| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 662,369 | T→G | K435N (AAA→AAC) | mrdA ← | peptidoglycan DD‑transpeptidase MrdA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 662,369 | 0 | T | G | 98.7% | 291.3 / NA | 75 | K435N (AAA→AAC) | mrdA | peptidoglycan DD‑transpeptidase MrdA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (38/36); minor base C (0/1); total (38/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGG > NZ_CP009273/662122‑662612 | cGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGgtt < 1:761804/250‑1 (MQ=255)cGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGgtt < 2:271245/250‑1 (MQ=255) cAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCgg < 2:725532/250‑1 (MQ=255) gCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGt < 2:716544/250‑1 (MQ=255) tCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAg > 1:219371/1‑250 (MQ=255) aaCCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGtt > 2:12490/1‑250 (MQ=255) gaatgaatATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCgg < 2:71848/250‑1 (MQ=255) atgaatATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGaa > 1:238257/1‑250 (MQ=255) aatATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACg < 1:555958/249‑1 (MQ=255) ccGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCg > 2:62984/1‑250 (MQ=255) aCGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCa < 1:377089/250‑1 (MQ=255) cGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTc > 1:168884/1‑249 (MQ=255) cGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCg < 2:26501/250‑1 (MQ=255) cATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATAcc > 1:727214/1‑250 (MQ=255) cccATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGAc < 1:720679/249‑1 (MQ=255) cATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCAt < 2:26643/250‑1 (MQ=255) tGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTAcc > 2:475040/1‑250 (MQ=255) cggcggTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCAtt < 1:498867/249‑1 (MQ=255) ggcggTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCg < 1:473281/250‑1 (MQ=255) cTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGc < 1:655701/250‑1 (MQ=255) tcatcaGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGc > 2:714183/1‑249 (MQ=255) aGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGcgatcg < 1:295999/249‑1 (MQ=255) tGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCa > 2:256222/1‑250 (MQ=255) gAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGCATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATc > 2:640199/1‑250 (MQ=255) gAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATc < 1:730951/250‑1 (MQ=255) gAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATc < 2:385961/250‑1 (MQ=255) ccTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTa < 1:253311/250‑1 (MQ=255) tCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATag > 1:621176/1‑249 (MQ=255) cATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATag > 1:462854/1‑248 (MQ=255) cATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATag > 2:107487/1‑248 (MQ=255) aTTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATagaag < 1:732149/250‑1 (MQ=255) ttAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATagaat > 2:290293/1‑248 (MQ=255) tAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATagaag > 1:720612/1‑248 (MQ=255) aaTCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAgaagaa < 1:20742/249‑1 (MQ=255) aaTCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGaagaag > 2:715381/1‑250 (MQ=255) ttaGGATCAGCAGTGCCTTCCTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGt < 1:579822/248‑1 (MQ=255) tCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATGCCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGt < 1:649509/250‑1 (MQ=255) cAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTa > 1:455259/1‑250 (MQ=255) aGGATCATAATTGCCTTACCCACCTGGATT‑GGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATc < 2:236836/250‑1 (MQ=255) catcaGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCg > 1:621084/1‑248 (MQ=255) caGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAt > 1:512874/1‑250 (MQ=255) aGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAtt > 1:78282/1‑250 (MQ=255) aGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAtt > 1:119811/1‑250 (MQ=255) gTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAttc > 1:642587/1‑250 (MQ=255) cTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGc > 1:78924/1‑249 (MQ=255) cATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAGCTTATAGAAGAAGGGATCCACAGATTCTTCCCGCGAt > 1:754053/1‑250 (MQ=255) cATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGAt > 1:427030/1‑250 (MQ=255) cATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGAt > 2:21327/1‑250 (MQ=255) aTCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGa < 1:484230/248‑1 (MQ=255) gATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTtg < 1:31741/248‑1 (MQ=255) ggggTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACa < 1:666432/249‑1 (MQ=255) gggTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACAtt < 2:709466/250‑1 (MQ=255) cTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACg < 1:14689/250‑1 (MQ=255) tCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGc < 1:269554/248‑1 (MQ=255) cAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGt < 1:12490/250‑1 (MQ=255) cAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGt < 1:646109/250‑1 (MQ=255) cccTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGccc > 1:267649/1‑249 (MQ=255) ccTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCa > 2:187242/1‑250 (MQ=255) cTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCAt > 1:194920/1‑250 (MQ=255) cTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCAt > 1:347499/1‑250 (MQ=255) tACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTc > 1:424336/1‑248 (MQ=255) ccGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACg < 2:101852/250‑1 (MQ=255) cGGAATGGTGTCACCCTGATAGCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCCTCCGCCATGTCGATACCGGTGTAATGACCATAACCGAATTTTCCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGa < 1:476231/250‑1 (MQ=255) tgtCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGttt > 1:618871/1‑250 (MQ=255) aCCCTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTc < 2:300065/249‑1 (MQ=255) cccTGATACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTcc > 2:744302/1‑249 (MQ=255) ccTGATACCACGGTTTTGTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGa < 2:464640/250‑1 (MQ=255) aTACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCt < 1:256222/250‑1 (MQ=255) tACCACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTg < 2:187715/250‑1 (MQ=255) cACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCCCGATCACGTCTTTCCGAACCTgc > 2:420138/1‑247 (MQ=255) cACGGTTTTTTAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTgg > 1:270677/1‑248 (MQ=255) tttttAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGc > 1:612996/1‑249 (MQ=255) tAAAGCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGccacc < 1:698846/249‑1 (MQ=255) gCGTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCt < 1:539653/250‑1 (MQ=255) gattCTGCGTCCATTCGCTGGTAAGCATGTTTCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGAGATCGATCCCCATATCGTAGGCCACCTGATCGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCCAACCTGGCAGTTGCCACCAGCCTgg < 2:371375/248‑1 (MQ=255) gTTTCTGGTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTgg > 1:567448/1‑250 (MQ=255) tttCTGGTTCCATTCGCGGGTAGGCATGCTGCCGGAACGTTCTTCCGCCAGGCCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGCAGGCCACCTGATAGAAGATGGCATCCGCAGATCCTTCCAGCGATCTTGTGACATTCCGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGCACCTGGCAGTTGCCACCAGCCTggg > 2:558498/1‑250 (MQ=255) | CGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGG > NZ_CP009273/662122‑662612 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |