Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,221,729	0	A	T	35.1%	73.7 / 17.7	58	pseudogene (509/1436 nt)	mdtQ	multidrug resistance outer membrane protein MdtQ
Reads supporting (aligned to +/- strand):  ref base A (14/23);  new base T (20/0);  total (34/24)
Fisher's exact test for biased strand distribution p-value = 1.67e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.05e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TCCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑GTAACCT‑CCGCCCGA‑TTC‑TTTCCCC‑AGATA‑TCGAGA‑TGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAA  >  NZ_CP009273/2221482‑2221976                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                      tcCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTaa                                                                                                                                                                                                                                                                      <  1:527561/249‑1 (MQ=255)    ccGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTT‑CTCGC‑C‑TT‑CCCCCGCCCC‑TG‑CCTTTA‑ccc                                                                                                                                                                                                                                                                  >  1:15130/1‑248 (MQ=255)    ccGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGAACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTAA‑CCGt                                                                                                                                                                                                                                                                 <  2:520665/250‑1 (MQ=255)          cATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑TT‑TCCCCGCCCG‑TG‑CTTTTA‑CCGTAccccg                                                                                                                                                                                                                                                           >  1:844971/1‑248 (MQ=255)           aTCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTT‑TTCTCGG‑TT‑CCG‑CGCCCC‑CG‑CTTTTA‑CCCGACCCCgg                                                                                                                                                                                                                                                          >  2:794557/1‑250 (MQ=255)            tCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTACCCAGGc                                                                                                                                                                                                                                                         <  2:777740/250‑1 (MQ=255)                tGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTG‑CTCGC‑G‑TT‑TCGCCCCCCC‑TG‑CCTTTA‑ACGTACACAAGC‑GGGc                                                                                                                                                                                                                                                    >  1:931799/1‑250 (MQ=255)                  aGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑g                                                                                                                                                                                                                                                 <  1:1075/250‑1 (MQ=255)                    ctgctgCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTTGC‑CGGTTTT‑GTCGC‑G‑TTCCCCCCGCCCT‑GG‑TTTTAC‑CCGCTCCCAGCG‑GGGcgt                                                                                                                                                                                                                                                  >  2:667587/1‑247 (MQ=255)                          ccGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTC‑TTCCC‑G‑TT‑‑CGCCCCCCC‑TG‑CCTTTA‑CCGTTCCCCGGC‑GGGCC‑GGAACCT‑cc                                                                                                                                                                                                                                        >  2:340873/1‑250 (MQ=255)                              gTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTG‑CTCGC‑G‑GT‑TCGCCGCCCC‑GG‑CTTTTA‑CCGGACCCAGGC‑GGGGC‑GTAACCT‑CCGccc                                                                                                                                                                                                                                    >  1:284359/1‑250 (MQ=255)                                   gCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑TT‑CCGCCGCCCC‑TG‑CTTTTA‑CCGTACCCCGGC‑GGGGA‑GTAACcc‑cccccccgttt                                                                                                                                                                                                                               >  1:740693/1‑245 (MQ=255)                                       gCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑GTAACCT‑CCGCCCGA‑TTC‑TTTc                                                                                                                                                                                                                         <  2:364873/250‑1 (MQ=255)                                          ttAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑GTAACCT‑CCGCCCGA‑TTC‑TTTcccc                                                                                                                                                                                                                      <  1:393094/250‑1 (MQ=255)                                          ttAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑GTAACCT‑CCGCCCGA‑TTC‑TTTcccc                                                                                                                                                                                                                      <  1:417877/250‑1 (MQ=255)                                            aaTATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑GTAACCT‑CCGCCCGA‑TTC‑TTTCCCC‑a                                                                                                                                                                                                                    <  1:218451/249‑1 (MQ=255)                                            aaTATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTG‑CTCGC‑G‑GT‑GCGCCGCCCC‑TG‑CTTTTA‑CCGTAACCCGGC‑GGGGA‑GTAGACT‑CCGCCCCA‑TTC‑TTTtccccc                                                                                                                                                                                                                    >  2:5710/1‑248 (MQ=255)                                                    tttCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTG‑CTCGC‑G‑TT‑TCGCCGCCCG‑TG‑CTTTTA‑CCCTACCCAGGG‑GGGGA‑GGAAACC‑CCGCCCGC‑GTC‑TTTTCCC‑CGATA‑Tc                                                                                                                                                                                                             >  1:554723/1‑247 (MQ=255)                                                      tCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTT‑CTCGC‑G‑GT‑CCCCCCCCCG‑TG‑CTTTTA‑ACGCACCCCGGC‑GGGCA‑GGAACCC‑CCGCCCCA‑TTC‑TTTTCCC‑AGATA‑TCGAGA‑t                                                                                                                                                                                                       >  1:925999/1‑250 (MQ=255)                                                        cacaCCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑GTAACCT‑CCGCCCGA‑TTC‑TTTCCCC‑AGATA‑TCGAGA‑Tg                                                                                                                                                                                                      <  1:144538/249‑1 (MQ=255)                                                           acCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTTA‑CCGTACCCCGGC‑GGGCA‑GTAACCT‑CCGCCCCA‑TTC‑TTTTCCC‑CGATA‑TCGGGA‑TGCC‑aa                                                                                                                                                                                                 >  1:379569/1‑249 (MQ=255)                                                           acCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑GTAACCT‑CCGCCCGA‑TTC‑TTTCCCC‑AGATA‑TCGAGA‑TGCC‑Ag                                                                                                                                                                                                 <  2:245973/250‑1 (MQ=255)                                                             cTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑GTAACCT‑CTGCCCGA‑TTC‑TTTCCCC‑AGATA‑TCGAGA‑TGCC‑AGcc                                                                                                                                                                                               <  1:759253/250‑1 (MQ=255)                                                               tCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑GG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑GTAACCT‑CCGCCCGA‑TTC‑TTTCCCC‑CGATA‑TCGAGA‑TGCC‑AGCccg                                                                                                                                                                                             >  1:950825/1‑250 (MQ=255)                                                                        gaagTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTT‑CTCGC‑G‑GT‑CCCCCCCCCGCGG‑CTTTAA‑CC‑TACCCAGGCGGGGGG‑GTAACCC‑CCCCCCCG‑TTT‑TTTCCCC‑‑GATA‑TCCAGA‑TTGC‑CGCCCC‑CCCTTTAG‑c                                                                                                                                                                                  >  2:186939/1‑250 (MQ=255)                                                                             aaTCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTT‑CTCGC‑G‑GT‑CCGCCGCCCG‑TG‑CTTTTA‑ACGGAACCAGGG‑GGGCA‑GGAAACT‑CCCCCCCA‑TTT‑TTTTCCC‑CAATA‑TCGGGA‑TGCC‑CGCCCGCCCGTAAAG‑CCaa                                                                                                                                                                               >  1:656023/1‑249 (MQ=255)                                                                              aTCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTT‑CTCGC‑G‑TT‑CCCCCCCCCC‑CG‑CTTTTA‑CCCTACCCACGC‑GGGGC‑GGAACCC‑CCCCCCCA‑TTC‑TTTTCCC‑CGCTA‑TCCCGC‑TGCC‑CACCCC‑CCCTTAAG‑CCacaa                                                                                                                                                                             >  2:678743/1‑249 (MQ=255)                                                                                      cTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑GT‑CCGCCGCCCG‑TG‑CTTTTA‑CCCTACCCCGGC‑GGGGC‑GTAAACT‑CCGCCCCA‑TTC‑TTTCCCC‑CCATA‑TCGCGA‑TGCC‑AGCCCC‑CCGTTAAA‑CCAAAAGTA‑CCCttt                                                                                                                                                                   >  1:855003/1‑249 (MQ=255)                                                                                       tGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑TT‑CCCCCGCCCG‑TG‑CTTTTA‑CCGTACCCAGGC‑GGGCA‑GTAACCC‑CCGCCCCA‑TTC‑TTTCCCC‑ACATA‑TCGAGA‑AGCC‑AGCCCC‑CCGTTAAA‑CCAAAAGTA‑cccctcg                                                                                                                                                                  >  1:354018/1‑245 (MQ=255)                                                                                         atatAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTT‑CTCGC‑G‑TT‑CCCCCCCCCC‑TG‑CTTTTA‑CCCGACCCCGGC‑GGGGC‑GTAACCT‑CCCCCCGC‑TTC‑TTTTCCC‑CGATA‑TCGAGA‑TGCC‑AACCCC‑CCCTTAAA‑CCAAAAAGA‑CCCTTtgtg                                                                                                                                                                >  1:504990/1‑250 (MQ=255)                                                                                               cTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑GTTT‑CCCCCCCCCC‑GG‑CTTTAC‑CCGACCCCAGGC‑GGGC‑‑GGCACCT‑CCCCCCCACTTC‑TTTCCCC‑A‑ATATTCGAAA‑GGCC‑CCCCCG‑CCCTTATG‑CCAAAAGAA‑CCTTTggggaccac                                                                                                                                                           >  2:356348/1‑243 (MQ=255)                                                                                               cTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑GT‑TCGCCCCCCG‑TG‑CTTTTA‑CCGTACCCAGGC‑GGGCC‑GGAAACT‑CCCCCCGG‑TTT‑TTTCCCC‑AGATA‑TCTAGA‑TGCC‑AGCCCC‑CCCTTTAG‑CCCAAAATA‑CCCTTTGGGGACCA‑c                                                                                                                                                         >  1:256083/1‑250 (MQ=255)                                                                                                         tgcgcgtgagtGGTGTTCTTCTTTTTTTCTGTGTG‑CTGCAAGCCCGTGTTTCA‑CGCCGCCTGGTTTGCCCCCTCCCAG‑ACAGGGCGGGCTCCGCGGCCAGCCAGCAAGGGGG‑GGTTTTC‑CCCGC‑G‑TC‑CCGCCGCCCG‑TC‑CTTTAC‑CCGTCCCCAGGG‑GGGCA‑GAACCCT‑CCGCCCGA‑TTC‑TTCCCCC‑AGATA‑TCGAGA‑TGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCg                                                                                                                                               <  2:643124/240‑1 (MQ=255)                                                                                                           cgcgATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTT‑CTCCC‑G‑GT‑CCGCCGCCCG‑CG‑CTTTAA‑ACGCACCCCAGC‑GGGGAGGCAACCC‑CGCCCCCA‑TCC‑TTTCCCC‑ACA‑A‑TCCAGA‑AGGC‑CGCCCC‑CCCTTTAGACCAAAATAA‑CCCTTGGGGTCCAC‑CGGGCCggcgga                                                                                                                                              >  2:104095/1‑246 (MQ=255)                                                                                                                  aTGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCCGCAATTGGC‑GGGGTTT‑GTCGC‑G‑GT‑TCGCCGCCCC‑GT‑CTTTTA‑CACTACCCAGGG‑GGGGC‑GGAACCTACCCCCCCA‑TTT‑TTTCCCC‑CCATA‑‑CGAGA‑TGCCAAGCCCG‑CCG‑TAAG‑CCCAAAGTATCCTTTGGGTTCCCA‑CGGGCCgtcgtctctcggg                                                                                                                                       >  2:304275/1‑238 (MQ=255)                                                                                                                      ttgttttgttttttttTATTTGCCTGAAAGCCGGTGTTTAC‑CGCCGCTGGGGTTTCCCCCCCCCAGTACG‑GGGGGCCTCCGTTGCCGGCCAGAAATGGGC‑GGTTTTC‑CCCGC‑T‑TC‑CCGCCGCCGG‑TG‑TTTTAC‑CCGTCCCCGGGG‑GGGCA‑GTACCCC‑CCGCCCGA‑TTC‑TTCCCCC‑AGATA‑TCGAGA‑GGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCg                                                                                                                                   <  2:758043/240‑1 (MQ=255)                                                                                                                        ttCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTACCCCGGC‑GGGCA‑GGAACCC‑CCGCCCGA‑TTC‑TTTCCCC‑AGATA‑TTGAGA‑TGCC‑AGCCCC‑CCGGTAAG‑CCCAAAGTA‑CCGTTGGTGTAACA‑CCGGCCGGTCGTACCTGCGGCCgggt                                                                                                                                >  1:755269/1‑248 (MQ=255)                                                                                                                            gCTGTTTTTCTATTTG‑CGCCAGGACC‑TGATTAACCGCGGCTTGGTTTGGCCACCCCCAGGACA‑GGCGGGCTGCGCGGCCAGCCACCATGGGGG‑GGGTTGG‑CCGCC‑G‑TC‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTCCCCAGGG‑GGGCA‑GTACCCT‑CCGCCCGA‑TTC‑TTTCCCC‑AGATA‑GCGAGA‑TGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGtt                                                                                                                            <  2:199241/250‑1 (MQ=255)                                                                                                                                tttttCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑GTCGC‑G‑GT‑GCGCCCCCCG‑CG‑CCTTTA‑ACGGACACCGGC‑GGGCT‑GTAACCT‑CCTCCCGC‑TTCTTTTCCCC‑CGA‑A‑TCGAGC‑AGAC‑CGCCCC‑CCCGTAAG‑CCCCAAAAA‑CCGTTTGGGTAGCA‑CCGGGCGCGCGCACCCCCGGcccggacggttg                                                                                                                          >  2:725510/1‑238 (MQ=255)                                                                                                                                   ttCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTT‑CTCGC‑G‑GT‑CCGCCCCCCG‑TGCCTTTAC‑CCGCCCCAAGCG‑GGGCG‑GAAACTC‑CCCCCCCT‑TTT‑TTTCCCC‑AG‑TA‑TCCGGATTGCC‑ACCCCC‑CCGTTAGC‑CCAAAAGAC‑CCTTTGGGTTCCCA‑CGGGCCGGGTGTCCCT‑CGGCCgggcacgtgcaagg                                                                                                                      >  2:411930/1‑237 (MQ=255)                                                                                                                                                 aaGCCCGTTTTTAA‑CGCCGCCTGGGTTTCCCCCCCCCAGTACA‑GGCGGGCTCCGCTGCCAGCCAGCAATTGGG‑GGGTTTG‑CTCGC‑G‑TC‑CCGCCCCCCG‑TG‑CTTTAA‑CCGTCCCCAGGC‑GGGCA‑GTACCCT‑CCGCCCGA‑TTC‑TTTCCCC‑AGATA‑TCGAGA‑TGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTa                                                                                                        <  1:78903/250‑1 (MQ=255)                                                                                                                                                                          ttgccccccccccAGTACA‑GGGGGGCTACCTCGCCGCCCAGAAGTTGG‑‑GGGTGTGCCCCCC‑C‑TC‑CCCCCCCCCG‑TT‑CTTTAC‑CCGTCCCGGGGG‑GGGAA‑AAACCCC‑CCCCCCTA‑TTT‑TTCCCCA‑AGATA‑CCGAGA‑GGCC‑GGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGcc                                                                                <  2:179261/240‑1 (MQ=255)                                                                                                                                                                            ttcccccTCCCAGAACG‑GGCGGGCTCCGCTGCCAGCCAGCAATTGGC‑GGGTTGG‑CCCGC‑G‑TC‑CCGCCGCCCG‑TG‑CTTTAC‑CCGTCCCCAGGC‑GGGCA‑GTACCCT‑CCGCCCGA‑TTC‑TTTCCCC‑AGATA‑TCGAGA‑TGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCg                                                                               <  1:549731/243‑1 (MQ=255)                                                                                                                                                                                 acccccAGTACA‑GGCGGGCTACGCTGCCAGCCAGAAAGGGGC‑GGTTTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑GAAACCT‑CCGCCCGA‑TTC‑TTTCCCC‑AGATA‑TCGAGA‑TGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCat                                                                         <  1:144120/247‑1 (MQ=255)                                                                                                                                                                                       aGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTT‑CTCGC‑G‑GT‑TCGCCGCCCC‑TG‑GTTTTA‑CCCTAACCAGGC‑GGGGG‑GTAACCT‑CCCCCCCG‑TTC‑TTTTCCC‑CCCTA‑TTTAGG‑TGGC‑CGCCCC‑CCCTTTAGCCCAAAAGAA‑CC‑CTGGGGGACCACCGGGCGGGCCGCACCGCGGGCGGGACCGTTC‑GGGGAAACGGGCCCACTTAGGCCTCTGCCGCCCTTTTTTTCCCCCCCCTATcccc                                                                   >  2:642416/1‑248 (MQ=255)                                                                                                                                                                                          gacgggCGGGTCACGCTCCCACCCAGCATTGGGG‑GGGTTCG‑CGCGC‑C‑TC‑CCGCCCCCCT‑TG‑CTTACC‑CCGTCCCCGGGC‑GGGCA‑GTCCCCC‑CCCCCCTT‑TTC‑TTTCCCC‑AGATA‑TCGAGA‑TGCC‑CCCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATccgccg                                                                 <  2:478438/246‑1 (MQ=255)                                                                                                                                                                                             gggCGGGCTACGCTGCCAGCCGGCAATTGGC‑GGTTTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TC‑CTTTAC‑CCGTCCCCAGGC‑GGGCA‑GTACCCC‑CCGCCCGA‑TTC‑TTCCCCC‑AGATA‑TCGAGA‑TGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGaaa                                                              <  1:81966/249‑1 (MQ=255)                                                                                                                                                                                                     tACGCTGCCAGCCAGCAATTGGC‑GGGGTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑GAAAACT‑CCGCCCGA‑TTC‑TTTCCCC‑CGATA‑TCGAGA‑AGCC‑AGCCCG‑CCGGTAAG‑CCAAAAATA‑ACGTTGGTGTACCA‑CGGGCCGGGCGTACCTTCGGCCGGCACGGTCCGAGCAAACGGCCCCCTTAAGCCTTCTTCCGACCCTTTTTTCCGCGCCCTCTCCCCCGTAAAGTCGCTc                                                      >  2:636891/1‑250 (MQ=255)                                                                                                                                                                                                     cccgcgcccacccacCAATGGGC‑GGTTTGC‑CTGGC‑C‑TT‑CCGCCCCCGC‑TG‑CTCTAC‑CCGTCCCCAGGT‑GGGCA‑TCACCCT‑CCGCCCGA‑TTC‑TTCCCCC‑AGATA‑TCGAGC‑TGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATc                                                      <  2:589000/243‑1 (MQ=255)                                                                                                                                                                                                                        tGGC‑GGTTTTG‑CCCGC‑G‑TC‑CCGCCGCCCG‑TG‑CTTTAC‑CCGACCCCAGGC‑GGGCA‑GTAACCC‑CCGCCCGT‑TTT‑TTCCCCC‑AGATA‑TCGAGA‑TGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTg                                    <  1:36408/249‑1 (MQ=255)                                                                                                                                                                                                                           c‑GGTTTGG‑CGCGC‑G‑TC‑CCGCCCCCCG‑TG‑CTTTAC‑CCGTACCCAGGC‑GGTCA‑GTACCCT‑CCGCCCGA‑TTC‑TTCCCCA‑AGATA‑TCGAGA‑TGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCaa                                <  1:525121/250‑1 (MQ=255)                                                                                                                                                                                                                                   g‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑GTAACCT‑CCGCCCGA‑TTC‑TTTCCCC‑AGATA‑TCGAGA‑TGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGCCGATCTGCGGCCCATCCTGAGTCGCAACCGCCtt                         >  2:315655/1‑250 (MQ=255)                                                                                                                                                                                                                                              t‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑GTAACCT‑CCGCCCGA‑TTC‑TTTCCCC‑AGATA‑TCGAGA‑TGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGGCCCATTAAGCCTTATTCCGACATTTTTTGCCGCCCCATATCCGCCGACAAGTCGATCTGCGTAGCATCCTGAGGGGCAACCGCCTTCGCCTggg                 >  2:535790/1‑250 (MQ=255)                                                                                                                                                                                                                                              t‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑GTAACCT‑CCGCCCGA‑TTC‑TTTCCCC‑AGATA‑TCGAGA‑TGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTggg                 >  1:758850/1‑250 (MQ=255)                                                                                                                                                                                                                                                 cgccgcCCG‑TG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑GTAACCT‑CCGCCCGA‑TTC‑TTTCCCC‑AGATA‑TCGAGA‑TGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCt               >  1:586911/1‑250 (MQ=255)                                                                                                                                                                                                                                                 cgccgcCCG‑TG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑GTAACCT‑CCGCCCGA‑TTC‑TTTCCCC‑AGATA‑TCGAGA‑TGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGAGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCCAACGGCCCCATTAAGCCTTCTGCCGACCTTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCt               >  2:893526/1‑250 (MQ=255)                                                                                                                                                                                                                                                       ccccggtcTTTAACCCGCACCCGGGC‑GGGCA‑GCAACCC‑CCCCCCG‑‑TTC‑TTTCCCCAAGTAA‑CCGGGA‑TGCC‑GGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCa            <  2:433544/243‑1 (MQ=255)                                                                                                                                                                                                                                                       ccG‑TG‑CTTTAA‑CCGTCCCCGGGG‑GGGCA‑TTACCCC‑CCGCCCGA‑TTC‑TTCCCCC‑AGATA‑TCGAGA‑GCCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCagct         <  1:127918/250‑1 (MQ=255)                                                                                                                                                                                                                                                                ttAA‑CCGTACCCAGGC‑GGGCA‑GTAACCT‑CCGCCCGA‑TTC‑TTTCCCC‑AGATA‑TCGAGA‑TGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGaa  >  1:459223/1‑250 (MQ=255)                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                      TCCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTG‑CTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTGCCACTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTG‑CTCGC‑G‑TT‑CCGCCGCCCG‑TG‑CTTTAA‑CCGTACCCAGGC‑GGGCA‑GTAACCT‑CCGCCCGA‑TTC‑TTTCCCC‑AGATA‑TCGAGA‑TGCC‑AGCCCG‑CCGTTAAG‑CCAAAAGTA‑CCGTTGGTGTACCA‑CGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAA  >  NZ_CP009273/2221482‑2221976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑