Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	659,964	T→G	D228A (GAT→GCT)	rlpA ←	endolytic peptidoglycan transglycosylase RlpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	659,964	0	T	G	100.0%	198.8 / NA	64	D228A (GAT→GCT)	rlpA	endolytic peptidoglycan transglycosylase RlpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/37);  total (27/37)

GCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACGGTCAGCTGCCGCGCGAGAAAGTG  >  NZ_CP009273/659719‑660207                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    gCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTc                                                                                                                                                                                                                                                 >  1:806870/1‑250 (MQ=255)        gTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCtttt                                                                                                                                                                                                                                           <  2:583127/249‑1 (MQ=255)         tACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCtttt                                                                                                                                                                                                                                           >  1:242045/1‑248 (MQ=255)          aCTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAg                                                                                                                                                                                                                                        >  2:630815/1‑250 (MQ=255)           cTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAg                                                                                                                                                                                                                                        <  2:545502/249‑1 (MQ=255)              ttGCGCCCGAGCCTGTGCGCTTACGGCCCCGACTTGAACCATAAAGTGGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTc                                                                                                                                                                                                                                    <  2:806108/250‑1 (MQ=255)                       aGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTg                                                                                                                                                                                                                           <  1:365132/250‑1 (MQ=255)                          cggATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGAcc                                                                                                                                                                                                                        <  2:633290/248‑1 (MQ=255)                              tCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGgaa                                                                                                                                                                                                                    <  1:534019/250‑1 (MQ=255)                                      ggCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCa                                                                                                                                                                                                            <  1:779202/250‑1 (MQ=255)                                       gCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCAc                                                                                                                                                                                                           <  1:847623/250‑1 (MQ=255)                                         cccGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAAGGTCAccc                                                                                                                                                                                                         >  2:619796/1‑250 (MQ=255)                                          ccGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGCACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGATTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCt                                                                                                                                                                                                        >  1:61675/1‑250 (MQ=255)                                             aCTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCg                                                                                                                                                                                                     <  1:21081/250‑1 (MQ=255)                                              cTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCgg                                                                                                                                                                                                    <  1:261191/250‑1 (MQ=255)                                                 gCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGCAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGc                                                                                                                                                                                                  >  2:3156/1‑249 (MQ=255)                                                       tAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGGGCTTCGCTTTTTAGCGTCGACTTACTGACCGGAAGAATGTCCCCCCGCGGGCCAGacac                                                                                                                                                                                           >  2:122927/1‑250 (MQ=255)                                                                        cTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCgcgc                                                                                                                                                                          >  2:432871/1‑250 (MQ=255)                                                                         tttGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGcc                                                                                                                                                                         >  2:814891/1‑250 (MQ=255)                                                                         gttggtggaCGGGTTGGGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGcc                                                                                                                                                                         <  2:517852/243‑1 (MQ=255)                                                                             cGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCAcc                                                                                                                                                                      <  2:362294/249‑1 (MQ=255)                                                                                    gCTGGCGGTGTCACCATTGCAGTCGGGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAAt                                                                                                                                                              <  2:637922/250‑1 (MQ=255)                                                                                        gCGGAGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGGGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCCGCACCGCTTAAATCggg                                                                                                                                                          >  2:286951/1‑250 (MQ=255)                                                                                          ggTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCgggcg                                                                                                                                                        >  1:481384/1‑248 (MQ=255)                                                                                           gtgtCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAgg                                                                                                                                                       <  1:672680/250‑1 (MQ=255)                                                                                               cACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTg                                                                                                                                                     >  1:289508/1‑248 (MQ=255)                                                                                               cACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGCCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGGGGTg                                                                                                                                                     >  2:139197/1‑248 (MQ=255)                                                                                                aCCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTgca                                                                                                                                                   <  1:198074/249‑1 (MQ=255)                                                                                                             aGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGt                                                                                                                                     >  1:306775/1‑250 (MQ=255)                                                                                                             aGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGt                                                                                                                                     <  1:482528/250‑1 (MQ=255)                                                                                                                   cAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGCATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAACCGGGAGGCGCAGGCAGGGCGTAAGGCTGttt                                                                                                                               >  2:714612/1‑250 (MQ=255)                                                                                                                     ggggTTGTCGACGGGGCTGGAACAACGGGCTGTGGCGCAGGGGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTgg                                                                                                                             <  2:690107/247‑1 (MQ=255)                                                                                                                                 ggAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTAc                                                                                                                 <  1:609816/250‑1 (MQ=255)                                                                                                                                   aGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACaa                                                                                                               <  1:469031/250‑1 (MQ=255)                                                                                                                                    gCTGTAACAACGGGCTGGGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAg                                                                                                              <  2:409563/250‑1 (MQ=255)                                                                                                                                        tAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCa                                                                                                           >  1:609186/1‑249 (MQ=255)                                                                                                                                                 ggtgtgtgggaGGGGTCGGTGCGCTGCCTTCCAGTACCCCGGGGGCTAAGGTCGTGGGTGCGCCGAGGAAGCCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTGGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGAcc                                                                                                 <  2:372569/240‑1 (MQ=255)                                                                                                                                                      ggCGCGGGCGCCGGTCCGCGGCCTCCCAGTCCACCGGGCGCAAGGGTGGTTGGTGGGCGGAGGAAACCGCTACTGGTTACCGGCGGGCCGGTCGGAGCTTCGCTTTTTAGCGTCAAATTACTGACCGGAAGAATGTCACCGTGCGGGCCAGACACGGAACTTGTTCCCGCGCCGCCGCTTAAATCGGGGGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGaaa                                                                                            <  2:372860/250‑1 (MQ=255)                                                                                                                                                      ggCGCAGGGGTCGGTTCGCTGCCTGCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGaaa                                                                                            <  1:119729/250‑1 (MQ=255)                                                                                                                                                      ggCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGGCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGTTGCAGGCAGGGCGTAAGTCTGTTGGGCGACTGTGGTACAAGCCATACCAGGACCAGaaa                                                                                            <  1:620936/250‑1 (MQ=255)                                                                                                                                                         gcAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCGAAGGTCGTTGGTGCGCCGAGGAACCCGCTACTGGTTACCGGCGCGCCGGCCGGAGCTTCGCTTTTTAGCGTCGAATTACGGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCg                                                                                         <  1:250596/250‑1 (MQ=255)                                                                                                                                                           aGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCCCCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGaa                                                                                       >  1:699298/1‑250 (MQ=255)                                                                                                                                                              ggtgggTCCGCTGCCTTCCAGTCCCCCGGGCGCTAGGGTCGTTGGTGCGCGGGGGAACCCGCTACGGGTTCCCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCa                                                                                    <  2:217496/246‑1 (MQ=255)                                                                                                                                                              cggcGGTCCCCTGCCTCCCGTTCCACCGGCCGTTAGGGTCTTGGGGGCGCGGAGGAACCCGCTACTGGTTCCCGGGGCGCCGGTGGGGGCTTCGTTTTTTGGCGTCGAATTCTTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCCCCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCa                                                                                    <  2:658682/247‑1 (MQ=255)                                                                                                                                                                   gTTCGCTGCCTTCCAGTACACCGGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACGGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTg                                                                               <  1:637353/250‑1 (MQ=255)                                                                                                                                                                      cGCTGCCTTCCAGTGCACCAGGCGCTAGGGGCGTTGGTGCGCGGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTggg                                                                             <  2:515482/249‑1 (MQ=255)                                                                                                                                                                                        cgggggcTAAGGGGTTTGGTGCGCCGGGGAACCCGCTCCGGGTTCCCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCCCCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGAt                                                          <  2:169804/245‑1 (MQ=255)                                                                                                                                                                                         aGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATa                                                         <  1:658004/250‑1 (MQ=255)                                                                                                                                                                                           gcgcTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATAc                                                        >  1:674313/1‑249 (MQ=255)                                                                                                                                                                                            cgcTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACg                                                       >  1:116984/1‑249 (MQ=255)                                                                                                                                                                                              cTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAAc                                                    >  1:300806/1‑250 (MQ=255)                                                                                                                                                                                                aaGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCTGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACACTCATCGGATCGTTACGAACtt                                                  >  2:241117/1‑250 (MQ=255)                                                                                                                                                                                                aaGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACtt                                                  >  2:788080/1‑250 (MQ=255)                                                                                                                                                                                                         ggTGCGCCGAGAAAACCGCTCCTGGTTCCCGGCGGGCCGGTCGGGGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCCCCCGGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGTTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGtgttg                                          <  2:576398/249‑1 (MQ=255)                                                                                                                                                                                                          gTGCGCAGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTgttgtt                                        <  1:256244/250‑1 (MQ=255)                                                                                                                                                                                                                 gAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACgtg                                 >  2:28676/1‑250 (MQ=255)                                                                                                                                                                                                                 gAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACgtg                                 >  1:444278/1‑250 (MQ=255)                                                                                                                                                                                                                    gAAACCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCCCCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTa                              <  1:673147/250‑1 (MQ=255)                                                                                                                                                                                                                     aaaCCGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTaa                             <  1:392615/250‑1 (MQ=255)                                                                                                                                                                                                                         cGCTACTGGTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACg                         <  1:629664/250‑1 (MQ=255)                                                                                                                                                                                                                               gggTTCCCGGCCCGCCGGGCGGGGTTTCGTTTTTTGGCTGCGAATTCCTGACCGGAAGAAGGCCACCCGGGGGGCCGGACACTGAACTTGTTCCGGCGCCACCGCTTAAATCGGGGGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACGGTCAg                    <  2:682283/248‑1 (MQ=255)                                                                                                                                                                                                                                 gTTACCGGCGCGCCGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACGGTCAGCTg                 >  1:422721/1‑250 (MQ=255)                                                                                                                                                                                                                                              cGGTCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACGGTCAGCTGCCGCGCGAGAAAg    >  2:641306/1‑250 (MQ=255)                                                                                                                                                                                                                                                 tCGGAGCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGCACTTTGGTGTTGTTTGACGTGTTAAGACGGTCAGCTGCCGCGCGAGACAGTg  >  1:593425/1‑249 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    GCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACGGTCAGCTGCCGCGCGAGAAAGTG  >  NZ_CP009273/659719‑660207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑