Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,049,4750AC35.2% 107.5 / 15.5 54G75G (GGT→GGG) glnLnitrogen regulation protein NR(II)
Reads supporting (aligned to +/- strand):  ref base A (30/5);  new base C (1/18);  total (31/23)
Fisher's exact test for biased strand distribution p-value = 5.73e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCTTTGCTGAGCAGCTGCGCCGCGCCACGTAAACCGCCAAGCGGATTTTTAATCTCATGTGCCA‑GGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCA  >  NZ_CP009273/4049227‑4049724
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                         
gCTTTGCTGAGCAGCTGCGCCGCGCCACGTAAACCGCCAAGCGGATTTTTAATCTCATGTGCCA‑GGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAAc                                                                                                                                                                                                                                                          <  2:232406/250‑1 (MQ=255)
 cTTTGCTGAGCAGCTGCGCCGCGCCACGTAAACCGCCAAGCGGATTTTTAATCTCATGTGCCA‑GGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTaaaaac                                                                                                                                                                                                                                                         <  1:187765/250‑3 (MQ=255)
     gCTGAGCAGCTGCGCCGCGCCACGTAAACCGCCAAGCGGATTTTTAATCTCATGTGCCA‑GGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGCTATGCGAGCGCCCTTCGATGACCAGCGTCACTTC‑GTTATCGGTAAACCCTTGc                                                                                                                                                                                                                                                     >  2:509480/1‑250 (MQ=255)
       tGAGCAGCTGCGCCGCGCCACGTAAACCGCCAAGCGGATTTTTAATCTCATGTGCCA‑GGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGccc                                                                                                                                                                                                                                                   >  1:175100/1‑250 (MQ=255)
         agcagcTGCGCCGCGCCACGTAAACCGCCAAGCGGATTTTTAATCTCATGTGCCA‑GGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGcccc                                                                                                                                                                                                                                                  <  1:665547/249‑1 (MQ=255)
                  gccgccccACAAAACCCCCCACGCGGATTTTAAATCCCATGGCCCG‑GGCCGCGCACTAAACCCCGGGCAGCACCCTGCGGGGCGGGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGCCCCATCTCCAGCAGAATCATCCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAACCCTTGCCCCGCCTCCAGAc                                                                                                                                                                                                                                        <  2:443167/250‑1 (MQ=255)
                      cccaccgaaacccGCCAGGCGGTTTTTAAATCTAATGGGCCG‑GCCCGCGCACTAAACCCGGGGCAGCAACCTGCGGGGCGTGCGGTAGCTGTTCCTGACTTAAGCGGCGCTGTTTATCCATCGGAGCCATCCCCAGCAGGATCATGCCGCCCGGCATACGCGGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGCCCGGCGTCCCTTC‑TTTATCGGTAAACCCTTGCCCCGCCTCCAGACTTTc                                                                                                                                                                                                                                    <  2:294591/239‑1 (MQ=255)
                            gaaacccGCCAGGCGGTTTTTTAATCTCATG‑GCCAGGGCCGCGCACTAAATCCGGGGCAGCACCCGGGTGGGCGGGCTGTAGCTTTTCCTGATTTAAGCGGCGCTGGTTATCCATCGGACCCATCCCCAGCAGGATCATGCCGTCCGGCATCCGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGCCCAGCTTCCTTTC‑TTTATCGGTAAACCCTTGCCCCGCCTCCAGACTTTCTTGCAt                                                                                                                                                                                                                              <  2:327921/245‑1 (MQ=255)
                              aaaCCGCCAAGCGGATTTTTAATCTCATGTGCCA‑GGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCAt                                                                                                                                                                                                                              >  2:722009/1‑248 (MQ=255)
                                        ttggATTTTTATTCTCGTGTGCCA‑GGCCGCGCACTAAATCACGGGCAGCACCCTGCGGGGGGTGCTGTAGCTGTTCCTGACTTAGGCGGCGCTGGTTATCCATCGGAGCCATCCCCAGCAGGTTCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCATTTC‑GTTATCGTAAAACCCTTCCCCCGCCTCCAGACTTTCTTGCATCAGCTCGatat                                                                                                                                                                                                                   <  2:266091/247‑1 (MQ=255)
                                         cGGATTTTTAATCTCATGTGCCG‑GGCCGCGCCCTAAACCACGGGCAGCACCCTGCTGGGCGTGCTGTGCCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGGGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAACCCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATAttt                                                                                                                                                                                                                 <  1:422875/250‑1 (MQ=255)
                                          ggATTTTTAATCTCATGTGCCA‑GGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGata                                                                                                                                                                                                                    >  1:266091/1‑246 (MQ=255)
                                             tttttAATCTCATGTGCCA‑GGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAAt                                                                                                                                                                                                              >  2:853493/1‑249 (MQ=255)
                                              ttatAACCTCAGGGGCCA‑GGCCGCCTACTAAACCCCGGGCAGCAACCTGCTGGGGGGGCTGTAGCTTTTCCTGACTAAAGCGGCGCTGTTTACCCAGCGGAGCCATCCCCAGCAGGATCATGCCGCCCGGCATACGCGGGGCCGTCACAAAAAGAATAGGCGAGCCCCCGTCGATGCCCAGCGTCCTTTC‑TTTACCGGAAAACCCTTCCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATg                                                                                                                                                                                                             <  2:775056/246‑1 (MQ=255)
                                               tttAACCTCTGGTGCCA‑GCCCGCGCAATAAATCCGGGGCAGCAACCGGTTGGGGGGGCGGTGGCTTTTCCTGACTTAGGGGGCGCTGTTTACCCATGGGACCCATCCCCAGCAGGATCATGCCGCCCGGCATACGCGGGGCCGTCACAAAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGTTAAACCCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATga                                                                                                                                                                                                            <  2:199307/249‑1 (MQ=255)
                                                ttAATCTCATGTGCCA‑GGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATgag                                                                                                                                                                                                           >  1:80778/1‑249 (MQ=255)
                                                 tAATCTCATGTGCCA‑GGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATgag                                                                                                                                                                                                           >  1:122125/1‑248 (MQ=255)
                                                 gagacacATGTGCGA‑GGCCGCGACCAAAGTCACGGACAGCACCCGGCGGGGCGTTCTGTAGCTGTCGCGGACTAAGGGGGCGCTGGATGCCCATCGGACCCATCTCCAGCAGGATCAGGCCGGCCGGCATCCGCGGGGCCGTCACAGAAGGGATAGGCGCGCCCCCGGCGAGGACCGGCGTCGCTGC‑TTTATCGATAAACCCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATgaga                                                                                                                                                                                                          <  2:617002/243‑1 (MQ=255)
                                                     ctcATGTGCCA‑GGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAg                                                                                                                                                                                                     >  1:109282/1‑250 (MQ=255)
                                                                   ccGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCg                                                                                                                                                                                        >  2:833132/1‑250 (MQ=255)
                                                                           tAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATATGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGgt                                                                                                                                                                                >  1:8672/1‑250 (MQ=255)
                                                                            aaaTCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGgtg                                                                                                                                                                               >  2:547315/1‑250 (MQ=255)
                                                                                         catccTGCTGGGCGTGCTGTAGCTGTTCCTGCCTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGCCCAGCGTCACTTC‑GTTATCGGTAAACCCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAAttt                                                                                                                                                                  <  1:330633/247‑1 (MQ=255)
                                                                                          aaCCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGCGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTg                                                                                                                                                                 >  1:803773/1‑250 (MQ=255)
                                                                                             ctgctgGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCgg                                                                                                                                                              >  1:156317/1‑250 (MQ=255)
                                                                                                   ggCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCtt                                                                                                                                                        >  1:315902/1‑250 (MQ=255)
                                                                                                            tgcgtGTTCCTTACTTAGGCGCCGCGGTTTCTCCATCGACGCCATCTCCAGCAGAATCAGGCCGCCGGGCATCCGCTGGGCCGTCACAGAAAGGATATGCGACGGCCCGCCGATGCCCAGCGTCACTTC‑GTTATCGGTAAAACCTGCCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGa                                                                                                                                                 <  2:486123/244‑1 (MQ=255)
                                                                                                             aGCTGTCCCTGACTTAAGCGGCGCTGTTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAACCCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCa                                                                                                                                              <  1:809106/250‑1 (MQ=255)
                                                                                                                tGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAgtt                                                                                                                                           >  1:57920/1‑250 (MQ=255)
                                                                                                                       gACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTgcgc                                                                                                                                    >  1:147058/1‑250 (MQ=255)
                                                                                                                         cTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCgg                                                                                                                                  >  1:734779/1‑250 (MQ=255)
                                                                                                                                             ccgcgtgccctcctcCAGCAGGATCACCCCGCCGGCCATCCGCTGGGCCGCCAAAAAGAGATTAGGCGAGCGCCCGGCGATGACCAGCGTCATTTC‑TTTTTCGGTAAACCTTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATc                                                                                                              <  2:591054/238‑1 (MQ=255)
                                                                                                                                              aTCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCg                                                                                                             >  1:500390/1‑250 (MQ=255)
                                                                                                                                              aTCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCg                                                                                                             >  1:24329/1‑250 (MQ=255)
                                                                                                                                                  gAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTCGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGCAATGGATCGCCAg                                                                                                         >  1:241828/1‑250 (MQ=255)
                                                                                                                                                   gtccATCCCCAGCAGGACCGTCCCGCCGGGCATACGCTGGGCCGCCAAAAAAAGGTGATGCGCCCCCCCTCGGATGCCAGGCGTCATTTC‑GTTATGGAAAAACCCTTCCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAgg                                                                                                        <  2:57920/248‑1 (MQ=255)
                                                                                                                                                     ccATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGtt                                                                                                      >  2:373542/1‑250 (MQ=255)
                                                                                                                                                      cacctcCAGCAGGATCCCGCCGCCCGTCAGCCGCGGGGCCTTCAAAAAAAGGATATGCGAGCCCCCGTCGATGCCCAGCGTCCCTTC‑GTTTGCGGTAAACCCTGCCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTg                                                                                                     <  2:188515/247‑1 (MQ=255)
                                                                                                                                                              gcagGATGATGCCGTCCGACATGCGGTGGGCATTCAAAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCTTCACTTC‑TTTATCGGTAAACCCGTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGAt                                                                                             <  2:358758/250‑1 (MQ=255)
                                                                                                                                                                agagtCATACCGTCCGGCACACGCGGGCCCGCCACGAAAAGTATATGCGGGCCCCGCTAAATGCACAGC‑TCTCTTCAGTTATCGGTAAACCCTTCCACCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTa                                                                                           <  2:796631/246‑1 (MQ=255)
                                                                                                                                                                agGATCATCCCGTCCGGCATCCGCTGGCCCGTCACAGAAAGGATATGCGGGCGCCCTCCGATGCCCAGCGTCACTTC‑TTTATCGGTAAACCCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTa                                                                                           <  1:677958/250‑1 (MQ=255)
                                                                                                                                                                    ccATGCCGTCCAGCATACGGGGGGCCGTAACAGAAAGGATAGGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTAATCGGAAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACaa                                                                                       <  1:260944/249‑1 (MQ=255)
                                                                                                                                                                              cGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGtt                                                                              >  2:272586/1‑249 (MQ=255)
                                                                                                                                                                                  aTACGCTGGGCCGTCACAGAGGGGATAGACGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCa                                                                         <  1:209955/250‑1 (MQ=255)
                                                                                                                                                                                     cGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGc                                                                       >  2:763646/1‑249 (MQ=255)
                                                                                                                                                                                       cggggCCGTAAAGGAAAGGATATGCGAGCCCCCTTCAATACCCAGCGTCCTTTT‑GTTATCGGTAAACCCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAg                                                                    <  1:570544/248‑1 (MQ=255)
                                                                                                                                                                                        tGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGt                                                                   >  2:352792/1‑250 (MQ=255)
                                                                                                                                                                                              gTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCATGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGAGTACCAAACAATTTGCGGGAGCTTTGCGCGAGCAGTTGCTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGa                                                             >  1:23082/1‑250 (MQ=255)
                                                                                                                                                                                                      aaGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGcc                                                       >  1:765929/1‑248 (MQ=255)
                                                                                                                                                                                                                  gcgcCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGc                                         >  2:527127/1‑250 (MQ=255)
                                                                                                                                                                                                                   cgcCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCg                                        >  2:368678/1‑250 (MQ=255)
                                                                                                                                                                                                                            agcaCCAGCGTCGTTTC‑TTTACGGGTAAACCCTTCCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTg                               <  1:582581/247‑1 (MQ=255)
                                                                                                                                                                                                                               aCCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCa                            >  1:180545/1‑250 (MQ=255)
                                                                                                                                                                                                                                  aGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATa                          >  1:619406/1‑249 (MQ=255)
                                                                                                                                                                                                                                              gTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCt              >  2:770438/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       aaaaCCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGtt     >  1:557777/1‑250 (MQ=255)
                                                                                                                                                                                                                                                          aCCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCa  >  2:19534/1‑250 (MQ=255)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                         
GCTTTGCTGAGCAGCTGCGCCGCGCCACGTAAACCGCCAAGCGGATTTTTAATCTCATGTGCCA‑GGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTC‑GTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCA  >  NZ_CP009273/4049227‑4049724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑