Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	341,015	0	G	A	31.1%	143.8 / 33.7	74	intergenic (+313/‑100)	BW25113_RS01680/BW25113_RS01685	protein YahL/protein YahM
Reads supporting (aligned to +/- strand):  ref base G (48/3);  new base A (0/23);  total (48/26)
Fisher's exact test for biased strand distribution p-value = 3.15e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGAAAAAAGCCCACACAGGGGAGAGTGGGCTGAAATGGG  >  NZ_CP009273/340767‑341259                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     aTTTCATCTTTATATCTGTGTGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGGGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAg                                                                                                                                                                                                                                                      <  2:564658/249‑1 (MQ=255) aTTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAg                                                                                                                                                                                                                                                      <  2:636805/249‑1 (MQ=255)  tttCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGaa                                                                                                                                                                                                                                                    <  2:247102/250‑1 (MQ=255)   ttCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGaa                                                                                                                                                                                                                                                    >  1:491337/1‑249 (MQ=255)      atctgtatatctgtatGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAata                                                                                                                                                                                                                                                >  1:420461/1‑250 (MQ=255)         tgtatatctgtatGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGc                                                                                                                                                                                                                                             >  1:336031/1‑250 (MQ=255)           tatatctgtatGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTg                                                                                                                                                                                                                                           >  2:705950/1‑250 (MQ=255)             tatctgtatGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGt                                                                                                                                                                                                                                          >  1:637672/1‑249 (MQ=255)              tgcgaaaccactaCTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGt                                                                                                                                                                                                                                          >  2:646464/13‑248 (MQ=255)                     tGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTCTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATAATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATCTGCTGTAAggtcca                                                                                                                                                                                                                                  >  1:267190/1‑245 (MQ=255)                          aCTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCa                                                                                                                                                                                                                             >  2:587711/1‑249 (MQ=255)                          aCTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCa                                                                                                                                                                                                                             >  2:287109/1‑249 (MQ=255)                           cTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCaa                                                                                                                                                                                                                            >  1:396558/1‑249 (MQ=255)                              gCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCaaaa                                                                                                                                                                                                                          <  1:331268/248‑1 (MQ=255)                                               tCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAg                                                                                                                                                                                                       >  2:642495/1‑250 (MQ=255)                                                  ccgtttttttGTTGTGAGCGGCGACACTTCGCTAAAAAAAGCACCAGTAGTGGTTTCGCAGCCAGGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATAAAAAAAATCATAATCCCATCATGTTTGTGGGGGTTTTTTTCTGTATCTTACGCACCGCACTCAAGCCTGAAAAAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGaa                                                                                                                                                                                                    <  1:457315/244‑1 (MQ=255)                                                     atatTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAagag                                                                                                                                                                                                 >  1:742074/1‑250 (MQ=255)                                                         tttGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAga                                                                                                                                                                                             >  1:726088/1‑250 (MQ=255)                                                             tggtgATCGGCGCCCCTTCGCTAAAAAAACCACCAGTAGTGGTTTCGCACCCATGCGGTTTAAAAAAAATGATCTCATGCAGATGTTTTGTGAATGTTTTGGTTGACATTCATATAAAAAAAATCATAATCCCATCATTTTTGTGTGGGGTCTTTTCTGTATCTTACGCACCGCACTCAAGCCTGAAAAAAAATATGCTGAAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAgacga                                                                                                                                                                                          <  1:160774/247‑1 (MQ=255)                                                                       cGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGCGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATAGGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAtt                                                                                                                                                                               >  2:583926/1‑250 (MQ=255)                                                                         acacTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAttgt                                                                                                                                                                             >  2:257464/1‑250 (MQ=255)                                                                           acTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAttgttg                                                                                                                                                                           >  1:597135/1‑250 (MQ=255)                                                                             ttCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTa                                                                                                                                                                         >  1:513290/1‑250 (MQ=255)                                                                               ggttaaaaaaaGCACCAGTAGTGTTTTCGCACCCATGGGGTGTATAAAAAATGATCCCAGGCAGTTTTTTTGTAATTGTGTTGTTTGACTTTCATATAAAAAAAATCATAATCCCATCATTTTTGGGTGGGTTTTTTTCTGTATTTTACGCACCGCACTAAACCCTGACAAAAAATATGCTGTAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTa                                                                                                                                                                         <  1:116256/243‑1 (MQ=255)                                                                                       aaaGCACCAGTAGTGGTTTCGCACCCATGCGGTTTAAAAAAAATGATCTCATGCAGATGTTTTGTAAATGTTTTGGTTGACATTCATAAAAAAAAAATCATAATCCAATCATGTTTGTGGGGGTTTTTTTCTTTTTCTTCCGAACCGCACTAAAGCCTGACAAAAAATATGCTGTAAGCCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACgggg                                                                                                                                                               <  1:710489/250‑1 (MQ=255)                                                                                                   gtGGTTCCGCAGCCAGGCGGTTAAAAAAAAAGGATCTCATGCAGATTTTTTTTGAATGTTTTGTTTGACATTCATATAAAAAAAATAATAATCCCATCATTTTTGTGTGGGTTCTTTTCTGTATCTTCCCCATCCCCCTCAACCCTAACAAAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTc                                                                                                                                                    <  1:309297/249‑1 (MQ=255)                                                                                                    tGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGa                                                                                                                                                  >  1:324639/1‑250 (MQ=255)                                                                                                        ttgtccgCCAGGGGGTGTAAAAAAAATAATCTCATGAAGATTTTTTGTAATTGTGTTGGTTGACATTAATATAAAAAAAATCAAAATCCCACAATTTTTGTGGGGGTTTTTTTCTGTATCTAACCCATCGCACCAAAGCCTGAAAAAAAATAGGCTGGAAGCCTAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATgg                                                                                                                                               <  2:558248/243‑1 (MQ=255)                                                                                                                 aTGGGGTATAAAAAAAATAATCACATGCAGATTTTTTGAGAGTGTGTGGTTTGACATTAATATAAAAAAAAACAAAATCCCATCATGTTTGGGGGGGTTTTTTTCTGTATCTTACGCACCGCACTAAACCCAGAAAAAAAATATGCTGAAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACt                                                                                                                                     <  2:5090/250‑1 (MQ=255)                                                                                                                 aTGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACt                                                                                                                                     >  1:540188/1‑250 (MQ=255)                                                                                                                            aaaaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAActc                                                                                                                          >  2:457313/1‑250 (MQ=255)                                                                                                                            aaaaaaAGTTCACATGTATATTTTTTGGTATTTTGTTGTTTTATTTTCATAAAAAAAAAATCATACTCCCATATTTTTTGTGTGGTTTTTTTTTTTTTTCTTACCCCCCGCACTAAAGCAAGAAAAAAAAGATGCTATAGGGCTAAAAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAActc                                                                                                                          <  2:188689/250‑1 (MQ=255)                                                                                                                                    aTCTCATGCAGATTTTTTGTGAATTTGTTGTTTGAAATTAATATAAAAAAAATAATAATCCCATCATTTTTGGGGGGGTTCTTTTCTGTTTCTTCCGCATCGCACTCAAGCCTGAAAAAAAATATGCTGAAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGaa                                                                                                                   <  1:248772/249‑1 (MQ=255)                                                                                                                                      ctcATGCTTATGTTTTTTGATTGTTTTGTTTTACATTAATATAAAAAAAATCATATTTCCTTTATTTTTGTGTGGGTTTTTTTCTCTATCTTCCACATCCCCCCCAACCCTAACAAAAATTTTCCTTTAGGTTTCATAACAAAATCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATc                                                                                                                 <  2:732501/249‑1 (MQ=255)                                                                                                                                          ggtattttttttATGAATTTTTTGTTTGACTATAAAAAAAAAAAAATAATATTCCTGTTATTTTTGTGGGGTTTTTTTTTTTTATCTTACGCACCCCACTCAACCCTGAAAAAAAATGTGCTTAAAGGTAAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATc                                                                                                            <  2:411133/242‑1 (MQ=255)                                                                                                                                            cAGATGTTTTGTAAATGTGTTGGTTGACATTCATAAAAAAAAAATCATAATTCCATCATGTTTGGGGGGGTTTTTTTTTGTATCTTCCGCACCCCACTCAAGCCTGAAAAAAAATATGCTGTAGGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATccc                                                                                                          <  1:78183/250‑1 (MQ=255)                                                                                                                                            aatttGTTTTGTGAATGTTTTGGTTGACATTAATATAAAAAAAATCATAATTCCACCATGTTTGTGTGGGGTTTTTTCTGTATCTTCCGCACCCCACTAAAGCCTAACAAAAAATATGCTGTAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATccc                                                                                                          <  1:120439/246‑1 (MQ=255)                                                                                                                                             aGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGCAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATcccc                                                                                                         >  2:516157/1‑250 (MQ=255)                                                                                                                                                  ttttttGATGGTTTTGTTTGAAATTAATATAAAAAAAATCAAATTCCCATCTTTTTGTTGTGGGTTCTTTTTTGTTTCTTCCCCCCCGCATTCAAGCTGGACAAAAAATATGTTGAAAGTCTAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCttat                                                                                                     <  1:655616/249‑1 (MQ=255)                                                                                                                                                   tttGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCttatta                                                                                                   >  1:286946/1‑250 (MQ=255)                                                                                                                                                     tgtgAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTtattat                                                                                                  >  2:198765/1‑249 (MQ=255)                                                                                                                                                      gtgAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatca                                                                                                >  1:51069/1‑250 (MQ=255)                                                                                                                                                       gaaaTGTTTTGGTTAACATTCATAAAAAAAAAATCAAAATCCCATTTGTTTTGTGGGGTTTTTTTTCTGTATCTTCCGCACCGCACACACGCCAGAAAAAAAATATGCTGAAAGCCTAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatca                                                                                                <  2:311374/247‑1 (MQ=255)                                                                                                                                                          aTGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATccc                                                                                            >  1:468389/1‑250 (MQ=255)                                                                                                                                                             ttttggttgACATTAATATAAAAAAATTAATAATTCCATCATTTTTGTGTGGGGTCTTTTCTGTATCTTACGCACCCCACTCAAGCCTGAAAGAAAATTGGCTAAAAGCCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTg                                                                                          <  1:153799/247‑1 (MQ=255)                                                                                                                                                                   ttgACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTg                                                                                   >  1:110891/1‑250 (MQ=255)                                                                                                                                                                      acatacaaagaaaaaaaaTAATAATCCCATCATGTTGGTGGGGGGTTTTTTCTGTATCTTACGCACCCCACACAAGCCTGACAAAAAATATGCTTTAAGCCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGa                                                                                 <  1:152795/238‑1 (MQ=255)                                                                                                                                                                       cttcaataaaaaaaacaTTATAATCCCAACTTTTTGGTGTGGGGTCTTTTCTTATTTTTACGTACCCCCCTCAGGCCTAACAAAAAATGGGCTGTAAGCCTCATTTAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAAc                                                                               <  1:642785/240‑1 (MQ=255)                                                                                                                                                                       cATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGaa                                                                                >  1:258091/1‑249 (MQ=255)                                                                                                                                                                        aTTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACa                                                                              >  2:450463/1‑250 (MQ=255)                                                                                                                                                                           cATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTg                                                                           >  2:335179/1‑250 (MQ=255)                                                                                                                                                                            atatGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGc                                                                          >  2:4884/1‑250 (MQ=255)                                                                                                                                                                            atatGAAAAAAATCAAAGTCCAATCATTTTTGGGGGGGGTTTTTTATGAATTTGACACATCCCACAAAAGACTGACAAAAAAGTGGCGGAAAGGCTCATACAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTg                                                                           <  1:595506/249‑1 (MQ=255)                                                                                                                                                                                      aaTCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTgg                                                                >  2:269288/1‑250 (MQ=255)                                                                                                                                                                                         cATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTa                                                             >  1:773361/1‑250 (MQ=255)                                                                                                                                                                                          aTAATCCCATCATTGTTGGGGTGTGTCTTTTCTGTATCTTACGCAACGCACCCAAGCCAAAAAAAAAATATGCTGAAAGGCTAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTAt                                                            <  2:420461/250‑1 (MQ=255)                                                                                                                                                                                           tAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTAtt                                                           >  1:457194/1‑250 (MQ=255)                                                                                                                                                                                            aaTTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTAtt                                                           >  2:816655/1‑249 (MQ=255)                                                                                                                                                                                                     atGTTGTTGTGGGCTTTTTTCTGTATCTTACGCATCGCACAAAACCCTGAAAAAAAATATGTTGAAAGACTCAAAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATggg                                                  <  2:102062/249‑1 (MQ=255)                                                                                                                                                                                                       ttttGTGGGGTGTTTTTTCTTTATCTTACCCCTCCCACTCAAGCCTAAAAAAAAAGAGGCTGAAAGGCTAATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGc                                                <  1:10004/248‑1 (MQ=255)                                                                                                                                                                                                              tgGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAAt                                        >  2:752614/1‑250 (MQ=255)                                                                                                                                                                                                                  gTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGaa                                     >  2:163062/1‑249 (MQ=255)                                                                                                                                                                                                                   tCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGaaa                                    >  2:673865/1‑249 (MQ=255)                                                                                                                                                                                                                      tttCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGAAAAAAg                                >  2:2153/1‑250 (MQ=255)                                                                                                                                                                                                                      ttcttGTATCTGCCCCCTCGCACAAAAGCCTGAAAAAAAATTTGCTGAAAGGCGCATATAAAAAGCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGaaaaaa                                 <  1:588975/245‑1 (MQ=255)                                                                                                                                                                                                                       ttCTGTCTCTTACGCATCGCACTAAACCCTGAAAAAAAATATGCTGAAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGaaaaa                                  <  1:376072/247‑1 (MQ=255)                                                                                                                                                                                                                        tCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGAAAAAAGcc                              >  1:274155/1‑250 (MQ=255)                                                                                                                                                                                                                                 tACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGAAAAAAGCCCACACAggg                     >  2:672295/1‑250 (MQ=255)                                                                                                                                                                                                                                 tACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGAAAAAAGCCCACACAggg                     >  2:706286/1‑250 (MQ=255)                                                                                                                                                                                                                                    gCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGAAAAAAGCCCACACAggg                     >  1:636172/1‑247 (MQ=255)                                                                                                                                                                                                                                    gCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGCGGAACATTGCCCTTGTTTGGCTCTTCACGCTGCGGCCCACCCATCAAAAAACCCCACCCAcgggcc                  >  2:160933/1‑248 (MQ=255)                                                                                                                                                                                                                                           aCTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGGGGAACATTGCCATTGTTTGGCTATTCAGGCTGGGGCAGACCAATGAAACAAGCCCACACCGGGGAGAggggtc           >  2:513429/1‑248 (MQ=255)                                                                                                                                                                                                                                               aaGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGAAAAAAGCCCACACTGGGGAGAGTGGGCTGa        >  2:713208/1‑249 (MQ=255)                                                                                                                                                                                                                                                aGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGAAAAAAGCCCACACAGGGGAGAGTGGGCTGaaa      >  2:847145/1‑250 (MQ=255)                                                                                                                                                                                                                                                  ccTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGAAAAAAGCCCACACAGGGGAGAGTGGGCTGAAATg    >  1:441262/1‑250 (MQ=255)                                                                                                                                                                                                                                                      aCAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGCGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGAAAAAAGCCCACACCGGGGAGAGCGGGCTGAAATggg  >  2:449386/1‑248 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     ATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGAAAAAAGCCCACACAGGGGAGAGTGGGCTGAAATGGG  >  NZ_CP009273/340767‑341259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑