Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,819,313	0	G	A	35.5%	106.8 / 23.4	62	intergenic (‑119/+84)	ves/spy	environmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy
Reads supporting (aligned to +/- strand):  ref base G (36/4);  new base A (0/22);  total (36/26)
Fisher's exact test for biased strand distribution p-value = 4.36e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGC  >  NZ_CP009273/1819065‑1819548                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              cgctggaggCAATGGTAGCAAGCCAGTAAGAATCTCGTTTAGGCGGTGGGAACGTGCAAGTTTCGCGCGTTCCACAGACAACATTT‑CGCCACAGATTCACCGACATTTTTCGCATATCAAAGTATTGCATACTCGCTCCCGGCACGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCTGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTgagaagagg                                                                                                                                                                                                                                              <  2:482458/247‑2 (MQ=255)   atggatgCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTgagaagagaa                                                                                                                                                                                                                                             >  1:316981/1‑249 (MQ=255)    tggatgCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTgagaagagaa                                                                                                                                                                                                                                             >  1:378492/1‑248 (MQ=255)       atgAAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGagaaaagaa                                                                                                                                                                                                                                        <  2:661050/250‑1 (MQ=255)        tgCAATGCTGGCTCGCCAGTAAAAGTCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGagaaaagaaa                                                                                                                                                                                                                                       <  1:488305/250‑1 (MQ=255)        tgCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGagaaaagaaa                                                                                                                                                                                                                                       <  2:52052/250‑1 (MQ=255)        tgCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGagaaaagaa                                                                                                                                                                                                                                        >  1:437495/1‑249 (MQ=255)            aTGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑ccg                                                                                                                                                                                                                                  <  2:556304/250‑1 (MQ=255)               cTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑ccgcc                                                                                                                                                                                                                                <  1:501362/249‑1 (MQ=255)                tGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGa                                                                                                                                                                                                                              >  1:628689/1‑250 (MQ=255)                           tAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCAcc                                                                                                                                                                                                                   >  2:126679/1‑250 (MQ=255)                                    cGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTg                                                                                                                                                                                                          >  1:290255/1‑250 (MQ=255)                                      tgtggcgggggggAAGGTGAAAATTCCGCGGTTTTCACC‑GCAACTTTTCCCCCACAGTTCCCCCTATTTTTTACGAATATAAAATTTTCCAATCCCCGCCCCCGGCCTGTTTTTTTTTTTTTTATCTTGCAGGTAA‑CG‑GTGAATGTTTAGCGGCAACTGGGTTAATTTACGTTAGTGGTGTCCGGGTTTTTTTCACAA‑CTTTTGAAAAAGAAAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGc                                                                                                                                                                                                         <  2:699785/239‑1 (MQ=255)                                       tttccgggggggAACTTGCAAATTGCGCGCGTTCCACCGGCAGCTTTC‑CCCCACAGTTTCACCGATATTTTACGCATATCAATGTATTCCATCCTCGCCCCCGGCCCTTTTTTTTATTTGTTATCGTTCAGGTAC‑CC‑GTGGATGTTTACCGGCAACTGGGGTGATTTACGTTGGTGGTGATCGGTTTTTTTTTACAC‑TTGTTTGAAAAGAAAAAAAAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCa                                                                                                                                                                                                        <  2:590558/240‑1 (MQ=255)                                        ttGCCGGTGGGACCGTGCAATTTTCGCGCTTTACACGGGCAGCATTC‑CCCCACAAATTCCCCGACTTTTTACGAATATAAAAGTTTCCCATCCTCGCTCCCGGCCTGTTTTTTTCATTTTTATCGTTCAGGTAA‑CC‑GTGGTTGTTTACCGGCAACTGGGGTTATTTACGTTAGTGGTGATCAGGTTTTTTTTACAA‑TTGTTTGAGAAGAAAAAAAAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCaa                                                                                                                                                                                                       <  1:564704/249‑1 (MQ=255)                                          gCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAgg                                                                                                                                                                                                     >  1:721158/1‑249 (MQ=255)                                            cGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg                                                                                                                                                                                                  >  2:561067/1‑250 (MQ=255)                                                gggAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGa                                                                                                                                                                                              >  2:640165/1‑250 (MQ=255)                                                     cGTGCAAATTTCGCGCGTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGAAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGa                                                                                                                                                                                         >  1:678208/1‑250 (MQ=255)                                                         cAAATTCCGGGCGTTGCCCCGGCGACATTT‑CGCCACAGATCCCCCGACTTTTAACACATATAAATGTTTTCCATCCTCCCTCCCGGCCGTTTTTTTTTATTTTTATCGTTAAGGTAA‑CC‑GTGTATGTTTACCGGCACCTGGGGTGATTTGCGTTGGGGTTAATAGGGTTTTTTTTAAAA‑TTTTGAGAAAAGAAAAAAAAAAC‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg                                                                                                                                                                                     <  2:750334/250‑1 (MQ=255)                                                              tccgcgctTTCCACCGGCAGCTTTC‑CGCCACAGATCCCCCGACATTTTCCCCATATCAAAGTTTTCCATCCTCGCCCCCGGCCCTTTCTTTTCATTTTTATCGTTCAGGTAC‑CC‑GTGGATGGTTACCGGCACCTGGGTTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACAC‑CTTTTTGAAAAGAAAAAAAAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGt                                                                                                                                                                                 <  1:307927/247‑1 (MQ=255)                                                                 gcggttttCACGGGAAGCATTA‑CGCCAAAAATCCACCAAATTTTAACGAATAAAAAAGTATTCCATACCCGCCCCCGGCCGGTTTTTTTCTTTTTTATCGTTAAGGTAA‑CG‑GGGGATGGTTAGCGGAAACGGGGGTGATTTACGTTAGGGGTGATAAGGTTTTTTTAACAA‑CTGTTGGAGAAGAAAAAAAAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTa                                                                                                                                                                             <  1:291373/245‑1 (MQ=255)                                                                  cgcgTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                                                                                                              >  1:156032/1‑248 (MQ=255)                                                                   gcgTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAg                                                                                                                                                                           >  1:323098/1‑250 (MQ=255)                                                                           ccGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCTTTAGTTTTTTgg                                                                                                                                                                   >  1:731097/1‑250 (MQ=255)                                                                                    ttt‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGGAAGaa                                                                                                                                                          >  2:580383/1‑249 (MQ=255)                                                                                         gCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt                                                                                                                                                      >  1:329962/1‑250 (MQ=255)                                                                                            ccatatcccccGCCATTTTACGCATACCAAAGTTTTCCATTCCCGCCCCCGGCCCTTTTTTTTCTTTGTTATCTTTGAGGTAA‑CT‑GTGGTTGTTTACCGGAACCTGGGTTTTTTTACGTTAGGGGTGAGCAGTTTTTTTTTACAA‑CTGTTGGAAAAGAAAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcag                                                                                                                                                   <  2:297313/241‑1 (MQ=255)                                                                                             atgATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCCACTCGGGTGTTTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CAGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCAGACTCAATTTTGTTTACCGGAAGgttatttctc                                                                                                                                                  >  2:388228/3‑246 (MQ=255)                                                                                                   ccccgtttttttACAAATATCATATTTTTCCATACTCTCTCCCGGCCCTTTTTTTTCTTTGTTTTCGTTCAGGTAA‑CG‑GGGGATGTTTATCGGCAACTGGGGTTTTTTACGTTGGGGGTGATCAGTTTTTTTTTACAA‑TTGTTGGAGAAGAAAAAAAAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGtt                                                                                                                                              <  2:273743/240‑1 (MQ=255)                                                                                                   cACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCCTGCGTTTTTTTGCTGAAAGATTTTTctcccattac                                                                                                                                            >  2:387598/1‑241 (MQ=255)                                                                                                        actttttcccaataaCAAATTATCCCATCCCCGCCCCCGCCCGTTTTTTTTCTTTGTTATCGTTCAGGTAA‑CC‑GTGGATGTTTATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCGGTTTTTTTTTACAA‑CTTTTAGAAAAGAGAAAAAAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCa                                                                                                                                       <  1:299203/239‑1 (MQ=255)                                                                                                          aTTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTGGCTGAAAGATTATTCAGCAGTTTCAGGCAtt                                                                                                                                     >  1:697417/1‑250 (MQ=255)                                                                                                            tttccccATATAAAATTTTTCCATCCCCCCCCCGGGCCTTTTTTTTTATTTTTTATCGTTAAGTTAC‑CC‑GTGGTTGTTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAC‑TTTTTTGAGAAGAAAAAAAAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAttt                                                                                                                                    <  1:581562/243‑1 (MQ=255)                                                                                                             ttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGACGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGACTTAAGTTTTTTGGTTGTAAGATTATTCAGCAGTTGCAGGCAGTTTa                                                                                                                                  >  2:635918/1‑250 (MQ=255)                                                                                                                      tCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGGAAGATTATTCAGCAGTTTCAGGCATTTTTCCTTTTGcc                                                                                                                         >  1:157518/1‑250 (MQ=255)                                                                                                                      tCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCTTTAGTTTTTTTGGTTAAAGATTTTTTACCAGTTTACGGGATTTTTTCTTTTTcc                                                                                                                         >  2:348205/1‑250 (MQ=255)                                                                                                                                ccATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGtt                                                                                                               >  1:135094/1‑250 (MQ=255)                                                                                                                                     cTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGt                                                                                                           >  1:167245/1‑249 (MQ=255)                                                                                                                                          tCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg                                                                                                     >  1:186109/1‑250 (MQ=255)                                                                                                                                          ccccGGCCTGTTTTTTTATTTGTTATCTGTGA‑GTAA‑CCGGTGGTTGGTTTTCGCCAACTGGGGTGATTTAGTTTAGGGGTGATCGGGTTTTTTTAAAAA‑CTGTTGGAAAAGAAAAAAAAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg                                                                                                     <  2:378492/249‑1 (MQ=255)                                                                                                                                           cccGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTTGCTGGAAGATTATTTAGCAGTTGCCGGCCTTTTTCCTTTTGCCGCTTGACGTTCTGTTAGACGc                                                                                                    >  2:247874/1‑250 (MQ=255)                                                                                                                                           cccGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTGGGTGAAAGATTATTTAGCAGGTGCAGGCATTTTACCTTTTGCCGCTGGACGtttcttcatcagc                                                                                                    >  2:503982/1‑239 (MQ=255)                                                                                                                                             cGGCCGTTTCTTTTCATTGTTATCGTTAAGGTAG‑CC‑GTGGTTGGTTTGCGGCAACTCGGTTGATTTACGTTAGTGGTGATCGGTCTTTTTTTACAT‑CTTTTTGAAAAGAAAAAAAAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt                                                                                                  <  2:476724/250‑1 (MQ=255)                                                                                                                                                cccTTTCTTTTATTTTTTATCGTTCAGTTAA‑CG‑GGGGTTGGTTACCGGAAACTCGGGTTATTTACGTGAGTGGTGATCGGTTTTTTTTTACAA‑CTGTTGAAAAAAAAAAAAAAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtctc                                                                                               <  2:133437/250‑1 (MQ=255)                                                                                                                                                 ccGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtctc                                                                                               >  1:457894/1‑249 (MQ=255)                                                                                                                                                     ttttttCTTTTTTATCTTTC‑GGTAATCC‑GTGGTTGTTTACCGACAACTGGGGTTATTTACGTTGGTGTTTACCGGGTTTTTTTTAAAC‑TTTTTTGAAAAGAAAAAAAAAAC‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaaa                                                                                           <  2:484300/247‑1 (MQ=255)                                                                                                                                                       ttttCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTTAGACGCTTCTCAAAAt                                                                                          >  1:257891/1‑248 (MQ=255)                                                                                                                                                        tttCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGGAAGATTATTTAGCAGTTGCAGGCCTTTTACCTTTTTCCGCTGGACGTTTTTTCAGACGCTTCTCAAAAtttg                                                                                       >  2:442934/1‑248 (MQ=255)                                                                                                                                                         ttCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGACTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGGTGAAAGATTATTTAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGGCGTTTTGTTAAAAGCTTTTCAAAATTaa                                                                                       >  2:445175/1‑248 (MQ=255)                                                                                                                                                                    tgtctaGGTAC‑CC‑GGGGTTGTTTATGGGCAATTGGGGTTATTTATTGTTGTGTTGATCGGTTTTTTTTTACAT‑CTGTTGGAAAAGAAAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGc                                                                            <  2:174176/244‑1 (MQ=255)                                                                                                                                                                     gTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAACTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCtt                                                                          >  2:120096/1‑250 (MQ=255)                                                                                                                                                                         aGGTAA‑CG‑GTGGTTGTTTGTGGGCAACGGGGTTGTTTTGATTTGGGGGTGATCGGTTTTTTTTAAAAA‑TTGTTTAAAAAAAAAAAAAAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTc                                                                      <  1:559421/250‑1 (MQ=255)                                                                                                                                                                                gcttgtggtGGTTATCGTAAACTCTGGTGATGTGAGTTAGTGGTCA‑CTTGCTGGTTTTTCAATTTGTGGAAGAGAAAAAAAGAAAC‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTGGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTc                                                                 <  2:613330/242‑1 (MQ=255)                                                                                                                                                                                    tgtggtgTTATGGGCACATCGGGTGATTTAC‑ATAGTGGTTGTGCATTTTTTTTTTCAA‑TCTTTTGAAAAGAAAAAAAAAAAGCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCg                                                               <  2:315575/242‑1 (MQ=255)                                                                                                                                                                                    tGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGc                                                             >  1:712423/1‑250 (MQ=255)                                                                                                                                                                                      gATGGTTATCGGACACTGGGTTAATTTTCGTAGGTGGTGATCAGGTTTTTTTTACAA‑CTTTTTAAGAAGAAAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGt                                                           <  1:156573/250‑1 (MQ=255)                                                                                                                                                                                           ttATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTC‑AGGa                                                     >  1:234459/1‑250 (MQ=255)                                                                                                                                                                                                                 aCGTTAGGGGTAATCGGGTTTTTTTAACAA‑CTGTTTAAAAAAAAAAAAAAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTggg                                <  1:171022/249‑1 (MQ=255)                                                                                                                                                                                                                  cGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGtt                              >  1:233771/1‑250 (MQ=255)                                                                                                                                                                                                                          ttaaccAGGTTTTTTTTACAA‑CTGTATAAGAAAAAAAAAAAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCAtgtg                      <  2:596946/245‑1 (MQ=255)                                                                                                                                                                                                                          gtaaTCAGGTTTTTTTTCCAA‑TTGTTGAAGAAAAAAAAAAAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCAtgtg                      <  1:221945/247‑1 (MQ=255)                                                                                                                                                                                                                           tgATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCTGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGACTTTAGTTTTTTTGCTGAAAGACTATTTAGCAGATGTGGGGATTTTAAATTTTTCCGCTGGACAGTCTGTCAGACACTTCTGCAAATATGCATTAAATTGCGTTTTCATATCCGGGGTCAAGGGTG‑TGTAAATTTTGGTCTTGGTTTCCATGGgc                     >  2:762962/1‑250 (MQ=255)                                                                                                                                                                                                                                aGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGACTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCCTTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTGTTCCGGCGTC‑AGGATGTTGTAAATTTTTTTTTGGGTTTCCATGTGCGCCAg                >  1:680663/1‑250 (MQ=255)                                                                                                                                                                                                                                  gCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGAATTTAGTTTTTTTGATGAAAAATAATTCAGCAGGTTGAGGAATTTTTACTTTTTCCGCTGGACAATCCTTCAGACGCCTACCAAAATTAGCATTAAATTTCTTTTTTTTTTCAGGGGCT‑AGGGTTTTTGAAATTTTTTTCTGGGTTTTCATGTTCGcccgtc              >  2:651716/1‑246 (MQ=255)                                                                                                                                                                                                                                       ttttACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCCTCTCAAAATTAGCATTTAATTTCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTTGGTTTCCATGTGCGCCAGCATGt           >  1:413390/1‑248 (MQ=255)                                                                                                                                                                                                                                           aCAA‑CTATGAGAGAAAAAAAAAAAAAG‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCt      <  2:353882/249‑1 (MQ=255)                                                                                                                                                                                                                                            cAA‑GTGTTGGAAAAGAGAAAAAAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCttt    <  1:651118/250‑1 (MQ=255)                                                                                                                                                                                                                                              a‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTgc  >  1:358884/1‑250 (MQ=255)                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              CGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTT‑CGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAA‑CC‑GTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTC‑AGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGC  >  NZ_CP009273/1819065‑1819548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑