Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,820,6360TC32.1% 97.0 / 12.0 56E182G (GAG→GGG) astEsuccinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base T (29/9);  new base C (1/17);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 8.56e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACT‑GGCGA‑AGATCGTTTTGCCCAAAGGG‑CAA‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTC  >  NZ_CP009273/1820388‑1820875
                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                
cGAAGGACGGCGAGTGGCGAGTAAGTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACT‑GGCGA‑AGATCGTTTTGCCCAAAGGG‑CAA‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCt                                                                                                                                                                                                                                                  <  2:289367/249‑1 (MQ=255)
        ggcgagtggcgagtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACT‑GGCGA‑AGATCGTTTTGCCCAAAGGG‑CAA‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCccg                                                                                                                                                                                                                                        <  2:537425/250‑1 (MQ=255)
                                       gATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTTCAAACT‑GGCGA‑AGAACGGTTTGCCCAAAGGG‑CAA‑CGCTTTGTCCAGTT‑TCCGCGGAC‑AGGGCCGCGCGCCAAAATGTCTCGCGCCGAAATGGG‑TGAA‑CCTAACAACCG‑GGTTCT‑GAATGGAA‑ACCCCCG‑CCCCCCG‑GCCCGCCGCCCCCACCCACGTCCGAAAATTCTCgcc                                                                                                                                                                                                         >  2:325793/1‑248 (MQ=255)
                                         tAACGGAGCGGCGGTGTTCTCACGATACCGCCACTCCCACCAGAAAGGAGCGCAGCAAATGCGCTGGCCGTTACTGCAAAAA‑GGCGA‑AGATACTTTTTTCCAAAAGG‑CAA‑CGCTTTTCCAAGTT‑CCACCGTAC‑AGGCCAGCGCGCCAAAATATCCGGCGCTGCAAATGG‑TAAA‑AGG‑CACACCA‑CGGTCC‑CAATGTGACACCAGCG‑CCTCTCA‑CCCCCCCGCCCCCAACCACGCCACAAATTtctcacccc                                                                                                                                                                                                       >  2:385305/1‑245 (MQ=255)
                                            gggggcggcgGTTTCCCCACGATCCCGACACCCCCACCAGAAAGCAGCGCAGCAATTGCGGTGGCAGTTACTACAAACG‑GGCGA‑AGATCTTTTTGCCAAAGGGA‑CAA‑CTCTTTGCAAAGTC‑CAAGCGAAC‑GGCCCAGCGCGCAAAAATGTCGGGCGCAGAAAGGGG‑AAAC‑CGC‑CCCACCG‑GTTTCC‑GGAGGAAACCCCGGCC‑CCCCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAgg                                                                                                                                                                                                    <  2:259273/246‑1 (MQ=255)
                                                   gcggTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACT‑GGCGA‑AGATCGTTTTGCCCAAAGGG‑CAA‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCCCCCAGCCACGTCCGAAATTTCTCGTCCCAGGGAATgcc                                                                                                                                                                                             >  2:66922/1‑248 (MQ=255)
                                                           ctcACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACT‑GGCGA‑AGATCGTTTTGCCCAAAGGG‑CAA‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAACTTTCTCGTCCCAGGGAATGTCGCGCTGCg                                                                                                                                                                                     >  2:174061/1‑250 (MQ=255)
                                                             cccGACACCGCCCCCCTCCCAAGATAGCAGCGAAGAAATGGCG‑TGGACGTTTCCGCAAACC‑GGGGA‑AAGTCTTTTTTGCCAAAGGG‑GAACCGCTTTCCAAATTC‑CCAGCGTAC‑AGGCCGGCGCGCAAAAATGTCGGGCGCAAAAATGGA‑AAAA‑CGC‑CCCCCCA‑GTTCCC‑TGAGGAACCCCCACCC‑CCCCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGt                                                                                                                                                                                   <  2:457887/248‑1 (MQ=255)
                                                                   aCCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACT‑GGCGA‑AGATCGTTTTGCCCAAAGGG‑CAA‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATaca                                                                                                                                                                             >  1:177864/1‑250 (MQ=255)
                                                                   aCCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACT‑GGCGA‑AGATCGTTTTGCCCAAAGGG‑CAA‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGCTGCGGTAATaca                                                                                                                                                                             >  2:53627/1‑250 (MQ=255)
                                                                    ccGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACG‑GGCAA‑AGATCGTTTTGCCAAAAGGG‑CAA‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCAAAAATGTCTGGCGCTGAAATGGT‑TAAA‑CGT‑ACCCCCA‑GTTTCC‑TGATGAAACCCCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATaca                                                                                                                                                                             <  1:693058/249‑1 (MQ=255)
                                                                               cccAGAGAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACG‑GGCGA‑AGATCGTTTTGCCAAAAGGG‑CAA‑CGCTTTGCCAAGTC‑CCAGCGTAC‑AGGCCAGCGCGCAAAAATGTCGGGCGCTAAAATGGG‑TAAA‑CGT‑CCCCCCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGc                                                                                                                                                                    <  1:291700/246‑1 (MQ=255)
                                                                                                 aaTTGCGCTGGCAGTTACTGCAAACT‑GGCGC‑AGATCGTTTTGCCCAAAGGG‑CAA‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAACTGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACCGCGGATGCAAGGAGCCACGCAtt                                                                                                                                               >  2:65188/1‑250 (MQ=255)
                                                                                                 aaTTGCGCTGGCAGTTACTGCAAACT‑GGCGA‑AGATCGTTTTGCCCAAAGGG‑CAA‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATCAACCGAACTGCGGATGCAAGGAGCCACGAAtt                                                                                                                                               >  2:254048/1‑250 (MQ=255)
                                                                                                  aTTGCGCTGGCAGTTACTGCAAACT‑GGCGA‑AGATCGTTTTGCCCAAAGGG‑CAA‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGCTCC‑TGATGGCACACCAGCG‑CCTCCAG‑CCCCCCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGACTGTCGCGCTTCGGTAATACACCGAACTGCGGACGACAGGAGCCACGAATTg                                                                                                                                              >  2:502524/1‑250 (MQ=255)
                                                                                                   ttGCGCTGGCAGTTACTGCAAACT‑GGCGA‑AGATCGTTTTGCCCAAAGGG‑CAA‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCTCCGTCAGCAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGCACGCAAGGAGCCACGAATTGc                                                                                                                                             >  2:176628/1‑250 (MQ=255)
                                                                                                           gCAGTTCCTGAAAACT‑GGCGA‑AGATCGTTTGGCCAAAAGGG‑CAC‑CGCTTTGCCAAGTC‑CCAGCGTAC‑AGGCCAGCGCGCAAAAATGTCTGGCGCTGAAAGGGG‑TAAC‑CGT‑CCCCCCA‑GTTCCC‑TGAGGGAACACAAGCG‑CCCCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTa                                                                                                                                     <  1:664734/250‑1 (MQ=255)
                                                                                                           gCAGTTACGGCAAGCT‑GGCAA‑AGTGCGTTTTGCCCAAGGGC‑CAA‑CGCTTTGCCAAGTC‑CCAGCGAAC‑AGCCCCGCGCAAAAAAATGTCCGGCGCGAAAATGGA‑AAAA‑CGC‑ACCCCCG‑GGTTCC‑TGATGAACCACCCGCG‑CCCCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTa                                                                                                                                     <  2:69925/250‑1 (MQ=255)
                                                                                                               gttgctgaaaCTGGGCAA‑AGAT‑GTTTTGGCCAAAGGG‑ACA‑CCCTTTTCCAAAGT‑CCCGGCTACAAGGCCCGC‑CCCAAAAATGTCCGGGCGTGAAATGGG‑TAAA‑CCG‑CCCCCCA‑GGGTCCTTGAGGAAACCCCCCCC‑CCCCCGG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGa                                                                                                                                   <  2:626438/242‑1 (MQ=255)
                                                                                                                 aCTGAAAACT‑GGCGA‑AGATCGTTTTGCCCAAAGGC‑CAC‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCAAAAATGTCGGGCGCTAAAATGGG‑TAAA‑CGT‑CCCCCCA‑GGTTCC‑TGATGAAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCa                                                                                                                                <  1:401333/249‑1 (MQ=255)
                                                                                                                  cTGCAAACT‑GGCGA‑AGATCGTTTTGCCCAAAGGG‑CAA‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCGGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAg                                                                                                                              >  2:198578/1‑250 (MQ=255)
                                                                                                                   tGCAAACT‑GGCGA‑AGATCGTTTTGCCCAAAGGG‑CAA‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAgg                                                                                                                             >  1:621832/1‑250 (MQ=255)
                                                                                                                   cgCAAACC‑GGGGACAGTTCGTTTGACCAAAAGGG‑CAA‑CGTTTTGCCAGGTC‑CCAGCGAAG‑AGGCCGGCGCCAAAAAATGTGTGGCGCTAAAAGGGT‑TAAC‑CGC‑CCCACCG‑GTGCCC‑TGAGGGAACCCCAGCC‑CCCCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCaa                                                                                                                               <  2:457803/248‑1 (MQ=255)
                                                                                                                      aaaCC‑GGGGA‑AGGACGGTTTGCCCCAAGGG‑CAA‑CCCTTTGGCAAGTTGCCAGC‑TAC‑AGGCCCGCGCGGCAAAATGGCTGGGCCCGAAAAGGG‑TAAACCGA‑CCCCCCA‑GGTTCT‑TGAT‑GAACAACCGCC‑CCCCCCGCCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTg                                                                                                                           <  2:392176/250‑1 (MQ=255)
                                                                                                                         cT‑GGCGA‑AGATCGTTTTGCCCAAAGGG‑CAA‑CGCTTTGCAAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCAAAAATGTCGGGCGCTGAAAGGGG‑TAAA‑CGT‑ACCACCA‑GGTCCC‑GGATGAAACACCAGCG‑CCCCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAg                                                                                                                       <  1:791710/250‑1 (MQ=255)
                                                                                                                                    aTCGTTTGCCCAAAAGGC‑CAA‑CTCTTTGCAAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCAAAAATGTCGGGCGCTGAAAGGGG‑AAAA‑CGT‑ACCACCG‑GGTTCC‑TGATGAAACACCAGCG‑CCCCCAC‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGAt                                                                                                              <  1:234528/250‑1 (MQ=255)
                                                                                                                                            gCCCAAAGGG‑CAA‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGCTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGCATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGcc                                                                                                      >  2:693404/1‑250 (MQ=255)
                                                                                                                                             cccAAAGGG‑CAA‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGGTTCTTTGccc                                                                                                     >  2:13435/1‑250 (MQ=255)
                                                                                                                                               aaaaGGGCCAG‑CGCTTTGCAAAGTG‑CGCGCGCAG‑AGGCCGGCGCGACAAAATGTC‑GGGGCGAGAATGGGATAAA‑CGT‑CCCACCA‑GGTTCC‑TGAGGGAACCCCCGCC‑CCTCCAC‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCt                                                                                                    <  2:378670/249‑1 (MQ=255)
                                                                                                                                                       ccaccGCTTGGCAAACTG‑CCAGCGTCC‑AGGCCAGCGCGCAAAATTGCGTGGCG‑TGAAAAGGG‑GAAACCCC‑CCCCCCA‑GTTTCC‑GGATGGACCACCGGCG‑CCCCCGG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATa                                                                                             <  2:341671/246‑1 (MQ=255)
                                                                                                                                                            gCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGCTCAAGGTGCCAGCGCACAGATTCTTTGCCTCGGTCATAAAAAt                                                                                        >  2:413442/1‑250 (MQ=255)
                                                                                                                                                            gCTTTGCCAAGTC‑CAAGCGTAC‑AGGCCGGCGCGCAAAAATGTCGGGCGCTGAAATGGA‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGAGGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAAt                                                                                        <  1:670049/250‑1 (MQ=255)
                                                                                                                                                               ttGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGAAGATCAAGGAGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCtt                                                                                     >  2:548938/1‑250 (MQ=255)
                                                                                                                                                                tGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTc                                                                                    >  1:710446/1‑250 (MQ=255)
                                                                                                                                                                          ccAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATACACATCTTCCAGGCACTg                                                                           >  2:67538/1‑250 (MQ=255)
                                                                                                                                                                          ccACCGTAC‑GGCCCACCGCGCAAAAATGTGTGGCGCTGAAATGGG‑AAAA‑CTC‑CCCACCA‑GGTCCC‑TGAAGGAACACCAGCC‑CCCCCGG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACt                                                                            <  1:670893/249‑1 (MQ=255)
                                                                                                                                                                                     gcccAGCGCGCAAAATGGTCGGGCGCTGAAAGGGG‑AAAA‑CGT‑CCCACCA‑GTTCCC‑TGAGGAAACCCCAGCG‑CCCCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTcg                                                                 <  1:466303/248‑1 (MQ=255)
                                                                                                                                                                                      gCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTcgc                                                                >  2:841695/1‑250 (MQ=255)
                                                                                                                                                                                       ccgggcgcacAAAAGGGCTGGCGCGAAAAGGGG‑TAAA‑CTG‑CCCACCA‑GGTTCC‑TGATGGAACCCCCGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTcgcg                                                               <  1:431970/241‑1 (MQ=255)
                                                                                                                                                                                           cgcgcCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGcc                                                           >  2:427312/1‑250 (MQ=255)
                                                                                                                                                                                            gcgcAAAAAGGTCTGGGCATGAAAGGGG‑AAAC‑CGC‑ACCCCCG‑GGTCCG‑GGATGAAACCCCAGCC‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGccc                                                          <  1:490603/250‑1 (MQ=255)
                                                                                                                                                                                                             gcTGAAAGGGG‑AAAC‑CGC‑CCCCCCA‑GGTCCT‑GGAGGAAACCCCAGCG‑CCCCCCG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCtt                                         <  1:376565/250‑1 (MQ=255)
                                                                                                                                                                                                                   aTGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGc                                    >  1:644541/1‑249 (MQ=255)
                                                                                                                                                                                                                    ttgg‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCa                                   >  1:135989/3‑249 (MQ=255)
                                                                                                                                                                                                                    gggg‑gAAA‑CGT‑CCCCCCCGGGTTCC‑TG‑TGGGACCCCCGCGCCCCCCAC‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCa                                   <  2:139807/249‑1 (MQ=255)
                                                                                                                                                                                                                         aaaa‑CCT‑CCCCCCA‑GTTTCC‑TGATGAACCCCCCGCG‑CCCCCCC‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAg                              <  2:644541/249‑1 (MQ=255)
                                                                                                                                                                                                                                    cacca‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAAc                      >  1:656360/1‑249 (MQ=255)
                                                                                                                                                                                                                                        catgTTCC‑GGATGAACCCCCGGCC‑CCTCCAG‑CTCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGAcc                  <  1:423545/247‑1 (MQ=255)
                                                                                                                                                                                                                                        a‑GGTTCC‑TGATGGAACACCAGCG‑CCTTCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCg                 >  1:73769/1‑250 (MQ=255)
                                                                                                                                                                                                                                        a‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCg                 >  2:231593/1‑250 (MQ=255)
                                                                                                                                                                                                                                           gTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTACAGCAAATAGCTGCCAACGACCGc                >  1:161016/1‑249 (MQ=255)
                                                                                                                                                                                                                                             tCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCa              >  1:318910/1‑249 (MQ=255)
                                                                                                                                                                                                                                              cc‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCaaa            >  2:402012/1‑250 (MQ=255)
                                                                                                                                                                                                                                                    gggacccccAGCC‑CCCCCCG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTc       <  1:121640/243‑1 (MQ=255)
                                                                                                                                                                                                                                                     ggAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGTTGCCAACGACCGCCAAACATTCg      >  2:107735/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       aaCACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCAAACCATTCGAt    >  2:39467/1‑250 (MQ=255)
                                                                                                                                                                                                                                                         cacCAGCC‑CCCCCAC‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTc  <  1:290067/250‑1 (MQ=255)
                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                
CGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACT‑GGCGA‑AGATCGTTTTGCCCAAAGGG‑CAA‑CGCTTTGCCAAGTT‑CCAGCGTAC‑AGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGG‑TAAA‑CGT‑ACCACCA‑GGTTCC‑TGATGGAACACCAGCG‑CCTCCAG‑CCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTC  >  NZ_CP009273/1820388‑1820875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑