Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,852,8050TG33.9% 106.4 / 11.5 59V785G (GTT→GGT) mutSDNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  ref base T (14/25);  new base G (20/0);  total (34/25)
Fisher's exact test for biased strand distribution p-value = 5.97e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAGTCTTTTGCTATCGGGAATATTAACG  >  NZ_CP009273/2852557‑2853054
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         
cGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTGCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCAGCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGtt                                                                                                                                                                                                                                                          <  1:469281/250‑1 (MQ=255)
 gATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGtta                                                                                                                                                                                                                                                         <  1:638643/250‑1 (MQ=255)
     gTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGttatta                                                                                                                                                                                                                                                      <  2:404132/249‑1 (MQ=255)
             cTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCAc                                                                                                                                                                                                                                             <  1:565175/250‑1 (MQ=255)
                 cgtgggcgtgCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGc                                                                                                                                                                                                                                          >  1:614645/1‑249 (MQ=255)
                               gAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCCGCTCTGGCCGGGGGGGCAAAAGAGGGTATTAAGCGCGCACCGCCAAAGCTGGGTGAGc                                                                                                                                                                                                                           >  2:569240/1‑250 (MQ=255)
                               gAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCCCTGGGCGGCGGGCCAAAAGAGGGTCTTTAACGCGCCCGGCAAACACCGCCTGAGc                                                                                                                                                                                                                           >  2:92970/1‑250 (MQ=255)
                                 aaaTCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTg                                                                                                                                                                                                                         <  1:355598/250‑1 (MQ=255)
                                        gCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCCGGCGTGCCCAAAGAGGGTATTAAGCGCGCCCGGCGCAAGCTGCGTGAGCTGGAAAGCa                                                                                                                                                                                                                  >  2:208539/1‑250 (MQ=255)
                                        gCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAg                                                                                                                                                                                                                    <  2:57515/248‑1 (MQ=255)
                                        gCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCa                                                                                                                                                                                                                  <  1:396051/250‑1 (MQ=255)
                                        gCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCCCTGGCCGGCGTGCCAAAAGAGGTTATTAAACGCGCACGGGAAAAGCTGCCTGCGCTGGAAAggc                                                                                                                                                                                                                  >  1:228800/1‑248 (MQ=255)
                                             tAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCg                                                                                                                                                                                                             <  2:384228/250‑1 (MQ=255)
                                             tAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCg                                                                                                                                                                                                             <  1:704510/250‑1 (MQ=255)
                                             tAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGGTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCg                                                                                                                                                                                                             >  1:152401/1‑250 (MQ=255)
                                             tAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCCACGGCCTGGCGGTTGCAGCGCTTGCCGGCGTGCCAAAAGAGGGTATTAAGCGAGCCAGGCAAAAGCTGCGTGGGCGGGCAAGCAttttg                                                                                                                                                                                                             >  2:631789/1‑248 (MQ=255)
                                              aaGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCCAAAGAGGGTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGc                                                                                                                                                                                                            >  1:772717/1‑250 (MQ=255)
                                                        cATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAAcgccgc                                                                                                                                                                                                  <  2:614645/250‑1 (MQ=255)
                                                           tGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTTCCGCCCTGGGAGGCGGGCCCAAAGAGGGTATTTAGCGCGCACGGGCAAAACAGCGGGAGGTGGGAAACAATTCGCCGGAagacgcccc                                                                                                                                                                                               >  2:15601/1‑248 (MQ=255)
                                                             aCGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCCGGCGGGCCAAAAGAGGGTATTAAACGCGCACGGCAAAAAATGCGTGAGCTGGGAAGAATTTTGCCGAAcggccccgcgc                                                                                                                                                                                             >  2:364319/1‑248 (MQ=255)
                                                              cGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGTAGCCCCGGCCGGCGTGCCCAAAGCGGTTATTAAGCGCGGACGGCCAAAGCAGCGCGCGCTGGCAAGATCTTTGCTGAAcgccgccgcgcc                                                                                                                                                                                            >  2:436540/1‑247 (MQ=255)
                                                                 tATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGGTAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGc                                                                                                                                                                                          >  1:15927/1‑249 (MQ=255)
                                                                   tttGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGGGGGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGATGCGGGAGCTGGAAAGCAATTCGCCGAACGCCGCCGCTACGCAAg                                                                                                                                                                                       >  1:46651/1‑250 (MQ=255)
                                                                       cTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGGTATTAAGCGCGCACGGCAAAAGCTGCGCGAGCTGGAAAGCATTTTGCCGAACGCCGCCGCTACGCAAGTg                                                                                                                                                                                     >  2:174299/1‑248 (MQ=255)
                                                                         aCCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCCCTGGGAGGCGGGCCCAAAGAGGGTATTAAGAGCGCACGGCCAAAGCTGCGGGAGCTGGGAAGACTTTTGCCCAACGCCGCCGCCACGCaaacgggtg                                                                                                                                                                                 >  2:695751/1‑243 (MQ=255)
                                                                                    cGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAAt                                                                                                                                                                       <  2:560798/249‑1 (MQ=255)
                                                                                     gAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCCAAAGAGGGTATTAAGCGCCGCCGGCAAAAGCTGCGTGAGCTGGAAAGCAATTCGCCGAACGCCGCCGCCCAGCAAGGGGGTGGGACGCAAAAGt                                                                                                                                                                     >  2:174622/1‑250 (MQ=255)
                                                                                     gAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACGGGAGCACGGCGACACCATTGCCTTTATGCCCAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGt                                                                                                                                                                     <  2:759080/250‑1 (MQ=255)
                                                                                      aGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTc                                                                                                                                                                    <  2:258550/250‑1 (MQ=255)
                                                                                        cTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTACCGTGCATCGCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCtt                                                                                                                                                                  <  2:450980/250‑1 (MQ=255)
                                                                                           aCCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGc                                                                                                                                                               <  2:52813/250‑1 (MQ=255)
                                                                                             ccAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTg                                                                                                                                                             <  1:79941/250‑1 (MQ=255)
                                                                                               aGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGTCGCTCCCGCAGGCGCGGCAAAAAAGGGGATTAAAAGCGCACGGCGAAAGCGGCGTGGGGCGGAGAGCATTTCGACGCAAGACGCCGCGACGCAAGTGGGGGGGAAGCGAAGGTGTTTGCTGTc                                                                                                                                                           >  2:124974/1‑250 (MQ=255)
                                                                                                 ttACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAg                                                                                                                                                         <  1:463073/250‑1 (MQ=255)
                                                                                                         gaAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGGTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGGGGATGGGACGCAAATGTCTTTGCTGTCAGTACCag                                                                                                                                                   >  1:391018/1‑248 (MQ=255)
                                                                                                            aaTGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTTGCAGGCGTGCCCAAAGAGGGTATTAAGCGCGCACGGCACAAGCCGCGGTCGCTGGGAAGCATTTTGCCGCACGCCGCCGCCACGCCAGTGGATGGGACGCCAATGTGTTTGCGGTCAGGACCccaaga                                                                                                                                               >  2:682531/1‑249 (MQ=255)
                                                                                                                   ggCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTAAGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGc                                                                                                                                       >  1:98350/1‑250 (MQ=255)
                                                                                                                       tCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGGACGCCGCCGCTACGCAAGTGGATGGTACGCCAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGc                                                                                                                                   >  1:223012/1‑250 (MQ=255)
                                                                                                                                                     aCGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGa                                                                                                     <  1:389649/250‑1 (MQ=255)
                                                                                                                                                       ggCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGGCGGCGTGCCAAAAAAGGTTATTAAGCGCGCACGGCGAAAACTGCGTGAGCTGGAAAGGATTTCGCCGAACGCCGCCGCTACGCCAGTGGGTGGGAAGCCAATGTCTTTTCTGTCCGGACCCAGAGAAACGTCTCCTGCGGGCGAAGCTCTGGAAAAACTTGGTCCGGGtt                                                                                                   >  2:676830/1‑250 (MQ=255)
                                                                                                                                                        gCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCCAAAGAGGGTATTTAGCGCGCCCGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGCAGCTCTGGAAAATCTTGATCCGGAtt                                                                                                   >  1:630948/1‑249 (MQ=255)
                                                                                                                                                             acCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCCAAAGAGGGTATTTAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGCACGCCGCCGCCACGCCAGGGGGTGGTACGCCAAAGTCTTTGGTGTCAGTACCCGAAGAAACTTCCCCTGGGGGCGAAGCCCTGGTAAATCTTTGTTCGGGTtcacccc                                                                                             >  2:417718/1‑247 (MQ=255)
                                                                                                                                                                 ttGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCAcccc                                                                                         <  2:280107/250‑1 (MQ=255)
                                                                                                                                                                   gCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCcg                                                                                        <  2:670397/249‑1 (MQ=255)
                                                                                                                                                                     cTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGGGCCAAAAGAGGTTATTAAGCGCGCCCGGCAAAAGCTGCGTGAGCTGGGAAGCATTTCGCCGAACGCCGCCGCTACGCCAGGGGATGGTAAGCAAAAGTCTTTGCTGTCAGTACCAGAAAAAACTTCGCCTTCGGGTGGAGCTCTGGAAAAACTTTATCCCGATTCCATCACCCCCCGt                                                                                     >  2:437662/1‑250 (MQ=255)
                                                                                                                                                                         aTGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGGTGCAGCTCTGGCAGGGGTGGCAAAAGAGGGTATTAAACGCGCACGGCAAAAGCTGCGTGAGGTGGAAAGCATTTCGCCGAACGCCGCCCCTACGCAAGTGGGTGGGAAGCAAATGTCTTTGCTGTCAGTACCCGAAGAAAATTCGCCCGCGGGCGGAACTCTGGAAAATCTTGATCCGGGTTCACTCACCCCCCGGCAgg                                                                                 >  2:541378/1‑250 (MQ=255)
                                                                                                                                                                          tGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGGGCCAAAAGAGGTTATTAAGCGCGCCCGGCAAAAGCTGCGTGAGCTGGAAAGCATTTTGCCGAACGCCGCCGCTACGCAAGGGGATGGGACGCAAATTTCTTTGCTGTCAGTACCCGAAAAAACTTTGCCCTCGGTCGGAGCTCTGGAAAATCTTGATCCGGGTTCCCTTCCCCCCCGTCAggg                                                                                >  2:191075/1‑249 (MQ=255)
                                                                                                                                                                           gCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGGTATTAAAAGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGAATTTCGCCGAACGCCGCCGCTACGCAAGTGGGTGGTACGCAAAAGTCTTTGCTGGCAGTACCAGAAGAAAATTCGCCCGCGGTCGAAGCTCTGGGAAATCTTGATCCGGATTCAATCACCCCCCGGCCGgcg                                                                               >  2:394542/1‑250 (MQ=255)
                                                                                                                                                                                     cAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTgg                                                                      <  1:483982/249‑1 (MQ=255)
                                                                                                                                                                                      aGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGGGCCAAAAGAGGGTATTAAACGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCCAGTGGATGGGAAGCAAAAGTCTTTTCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGGAAATCTTTATCCGGGTTCACTCACCCCGCGTCAGGCGCTGGAgggggt                                                                    >  2:413069/1‑248 (MQ=255)
                                                                                                                                                                                       ggATGGCGGGGCGAGCAAAAGCTACGGCCTGGCGGTGGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACGTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGAtt                                                                   <  2:19232/250‑1 (MQ=255)
                                                                                                                                                                                                 gcgAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGa                                                         <  1:28158/250‑1 (MQ=255)
                                                                                                                                                                                                            cTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGGTATTAAACGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGGGGGTGGGAAGCAAATGTCTTTGCTGTCAGTAACAGAAGAAACTTCGCCCGCGGTCGAAGCTCTGGGAAATCTTGATCCGGGTTCACTCACCCCCCGTCAGGCGCTGGGGGGGGTTTATCGCTTGAAGAGACTgggg                                              >  2:161017/1‑248 (MQ=255)
                                                                                                                                                                                                                       cGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAAtt                                   <  2:256128/250‑1 (MQ=255)
                                                                                                                                                                                                                           tGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTccc                                >  2:18002/1‑249 (MQ=255)
                                                                                                                                                                                                                               gCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAg                           <  2:781389/250‑1 (MQ=255)
                                                                                                                                                                                                                                 tctGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAGTc                         >  2:731683/1‑250 (MQ=255)
                                                                                                                                                                                                                                  ctGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAGTCt                        >  2:642942/1‑250 (MQ=255)
                                                                                                                                                                                                                                      cAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAGTCTTTTg                    <  2:409869/250‑1 (MQ=255)
                                                                                                                                                                                                                                        ggCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAGTCTTTTGCt                  <  1:74925/250‑1 (MQ=255)
                                                                                                                                                                                                                                          cGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAGTCTTTTGCTAt                >  2:503660/1‑250 (MQ=255)
                                                                                                                                                                                                                                                    gagGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAGTCTTTTGCTATCGGGAATAtt      <  2:559379/250‑1 (MQ=255)
                                                                                                                                                                                                                                                        ttattaAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAGTCTTTTGCTATCGGGAATATTAACg  <  1:692939/250‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         
CGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAGTCTTTTGCTATCGGGAATATTAACG  >  NZ_CP009273/2852557‑2853054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑