Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,429,975	0	G	A	32.4%	64.3 / 12.8	37	intergenic (‑99/+42)	uspF/ompN	universal stress protein UspF/porin OmpN
Reads supporting (aligned to +/- strand):  ref base G (23/2);  new base A (0/12);  total (23/14)
Fisher's exact test for biased strand distribution p-value = 4.91e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATC  >  NZ_CP009273/1429727‑1430193                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                           cAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGg‑aaga                                                                                                                                                                                                                           <  2:21376/250‑1 (MQ=255)              tagGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGaaa                                                                                                                                                                                                                >  2:304702/1‑248 (MQ=255)                     gTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGc                                                                                                                                                                                                        >  1:586755/1‑249 (MQ=255)                      tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCa                                                                                                                                                                                                      >  2:610449/1‑250 (MQ=255)                                         aaataccccTTTGAATCATAATTCTTTCCCTCTCCAACAACGGGCCAAACCCCGTGTTAATTTAATT‑TAAAACGGAAAATATCGATAGGAAAAAGATTTTTTCTTTTAAAAAACCCTCTTTTTTTATTACCCGAAAAAAGTGAAACGCAAAATGCCTTTTTTTGTGA‑AATGGGGGATTTCATTTTTTTATTTAAGAAAAAGGGGGA‑AAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGAt                                                                                                                                                                                   <  2:99500/243‑1 (MQ=255)                                           aTGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGAtt                                                                                                                                                                                  >  2:362344/1‑249 (MQ=255)                                              aaCCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAg                                                                                                                                                                               >  2:565972/1‑249 (MQ=255)                                                 ctctGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACt                                                                                                                                                                           >  1:527449/1‑250 (MQ=255)                                                  tctGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACt                                                                                                                                                                           >  1:50309/1‑249 (MQ=255)                                                     gCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGAt                                                                                                                                                                        >  1:621895/1‑249 (MQ=255)                                                                                                        aaTTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCg                                                                                                                      >  1:117303/1‑248 (MQ=255)                                                                                                         aTTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCg                                                                                                                      >  1:258099/1‑247 (MQ=255)                                                                                                          ttCTAAATCGA‑AAATATGAAAAGAGACAAAAATCTTTCTTTACAAAAACCCTCTTTTTTTAGTACCCGAATAAAGTTTAACGCAAGATGACTTTTTTTGTTA‑AAGGACGGAGTTCATATTTTTAATTAAGAAAAAAGGAGA‑AAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGa                                                                                                                  <  2:273602/250‑1 (MQ=255)                                                                                                             tGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGc                                                                                                               >  1:217968/1‑250 (MQ=255)                                                                                                                      aaaTATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCt                                                                                                       >  2:528649/1‑250 (MQ=255)                                                                                                                             gATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATcc                                                                                                 >  2:249313/1‑249 (MQ=255)                                                                                                                                    aaaaaaaTCGTTCTTTAAAAAAACCCTCCTTTTTTAGTACCCGAAAAAAGTGAAACGCCAGATAACTTTTTTTGTGA‑AATGACGGAGTTCATATTTTTAATTTAAAAAAAAGGAGA‑AAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGtt                                                                                         <  1:212390/245‑1 (MQ=255)                                                                                                                                                  gTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCaa                                                                           >  1:464441/1‑250 (MQ=255)                                                                                                                                                      aTAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACg                                                                         >  2:475529/1‑248 (MQ=255)                                                                                                                                                             ctctttttttAGTACCCGAAAAAAGTGAAACGCAAGATGACTTTTTTTGTAA‑AAAGAAGGAGTCCACATTTTAAATTAAAAAAAAAGGAGA‑AAAAAAAAAAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGAc                                                                <  2:166811/244‑1 (MQ=255)                                                                                                                                                                  tttttAGTATCAGAAAAAGGTGTACCGCAAGATGACATTTTGTGTGA‑AAAAAGGGAATCCAAATTTTAAATAAAGA‑AAAAGGAGGAAAAAAAAAGAAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATg                                                             <  1:379571/247‑1 (MQ=255)                                                                                                                                                                  gTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtt                                                           >  2:579490/1‑250 (MQ=255)                                                                                                                                                                              gCATAAAGTGTAACGCCAGATTACACTTTTTGTGT‑AATGTCGGAGATCACATTTTTAATTTAGATCAAAGGTTG‑AAGATTTAGCAGATAAAGTCCGCCATAACATCGGTAAGGAGGATTTAGAACTGTTAAACCAGGCATAAATCGACAATATCATCGGTAGAGATGCCATTGGCAGCTTAGATTCTATCATCTTCATCAAGCGGATTGCTTTTATAGTCATCGTAGGTGGACATGGTTTTATTGaatt                                                >  2:166419/1‑247 (MQ=255)                                                                                                                                                                                     gtgtACCGCAAGAGGATATTTTTTGTGA‑AATGACGGAGTTCAAATTTATAATTAAGAAAAAAGGAGA‑AAGAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTc                                        <  1:278947/250‑1 (MQ=255)                                                                                                                                                                                        tAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTcgcg                                     >  2:103979/1‑250 (MQ=255)                                                                                                                                                                                        tAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTcgcg                                     >  2:532521/1‑250 (MQ=255)                                                                                                                                                                                         aacgataGATGACTTTTTTTGTGA‑AAGGAGGGTGTAAACTTTTTAAATGAAGAAAAAAGAAAG‑AAAAATAAAAAAAAAAGGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCgcgc                                    <  1:208223/244‑1 (MQ=255)                                                                                                                                                                                         aaCGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCgcgc                                    >  2:145784/1‑250 (MQ=255)                                                                                                                                                                                              caagaagactcttttGGGTAAATGAGGGATTTAAATTTTTTATTTTAAAAAAAAGGAGA‑AAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAat                                <  1:509298/239‑1 (MQ=255)                                                                                                                                                                                              aagatgattttttttGTGT‑AATGACGGAGTTCATTTTTTAAATAAAAACAAAAGGAGA‑AAAAAAAAAAAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAata                               <  1:373764/240‑1 (MQ=255)                                                                                                                                                                                                        tttttGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTAtt                     >  1:83450/1‑250 (MQ=255)                                                                                                                                                                                                          ttgggat‑AAAGACGTAATTCATATTTTAAATTAAAAAAAAAGGAAA‑AAAAAAAAAAAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTAttt                    <  2:217968/243‑1 (MQ=255)                                                                                                                                                                                                            tgtgT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAAc                 >  2:68459/1‑250 (MQ=255)                                                                                                                                                                                                            tgtgG‑AATGAGGGATTTCCATTTTTTAATTAAGAAAAAAGGAGA‑AAAAATAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTaa                  <  1:159223/249‑1 (MQ=255)                                                                                                                                                                                                              ttgaaaTGACGGAGTTCACATTTTTATTTTAGAAAAAAGGAGG‑AAGAAAAAGAAAAAAAAGACCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAAcc                <  1:612624/246‑1 (MQ=255)                                                                                                                                                                                                                    tGACGGATTTCAAATTTTAAATTAAAATAAAAGGAGA‑AAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCt          <  1:442510/250‑1 (MQ=255)                                                                                                                                                                                                                           gTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTatcat   >  2:541718/1‑250 (MQ=255)                                                                                                                                                                                                                             caatttttttAATTAAGAGAAAGGAAAG‑AAAAAAAAGCAAAAAACCCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTatcatc  <  2:527449/244‑1 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                           CAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGG‑AAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGG‑AAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATC  >  NZ_CP009273/1429727‑1430193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑