Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,492,581	0	A	C	35.3%	131.0 / 12.5	68	L229R (CTG→CGG)	insG	IS4‑like element IS4 family transposase
Reads supporting (aligned to +/- strand):  ref base A (6/38);  new base C (24/0);  total (30/38)
Fisher's exact test for biased strand distribution p-value = 3.95e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCCCCCATTTCTCCTCCGGGGAAGCGCATGGCGTCCGTCATCGACGTCAGCAGATGGCAGACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCC  >  NZ_CP009273/4492335‑4492829                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                           tCCCCCATTTCTCCTCCGGGGAAGCGCATGGCGTCCGTCATCGACGTCAGCAGATGGCAGACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGc                                                                                                                                                                                                                                                         <  1:175698/250‑1 (MQ=255)  cccccATTTCTCCTCCGGGGAAGCGCATGGCGTCCGTCATCGACGTCAGCAGATGGCAGACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCt                                                                                                                                                                                                                                                        <  2:257833/250‑1 (MQ=255)      cATTCCCCCCCCGGGGAAGCGCATGGCGTCCGTCATCGCCGTCAGCAGATGGCAGACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAg                                                                                                                                                                                                                                                    <  2:177531/250‑1 (MQ=255)              ctccGGGGAAGCGCATGGCGTCCGTCATCGACGTCAGCAGATGGCAGACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑tt                                                                                                                                                                                                                                              <  2:708523/247‑1 (MQ=255)                ccGGGGAAGCGCATGGCGTCCGTCATCGACGTCAGCAGATGGCAGACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACa                                                                                                                                                                                                                                         <  1:257551/250‑1 (MQ=255)                  ggggAAGCGCATGGCGTCCGTCATCGACGTCAGCAGATGGCAGACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGt                                                                                                                                                                                                                                       <  1:227304/250‑1 (MQ=255)                           cATGGCGTCCGTCATCGACGTCAGCAGATGGCAGACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGGTTTTTCCCCCCCGGCTCCAGGGACTTTAACAG‑CCCAGTggg                                                                                                                                                                                                                              >  2:250931/1‑248 (MQ=255)                            aTGGCGTCCGTCATCGACGTCAGCAGATGGCAGACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTTCCGCCCGGCCCCCGGCC‑CTTAACAGTcccccggggt                                                                                                                                                                                                                             >  2:359738/1‑243 (MQ=255)                              ggCGTCCGTCATCGACGTCAGCAGATGGCAGACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCCCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCCGGCTCCAGGCA‑TTTAACAGTCCCCGGGAGgaa                                                                                                                                                                                                                           >  1:23839/1‑250 (MQ=255)                                cgtccgtcATCGACGTCAGCAGATGGCAGACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGtaata                                                                                                                                                                                                                         <  2:105714/250‑1 (MQ=255)                                       cATCGACGTCAGCAGATGGCAGACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTa                                                                                                                                                                                                                  <  2:46883/250‑1 (MQ=255)                                         tCGACGTCAGCAGATGGCAGACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCC‑TTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCCCCAGGCTCCCGGCA‑TTTAACAGTCCCCGTGAGTAATAACCttttttc                                                                                                                                                                                                               >  1:107614/1‑246 (MQ=255)                                                   cagATGGCAGACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGAGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTc                                                                                                                                                                                                      <  2:558424/250‑1 (MQ=255)                                                    agATGGCAGACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCa                                                                                                                                                                                                     <  1:443987/250‑1 (MQ=255)                                                    agATGGCAGACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCa                                                                                                                                                                                                     <  1:474448/250‑1 (MQ=255)                                                            gACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTa                                                                                                                                                                                             <  2:293214/250‑1 (MQ=255)                                                                  tCCTTTGCGCGTCCCGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGcc                                                                                                                                                                                        <  1:156913/249‑1 (MQ=255)                                                                   ccTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTTTCCCCCCAGGCTCCCGGCA‑TTTTACAGTCCCAGTGAGTGATAAACCTTATTCATTAACGTCAGAGTGTTATCGCCgg                                                                                                                                                                                      >  1:697749/1‑250 (MQ=255)                                                                    cTTTGCGCGTCACGATCAGCAGGCGTGCAGTCTCTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCgg                                                                                                                                                                                      <  1:565391/249‑1 (MQ=255)                                                                       agcgcGTCGCGTACACCAGGCGGGCAGGCACTTCATTTCCCAGTCCCGGCCCCTTTTATCGTGCCTGCGGGCTGGTGTTCAGCTTCACCAGATGATCGCCTTTACCCAGAGTTCTGATCTCTTCAAATTGCGCTCCCTTTCTGAAAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGtt                                                                                                                                                                                    <  2:612980/247‑1 (MQ=255)                                                                             tCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCtat                                                                                                                                                                            <  2:359300/250‑1 (MQ=255)                                                                               aCGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCtata                                                                                                                                                                           <  1:624776/249‑1 (MQ=255)                                                                                      gcagGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑c                                                                                                                                                                  <  1:780560/250‑1 (MQ=255)                                                                                        agGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGATTGTTCTATAAGTTGCT‑CAg                                                                                                                                                                <  1:774314/250‑1 (MQ=255)                                                                                            gggCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCCCCCGGCCCCCGGCC‑TTTTACAGTCCCCGTGAGGAATAACCTTTATCCCTTAACGCCAGAGTGTTATTGCCCGGTTGTTCTTTTAGTTTCT‑CAGCAAg                                                                                                                                                            >  1:62947/1‑250 (MQ=255)                                                                                                aGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTTTCCCGCCCCGCTCCCGGCC‑TTTTACAGGCCCAGTGAGTAATAACCTTTTTCCATTTACGTCAGAGTGTTATTGCCGGCTTGTTCTTTTAGTTGTT‑CAGCAAGCCCa                                                                                                                                                        >  2:125137/1‑250 (MQ=255)                                                                                                 gTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCAt                                                                                                                                                       <  1:305970/250‑1 (MQ=255)                                                                                                     cTTCATTTCCCAGTCCCGGCCCCTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTc                                                                                                                                                   <  2:783113/250‑1 (MQ=255)                                                                                                     cTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTc                                                                                                                                                   <  1:390155/250‑1 (MQ=255)                                                                                                      ttCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTTTCCCGCCCGGCTCCCGGCC‑TTTTACAATCCCCGTGGGTAATAACCTTTTTCCATTTAACTCAGAGTGTTTTCTCCCGGTTTTTCTATAAATTTCT‑CCGCCAGCGCATTTTct                                                                                                                                                  >  2:152587/1‑249 (MQ=255)                                                                                                        cATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCg                                                                                                                                                  <  2:23979/248‑1 (MQ=255)                                                                                                        cATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCCCCCGGCTCCCGGCA‑TTTTACAGTCCCCGTGAGGAATAAACTTTATTCCTTAAAGTCCGGGGGTTTTTGCCGGGTTGTTCTTTAAGTTGCT‑CAACCAGCTCAttttttct                                                                                                                                                >  2:296046/1‑246 (MQ=255)                                                                                                         aTTTCCCGGTCCCGGCCACTTTTTTCGTGCTTGCGGGCTGGTTTTCAGCTTCCCCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTGTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCt                                                                                                                                                <  2:376391/249‑1 (MQ=255)                                                                                                           ttCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCCGGCTCCCGGCA‑TTTAACAGTCCCAGTGAGGAATAAACTTTATCCATTAACGTCAGAGGGTTTTCGCCGGTTTTTTCTTTAAGTTGCT‑CAGCAAGCCCCTTTTTGCTGtt                                                                                                                                             >  1:462229/1‑250 (MQ=255)                                                                                                               cAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCCGGCTCCCGGCA‑TTTAAAAGTCCCCGTGGGGAATAAACCTTTTCCATTAACGTCAGAGGGTTTTTGCCGGGTTTTTTTTTAAATTGCT‑CAGCAAACTCATTTTTGCTGttcttt                                                                                                                                         >  1:365905/1‑249 (MQ=255)                                                                                                                 gTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCCGGCTCCAGGCG‑TTTTACAGTCCCAGTGAGGAATAAACCTTTTCCATTTACGTCAGAGTGTTATCGCCGGTTTGTTCTTTAAATTGCT‑CAGCCAGCTCCTTTTTGCTGttcttcct                                                                                                                                       >  2:383812/1‑248 (MQ=255)                                                                                                                           cTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAg                                                                                                                             <  2:174608/250‑1 (MQ=255)                                                                                                                               tttCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGGTCTCCCCCCCAGCTCCAGGCA‑TTTTACAAGCCCCGGGAGTAATAAACCTTATCCATTAACGTCAGAGGGGTTTTGCCGGGTTTTTTTTTAAATTGCTCCAGAAAGC‑CCTTTTTTCTGTTTTTCATCAGGCCgcaatgcg                                                                                                                         >  2:349403/1‑243 (MQ=255)                                                                                                                                ttCTTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTg                                                                                                                         <  2:448599/249‑1 (MQ=255)                                                                                                                                   gTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCCGGCTCCAGGCA‑TTTAACAGTCCCCGTGAGGAATAAACTTTATCCATTAACGTCAGAGTGTTATCGCCGGGTTGTTCTTTAAGTTGCT‑CCGCAAGCTCCTTTTCGCTGTTTTTCCTCGTGCCGAAGGCTGccac                                                                                                                     >  2:310035/1‑247 (MQ=255)                                                                                                                                     gCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTTTCCCGCCCCGCTCCAGGCC‑TTTTAAAGTCCCCGTGAGTAATAAACTTTATTCCTTAACGTCAGAGGTTTATCCACGGTTTGTTCTTTTAATTGCT‑CCGCAAACTCATTTTCGCTGTTCTTCATCGTTCCGAAAGCTGCAGCCg                                                                                                                   >  1:705875/1‑250 (MQ=255)                                                                                                                                          cGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTcagc                                                                                                              <  1:383812/250‑1 (MQ=255)                                                                                                                                                    ttCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTTCCGCCCGGCTCCAGGCA‑TTTAACAATCCCCGTGAGTAATAAACCTTTTCCATTAACCTCAGAGGGGTATCGCCGGGTTGTTTTTTTAGTTTCT‑CAGCAAGCACATTTTCGCTGTTTTTCATTGTGCCCACGGCTGCAGCCGTCAGCAGATGGCTgg                                                                                                    >  2:57432/1‑250 (MQ=255)                                                                                                                                                             aCCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCa                                                                                           <  2:111294/250‑1 (MQ=255)                                                                                                                                                               cAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTCTTTTCCCGCCAGGCTCCCGGGA‑ATTAAAAGTCCCAGTGAGGAATAAACTTTTTCCATTAAAATCAGAGGGTTATAGCCCGTTTTTTCTATTAACTGCT‑CCGCAAGATCATTTTTGCTGTTCTTCTTCGTGCCGAAAGCTGCAGCCCCCAGAAAAGTGCTGGTCAGTTTCacc                                                                                         >  2:573108/1‑248 (MQ=255)                                                                                                                                                                         ccTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCCGGCTCCAGGCA‑TTTTACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGGTTGTTCTTTAAATTGCT‑CAACAAGCCCATTTTTGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCCGTTTCATCTGGCAGAccc                                                                               >  2:543051/1‑249 (MQ=255)                                                                                                                                                                             tACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCAtt                                                                             <  1:607295/248‑1 (MQ=255)                                                                                                                                                                               cccAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTTTCCCCCCCGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGGAATAAACCTTATCCATTAACGTCAGAGGGTTATCGCCGGTTTGTTTTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCGGCCACCGCCAGCAGATGGCTGGTCAGTTTCCTCTGGCAGACCAttttta                                                                         >  2:642664/1‑248 (MQ=255)                                                                                                                                                                               cccAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTg                                                                          <  2:687898/249‑1 (MQ=255)                                                                                                                                                                                 cAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGAcc                                                                       <  1:31363/250‑1 (MQ=255)                                                                                                                                                                                  atgtttCAGATCTCTTCATATTGCGCTCCCGTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGACCt                                                                      <  2:740417/247‑1 (MQ=255)                                                                                                                                                                                  aggtttCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGACCt                                                                      <  2:187428/247‑1 (MQ=255)                                                                                                                                                                                      ttCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTTTCCCCCCCGGCTCCAGGCA‑TTTAACAGTCCCCGTGAGTAATAACCTTTTTCCATTAAAGTCAGAGTGTTATCGCCGGGTTGTTTTATAAGTTGCT‑CAGCCAGCTCATTTTTGCTGTTTTTTATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCCTCTGGCAGACCATTTTTACCTGCgg                                                                  >  1:101685/1‑250 (MQ=255)                                                                                                                                                                                          gATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTTTTCCGCCCGGCCCCCGGCA‑TTTAACAGTCCCCGTGAGGAATAAACTTTTTCCATTAAAGTCAGAGTGGTATTGCCGGGTTGTTTTTTTAGTTTCT‑CAGGAAGCTCATTTTTTCTGTTCTTCATCGTGCCGCAAGCTGCAGCCGTCACCAGATGGCTGGGCCGGTCCATCTGGCAGCACCTTTTTACCTGCCGGTag                                                              >  2:568074/1‑250 (MQ=255)                                                                                                                                                                                              tctTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTTTTCCCCCCCGCTCCCCGCA‑TTTAAAAATCCCCGTGAGGAATAACCTTTTTTCCTTATCGTCCGAGGGTTATTGCCCGTTTTTTCTTTAAATTTTT‑CCGCCAGCTCATTTTTTCTGGTCTTCATTCTGCCGAAGGCCGACGCCGTCAGCAGTTGGCTGGTACGTTGCCTCCTGCAGGACATTTTTGCCTGTggggtggggg                                                          >  2:432629/1‑243 (MQ=255)                                                                                                                                                                                              tctTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTTTCCCGCCCCGCTCCCGGCA‑TTTAACAGTCCCAGTGAGGAATAACCTTTATCCCTTAACGTCCGAGTGTTATCGCCGGTTTGTTTTATAAGTTTCT‑CAGCAAACTCATTTTTGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCCTCTTGCCGACCATTTTTACCTTCGGGTGAAgcg                                                          >  2:396214/1‑250 (MQ=255)                                                                                                                                                                                                     atTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCCGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAAACTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCCGCAGATGGCTGGTCAGTTCCATCTGGCAGACCCTTTTGACCTGCGGGTAGAGCGCCGGGtt                                                   >  1:482988/1‑250 (MQ=255)                                                                                                                                                                                                     atTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGGGTTTTTCCGCCCGGCTCCCGGCA‑TTTTAAAGTCCCCGGGAGTAATAACCTTTATCCATTAACGTCAGAGGGTTTTCGCCGGGTTTTTCTTTAAGTTGGT‑CAGCAAGCTCATTTTTGCTGTTTTTCATCGTGCCGCAAGCTGCAGCCGTCAGCAGATGGGTGGGTAGTTTCATCTTGCAGACCCCTTTTACCTGCGGGGAGAGCGCCCGGtt                                                   >  2:614318/1‑250 (MQ=255)                                                                                                                                                                                                      ttGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGACCTGCGGGTAGAGCGCCGGGtt                                                   <  1:731972/249‑1 (MQ=255)                                                                                                                                                                                                        gcgcTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCCGGCTCCCGGCA‑TTTAACAGTCCCAGTGGGGAATAACCTTTATCCATTAACGTCAGAGTGGTATCGCCGGTTTGTTTTTTAAGTTGCT‑CAGCAAGCCCATTTTTGCTGTTCTTCATCGTGCCGCAGGCTGCAGCCCTCCGCAGATGGCTGGTCAGTTTCCTCTGGCCGACCCTTTTGGCCCGCGGGGAGAGCGCCGGGTTccc                                                >  2:22287/1‑250 (MQ=255)                                                                                                                                                                                                         cgcTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTTTCCCGCCAAGCTCCAGGCA‑TTTAACAATCCCCGTGAGTAATAACCTTTATCCATTAACGTCCGAGTGTTATTGCCGGGTTGTTTTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGCAGGCTGCCGCCGTCCGCAGATGGCTGGTCAGTTCCCTCTGGCCGACCATTTTGGCCCTCGGGTAGAGCGCCGGGTTcccc                                               >  1:737470/1‑249 (MQ=255)                                                                                                                                                                                                         cgcTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCg                                               <  1:311399/250‑1 (MQ=255)                                                                                                                                                                                                         cgcTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCCCCAGGCTCCAGGGA‑TTTTACACTCCCAGTGAGTAATAACCTTTATCCATTAAAGTCCGAGTGTTATCGCCGGTTTGTTCTTTAAGTTGCC‑CAGCAAGCTCATTTTTGCTGTTCTTTTTCCTGCCGAAGGCTGCAGCCGCCCGCAGAAGGCTGGTCAGTTTCATCTGGCACACCATTTTGACCCGCGGGGAGAGCGCCGGGTTCCCg                                               >  1:473789/1‑250 (MQ=255)                                                                                                                                                                                                          gcTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCgg                                              <  2:19077/250‑1 (MQ=255)                                                                                                                                                                                                                       gagGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCg                                 <  2:163295/250‑1 (MQ=255)                                                                                                                                                                                                                              catcCAGTGGCGGTGTTCTCCCCCCCGGCCCCCGGCA‑TTTTACAGTCCCCGTGAGTAATAACCTTTATCCCTTTACGTCAGAGTGTTATCGCCCGTTTTTTCTATAAGTTTCT‑CCGCAAGCTCCTTTTTTCTGTTCTTCCTCGTTCCGAAGGCTTCCGCCCTCCGCCGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTTACCTGCGGGTAGAGCGCCCGGGTCCCGGCCTGTGTCTGGCGGGGGGAg                          >  2:360077/1‑250 (MQ=255)                                                                                                                                                                                                                                                tcCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGCAGGCTGCATCGGTCTCTggc        >  2:313858/1‑249 (MQ=255)                                                                                                                                                                                                                                                      cAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATcc  <  2:440033/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                           TCCCCCATTTCTCCTCCGGGGAAGCGCATGGCGTCCGTCATCGACGTCAGCAGATGGCAGACTTTTCCTTTGCGCGTCACGGTCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCA‑TTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCT‑CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCC  >  NZ_CP009273/4492335‑4492829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑