGGATTGCTTTTATAGTCATCGTAGGTGGACATGGTTTTATTGaatt                                                >  2:166419/1‑247 (MQ=255)                                                                                                                                                                                                gtgtACCGCAAGAGGATATTTTTTGTGA‑AATGACGGAGTTCAAATTTATAATTAAGAAAAAAGGAGA‑AAGAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTc                                        <  1:278947/250‑1 (MQ=255)                                                                                                                                                                                                   tAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTcgcg                                     >  2:103979/1‑250 (MQ=255)                                                                                                                                                                                                   tAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTcgcg                                     >  2:532521/1‑250 (MQ=255)                                                                                                                                                                                                    aacgataGATGACTTTTTTTGTGA‑AAGGAGGGTGTAAACTTTTTAAATGAAGAAAAAAGAAAG‑AAAAATAAAAAAAAAAGGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCgcgc                                    <  1:208223/244‑1 (MQ=255)                                                                                                                                                                                                    aaCGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCgcgc                                    >  2:145784/1‑250 (MQ=255)                                                                                                                                                                                                         caagaagactcttttGGGTAAATGAGGGATTTAAATTTTTTATTTTAAAAAAAAGGAGA‑AAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAat                                <  1:509298/239‑1 (MQ=255)                                                                                                                                                                                                         aagatgattttttttGTGT‑AATGACGGAGTTCATTTTTTAAATAAAAACAAAAGGAGA‑AAAAAAAAAAAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAata                               <  1:373764/240‑1 (MQ=255)                                                                                                                                                                                                                   tttttGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTAtt                     >  1:83450/1‑250 (MQ=255)                                                                                                                                                                                                                     ttgggat‑AAAGACGTAATTCATATTTTAAATTAAAAAAAAAGGAAA‑AAAAAAAAAAAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTAttt                    <  2:217968/243‑1 (MQ=255)                                                                                                                                                                                                                       tgtgT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAAc                 >  2:68459/1‑250 (MQ=255)                                                                                                                                                                                                                       tgtgG‑AATGAGGGATTTCCATTTTTTAATTAAGAAAAAAGGAGA‑AAAAATAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTaa                  <  1:159223/249‑1 (MQ=255)                                                                                                                                                                                                                         ttgaaaTGACGGAGTTCACATTTTTATTTTAGAAAAAAGGAGG‑AAGAAAAAGAAAAAAAAGACCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAAcc                <  1:612624/246‑1 (MQ=255)                                                                                                                                                                                                                               tGACGGATTTCAAATTTTAAATTAAAATAAAAGGAGA‑AAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCt          <  1:442510/250‑1 (MQ=255)                                                                                                                                                                                                                                      gTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTatcat   >  2:541718/1‑250 (MQ=255)                                                                                                                                                                                                                                        caatttttttAATTAAGAGAAAGGAAAG‑AAAAAAAAGCAAAAAACCCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTatcatc  <  2:527449/244‑1 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                       TACGCTAAACCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATC  >  NZ_CP009273/1429716‑1430193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑