| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,089,571 | 0 | A | G | 31.8% | 114.7 / 15.1 | 63 | I83V (ATT→GTT) | hisF | imidazole glycerol phosphate synthase subunit HisF |
| Reads supporting (aligned to +/- strand): ref base A (6/37); new base G (20/0); total (26/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.71e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATGCTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG‑ACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACA > NZ_CP009273/2089324‑2089815 | cATGCTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAGGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGAtt < 2:88538/250‑1 (MQ=255)cATGCTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGAtt > 1:246609/1‑250 (MQ=255) ggCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑Ggct > 1:366385/1‑250 (MQ=255) cAAAACGCATAATCCCATGGCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTctct < 2:433996/250‑1 (MQ=255) aaCGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTTGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGa < 1:647517/250‑1 (MQ=255) gCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTGA‑GTCTCT‑GGGAgt > 1:30782/1‑249 (MQ=255) ccATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGGTAA‑GTGTCT‑GGGAGAAGGcgcg > 2:584920/1‑249 (MQ=255) aTGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGaaa < 1:109758/250‑1 (MQ=255) gTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑t < 1:466201/250‑1 (MQ=255) gTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGAACGACATTCCGTTTTGTGTGGCGGGGGGGGTTGA‑GTGTCT‑GGAAGAGGCCCCGAGAAGTCTTTTC‑t > 2:749185/1‑250 (MQ=255) gTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTg < 1:584738/249‑1 (MQ=255) tGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGgc < 1:584920/250‑1 (MQ=255) gTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTTA‑GACTCT‑GGAAGATGCCGCGCAAAATTTTTCC‑TTTGGGGCggg > 1:694367/1‑249 (MQ=255) tCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGGAA‑AGCTCTGGGAAAAGGCCCGGAAAATTTTTTCT‑TTTgggcggg > 2:794444/1‑244 (MQ=255) aGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATaa < 1:837695/250‑1 (MQ=255) ggtggtGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATaaa < 1:433803/250‑1 (MQ=255) aaaGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTGAAGGTTTCT‑GGAAGA‑GGCGCGGAAAATCTTTCC‑TTTTGTGCGGGATAAAtttac > 2:763076/1‑247 (MQ=255) aaGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCAt < 1:296322/250‑1 (MQ=255) aaGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTTA‑GTGTCTCGGAGGAGGCC‑CGCAAATTTTTTCCTTTTGCCGCGGGAAAAATTTcct > 2:141257/1‑249 (MQ=255) atgCGTGCAGTTTCGCAACCATGGAATCAGTGGGGATATCGTGCCGTTGGCAAAACGCTAAGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATc < 2:481833/248‑1 (MQ=255) aGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGGAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATc < 1:568635/250‑1 (MQ=255) tACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGAGTAA‑GTCTCT‑GGAAGAAGCCGCGAAAACTCTTTTC‑TTTTGCGCGGGTAAAAATTTCATCAActc > 1:173252/1‑250 (MQ=255) cAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACtctc < 1:60358/250‑1 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAA‑GTATCT‑GGAAGATGCCGCGAAAATTTTTTCC‑TTTTGCGCGGGTAAAAATTTCATCAACTCTCCTtcg > 1:233451/1‑250 (MQ=255) cATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAg < 1:520304/250‑1 (MQ=255) aTCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGGTAA‑GACGCTGGGAAGA‑GACGGGAAAAATCTTTCC‑TTTTGCGGGGTGAAAATCTTCCTCAACTCTCCTGCGCCGGGAGAACCaa > 2:675743/1‑250 (MQ=255) tGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTTC‑TTTGGCGCGGGTAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCACCatt > 1:7020/1‑249 (MQ=255) cGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACattaatt < 2:8288/250‑1 (MQ=255) gggaaaaCGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTGCGAGATCACCGCTAACAGCAATGGCCGTGTGGTAGATAAAAGCTGGGTAACTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGTAGACCCAACATTAATTACTCGCCTGGCCGAt < 1:124089/247‑1 (MQ=255) acgctacgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑Cttt < 1:539241/250‑1 (MQ=255) tacgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTTTTTTC‑TTTGGTGCGGGTAAAATTTCCATTAACTCTCCCGCCCTGGCCGACCCCACATTAATTACTCTCCTGGCCGCACG‑CTTTTGCg > 2:130366/1‑250 (MQ=255) cgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCg < 2:547069/248‑1 (MQ=255) ctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTA‑GTCTCT‑GGAAGATGCCGCGAAAAATTTTTCC‑TTTGGCGCGGGTAAAATTTTCATCAAATCTCCTTCGCTGGCCGACCCAACATTAATTACTCCCCTGGCCGATCGCCTTTggggggc > 1:588871/1‑245 (MQ=255) tGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACCTTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAg > 1:327653/1‑250 (MQ=255) gaagGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTa < 1:112042/250‑1 (MQ=255) aagGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTTC‑TTTGGCGCGGGTAAAATTTCCATCAACTCTCCTGCGCTGGCCGACCCAACATTAATTACTCGCCTTGCCGATCG‑CTTTTGCGTGCAgtgttt > 1:742749/1‑248 (MQ=255) gcgcTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATtgt < 1:782187/250‑1 (MQ=255) gcTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTgg < 2:313801/250‑1 (MQ=255) gcTGACGAACTGGTGTGCTACGATATCACCGCTGCCAGCGATGGCCGTGGGGTAGATAAAAGCTGGGTAGCTCGCGTGGCGGAAGTGAGCGACATTCCGTTTTGTGTGGCGGGTGGGAGTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTgg < 2:319177/250‑1 (MQ=255) aaCTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTA‑GTCTCT‑GGGAGAAGCCGCGCAAAATTTTTTC‑TTTTGCGCGGGTAAAAATTCCATCAACTCTCCTTCGCTGGCAGACCCAACATTAATTACTCGCCCGGCCCGTCG‑CTTTTGCGTGCCGTGTATTGTgggtgggtt > 2:352650/1‑243 (MQ=255) ttCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCt < 2:384799/250‑1 (MQ=255) gCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAA‑GTCTCT‑GGGAGATGCCGCGAAAATTCTTTCC‑TTTTGCGCGGATAAAATTTCCATCAACTCTCCCGCGCTGGCAGACCCCACATTAATTACTCGCCTGGCCGATCG‑CCTTGGCGTGCAGGGTATTGTGGGCGGTATTGATACCTGGTACG‑ACGCCgga > 2:207297/1‑247 (MQ=255) gCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTTA‑GTCTCT‑TGGAGAAGCCGCGGAAATTTTTTTC‑TTTTGCGCGGATAAAATTTTCCTCAACTCTCCTGCGCCGGCCGACCCAACATTAATTATTCGCCCTGCCGATCG‑CTTTGGCGTGCAGTGGATTTTGGTCGGTTTTGTTACCTGGTACG‑CCGCCGaa > 2:539741/1‑249 (MQ=255) ccAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG‑ACGCCGAAAcc < 2:16341/248‑1 (MQ=255) cAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAA‑AGCTCT‑GGGAGATGGCCCCAAAATTCTTTTC‑TTTGGCGCGGATAAAATTTTCAACAAACCTCCCGCGCTGGGAGACCCCACATTAACTACTCCCCCCGCCGATCG‑CCTTTGCGTGCCGGGTTTTGAGGGCGGGGTTGATACCCTGGACG‑ACGCCgcacaccgt > 2:788893/1‑242 (MQ=255) gCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTTGCGCGGATAAAATTTTCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG‑ACGCCGAAACCGGTAAATATCa > 1:166718/1‑250 (MQ=255) tgtgGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG‑ACGCCGAAACCGGTAAATATCAtgtg < 1:265041/250‑1 (MQ=255) gTAGATAAATGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG‑ACGCCGAAACCGGTAAATATCATGTGAAt < 2:21580/249‑1 (MQ=255) tAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG‑ACGCCGAAACCGGTAAATATCATGTGAATCa < 2:246609/250‑1 (MQ=255) cTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGGAAATTCTTTCC‑TTTTGCGCGGATAAAATTTTCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGGATTGATACCTGGTACG‑ACGCCGAAACCGGGAAATATCATGTGAATCAATATAACGGc > 1:656179/1‑250 (MQ=255) cTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAA‑AGTTCT‑GGGAGAAGGCGCGAAAAATCTTTTC‑TTTGGGGCGGGTAAAATTTCCATCAACTCCCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGCTCG‑CTTTTGCGGGCCGCGTATTGTGGTCGGGCTTTATACCTGGTGCG‑ACCCCGAAACCGGGAAATATAATGTGAATAAATATACAGGc > 2:155565/1‑250 (MQ=255) ggTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG‑ACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGAt < 2:854637/250‑1 (MQ=255) ggTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG‑ACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGAt < 1:562022/250‑1 (MQ=255) gatATCGCGCGTGGCGGAAGTTGTCGACATTCCGTTTTGGGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG‑ACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGTCGAt < 2:128687/248‑1 (MQ=255) ctcGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTTA‑GTCTCT‑GGGAGATGGCCCGAAAATTTTTTTC‑TTTTGCGCGGGGAAAATTTTCATCAACCCTCCTGCGCTGGCCGAACCCACATTAATTAATCGCCCGGCCGCTCG‑CTTTGGCGTGGCGTGTATTTTGGTTGGTATTGGTACCCGGGACGCACCCCGAAA‑CGGGAAATATTATGTGAATCAATAAACCGGcgacga > 2:712967/1‑247 (MQ=255) cgcgTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAA‑GTTCCT‑GGTAGATGCCGCGCAAAATCTTTCC‑TTTGGCGCGGGTAAAATTTCCATCAACACTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCCATCG‑CTTTTGCGGGCAGGGGATTTTGGGCGGTAGTTATACCCGGGACG‑ACGCCCCAACCCGTAAATATCATGTGAATCAATATACCCGCGGCGAAAAcc > 2:663319/1‑250 (MQ=255) gcgTGGCGGAAGTGATCGACATTCCGGTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG‑ACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGaaa < 1:464412/246‑1 (MQ=255) tGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG‑ACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCAc < 2:614433/250‑1 (MQ=255) aTCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG‑ACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACt < 1:763076/249‑1 (MQ=255) tgtgtgGCGGGTGTGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTGGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG‑ACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTc < 2:168038/249‑1 (MQ=255) gtgtgGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG‑ACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCg < 1:469393/249‑1 (MQ=255) tgGCGGTTGGAATTAA‑TTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGTCATACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG‑ACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACt < 2:217813/249‑1 (MQ=255) gTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG‑ACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACa < 1:207297/250‑1 (MQ=255) | CATGCTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAA‑GTCTCT‑GGAAGATGCCGCGAAAATTCTTTCC‑TTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCG‑CTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG‑ACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACA > NZ_CP009273/2089324‑2089815 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |