Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,036,353	0	C	T	35.9%	53.1 / 12.8	53	F193F (TTC→TTT)	srkA	stress response kinase SrkA
Reads supporting (aligned to +/- strand):  ref base C (23/11);  new base T (19/0);  total (42/11)
Fisher's exact test for biased strand distribution p-value = 4.48e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGA‑TTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT‑GCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGT  >  NZ_CP009273/4036115‑4036598                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                        tCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCttttttcg                                                                                                                                                                                                                                              >  1:791689/1‑245 (MQ=255)   gAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGc                                                                                                                                                                                                                                            <  2:950385/250‑1 (MQ=255)    aaGCTGATAATATCGATCAGATGGAAGCGGTTGGTCGTTATTTAGGGCGTATGCACCAGCGGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCt                                                                                                                                                                                                                                           <  2:763305/250‑1 (MQ=255)       cTGATAATAGCGATCAGATGGAAGCGGTTGTGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCGCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACa                                                                                                                                                                                                                                        <  2:380794/250‑1 (MQ=255)                  gATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTACCGTTCTGCGGCTCCATGGAGACCGcc                                                                                                                                                                                                                             >  2:408971/1‑250 (MQ=255)                    tCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCCTTCTTCGGCTACCTGGAGACTGcccc                                                                                                                                                                                                                           >  1:808765/1‑248 (MQ=255)                         tGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAGTTTACCGTTCTGCGGCTCCATGGCGAATGCCACGCCgg                                                                                                                                                                                                                      >  2:51159/1‑250 (MQ=255)                               cGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGCAGATTTCACCGCTCTGCCGCTACCTGGAGGATGTCCCGCCGGGGatat                                                                                                                                                                                                                >  1:54121/1‑250 (MQ=255)                               cGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGGGACTGCCACGCCGGGGatat                                                                                                                                                                                                                >  1:131785/1‑250 (MQ=255)                                       cGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGAATTTTCCGTTCTTCGGGCTCCTGGAGAACGTCACCCCGGGAGAATTTTCTGgc                                                                                                                                                                                                        >  2:270597/1‑250 (MQ=255)                                       cGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATTGAGACTGCCACGCCGGGGATCTTCTCTTgc                                                                                                                                                                                                        >  2:270623/1‑250 (MQ=255)                                         ttATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCACATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTCCCGGTCTGCGGCTTACTGGGGACTGACAACCCCGGAATATTTTCTGgcgc                                                                                                                                                                                                      >  2:219546/1‑250 (MQ=255)                                             ttAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGCTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTTTCTGGCGCGCTg                                                                                                                                                                                                  >  1:738638/1‑250 (MQ=255)                                                         cACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTAACGTTCTGCGGCTACATGGGGACCGCCACGCCCGGAATATTTTCTTGCGCGGTGGTCCAAtgtttg                                                                                                                                                                                      >  1:586134/1‑250 (MQ=255)                                                          aCCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACCAGGAGACTGCCCCGCCGGGGATATTCTCCTGCGCCATGGGGCCAtgtttgt                                                                                                                                                                                     >  1:807091/1‑250 (MQ=255)                                                              gACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGAt                                                                                                                                                                                 <  1:245995/250‑1 (MQ=255)                                                                              cTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTa                                                                                                                                                                 <  2:912383/250‑1 (MQ=255)                                                                                 tttATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTCAACGTTCTGCGGCTACCTGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGGTGGGCCAATGATTGTTGATCTGGATGATGCACGCAATg                                                                                                                                                              >  1:730795/1‑250 (MQ=255)                                                                                         tCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGGTTTCACCGTTTTGCGGCTACACGGAGACTGCCACCCCGGGGATATTCTCTGGTGCGATGGTCCCCTGTTTGTTGGTCTGGATGATGCACGTAATGGTTCAGcc                                                                                                                                                      >  2:527111/1‑250 (MQ=255)                                                                                          cGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTTCCGTTTTGCGGCTACATGGGGACTGCCCCGCCGGGGATATTTTCTGGCGCGATGGGCCACTGTTTGTTGATCCGTAGTATGCACGTAATAGGCCAGCCg                                                                                                                                                     >  1:139178/1‑250 (MQ=255)                                                                                                aCCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATTGAGACTGCCACGCCGGGGATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTTGATGATGCACGTAATGGTCCAGCCGTTCAgg                                                                                                                                               >  1:261371/1‑250 (MQ=255)                                                                                                 ccATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTACCGCTCTTGGGCCACATGGAGACTGCCACGCCGGGGATATTTTCTGGCGCGGTGGGCCAATGTTTGTTGATCTGGGTGGTTCACGGAATGGGCCAGGCGTTCCggg                                                                                                                                              >  2:748676/1‑249 (MQ=255)                                                                                                   aTCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAAATTTTCCCGTTCTGGGGCTACATGGGGACTGCCACGCCGGGGATATTTTCTGGCGCGATGGGCCCATGTTTGTTGGTCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGG‑tt                                                                                                                                           >  1:167458/1‑250 (MQ=255)                                                                                                       gTTTGAACGAATATCTCACTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTCACCGTTCTTCGGCTACATGGAGGCTGCCACGCCGGGGATATTCTCTGGGGCGGTGGTCCCAAGTTTGTTGGTCTTGGTTGTGCACGGAATTGTCCCAGCGTTTAGGG‑TTtgtg                                                                                                                                       >  1:484224/1‑250 (MQ=255)                                                                                                           gAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTTCGGCCACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGACGGGCCAATGTTTGTTGTTCTGGATGATTCACGTAATGGGTCAGCCGTTCCGGG‑TTTTTGGGTg                                                                                                                                   >  1:689397/1‑250 (MQ=255)                                                                                                           gAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGA‑TTTGTGGATg                                                                                                                                   >  1:757615/1‑250 (MQ=255)                                                                                                                   atCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGGCCAGCCGTTCAGGA‑TTTGTGGATGTTGCTCaa                                                                                                                           >  1:605647/1‑250 (MQ=255)                                                                                                                       cATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCCTTCTTCGGGTACATGGAGAATGCCACCCCGGGAGTATTCTCTTGGGCGATGGGCCCATGGTTTTTGATCTGGATGATGCACGTAATGGGCCAGCCGTTCAGGAGTTTGTGG‑TGTTGGTCAATTGc                                                                                                                       >  2:68266/1‑250 (MQ=255)                                                                                                                          tGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGCTGGTCCCATGTTTGTTGATCTGGATGATGCACGGAATGGTCCAGCCGTTCAGGA‑TTTGTGGGTGTTGCTCCATGGCgg                                                                                                                     >  1:723437/1‑248 (MQ=255)                                                                                                                              ccACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGATGGTCCCATGTTTTTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGG‑TTTGTGGGTGTTGCTCAATGGCGATaaaa                                                                                                                >  1:470893/1‑249 (MQ=255)                                                                                                                                aCGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAGTTCACCGTTTTGCGGCTACATGGAGACTGGCACGCCGGGGATATTTTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGG‑TTTGTGGGTGTTGCTCAATGGCGGTAAAGcc                                                                                                              >  1:210756/1‑250 (MQ=255)                                                                                                                                   cAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTACCGTTCTGCGGCTACATGGGGACCGCCACGCCGGGGATATTTTCTGGCGCGGTGGTCCCATTTTTTTTTTTCTGGATGATGCACGGAATGGTCCAGCCGGTCAGGG‑TTTTTGGATGTTGTTCAATGGCGATAAAAcccgg                                                                                                           >  2:574119/1‑247 (MQ=255)                                                                                                                                      gCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGGTGGTCCCATGTTTTTTGGTCTGGGTGATGCACGTAATGGTCCAGCCGGTCAGGG‑TTTGTGGATGTTGCTCAATGGCGATAAAGCCGagcag                                                                                                        >  1:542394/1‑250 (MQ=255)                                                                                                                                        tGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCTCCGTTCTGCGGACACATGGAGACTGCCACCCCCGGGATATTCTCTGTCGCGCTGGGCCACTGTTTGTTGCTCTGGGTGGTGCACGTAATGGGCTCGCCGCTTAGGG‑TTTGTGGGTGTTTGTCCATGGGGATAAAgtgacgccgcg                                                                                                      >  2:14109/1‑240 (MQ=255)                                                                                                                                                gACGCTACACTGAGCCCGTCCGGGTTGAAAGCGGAATTCCTGAAAGTGACAGATGACCTGATGGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGA‑TTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT‑GCAAc                                                                                             <  2:360710/250‑1 (MQ=255)                                                                                                                                                  cGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGATGGGCCCATGTTTGTTGGTCTGGGTGATGCCCGTAATGGTCCAGCCGTTCAGGA‑TTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT‑GCAACTg                                                                                           >  2:25653/1‑250 (MQ=255)                                                                                                                                                  cGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGA‑TTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT‑GCAACTg                                                                                           <  2:163892/250‑1 (MQ=255)                                                                                                                                                     tACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTTCCGTTCTGCGGCTACATGGAGACTGCCACCCCGGGGATATTTTCCGGGGCGGGGGTCCAAAGTTTGTTGGTCTGGGTGGTGCACGGAATGGTCCAGCCGGTTAGGG‑TTTTTGGGTGTTGGTCAATGGGGATAAAGCCCGGCAGCGGGT‑TGAACTggg                                                                                         >  2:304324/1‑248 (MQ=255)                                                                                                                                                        gcTGATACCGTCCGGGTTGAAAGCGGCTTTCCGGAAAGCAACAGAGGAGCTGTTTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGA‑TTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT‑GCAACTGGAAACt                                                                                     <  2:769639/249‑1 (MQ=255)                                                                                                                                                             tACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGA‑TTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT‑GCAACTGGGAACtattat                                                                                >  1:266448/1‑250 (MQ=255)                                                                                                                                                              aCCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTCCCCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTGGCGCGGTGGGCCAATGTTTGTTGATTTGGGTGATGGACGGAATGGTCCAGCCGTTCAGGA‑TTTGTGGATGTTGCTCAATGGCGATAAAGCCGCGCAGCGGAG‑GCAACTGGGAACTattatt                                                                               >  2:964132/1‑250 (MQ=255)                                                                                                                                                                cTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGA‑TTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT‑GCAACTGGAAACTATTATTGa                                                                             <  1:339/250‑1 (MQ=255)                                                                                                                                                                  tCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTTGCGCGATGGTCCCATGTTTGTTGATCTGGGTGATGCACGGAATGGTCCAGCCGTTCAGGA‑TTTGTGGGTGTTGCTCCATGGCGATAAAGCCGAGCAGCGGGT‑GCAACTGGGAACTATTATTTAAg                                                                           >  1:894561/1‑250 (MQ=255)                                                                                                                                                                                    ttCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCCACATGGAGACCGCCCCGCCGGGGATATTCTCTGGCGCGGTGGGCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGG‑TTTGTGGGTGTTGGTCAAAGGCGGTAAAACCGAGCCGCGGAG‑GCAACTGGAAAATTTTATTTAACGGTATGAAGAATTTaagt                                                         >  2:158484/1‑247 (MQ=255)                                                                                                                                                                                      ccTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTGGCGCGGTGGTCCAATGTTTGTTTATCTGGATGATGCACGTAATGGTCCAGCCGGTCAGGA‑TTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT‑GCAAATGGGAACTATTATTGAAGCTTATTAAGAATTTTGCGAg                                                       >  1:953390/1‑250 (MQ=255)                                                                                                                                                                                        tGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCt                                                                                                                                                                               >  1:126017/1‑130 (MQ=255)                                                                                                                                                                                         gAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTg                                                                                                                                                                              <  2:126017/130‑1 (MQ=255)                                                                                                                                                                                          aaaGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACCCCGGGGATATTTTCTGGCGCGGTGGTCCCATGTTTTTTGATCTGGGTGATGCACGTAATGGTCCAGCCGGTCCGGG‑TTTGTGGGTGTTGCTCCATGGCGGTAAAGCCGAGCAGCGGGTGGCAATTGG‑AACTCTTATTTTAGCTTATGTAGAATTTAGCGGGGTCg                                                   >  2:31308/1‑250 (MQ=255)                                                                                                                                                                                            gccgccAGATGGGTTAATTGCCGCCGTTACAGCCCACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGA‑TTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT‑GCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGac                                                 <  2:492567/245‑1 (MQ=255)                                                                                                                                                                                                                             acacTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTTGCGCGATGGGCCCATGTTTGTTGATCTGGATGATGCACGTAAAGGTCCAGCCGTTCAGGA‑TTTTTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT‑GCAACTGGAAACTATTATTTAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTAcgc                >  1:17723/1‑250 (MQ=255)                                                                                                                                                                                                                                       gAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGA‑TTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT‑GCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGtt      >  1:440661/1‑250 (MQ=255)                                                                                                                                                                                                                                          gATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGA‑TTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT‑GCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTgg   >  1:334448/1‑250 (MQ=255)                                                                                                                                                                                                                                           aTTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGA‑TTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT‑GCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGt  >  1:242776/1‑250 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                        TCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGA‑TTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT‑GCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGT  >  NZ_CP009273/4036115‑4036598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑