Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,245,9890CT35.1% 83.5 / 16.3 58E136K (GAA→AAA) plsBglycerol‑3‑phosphate 1‑O‑acyltransferase PlsB
Reads supporting (aligned to +/- strand):  ref base C (6/31);  new base T (20/0);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.90e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCG  >  NZ_CP009273/4245747‑4246226
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               
cAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGcccc                                                                                                                                                                                                                                          <  1:525995/250‑1 (MQ=255)
  gATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCTGTTAA‑GCATACGCAGCGGC‑GGG‑TTCTCTTCGCCTTTT‑TTCCGcccccg                                                                                                                                                                                                                                        >  2:110989/1‑248 (MQ=255)
   acccTGCCGGGCAGGAAACCGTGGGCCTACGGCAGCCAGCCGTTGCCGGGCAAGGTGCATACGCGCCCCGCGCCCCATTTTCTGGGGGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGgc                                                                                                                                                                                                                                       <  2:730688/248‑1 (MQ=255)
           gAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCCCTTCGCCCTTT‑TTCCCCCCCCGCGCGCGccc                                                                                                                                                                                                                               >  2:363318/1‑250 (MQ=255)
               aGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAAc                                                                                                                                                                                                                           <  1:474669/250‑1 (MQ=255)
               aGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑GTCACTTCGCCTTTT‑TTACGCCCCCGCCCGCGGCCcaa                                                                                                                                                                                                                            >  2:745997/1‑249 (MQ=255)
               aGGCAAACGTGGGCCTACGGCAGCCAGACGTAGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAAc                                                                                                                                                                                                                           <  2:925350/250‑1 (MQ=255)
                       gTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TTCTCCCCCCGCGCGGCACCCAACA‑AAACCGa                                                                                                                                                                                                                  >  1:915594/1‑250 (MQ=255)
                                     gCCAGAGGTTGAGTGGCAAGGTGCATCGCCCCCACGCGCCCCAGTTGCTGGGCGAAAGTTTTACCCGGCCCGTT‑TCCCCCCGCCATCCGGCGCAGCGAAACGGACGGCGAGAACCGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCt                                                                                                                                                                                                    <  2:181013/250‑1 (MQ=255)
                                           cGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTTACTTCGCCTTTT‑TCACTCCCCCGCGCGGGACCCAACA‑ACCCCCACACCGGCCCCCTCTGcaaa                                                                                                                                                                                               >  1:684079/1‑247 (MQ=255)
                                             ttGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑GTTCCTTCGCCCTTT‑TTACGCCCCGGCGCGCGACCCAACA‑TCACCCACCCCGGCACCATCTGcaccccc                                                                                                                                                                                            >  1:633524/1‑246 (MQ=255)
                                                     cAAAGTCCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTgg                                                                                                                                                                                   <  1:614134/250‑1 (MQ=255)
                                                       aaGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGt                                                                                                                                                                                 <  1:424385/250‑1 (MQ=255)
                                                                 gcgcCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑CTCACCTTCCCTTTT‑TCACGCCCCGGCGCGCGACCCAAAA‑ACACCCACACCGGCACCCTCTTCCAATACAG‑AATTTTGTTGCTCCggag                                                                                                                                                                       >  1:326677/1‑248 (MQ=255)
                                                                   gcCACGCGCGCCAGTTTCGGAGCGATAGTTTTACCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCa                                                                                                                                                                     <  1:681620/250‑1 (MQ=255)
                                                                     cgcgcgccCCAGTTCCTGACCGAAATTTTGATCCGTGCGGTG‑TCCATCCGCCATACGGCACAGCGAATCTGACGGCGAGAAACGCATAAACCTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCaaa                                                                                                                                                                   <  2:459242/248‑1 (MQ=255)
                                                                       cgcgcgCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑GTCAATTCTCCTTTT‑TTTCCCCCCGGCCCGCGGCCAAACA‑ACACCGCCCCATGCCACAACTGCACACCCAG‑ATTTGGGTTTCTAAGGTTCacaac                                                                                                                                                                 >  2:488543/1‑246 (MQ=255)
                                                                        gcgcgcCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑GTCACTTCGCCTTTT‑TTTCACCCCCGGCCGCGACCAAACA‑TCAACCACCATGCGCCCATCTCCACCACCAGCATTTGGGTTG‑TCCGGGGCaaaac                                                                                                                                                                 >  2:703018/1‑247 (MQ=255)
                                                                        gagccgcatttttTGAGAAATTGTTTCACCCGT‑CCGTGTTTCACCCTCAATACGCCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCTCTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAGCA‑GCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAAttg                                                                                                                                                                <  2:811023/241‑3 (MQ=255)
                                                                         cgcgcCATTTTCGGAGCGATATTTTTACCCGGGCCGTG‑TTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCAAAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCga                                                                                                                                                               <  2:817940/250‑1 (MQ=255)
                                                                           cgcCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTTACTTCGCCCTTT‑TTTCGCCCCCGCGCGCGGCCCAACA‑TCCCCCCCCCTCGCCCCCCCTCCCCCTCCCG‑ATTTTGGGTGCTCCGGGGCAAAta                                                                                                                                                                 >  2:581188/1‑245 (MQ=255)
                                                                           cgcCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑GTCACTTCGCCTTTT‑TTACGCCCCCGCGCGCGACCAAAAA‑TCACCCACACTGGCACCATCTGCCCATCCCG‑ATTTTGGTTGCTACCGTGCAAAACgag                                                                                                                                                              >  1:585292/1‑249 (MQ=255)
                                                                           cgcCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑GTCACTTCCCCTTTT‑TTACCCCCCCGCGCCCGCCCCAAAA‑TCACCCCCACCTGCCCCCTACTCACCTCCCC‑ACTTGTGGTGCTACGTTGCaaaac                                                                                                                                                                 >  2:825973/1‑244 (MQ=255)
                                                                                       aGCGATAGTTTTACCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTgg                                                                                                                                                    <  1:820312/247‑1 (MQ=255)
                                                                                         cGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑GTCACTTCGCCTTTT‑TTACGCCCCCGCCCGCGACCCAAAA‑TCACCCACACCGGCCCCCTCTTCCCATCCAC‑ACTTGGGGTGCTACCGTGCCAAACCGGATACGCGTgggaaaa                                                                                                                                               >  2:707188/1‑245 (MQ=255)
                                                                                            tAGTTTTACCCGGGCGGTG‑TCCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCtt                                                                                                                                            <  2:795807/250‑1 (MQ=255)
                                                                                             aGTTTTACCCGTGCCGTG‑TTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTa                                                                                                                                           <  1:880651/250‑1 (MQ=255)
                                                                                                  tATCCGTGCCGTG‑TTCAACCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAg                                                                                                                                       <  1:661160/249‑1 (MQ=255)
                                                                                                   ccccggcccgtg‑CTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGa                                                                                                                                      <  2:339711/242‑1 (MQ=255)
                                                                                                            gtg‑tTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGCGGGG‑TCCATTTCCCCTTTT‑T‑TCCCCCCCGCGCCCCGCCACACA‑ACACCCCCACCGGCCCCACCCGCCCCTCCCC‑TTTTGTGGTGCTACGGTGCCAAACGTCGTAGTCGTGGAAACGCTTAATATAATCTTCtttt                                                                                                                            >  2:1342/1‑249 (MQ=255)
                                                                                                              g‑tTCATCGGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGCGGAACAGCTTAATAGACTCTTCTTTCgg                                                                                                                          <  1:436563/250‑1 (MQ=255)
                                                                                                                tccATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAACCGCACAAAACTTTCGCGACCGAGCCACATTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCgg                                                                                                                          <  2:706853/247‑1 (MQ=255)
                                                                                                                  cATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCg                                                                                                                        <  1:581188/249‑1 (MQ=255)
                                                                                                                  cATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑GTCACTTCGCCCTTT‑TTTCCCCCCCGCGCGCGGCCAAACA‑TCCCCCACACCCGCACCCCCTGCACACCCCC‑CTTTGGGGTTCTACGGGGCAAAACGAGAGATGCTTGGAAACGATTATAAGAATCTTCttttcgggt                                                                                                                       >  2:796352/1‑244 (MQ=255)
                                                                                                                  cATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑CTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCCGCACACCCAG‑ATTTGGGTTTCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCCTCCTTCGGCgt                                                                                                                       >  1:462263/1‑250 (MQ=255)
                                                                                                                       gCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TTACGCCCCCGCGCGCGACCCAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTTGGGTGCTACGGTGCAAATCGAGATAGTCGTGGGAAAGCTTAATAGACTCTTCTTTCGGCGTGTaa                                                                                                                   >  1:215513/1‑249 (MQ=255)
                                                                                                                        ccATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TTACGCCCCCGCGCGCGACCCAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTTGGTTGCTACGGTGCAAAACGAGATAATCGTGGGACAGCTTAATAAACTCTTCTTTCGGCGGGTaa                                                                                                                   >  1:781917/1‑248 (MQ=255)
                                                                                                                            aCGGCGCCGCGAACCTGACGGCGAGAAACGCACAAACCTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTg                                                                                                             <  2:141608/250‑1 (MQ=255)
                                                                                                                              ggCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGaa                                                                                                           <  1:742373/250‑1 (MQ=255)
                                                                                                                                ggcAGGGAAACGGACGGAGAAAAACGCACAAAGCTGTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAaca                                                                                                         <  2:197159/249‑1 (MQ=255)
                                                                                                                                cgcAGCAAAACTGACGGCGAAAACCGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAaca                                                                                                         <  2:882270/250‑1 (MQ=255)
                                                                                                                                  cAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAacaca                                                                                                       <  1:703018/250‑1 (MQ=255)
                                                                                                                                   aGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAacacac                                                                                                      <  1:587073/250‑1 (MQ=255)
                                                                                                                                        aaCTGACGGCGAGAAACGCACAAAACTGCCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGc                                                                                                 <  2:718478/250‑1 (MQ=255)
                                                                                                                                           tGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑GTTACTTCGCCTTTT‑TTACGCCCCCGCGCGCGACCAAACA‑TCACCGACACTGGCACCCTCTGCACATCCAG‑ATTTGGGGTGCTACGGTGCAAATCGAGATAGTCGGGGAACAGCTTAATAGAATCTTCTTTCCGCGTGTAATAGGTGAACACACGCGGcccc                                                                                              >  1:407570/1‑249 (MQ=255)
                                                                                                                                                                  tGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑GTCACTTCGCCTTTT‑TTACGCCCCCGCGCGCGGCCAAAAA‑ACACCCACCCCGGCCCCATCTGCACAACCAG‑ATTTTGGGTGCTACGGGGCAAAACGAGATAATTGTGGAAAAGCTTAAAAAAACCTTCTTTCTGCGTGGAATAAGTGAAAAAAACCCGCCCGCCGGGAATGAacaacaaaagcgg                                                                       >  2:954313/1‑240 (MQ=255)
                                                                                                                                                                      gccaccgccccAAAGCACAGCAAAAATTTTCTGCCCCCCTTTAA‑CCACCCCCAG‑GGG‑GGG‑TTCCTCTCCCCTTTTCCCCCCCCCCGGCGCCCCACCAAACA‑CCCCCGCCACTGGCACCTTCTGCACATCCAG‑ATTGGGGTTGCTAGGGTGCAAATCGAGATAGTCGGGGAACAGCTTAAAAGACTCTTCTTTCGGCGTGAAATAGGGGAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGt                                                                   <  2:103555/241‑1 (MQ=255)
                                                                                                                                                                           cGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑GTCACTTCGCCTTTT‑TTTCGCCCCCGCGCGCGACCCAACA‑TCACCGACACCCGCACCATCTGCACATCCAG‑CTTTGGGGTGCTACGGGGCAAATCGAGCTAGACGTGGGACAGCTTAATAAACTCTTCTTTCGTCGGGTAATATGGGGACACACGCCGCCCGCCCTGAGTGAACCCATAGAGCgggcggatcgg                                                              >  2:607727/1‑241 (MQ=255)
                                                                                                                                                                                       cgacaaaatttttCTGCACGCAGTTAA‑CGACGCGCAGCGGG‑GGT‑TCCTCTCCCCCTTTT‑CCCCGCCCGGCCCGGCGA‑CAAACACCCACCGCCACTGCCACATTCGGCACACCCAG‑TTTTGGGTTGCTGGGGGGCAAAGCGAGAGAGTGGGGGAACAGCTAAATAGACTCTTCTTTCGGCGTGAAAGGGGTGACCACGCGGGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTc                                                  <  2:595249/240‑1 (MQ=255)
                                                                                                                                                                                            aaaaaTTTCCGTCCCGCGTTAACGCACCCGCCGCGGG‑GGG‑TCCCCCTCGCCTTTC‑TCCCCCCCCGGCCCCCGACAAAACA‑CCCCCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGGAAAAATCGAGATGGTCGGGGAAAAGCTTAATGGACTCTTCTTGCGGGGTGAAAGAGGTAAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGc                                              <  2:607384/250‑1 (MQ=255)
                                                                                                                                                                                             aaaaTTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑GTCACTTCGCCTTTT‑TTACCCCCCCGCGCGCGACCAAACA‑TCACCGACACCGGCACCATCTGCACATCCAG‑CTTTGGGTTGCTACGGTGCAAATCGCGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCTGCGTGCAATAGGTGAACACACGCGGCCCGCCGTGAAAGAACACCAATCGCGGCAGTAGCGTGCCGTCGAttttccgcag                                            >  2:744466/1‑243 (MQ=255)
                                                                                                                                                                                                     tGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑GTCACTTCTCCCTTT‑TTACGCCCCCGCGCGCGGCCCAACA‑ACACCCACACTGGCACCCTCTCCCCATCCCG‑ATTTGGGTTGCTACGGGGCAAATCCAGATAGTCGTGGGACAACTTAATAGACTCTTCTTTCTGCGTGTAATAGGTGAAAACACGCGGCCCGCCGTGAATGAACACATAACGCGGCAGTAGCGGGCCGTCGATTTTCCGCgggccctaac                                    >  2:183704/1‑242 (MQ=255)
                                                                                                                                                                                                                aa‑GCATACGCCGCGGC‑GGG‑TTCACTCCGCTTTTT‑CCACCCCCCGGCGCGCGCCAAAACA‑TCCCCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTGCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAg                         <  1:545569/250‑1 (MQ=255)
                                                                                                                                                                                                                     aTACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TTCCGCCCCCGCGCGCGACCCAACA‑TCACCGCCACTGGCACCCTCCGCACATCCCG‑ATTTGGGTTGCTACGGTGCAAATCCAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAAGTGAACACACGCGGCCCGCCGGGAAATAACACATAACGCGGCCGTAGCGTGCCGTCGGGTTCCAACGGCGCTTACGGGGAAGGCaacgcat                     >  2:480172/1‑245 (MQ=255)
                                                                                                                                                                                                                            gcggg‑gGGTTTCCCTTCGCCTTTT‑CCCCCCCCCGCCCGCCGCCAAAACC‑CCCCCGACACTGGCACCATCGGCCCACCCAG‑TTTTGGTTTGCTGCGGTGCAAATGGAGATAGTCGTGGAACAGCTAAATAGACTCTTCTTCCGGCGTGTAAGAGGGAACCCCCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCa               <  2:705177/250‑1 (MQ=255)
                                                                                                                                                                                                                              ggc‑ggG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGa             >  1:114405/1‑249 (MQ=255)
                                                                                                                                                                                                                                      ttCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTggg      >  1:102335/1‑250 (MQ=255)
                                                                                                                                                                                                                                          cTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgcgcg  >  2:224929/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               
CAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAA‑GCATACGCAGCGGC‑GGG‑TTCACTTCGCCTTTT‑TCACGCCCCGGCGCGCGACCAAACA‑TCACCGACACTGGCACCATCTGCACATCCAG‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCG  >  NZ_CP009273/4245747‑4246226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑