Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,819,313	0	G	A	32.6%	83.6 / 17.3	49	intergenic (‑119/+84)	ves/spy	environmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy
Reads supporting (aligned to +/- strand):  ref base G (31/2);  new base A (0/16);  total (31/18)
Fisher's exact test for biased strand distribution p-value = 4.57e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTC  >  NZ_CP009273/1819065‑1819558                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                         cgATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑gag‑aagag                                                                                                                                                                                                                                                          >  1:188029/1‑249 (MQ=255)      gatgCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGagaaaag                                                                                                                                                                                                                                                     <  1:312505/249‑1 (MQ=255)      gatgCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGagaaaag                                                                                                                                                                                                                                                     >  1:646900/1‑249 (MQ=255)           aaTGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGACCAGGCTTTTTTTC‑CATCTGTTT‑GAG‑AAGCGAAATGAAAAcc                                                                                                                                                                                                                                               >  2:98644/1‑250 (MQ=255)            aTGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAAccg                                                                                                                                                                                                                                              <  1:100784/250‑1 (MQ=255)               cTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAAccgcc                                                                                                                                                                                                                                            >  1:500157/1‑249 (MQ=255)                       ccAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGCTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTc                                                                                                                                                                                                                                   >  2:635771/1‑250 (MQ=255)                        cAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGTTCCTGTc                                                                                                                                                                                                                                   >  2:457093/1‑249 (MQ=255)                                              gggggAGCGTGCAATTTCCGCGCGTTCCCCCGGCAGCATTCCCCCAGAGATTCCCAGACTTTTTACCCATATAAGCTTATCCAATGCTC‑CCCCCGGCCCTTTTTTTTTCTTGTTTATCTTGCAGGAACCGGTGAATGGTTATGGCAAACGCGGG‑GATTAACCGTTGTTGGTGATCGGGTTTTTTTTA‑‑AACTTTTTAGAA‑AAAAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                                                                                                                            <  2:305398/248‑1 (MQ=255)                                              gTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                                                                                                                            >  1:652781/1‑250 (MQ=255)                                                           aaTTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGCATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGACTATCCTGTCCACCGCATTACTGCAAGGTAGTGGACACGACcggcg                                                                                                                                                                                               >  2:234772/1‑250 (MQ=255)                                                             tttCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGC‑TTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTc                                                                                                                                                                                            >  2:95755/1‑250 (MQ=255)                                                                                        gCCACAGATTCACCGACATTTTACGCATATCAAAGTATCCCATCCTCGCCCCGGGCCGTTTTTTTTTATTT‑TTATCTTTCAGGTACCGGGGGATGTTTATCGACAACTGGGGTGTTTTA‑CTTTAGTGGTGATCAGTTTTTTTTTA‑CAACTGTTT‑GAG‑AAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGattatt                                                                                                                                                                 <  1:494168/250‑1 (MQ=255)                                                                                        gCCAAAGATTCCCCGATATTTTCACAATATCAAAGTATCCCATACGCGCTCCCGGCCGTTTTTTTTCATTG‑TTATCGTTCAGGTACCCGTGGATGTTTATCGGCAACTGGGGTGATTTA‑CGTTAGTGGTGATCAGGTTTTTTTTA‑CAACTGTTT‑GAG‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGattatt                                                                                                                                                                 <  1:364822/250‑1 (MQ=255)                                                                                          cacaGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGattatt                                                                                                                                                                 >  1:660070/1‑248 (MQ=255)                                                                                                ttCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGGTGGAAGATTATTtagacggt                                                                                                                                                         >  2:625281/1‑245 (MQ=255)                                                                                                ttCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGtt                                                                                                                                                         >  1:774164/1‑250 (MQ=255)                                                                                                   aCCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCACGGAAGTGGACAAGACCGGCGGCT‑TGAAATTTTTTGTCTGTAAAattttttcgaagtttga                                                                                                                                                      >  2:690523/1‑239 (MQ=255)                                                                                                           tttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTACGTTTTTTTGGTGAAAGATTATTCATCAGtttctggagtttt                                                                                                                                              >  2:807393/1‑239 (MQ=255)                                                                                                             caCGCATATCAAATTATCCCATCCTCGCTCCCGGCCCTTTCTTTTCATTT‑TTATCTTTAAGGTACCCGTGGATGGTTATCGGCAACTGGGGTTATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTTTTT‑GAG‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTa                                                                                                                                             <  1:235518/248‑1 (MQ=255)                                                                                                               cGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCt                                                                                                                                          >  1:143055/1‑250 (MQ=255)                                                                                                               cGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCt                                                                                                                                          >  1:255346/1‑250 (MQ=255)                                                                                                                 cATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGC‑TTAAGTTTTTTTGCTGAAAAATTTTTTATCGGTTGCAAGCCTTTTTCtttt                                                                                                                                        >  2:114122/1‑250 (MQ=255)                                                                                                                    atCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTTTAGGGATTTTACCGTTTGc                                                                                                                                     >  1:755189/1‑250 (MQ=255)                                                                                                                         attttcccATACTCCCCCCCGCCCTGTTTTTTTCATTT‑TTTTCTTTCGGGAAACGGGGGTTGGTTATCGGCATCTGGGGTGTTTTA‑CTTTAGGGGTGACCGGTTTTTTTTTA‑CACTTTTTT‑GAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTg                                                                                                                                <  1:47446/244‑1 (MQ=255)                                                                                                                                          cccGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAAGTTTTTT‑GCCGGAAGATTTTTTTGCAGTTGCAGGGCTTTTACCTTTTTTCGCCGGGCGTTCTTTTCGACGc                                                                                                               >  2:551081/1‑250 (MQ=255)                                                                                                                                           ccGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGCGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTAATCAGCAGTTGCAGGGCTTTTAACTTTTGCCGCTGGGCGTTCTGTCAGgcgcc                                                                                                              >  1:500964/1‑249 (MQ=255)                                                                                                                                                      ttttCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGC‑TTTAGTTTTTTGGTTGAAAGATTTTTTAGCCGGTGGATGCAAGTTTCATTTCGCCCCGGGGCGTTCCGTCAGACGCGtccccaacatt                                                                                                   >  2:677475/1‑241 (MQ=255)                                                                                                                                                                  aTCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTTAGTTTTTTGGCTGAAAGATTATTCAGCAGGTGCAGGCATTTTACCTTTTTCCGCTGGACGGTCTGTCAGACGCTTCCCAAAATTAGCATT‑TAATTg                                                                                       >  2:757175/1‑250 (MQ=255)                                                                                                                                                                     gTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTTAGACCCTTCTCTAAATTAACATT‑AAAtttttt                                                                                    >  2:467132/1‑246 (MQ=255)                                                                                                                                                                                 gtggcgggtaagcGGCAACTGGGGTGATTTG‑TGTTGGTGTTGATGGGGTTTTTTTTA‑C‑ACCGTTA‑GAGAAAAAAAAAAGAAACCCGCCGAGCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTCTGTTCCgg                                                                        <  1:250008/238‑1 (MQ=255)                                                                                                                                                                                 gTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTCTGTTCCgg                                                                        >  1:786410/1‑250 (MQ=255)                                                                                                                                                                                   ggATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGGTTTTTGGCTGAAAGATTATTTAGCAGTTGGAGGCATTTTACCTTTTGCCGCTGGACGCTCTGTCCGACGGTTCTCAAAATTAGAATT‑AAATTGCTTTTTTTGTTCCGGCg                                                                      >  1:3947/1‑250 (MQ=255)                                                                                                                                                                                   ggATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGATTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGGATTTTACCTTTTTCCGCTGGACGGTCTGTCAGAAGCTTCTCCAAATTAGCATT‑AAATTTCTTTTTTTGTTCCGGCg                                                                      >  2:233640/1‑250 (MQ=255)                                                                                                                                                                                     aTGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGGTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGATGTTCTGTCAGACGCTTCTCAAAATTATCATT‑AAATTGCTTTTTCTGTTCCGGCGTc                                                                    >  1:65275/1‑250 (MQ=255)                                                                                                                                                                                         ttATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTTGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTTTGTTCCGGCGTT‑AGGa                                                               >  1:221763/1‑250 (MQ=255)                                                                                                                                                                                         ttATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAAGTTTTTTGCTGAAAGAATATTTAGCAGTTGCCGGCATTTTTCCTTTTGCCGCCGGACGTTCTTTTACACGCTTCTCCCAATAAACAAT‑TAATTTGTTTTTCTGTTTCGGCGTG‑AGGa                                                               >  2:604370/1‑250 (MQ=255)                                                                                                                                                                                                 aaCTGGGGTTTTTTA‑CGTTGGTGGTGATCAGGTTTTTTTTA‑CAATTTTTG‑GAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTaa                                                       <  1:372877/250‑1 (MQ=255)                                                                                                                                                                                                   ctgggtgtaTTTA‑CGTTGGTGGTG‑TCGTGTTTTTTTTACCCACTTTTG‑AAA‑AAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAAt                                                     <  2:188029/242‑1 (MQ=255)                                                                                                                                                                                                        gTGATTTA‑CTTTAGTGGATATCGTGTTTTTTTTA‑AAACTGTTA‑GAA‑AAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGt                                                <  1:59503/250‑1 (MQ=255)                                                                                                                                                                                                         tgctttA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTG‑TTAAGTTTTTTCGCTGCAAGATTACTTAGCAGTTGTAGGCGTTTTACCTTTTGCCGCTGGACGTTCTTTCAGACGCCTCTTAAAACTTGCAGT‑AACTTGCTTTTTTTTTTCTGGCGGC‑AGGATGTTGTAACTtttttt                                               >  2:785815/4‑250 (MQ=255)                                                                                                                                                                                                                 cGTTAGTGGTGATCAGGCTTTTTTTA‑AAACTGTTT‑GAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGtt                                        <  1:556113/250‑1 (MQ=255)                                                                                                                                                                                                                    ttgggtggtaaaGGTTTTTTTCA‑CAATTGTTG‑GAA‑AAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTcc                                     <  1:330823/239‑1 (MQ=255)                                                                                                                                                                                                                    tAGTGGTGACCAGTTTTTTTTTA‑CAATTGTTT‑GAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGtt                                        <  1:629927/247‑1 (MQ=255)                                                                                                                                                                                                                              cAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCC‑TTAAGCTTTTTGGGTCGAAAATTAATCAGCAGCTGGAGGCCTTTTTACTTTTGCCGCTGGGCCGTCTCTTAAACCCTTCTTTCAATTAGTATTAAAATCGTTTTTTTTGTT‑CGGCGTCTAGTGGGTTTAAAATTTTTTTTGGGGTTTctgtgtctc                              >  2:416897/1‑240 (MQ=255)                                                                                                                                                                                                                               agttttttttcA‑AAATTTTTT‑GAA‑AAAAAAAAAAAAAGCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAg                          <  1:33372/246‑1 (MQ=255)                                                                                                                                                                                                                                ggCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAg                          >  1:52827/1‑249 (MQ=255)                                                                                                                                                                                                                                 tcTTTTTTCA‑CTGCTTTTA‑GAG‑AAAAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGc                         <  2:156963/248‑1 (MQ=255)                                                                                                                                                                                                                                  cTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCAt                       >  1:401203/1‑250 (MQ=255)                                                                                                                                                                                                                                           acatatGTAT‑GAA‑AATAAAAAAGAAAGCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGCTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCt                <  1:419214/244‑1 (MQ=255)                                                                                                                                                                                                                                            cAACTGTAT‑AAA‑AAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCttt              <  2:193510/250‑1 (MQ=255)                                                                                                                                                                                                                                              attGTTT‑GAA‑AAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTgc            <  1:153985/248‑1 (MQ=255)                                                                                                                                                                                                                                                       ag‑aagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTACGTTTTTTGGCTGAAAGATTATTCAGCAGGTGCAGGCATTTTACCTTTTGCCCCCGGACGCTTTGTCAGACGCTTCTCAAAATTTACATT‑AAATTGCTTTTTTTTTTCCCGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCATTTTTGCCCGCATGCTTGCTTTTCGCtttttt    >  2:765371/1‑248 (MQ=255)                                                                                                                                                                                                                                                          aagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGGTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTTCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTTCGCCAGCATGTTAGCTTTGCGCTGttcttc  >  2:120596/1‑250 (MQ=255)                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                         CGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTG‑TTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTA‑CGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTT‑GAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT‑AAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTC  >  NZ_CP009273/1819065‑1819558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑