| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,845,013 | 0 | T | G | 32.9% | 152.8 / 17.4 | 73 | G218G (GGT→GGG) | hypB | hydrogenase nickel incorporation protein HypB |
| Reads supporting (aligned to +/- strand): ref base T (6/43); new base G (24/0); total (30/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.01e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGTTATTGAAGGCGACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATT > NZ_CP009273/2844764‑2845258 | aGTTATTGAAGGCGACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGt < 1:452775/250‑1 (MQ=255) tGAAGGCGACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTTACCAAGGTGAAGa > 1:438347/1‑249 (MQ=255) cGACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGTTTCCGAAGGGGAAGACAAACCa > 1:952913/1‑250 (MQ=255) gACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGGGGCGGTGC‑TTTCCGTTTCCGCAGGGTGAGACAAAcccc > 2:714044/1‑248 (MQ=255) gaGCACACCGTGAATGGGGCCGCGCGCATTCCCGCTACCGGCACACCAGCGATTCAGGTGAACTCCGGTAAAGGCTGCCATCTTGACGCACAGAGGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACGTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACtta < 2:914333/249‑3 (MQ=255) aaaCCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAACGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAata < 2:492701/250‑1 (MQ=255) aaaCCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGT‑TTTCCGTTTACGAAGGTGGAGACAAACCACTGaaaa > 2:739021/1‑248 (MQ=255) aaaCCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGGTTACGGAGGGGAAGACAAAACACTGaaaca > 1:672103/1‑248 (MQ=255) cGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTTCCGCAGGGGAAGACAAACCACTGAAAAATCCGCa‑aata > 1:119509/1‑249 (MQ=255) gATGCCGCCCGCATTCGCGATACCGACGCCTCAGCTACTCAGGTGACCACCGGTAAAGGCTGACATCTTGACACACAGCTCATTGCTGACGCGGCACGGGGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACCTTGGCAACCTCGTATGCCCCGCCAGCTTCGATCTCGGTGAAAAACGCAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAAACAAACCACTGAAATATCCGCA‑TATGt < 2:54030/250‑1 (MQ=255) ccGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTg < 1:513543/249‑1 (MQ=255) cTACCGGAACACCACCGAGTCAGGTGAACACGGTAAAAGGCTGCCAACTTGACCCAAAAAGGATGGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGAt < 2:677575/250‑1 (MQ=255) caagccAGCGATCCAGGGACACACCGGTAAAGGCTTCCACGTGGAGTCACAGACGATGGCCGACGCCGCCCCGCGTCCGCCACTGGACGATAAAGGTATCCTGTTTATCGAAAACGCTGGCACCCTCGTATGCCCGGCCAGCTTCGATCGCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAACCAACTGAAAAAGCCGCA‑TATGTTTGCCGCCGCCACGCTGATGCTGCTc < 2:669265/246‑1 (MQ=255) cacCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGGGC‑TTTTCGTTACCCCAAGGGAAGACCAACCACTCGAAAATTCGCATTTTTTTTGCCGCC‑CCCCCCTGGAGCTGCTcca > 2:779060/1‑248 (MQ=255) agagATTCAGGTGAACACCTGGAACGGCTGCCATCTTGACGCTCAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACTATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACaaa < 2:533873/247‑1 (MQ=255) cGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGGTC‑TTTTCGTTTCCGGAGGGGGAGAAAAACCACTGAAAAAACCGCA‑CATGTTTGTCGCCGCCCCCCTGCTGCTTCTCCACcaaa > 2:407863/1‑248 (MQ=255) aTTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTg < 1:676652/250‑1 (MQ=255) cAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCCTTACCGAAGGGGAAGACAAACCACTGAAAAATCCGCA‑TATGTTTGCCGCCCCCTCGCTGATGCTGCTCAACAAAGTTGAcc > 1:564113/1‑250 (MQ=255) gTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGt < 1:91150/250‑1 (MQ=255) acacCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGcc < 1:573320/250‑1 (MQ=255) cGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAGCACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTAt < 1:591671/250‑1 (MQ=255) gCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACtt < 1:369888/250‑1 (MQ=255) ccATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTg < 2:299599/248‑1 (MQ=255) cATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑Cg < 1:567010/250‑1 (MQ=255) ctttGACGCACAGATGATTGACGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATGCTGTTTATCGAAAACGTTGGCATCCTCGTATGCCCGGCCAGCTTCGATCTCTGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGtt < 2:632412/248‑1 (MQ=255) gtGACGCACATAAAGTTGCCGACGCCACACCGCGCCTGACGCTGGACGATAACTGTATTCTGTTTATCGAAAACGTTGACAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGATACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCTCTGATGCTGCTCAACAAAGTTGCCCTGTTACAGAATCTCaaaagagaaggtg < 2:856260/248‑12 (MQ=255) aCGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGaaa < 2:910058/249‑1 (MQ=255) cacaGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTg < 1:316779/250‑1 (MQ=255) tgatGGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGc < 2:170408/250‑1 (MQ=255) ccGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGTTACCGAAGGGGAAGACAAAACACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGATGACCCGTTTCCGTATCCCCACTTTGA‑CGTTGAAAAATGCATCGCCTgcgc > 1:17152/1‑250 (MQ=255) cGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGAGC‑TTTTCGTTTACCAAGGGGAAGAAAAACAACTGAAATATCCGCA‑TATGTTTGTCGCCGCCCCCCTGACTCTGCTCAACAAAGATGAACTGTTGCCCGTTCTCAACTTTTA‑CGTTGAAAAGTGCATCGCCTTCGcc > 2:861388/1‑250 (MQ=255) aCGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCcg < 1:643834/250‑1 (MQ=255) cACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGGGAAGACAAAACACTGAAATATCCGCA‑TATGTTTTCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCATATCTCAACTTTGT‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAgg > 1:783184/1‑249 (MQ=255) ccGCGACTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCGCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCa < 1:326836/250‑1 (MQ=255) gTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATcc < 2:718083/250‑1 (MQ=255) cTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTg < 1:425094/250‑1 (MQ=255) aCGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTTCCGTT‑CCGGAGGGGAAGACAAACCACTGAAATATCCCCC‑TATTTTTGCCGCCGCCTCGCTGATGCTGCTCAAAAAAAATGACCTGTTGCCGTATCTCAAACTTGA‑CGTTGAAAAAAGCATCGCCTGCGCCCCCG‑AAGGCCAACCAG‑AAATTGAAat > 1:746559/1‑250 (MQ=255) aCGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAat < 2:242717/250‑1 (MQ=255) cGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAat‑c < 1:864467/250‑1 (MQ=255) tAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGTTACCGAAGGGGAAGAAAAAACACTGAAATATTCGCC‑TATGTTTGCCGCCGCCTCGCTGATTCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGGTTAAAAGTGGATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAat‑catc > 1:506938/1‑250 (MQ=255) tAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAat‑catc < 1:598710/250‑1 (MQ=255) aCGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATcc < 1:808285/249‑1 (MQ=255) aCGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGGGAAGACAAACCACTGAAATATCCGCA‑TATGTTTTCCGCCCCCTCGCTGATGCTGCTCAACAAAATTGACCTGTTGCCGTATCTCAACTTTTT‑CGTTGAAAAATGGATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCt > 1:452441/1‑250 (MQ=255) cGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCtt < 1:803160/250‑1 (MQ=255) cGGTACGCTGATTATCGAAAACGTTGGCAACCTCGTAGGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCtt < 2:727023/250‑1 (MQ=255) gTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCCTTTCCGAAAGGGGAGAAAAAACACTGAAAAAATCGCC‑TATGTTTGCCGCCGCCTCGCCGGTGCCGCTCCACAAAATTGACCTGTTGCCGGATTTCAACTTTTG‑CGTTGAAAAAGGCATTCCCCTCGCCCGCC‑CAGTCAATTCAC‑GAAATGaaaatatactttt > 2:441760/1‑240 (MQ=255) aTTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGTTTCCGAAGGGGGAGACAAACAACAGAAATATCCGCA‑TATGTTTGCCGCCGCCCCGCCGGTGGTGCCCCACAAAAATTACCTGTTTCCGGATCTCACCTTTTA‑CGTTTAAAAATGCATCCCCCGCCCCCGCC‑AAGGCAAACCAG‑GAATTTAAaa‑cattcattttt > 2:214238/1‑242 (MQ=255) gtCTGTTTATCGAAAACGTTGGCAACCTCGCATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTAttt < 2:932233/248‑1 (MQ=255) tCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTTCCGAAGGGGAAGAAAAACCACTGAAATATCCCCA‑TATGTTTTCCGCCGCCCCGCTGATGCTGCCCAACAAAATTGACCTGTTTGCGTATCTCAACTTTTACCGTTAAAAAG‑GGATCGCCTCCGCCCGCC‑GAGGCCAACCAGCAAATTGAAA‑‑CATCCCTATTTct > 2:793011/1‑249 (MQ=255) ttATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGGGAAGACAAAACACTGAAAAATCCGCA‑TATGTTTTCCGCCGCCCCGCTGATGCTGCTCAAAAAAATTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTTCGccac > 1:335909/1‑250 (MQ=255) caaaCGTTGGCAACCTCGTCTGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAgcgg < 2:279132/249‑1 (MQ=255) aCGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGG‑CTTCCGCTACCGAAGGGGAAGAAAAACCACTGAAAAATCCGCA‑TATGTTTTTCGCCGCCCCGCTGAGGCCGCCCCACAAAAATGACCTGTTTCCGTATCTCCACTTTTT‑CGCTGAAAAGAGCATCGCCTGCGCCCCCG‑CAGTCCATCCCC‑AACATGAAAA‑CATCCTTTTTTCCGCCACCCgcggcg > 2:53579/1‑249 (MQ=255) aCGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGa < 2:626314/250‑1 (MQ=255) cGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTTCCGCAGGGGAAGACAAACCACTGCAAAATCCGCC‑AATGTTTGCCGCCCCCTCGCTGATGCTGCTCCAAAAAAATGGCCTGTTGCCGTATCTCAAATTTTA‑CGTTTAAAAGAGCCTCGCCCGCGCCCGCC‑AAGTCAAACCAA‑CAATTGAAATACTTCCTCTTTCCCGCC‑CCAccggcg > 2:726212/1‑248 (MQ=255) tGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGGGC‑TTTCCGTTACCGCAGGGGGAGAAAAAACACTGAAATATCCGCA‑TAATTTTTTTGCCGCCTCCCCGGTGCTGCTCCACAAAAATGACCTCTTGTCCCATCTCCATTTTTC‑TGTTGCAAAGAGGATCGCCTGCGCCCCCG‑GAGTCAAACTAG‑CAATTGAAAT‑CAACCTTCTTTCCTCTCCCACCGGCGAgggg > 2:70215/1‑250 (MQ=255) gCAACCTCGTATGCCCGGCCAGCTGCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTAAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGAt < 1:584131/250‑1 (MQ=255) cAACCTCGGATGCCCGGCCAGCTTCGATCTCGGGGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGATg < 2:600978/250‑1 (MQ=255) tCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGGTACCGCAGGGGGAGACAAAAAACAGAAAAATACGCA‑TATTTTTTCCGCCGCCCCGCGGATACCGGTCACCAAAAATGACCTGTTGCCCGATTTCAACTTTGA‑CGTTGTAAACAGCATCGCCCGCGCCCGCGCAAGTAAATCCA‑‑AAATTGAAAT‑CATCCCTTTTTATGCCACCCCCGGCGAAAGGAGGGActt > 2:963022/1‑248 (MQ=255) tATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTg < 1:533153/250‑1 (MQ=255) gCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGTTTTCGAAGGGGGAGACAAACCACCGAAATATCCGCA‑TATGTTTTCCCCCCCCCCGCCGATGCTGCTCAACAAAATTGACCTGTTGCCCTATCTCCACTTTTA‑CGTTTTAAAGTGCATCGCCTGCGCCCGCG‑AAATCAATCCAG‑AAATTTAAAT‑CATCCTTTTTTCCGCCCCCCCCGGCGAAGGGGTGGGCCAGGGGCttgacttgcc > 2:116017/1‑241 (MQ=255) gATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGGGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAATTGACCTGTTGCCGTATCTCAACTTTTA‑CGTTGAAAAGTGCATCGCCCGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGggga > 1:440739/1‑250 (MQ=255) tctcGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGTTAACGAAGGGGAAGACAAACCACTGAAAAATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCCGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGGCCAGTGGCTGAACTGGCTGGAGaca > 1:721422/1‑250 (MQ=255) ggTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGc < 1:8917/250‑1 (MQ=255) tGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGa < 1:692275/250‑1 (MQ=255) aaaCACAAAGGGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGAtgtg < 2:513912/250‑1 (MQ=255) aaGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGc > 1:53835/1‑250 (MQ=255) aaGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGc < 2:952913/250‑1 (MQ=255) tCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAg < 2:824981/248‑1 (MQ=255) ccGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGAt < 1:235546/249‑1 (MQ=255) ccGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATc > 1:460929/1‑250 (MQ=255) ttACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCACGGCCAGATCCg > 1:782611/1‑249 (MQ=255) aCCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCa < 2:82349/249‑1 (MQ=255) gAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCACCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCAt > 1:168824/1‑250 (MQ=255) aaGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCAtt < 2:732583/250‑1 (MQ=255) | AGTTATTGAAGGCGACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCA‑TATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGA‑CGTTGAAAAGTGCATCGCCTGCGCCCGCG‑AAGTCAATCCAG‑AAATTGAAAT‑CATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATT > NZ_CP009273/2844764‑2845258 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |