Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,171,1070CA34.8% 123.9 / 25.5 69E55* (GAG→TAG) nudFADP‑ribose diphosphatase
Reads supporting (aligned to +/- strand):  ref base C (37/8);  new base A (1/23);  total (38/31)
Fisher's exact test for biased strand distribution p-value = 1.31e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCCCCCCGGGCTTGCCAGG‑AAACTTAACACCGGTTTGGTCCGTTTGACTATCA‑GTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTA  >  NZ_CP009273/3170862‑3171348
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 
gCCCCCCGGGCTTGCCAGG‑AAACTTAACACCGGTTTGGTCCGTTTGACTATCA‑GTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCaaa                                                                                                                                                                                                                                                >  2:765755/1‑249 (MQ=255)
  cccccGGGCCTTCCAAAAAAACTCAACCCCGTTTTGACCGTTTTGAATATCA‑GTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCaaaaa                                                                                                                                                                                                                                              <  1:758889/250‑1 (MQ=255)
   ccccGGTCTTGCCAGG‑AATCTAAACCCCGGTTTGGCCCGTTGGACTATCA‑GTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAAtt                                                                                                                                                                                                                                            <  2:72518/250‑1 (MQ=255)
             gCCAGG‑AAACTTAACACCGGTTTGGTCCGTTTGACTATCA‑GTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCa                                                                                                                                                                                                                                  >  1:414667/1‑250 (MQ=255)
                     aaCTTAACACCGGTTTGGTCCGTTTGACTATCA‑GTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCAt                                                                                                                                                                                                                            >  1:701925/1‑249 (MQ=255)
                       cTTAACACCGGTTTGGTCCGTTTGACTATCA‑GTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGAc                                                                                                                                                                                                                         >  2:528313/1‑250 (MQ=255)
                       cTTAACACCGGTTTGGTCCGTTTGACTATCA‑GTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGAc                                                                                                                                                                                                                         >  1:764462/1‑250 (MQ=255)
                            cacCGGTTTGGTCCGTTTGACTATCA‑GTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATAACTCAtt                                                                                                                                                                                                                    >  2:734764/1‑250 (MQ=255)
                               cGGTTTGGCCCGTTTTGATTTCACGCCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCCCCCTCTTCAATCACCCCGGCACCCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTAAATCAGCACAACTTCACCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTg                                                                                                                                                                                                                  <  1:197334/250‑1 (MQ=255)
                                  tttGGTCCGTTTGACTATCA‑GTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCg                                                                                                                                                                                                              >  2:594341/1‑250 (MQ=255)
                                       tCCGTTTGACTATCA‑GTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGaa                                                                                                                                                                                                         >  2:41775/1‑250 (MQ=255)
                                             tGACTATCA‑GTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGAc                                                                                                                                                                                                    >  2:645988/1‑249 (MQ=255)
                                              gACTATCA‑GTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGa                                                                                                                                                                                                  >  2:198064/1‑250 (MQ=255)
                                                 ctaccgtccccGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCACCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACGGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATg                                                                                                                                                                                                <  1:469364/242‑1 (MQ=255)
                                                  aTCA‑GTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGAc                                                                                                                                                                                              >  1:718503/1‑250 (MQ=255)
                                                    ccgccccccccTCTTCAATCGCTCCGCGACGGGCAACATCTTCCACACTTTCCCCCTCTTCAACCATCCCGGCAACCACCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACGGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGAc                                                                                                                                                                                              <  1:607302/244‑1 (MQ=255)
                                                    catcccccGCCTCTCCAACCGCTCCGCGCCGGCCACCATCTCCCACACTTTCCCCCTCTCCAATCACCCCGGCACCCACCTCCAGTAGCCAGGGGGTTTCGCTGGTGTCGTCCGCGGCAACCCGAATCTGCTCAACCAGCACACCTTCATCACGCACGGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACg                                                                                                                                                                                             <  2:215115/245‑1 (MQ=255)
                                                           cGCCTCTCCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCCCCCTCTTCAACCATCCCGGCACCCATCCCCAGTAGCCAGGGGGTTTCGCGGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCta                                                                                                                                                                                      <  1:366014/250‑1 (MQ=255)
                                                                   cAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCCCCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAGGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATc                                                                                                                                                                              <  1:111062/250‑1 (MQ=255)
                                                                    aatcgatcccggACGGACAACATCTCCCCCCCTTTCCCCCTCTCCAAACACCCCGCCAACCTTCCCCAGAACCCAAGGGGTTCCGCGGGTGTCGCACGCGGCATTCCGAATCTGCCCAATCACCACAACTCCCCCACGCGTTGGGTCAAGGGGTAGCAAGACTGCGGCGTGACCGCACTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCt                                                                                                                                                                             <  2:73197/239‑1 (MQ=255)
                                                                           tCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaa                                                                                                                                                                       >  2:713875/1‑249 (MQ=255)
                                                                               gACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaaa                                                                                                                                                                    >  1:462889/1‑248 (MQ=255)
                                                                                      aaCATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGa                                                                                                                                                           >  2:628454/1‑250 (MQ=255)
                                                                                        cATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGAt                                                                                                                                                          >  2:152509/1‑249 (MQ=255)
                                                                                         aTCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATa                                                                                                                                                         >  2:112399/1‑249 (MQ=255)
                                                                                              ccACCCTTCCCCCCTCTCCACTCACCCCGGCACCCCCCCCCAGTAGCAAGGGGGTTTCGCGGGTGTCGTACGCGACAACCCGAATCTGCTCAACCAGCACAACTCCACCCCGCACTGGGAAAAAGGGTAGAAAGACTGCGGCGTGCCCGCGCAAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAgt                                                                                                                                                     <  2:213763/248‑1 (MQ=255)
                                                                                               cacaCTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGtt                                                                                                                                                  >  2:510809/1‑250 (MQ=255)
                                                                                                   cTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTtctc                                                                                                                                              >  1:664596/1‑250 (MQ=255)
                                                                                                      tCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTg                                                                                                                                           >  1:358387/1‑250 (MQ=255)
                                                                                                           cgctcaaaccaccccGGCACCCCACTCCAGTAGCCAAGGGTTTTCGCTGGTGTCGACCGCGGAAACCCGAATCTGCTAAACCAGCACAACTTCATCACGCACTGGGTCAAAGGGGAGCAAGACTGCGGCGTGACCGCGCAAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaata                                                                                                                                      <  2:508058/238‑1 (MQ=255)
                                                                                                             ctTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATACAGTGTTTCTCGTGCaatact                                                                                                                                    >  2:569356/1‑248 (MQ=255)
                                                                                                                  atcatcCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTa                                                                                                                               >  2:161027/1‑250 (MQ=255)
                                                                                                                     atcCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACAt                                                                                                                            >  1:387140/1‑250 (MQ=255)
                                                                                                                      tcCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATc                                                                                                                           >  1:305029/1‑250 (MQ=255)
                                                                                                                      tcCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATc                                                                                                                           >  2:34649/1‑250 (MQ=255)
                                                                                                                        ccGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGt                                                                                                                         >  2:325794/1‑250 (MQ=255)
                                                                                                                             acccATCCCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAACCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTg                                                                                                                    <  1:158406/248‑1 (MQ=255)
                                                                                                                             acccATCCCCAGTAGCAAAGGGTTTGCGCTGGTGTCGAACGCGGCAATCCGAATCTGCTAAAGCAGCACAACTTCACCCCGCACGGGGTCAAGGGGTAGAAAGGCTGCGGCGTGCCCGCGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTg                                                                                                                    <  2:403406/248‑1 (MQ=255)
                                                                                                                                              aaGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAg                                                                                                   >  2:377847/1‑250 (MQ=255)
                                                                                                                                                 gggTTACACTGGTGTCGCGCCGGAAAATCAAAATCTGCACAAACAGCGCAATCTCACCACAAACAGTGACAAAGGGTAACAAGTGTGCGGCGGGCCCGCGTTAAAAATTTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGtt                                                                                                <  2:451081/250‑1 (MQ=255)
                                                                                                                                                            gtgtCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTCCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCtt                                                                                       <  1:280421/248‑1 (MQ=255)
                                                                                                                                                              gtCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAg                                                                                    >  1:512836/1‑249 (MQ=255)
                                                                                                                                                               tCGTCCGCGGAAACCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGCCTGCGGCGTGACCGCGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGc                                                                                   <  2:232362/249‑1 (MQ=255)
                                                                                                                                                                 gtccccgGCACCCCAAATCTGCAAAAACACCCCACCTTCCTCACGCCCGGGCTCAAAGGGTAGCAAGCCGGCGCCGTGACCCCACTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCa                                                                                  <  2:597966/243‑1 (MQ=255)
                                                                                                                                                                 gTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCAtt                                                                                >  2:117681/1‑250 (MQ=255)
                                                                                                                                                                   ccgcgGCAATCCGAATCTCCTCAATCAGCACAACTTCATCCCGCACTGGTTCAAAGGGTAGCAAGACGGCGGCGTGACCGAGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGt                                                                              <  2:396180/249‑1 (MQ=255)
                                                                                                                                                                     gcgGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGtt                                                                             >  1:256554/1‑249 (MQ=255)
                                                                                                                                                                     cgggCAACCCGAACTTCCCCAACCACCACAACTCCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTa                                                                            <  2:563268/248‑1 (MQ=255)
                                                                                                                                                                        gCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATg                                                                         >  1:439138/1‑250 (MQ=255)
                                                                                                                                                                         cAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGt                                                                        >  1:611419/1‑250 (MQ=255)
                                                                                                                                                                            tCCGAATCTTCACAACCAGCACACGTGCAGCACACACGGGGTCAAAGGGTAGCAGGACTGAGGCATGCCCGCGCGAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCt                                                                     <  1:550191/250‑1 (MQ=255)
                                                                                                                                                                            gcagAATCGGCCCAACCAGCACAACTCCACCCCGAGCTGGGTCAAAGGGAAGCAAGCCGGCGGCGTGCCCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCt                                                                     <  2:787110/247‑1 (MQ=255)
                                                                                                                                                                                     gccaaaTCAGCACAACTCCACCCCGCACTGGGACAAAGGGTAGCAAGCCTGCGGCGTGCCCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAAt                                                            <  2:512836/246‑1 (MQ=255)
                                                                                                                                                                                        cAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGt                                                         >  1:328121/1‑250 (MQ=255)
                                                                                                                                                                                             aGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCCTTTGCCCGTTGCATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGAGTTTCTCCTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAAt                                                     >  1:462495/1‑249 (MQ=255)
                                                                                                                                                                                             aGCACAACTCCAGCACGCACGGGGACAAAGGGTAACAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAAtt                                                    <  2:673496/250‑1 (MQ=255)
                                                                                                                                                                                              gCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTa                                                   <  1:112399/250‑1 (MQ=255)
                                                                                                                                                                                               tacaACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGAAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATTGTGAATTa                                                   >  2:66935/2‑249 (MQ=255)
                                                                                                                                                                                                acaACTTCACCACGCTCTGGGCCAAAGGGTAGCAAGCCTGCGGGTTGACCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTaaa                                                 <  2:338345/250‑1 (MQ=255)
                                                                                                                                                                                                 caACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAAt                                                >  2:312200/1‑250 (MQ=255)
                                                                                                                                                                                                  aaCTTCATCACGCACTGGGTCAAAGGGTAGAAAGACTGCGGCGTGACCGCTCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAAtg                                               <  1:683670/250‑1 (MQ=255)
                                                                                                                                                                                                         cccgccatggggACAAGGGTTAGCAAGACTGCGGTGGAACCCCCCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAAttt                                        <  2:291956/241‑1 (MQ=255)
                                                                                                                                                                                                          caCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAAttt                                        >  2:389309/1‑249 (MQ=255)
                                                                                                                                                                                                            cccatcgggTAAATGGGTAGAAAAGCTGCGGCGTGACCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAg                                     <  2:149850/244‑1 (MQ=255)
                                                                                                                                                                                                              cACTGGGTCAAGGGGTAGAAAGACTGCGGCGGGACCGCCCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCg                                   <  2:331453/250‑1 (MQ=255)
                                                                                                                                                                                                              cACTGGATCAAAGGGTAGCAAGCCTGCGGCGTGCCCGCGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCg                                   <  1:256243/250‑1 (MQ=255)
                                                                                                                                                                                                                 tGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAATTGTGAATTTCAGCGACg                                >  1:756341/1‑250 (MQ=255)
                                                                                                                                                                                                                       aaaGGTAAGCAAGACTGCGGCGTGACCGCCCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGAc                          <  1:733146/250‑1 (MQ=255)
                                                                                                                                                                                                                                             tGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATgtgt    >  2:86012/1‑250 (MQ=255)
                                                                                                                                                                                                                                               accccccacAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTa  <  2:786628/242‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 
GCCCCCCGGGCTTGCCAGG‑AAACTTAACACCGGTTTGGTCCGTTTGACTATCA‑GTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTA  >  NZ_CP009273/3170862‑3171348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑