Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,089,5710AG35.3% 117.9 / 18.3 68I83V (ATT→GTT) hisFimidazole glycerol phosphate synthase subunit HisF
Reads supporting (aligned to +/- strand):  ref base A (9/35);  new base G (24/0);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 2.56e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CATGCTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAG  >  NZ_CP009273/2089324‑2089820
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         
cATGCTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGtt                                                                                                                                                                                                                                                         >  2:558812/1‑250 (MQ=255)
  tGCTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGCGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAACAGCTGGGTATCTCGCGTGGCGGAAGAGCTCGACATTCCGTTTAGTGTGGCGGGTGGGtttga                                                                                                                                                                                                                                                       >  1:937217/1‑248 (MQ=255)
       gCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGtctc                                                                                                                                                                                                                                                  <  2:773276/250‑1 (MQ=255)
        cAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAggtttt                                                                                                                                                                                                                                                 >  1:36030/1‑245 (MQ=255)
         aaaaCGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGAGGAACTGGTGTTCTACGAAATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCGGTTTTGGGGTGCGGGGGGGAGTAAGtgttgg                                                                                                                                                                                                                                                >  2:461853/1‑245 (MQ=255)
            aCGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTGCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGaa                                                                                                                                                                                                                                             <  1:228565/250‑1 (MQ=255)
                aTAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAGTGTCCGGGCGATg                                                                                                                                                                                                                                         >  2:85066/1‑250 (MQ=255)
                   aTCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAAGCTCTGGACGAttgcg                                                                                                                                                                                                                                      >  2:846159/1‑246 (MQ=255)
                    tCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCcgc                                                                                                                                                                                                                                     <  1:131226/250‑1 (MQ=255)
                      ccATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCcgcg                                                                                                                                                                                                                                    >  1:389146/1‑249 (MQ=255)
                         tGTCGCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGGGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGaaaa                                                                                                                                                                                                                                <  2:503885/250‑1 (MQ=255)
                           tctcGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAAtt                                                                                                                                                                                                                              <  2:471919/250‑1 (MQ=255)
                              cGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTTGAAGATGCCGCGAAAATTCtt                                                                                                                                                                                                                           >  1:518805/1‑250 (MQ=255)
                               gACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTc                                                                                                                                                                                                                             <  2:161294/247‑1 (MQ=255)
                                aCGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAGTCTCTGGAAGGTGCCGCGAAAATTCtttt                                                                                                                                                                                                                         >  1:154508/1‑249 (MQ=255)
                                 cGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTGTCTGGGAAATGCCGGGAAAAATCttttc                                                                                                                                                                                                                        >  1:861533/1‑248 (MQ=255)
                                  gTTCGTGATTGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCt                                                                                                                                                                                                                       <  1:860338/250‑1 (MQ=255)
                                          tGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGcgcg                                                                                                                                                                                                               <  2:252941/250‑1 (MQ=255)
                                           ggTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGGCTCTGGGAGATGGCGCGAAAATTATTTTCTTTGGCGCgg                                                                                                                                                                                                              >  1:715034/1‑250 (MQ=255)
                                            gTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCgg                                                                                                                                                                                                              <  1:232519/249‑1 (MQ=255)
                                               aGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATaa                                                                                                                                                                                                          <  1:361832/250‑1 (MQ=255)
                                                 gtggtgAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGCTCTGGAAGATGCCGCGGAAATTTTTTCCTTTTGGGCGGGAaaaa                                                                                                                                                                                                        >  1:262017/1‑250 (MQ=255)
                                                            cGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAAc                                                                                                                                                                                             <  1:740988/250‑1 (MQ=255)
                                                             gTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTTAATCTCTTGGAGATGGCCGGCAAAATCTTTCCTTTTGGGCGGGTAAAATTTCCaataaca                                                                                                                                                                                            >  1:131131/1‑244 (MQ=255)
                                                                cAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTTGGAGATGCCGCGGAAATTCTTTTCTTTTGGGCGGGGAAAATTTCTATCAACtctc                                                                                                                                                                                         >  2:295301/1‑250 (MQ=255)
                                                                cAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAActct                                                                                                                                                                                          <  1:773297/249‑1 (MQ=255)
                                                                         aaCCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTgg                                                                                                                                                                                <  1:765755/250‑1 (MQ=255)
                                                                             aTGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGa                                                                                                                                                                            <  1:657463/250‑1 (MQ=255)
                                                                                         tcGATATCGTGCCGCTGGCAAAACGCTACGCATAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACattaat                                                                                                                                                                <  2:97237/249‑1 (MQ=255)
                                                                                                    ccGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTgg                                                                                                                                                     <  2:158950/250‑1 (MQ=255)
                                                                                                      gCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTTGAAGATGCCGCGGAAATTCTTTTCTTTTGCGCGGGTAAAATTTTCATCAACTCTCCTGCGCTGGCAGACCCAACATTTATTAATCGCCTGGcc                                                                                                                                                   >  1:892489/1‑250 (MQ=255)
                                                                                                          gCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGa                                                                                                                                                 <  1:202256/248‑1 (MQ=255)
                                                                                                          gCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGa                                                                                                                                                 <  1:212376/248‑1 (MQ=255)
                                                                                                           cAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGCCCCGAAAAATTTTTTCTTTTGCGCGGGTAAAATTTCCATCAACTCTCCTTCGCTGGGCGACCCAACATTAATTTCTCGCCTGGCCGATCg                                                                                                                                              >  1:109347/1‑250 (MQ=255)
                                                                                                                     cgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGt                                                                                                                                     <  1:823852/249‑1 (MQ=255)
                                                                                                                     cgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTTCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCCGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTg                                                                                                                                    >  1:250607/1‑250 (MQ=255)
                                                                                                                                gcgcTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATtgt                                                                                                                         <  2:89778/250‑1 (MQ=255)
                                                                                                                                   cTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGt                                                                                                                      <  2:661263/250‑1 (MQ=255)
                                                                                                                                       cGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCGGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGt                                                                                                                  <  1:691704/250‑1 (MQ=255)
                                                                                                                                                       gATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTacg                                                                                                  <  1:727045/250‑1 (MQ=255)
                                                                                                                                                         tatCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGGAGATGCCGCGGAAAATCTTTTCTTTTGCGCGGGTAAAATTTTCCTCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTTGTacgac                                                                                                >  1:507907/1‑250 (MQ=255)
                                                                                                                                                                gCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGaaa                                                                                         <  2:752025/250‑1 (MQ=255)
                                                                                                                                                                 cTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGGCTCTGGAAGATGCCGCGAAAAATTTTTTCTTTGGGGCGGGGAAAATTTTCCTCAACTCTCCTGCGCTGGCAGAACCCACATTAATTACTCCCCTGGCCCATCCCTTTGGCGGGCAGAGTATTTTGGTGGGGATTGATACCCGGGACGACGCCGAAAc                                                                                        >  2:127046/1‑250 (MQ=255)
                                                                                                                                                                    ccAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAAcc                                                                                       <  1:895967/248‑1 (MQ=255)
                                                                                                                                                                     cAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCgg                                                                                     <  1:804112/249‑1 (MQ=255)
                                                                                                                                                                       gCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGCCGCGGAAATTCTTTCCTTTGGCGCGGGTAAAATTTTCATCAAATCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCCGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGGATTGATACCTGGTACGACGCCGAAACCggga                                                                                   >  1:477826/1‑247 (MQ=255)
                                                                                                                                                                           tGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGCTCTGGGAGAAGTCGCGAAAATTCTTTCCCTTGGGGCGGGTAAAATTTCCATCAACTCTCCTGCGCTGGCAGAACCCACATTTATTATTCGCCTGGCCGATCGCTTTGGGGTGCCGGGTGATGTGGTGCGGATTTATACCTGGTGTGACGCCCGAACCgtgcaaatc                                                                              >  2:357229/1‑242 (MQ=255)
                                                                                                                                                                           tGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGGTTCTGGAAGATGTCGCGCAAATTCTTTCCTTTGGGGCGGGTAAAATTTCCATCAACTCTCCTGCTCCGGCAGACCCAACATTAATTATTTGCCCGGCCGGTCGCTTTGGTGTGCTGTGCAATGTGGTCGGTATTGACACCTGGTGCGACGCCGCAACCCGCAActat                                                                              >  2:464288/1‑250 (MQ=255)
                                                                                                                                                                             gCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGGAGATGCCGCGAAAATTCTTTCCTTTTGCGCGGGTAAAATTTTCATCAACTCTCCCCCGCTGGCAGAACCCACATTAATTACTCGCCTGGCCGATCGCCTTTGTGTGCGGTGTATTGTGGGTGGGATTGATACCTGGTACGGCGCCGGAACCGGTAAATATCa                                                                            >  2:537958/1‑250 (MQ=255)
                                                                                                                                                                                 tgtgGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTTTGGCTCTGGGAGATGCCGCCGAAAATCTTTTCCTTGGCGCGGGTAAAATTTCCATCAAATCCCCCGCGCTGGCCGACCCCACATTAATTAATCGGCTGGCCGACCCCTTTCGCGCGCCGTGGTTTGTGGGCGGCCGTGATTCCTGGTACGAAGCCGAGAACGGTGAATAACAtggg                                                                        >  2:253569/1‑248 (MQ=255)
                                                                                                                                                                                      tAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGTTCTGGGAGATGGCGCGGAAAATCTTTTCTTTGGCGCGGATAAAATTTCCATTAACTCTCCTGCGCTGGCAGACCCAACCTTAATTAATCGCCTTGCCGGTCGCTTTGGCGTGGCGTGTATTGTGGGCGGGAATTATAACTGGGAGGACACAGAAACCCGGAAATATAACGTGAAta                                                                    >  2:588826/1‑248 (MQ=255)
                                                                                                                                                                                       aGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGGCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTTGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTTGCGTGCAGTGTATTTTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGGAAATATCATGTGAATCaa                                                                  >  1:686380/1‑250 (MQ=255)
                                                                                                                                                                                          tAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAatat                                                               <  1:172700/250‑1 (MQ=255)
                                                                                                                                                                                          tAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGGAGAAGCCGCGAAAAATTTTTTCTTTTGCGCGGGGAAAAATTTCATCAACTCTCCTGCGCTTGCAGAACCAAAAATAATTAATTGCCTCGCCGATCGCTTTGGCGTGCAGTGTAATGTGGTGGGGATTGATTACCGGTAAGACGCCGAAAACGGTAAATATCATGTGGATCAata                                                                >  2:66168/1‑249 (MQ=255)
                                                                                                                                                                                           aaaaGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTTAGTCTTTGGTAGATGCCCCGGAAAATCTTTCCTTTTGCGCGGGTAAAATTTTCATCAACTCCCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCCTTTGCGTGCAGGGTATTTTGGTCGGTATTTATTCCTGTGACGACGCCCGAACAGGGTAATATCATGTGAATCAAtata                                                              >  2:421350/1‑250 (MQ=255)
                                                                                                                                                                                              aGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCg                                                           <  1:770093/250‑1 (MQ=255)
                                                                                                                                                                                                                 cGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCcg                                        <  2:389146/250‑1 (MQ=255)
                                                                                                                                                                                                                    aaGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGt                                     <  2:89349/250‑1 (MQ=255)
                                                                                                                                                                                                                     aGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTc                                    <  1:28940/250‑1 (MQ=255)
                                                                                                                                                                                                                        gATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCCCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTTTGGTCGGGATTGATACCTGGTACGACGCCGAAACCGGGAAATATCATGTGAATCAATATAACGGCGATGAAAGCCGCACCCCCGTCACt                                 >  1:482269/1‑250 (MQ=255)
                                                                                                                                                                                                                                  ccGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGAAGCCGCGGAAAATTTTTCCTTTGGCGCGGATAAAATTTCCCTCAACTCTCCTGCGCTGGCAGACCCAACAATAAATAATCGCCTGGCCGAACGCTTTGGCGTGCAGGGTATTGTGGGCGGGATTTATACCTGGTACCACCCCGAAACCGGTAAATATCATGTGAATCAATATAACGGCCCAGAAAACCACCCCCCCGTCCCTCacagggcg                        >  2:937019/1‑242 (MQ=255)
                                                                                                                                                                                                                                       ttGTGTGGCGGGGGGGGGTAAGTCCCTGGGAGATGCCGCGAAAATTTTTTTCTTTTGCGCGGGTAAAATTTTCATCAAATCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGGGGATTGTGGTCGGTATTGATAACTGGTACGACGCCGAAACCGGGAAAAATACTGTGAATCAATATACCGGCGATGAAAGCCGCACCCCCGGCACCCAATGGGAAACGccc                  >  2:591987/1‑248 (MQ=255)
                                                                                                                                                                                                                                         gtgtgGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCg                 <  2:36030/249‑1 (MQ=255)
                                                                                                                                                                                                                                            tgGCGGGTGGGATTAAGTCCCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTg             >  1:664037/1‑250 (MQ=255)
                                                                                                                                                                                                                                             ggCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTgg            <  2:26239/250‑1 (MQ=255)
                                                                                                                                                                                                                                                  gTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTa         >  2:716581/1‑248 (MQ=255)
                                                                                                                                                                                                                                                  gTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTa         >  2:272583/1‑248 (MQ=255)
                                                                                                                                                                                                                                                     ggATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAgg     <  1:909337/249‑1 (MQ=255)
                                                                                                                                                                                                                                                     ggATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAgg     <  1:571703/249‑1 (MQ=255)
                                                                                                                                                                                                                                                       aTTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAg  >  1:509935/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         
CATGCTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAG  >  NZ_CP009273/2089324‑2089820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑