Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,986,131	C→A	W56C (TGG→TGT)	pgsA ←	CDP‑diacylglycerol‑‑glycerol‑3‑phosphate 3‑phosphatidyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,986,131	0	C	A	100.0%	215.7 / NA	63	W56C (TGG→TGT)	pgsA	CDP‑diacylglycerol‑‑glycerol‑3‑phosphate 3‑phosphatidyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/35);  total (28/35)

ACGCCACCATCTGGGCAGTGGTTTTCACTTTCCCAATCCAGGAGACGGCCACGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACC  >  NZ_CP009273/1985887‑1986371                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 aCGCCACCATCTGGGCAGTGGTTTTCACTTTCCCAATCCAGGAGACGGCCACGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAgcg                                                                                                                                                                                                                                             <  2:231111/250‑1 (MQ=255)   gCCACCATCTGGGCAGTGGTTTTCACTTTCCCAATCCAGGAGACGGCCACGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAgcggc                                                                                                                                                                                                                                           >  1:235389/1‑250 (MQ=255)    ccaccaTCTGGGCAGTGGTTTTCACTTTCCCAATCCAGGAGACGGCCACGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAgcggcg                                                                                                                                                                                                                                          >  2:699896/1‑250 (MQ=255)        caTCTGGGCAGTGGTTTTCACTTTCCCAATCCAGGAGACGGCCACGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGc                                                                                                                                                                                                                                       >  1:618621/1‑249 (MQ=255)              gtctggggTTTTCCCTTTCCCAATCCAGGGGCCGGCCACGCTGCTGCGTTTCCCCAGGCCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTGGCCGCCGGTAATGTCACCCACCAGCTGTTATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCGCCAAACCGGGTGCTCTGGTTACAGCGGCGTGCCAGaa                                                                                                                                                                                                                                  <  2:344394/242‑1 (MQ=255)                 aGTGTTTTTCACTTTCCCAATCCAGGAGACGGCCACGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCa                                                                                                                                                                                                                             <  2:414709/250‑1 (MQ=255)                       tttCACTTTCCCAATCCAGGAGACGGCCACGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGTCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAAc                                                                                                                                                                                                                       >  1:397736/1‑250 (MQ=255)                                cccAATCCAGGAGACGGCCACGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACcagtcagt                                                                                                                                                                                                               >  2:249649/1‑249 (MQ=255)                                cccAATCCAGGAGACGGCCACGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACcagtcagt                                                                                                                                                                                                               <  2:612909/249‑1 (MQ=255)                                     tCCAGGAGACGGCCACGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCAccgc                                                                                                                                                                                                         <  2:84706/250‑1 (MQ=255)                                       cAGGAGCCGGCCCCGCTACTGCGTTTACCCAACCCCCCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCAccgccg                                                                                                                                                                                                       <  2:198730/250‑1 (MQ=255)                                           agaCGGCCACGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGAc                                                                                                                                                                                                   >  2:154558/1‑250 (MQ=255)                                                    cGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGCCGCAGAAAAt                                                                                                                                                                                          >  2:516582/1‑250 (MQ=255)                                                       tACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAg                                                                                                                                                                                       >  2:75872/1‑250 (MQ=255)                                                                tACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGcggcgg                                                                                                                                                                               >  2:216634/1‑249 (MQ=255)                                                                   ccAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGCCGCAGAAAATGAGCGCGGCGGCaaa                                                                                                                                                                           >  2:684125/1‑250 (MQ=255)                                                                       ccccGCCATCCATGCGCGTAGCGCAGAAATAATAATTACAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGc                                                                                                                                                                       <  2:308403/248‑1 (MQ=255)                                                                        tCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGc                                                                                                                                                                       >  1:613203/1‑249 (MQ=255)                                                                                  atTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCaaa                                                                                                                                                                           >  1:565641/1‑235 (MQ=255)                                                                                      gcgTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGc                                                                                                                                                        <  1:683992/250‑1 (MQ=255)                                                                                       cgTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAgg                                                                                                                                                         >  2:783310/1‑248 (MQ=255)                                                                                        ctgggccaaaaaTAAAAATTCCAG‑GGGCGACCACCGTTGCCGCCGGTAATGCCACCCACCAGCTGTGAAAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAg                                                                                                                                                      <  2:6136/241‑1 (MQ=255)                                                                                         tAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAgg                                                                                                                                                         >  1:684568/1‑246 (MQ=255)                                                                                         tAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGa                                                                                                                                                     <  1:120179/250‑1 (MQ=255)                                                                                         tAGCGCAGAAA‑AAAAAATTCACGGGGCAACCATCGTGCCCGCGGGTAATGTCCCCCCCCGGCGGGAATAATGCTCGGTTACCGGCCCCATGGCGATACCCCGGAAACCTTAATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACCCTGGTTACAGCGGCGGGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGa                                                                                                                                                     <  2:451292/250‑1 (MQ=255)                                                                                           ccgaaaaaaaaaTAATTCCAC‑GGGCGATCATCGTTCCCGCCGGAAATGTCCCCCCCCAGCGGGGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATa                                                                                                                                                   <  2:337610/244‑1 (MQ=255)                                                                                            cgcAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATa                                                                                                                                                   >  1:502494/1‑249 (MQ=255)                                                                                            cgcAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGCGACAGGCAGATa                                                                                                                                                   >  2:768966/1‑249 (MQ=255)                                                                                                                         atcGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGa                                                                                                                      >  2:46387/1‑250 (MQ=255)                                                                                                                               gccgccGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGacac                                                                                                                >  1:777250/1‑250 (MQ=255)                                                                                                                               gccgccGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCAGGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGaca                                                                                                                 <  2:8350/249‑1 (MQ=255)                                                                                                                                  gccgGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACgg                                                                                                              >  1:210907/1‑249 (MQ=255)                                                                                                                                    cgGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGaa                                                                                                            >  1:428766/1‑249 (MQ=255)                                                                                                                                          tGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTa                                                                                                     <  1:555202/250‑1 (MQ=255)                                                                                                                                             cacccaccAGCTGTGATAATGCTCGTTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTAAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGc                                                                                                  <  1:667929/250‑1 (MQ=255)                                                                                                                                              acccaccAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCa                                                                                                 <  1:20977/250‑1 (MQ=255)                                                                                                                                                   ccaGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTa                                                                                            <  1:697545/250‑1 (MQ=255)                                                                                                                                                    caGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAg                                                                                           <  1:353865/250‑1 (MQ=255)                                                                                                                                                     aGCTGGGATAAGGCTCGGTTACCAGACCCAGGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAgg                                                                                          <  2:468336/250‑1 (MQ=255)                                                                                                                                                          tgATAATGCGCGGTTACCAGCCCCAGGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGatat                                                                                     <  2:35730/250‑1 (MQ=255)                                                                                                                                                               aTGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTaaa                                                                                 <  1:584689/249‑1 (MQ=255)                                                                                                                                                                      gtacccAGCCCCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCCCCAAACCGGGTACTCTGGTTACAGCGGCGTCCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATaa                                                                         <  2:681118/246‑1 (MQ=255)                                                                                                                                                                           aaGCACCATGGCGATAGCCACGAGAACTTTACCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCGGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACg                                                                    <  2:746583/249‑1 (MQ=255)                                                                                                                                                                                      cGATAGCCACGAGAATTTTAGCGGCCACAGGGTAAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAAAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCt                                                         <  2:82218/250‑1 (MQ=255)                                                                                                                                                                                              aagagaACTTAACCTGCCACAGGATCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAg                                                 <  2:370690/248‑1 (MQ=255)                                                                                                                                                                                                gagaACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTa                                               <  1:251773/250‑1 (MQ=255)                                                                                                                                                                                                       ttATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTAc                                        >  1:276940/1‑250 (MQ=255)                                                                                                                                                                                                        tATCGGCCAAAAGGACAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGGGCCAGAAAACCATCGAACCAGACAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTa                                         <  1:481809/248‑1 (MQ=255)                                                                                                                                                                                                           cTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATACTGACGGGTAACTATATGTTGTCAGTAAGATTAcccc                                     >  2:714209/1‑249 (MQ=255)                                                                                                                                                                                                           cTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTAcccc                                     >  1:582963/1‑249 (MQ=255)                                                                                                                                                                                                                 caGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTg                              <  1:116506/250‑1 (MQ=255)                                                                                                                                                                                                                 caGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTg                              <  1:759269/250‑1 (MQ=255)                                                                                                                                                                                                                  aGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGc                             <  1:455346/250‑1 (MQ=255)                                                                                                                                                                                                                       cAAGGAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACag                        >  2:464435/1‑250 (MQ=255)                                                                                                                                                                                                                          ggAAAGCACCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAgaga                     >  2:716154/1‑250 (MQ=255)                                                                                                                                                                                                                                 aCCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAAt              <  2:613203/250‑1 (MQ=255)                                                                                                                                                                                                                                 aCCAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAAt              >  2:236535/1‑250 (MQ=255)                                                                                                                                                                                                                                  ccAAACCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATg             >  2:262434/1‑250 (MQ=255)                                                                                                                                                                                                                                   cAAACCGGGTACTCTGGTCACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCGTAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGt            <  2:614190/250‑1 (MQ=255)                                                                                                                                                                                                                                    aaaCCGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGtt           >  2:468838/1‑250 (MQ=255)                                                                                                                                                                                                                                        cGGGTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCaa       <  1:249649/250‑1 (MQ=255)                                                                                                                                                                                                                                          ggTACTCTGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACg     <  1:629257/250‑1 (MQ=255)                                                                                                                                                                                                                                              ctctGGTTACAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGAcc  <  2:618621/249‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 ACGCCACCATCTGGGCAGTGGTTTTCACTTTCCCAATCCAGGAGACGGCCACGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAC‑GGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACC  >  NZ_CP009273/1985887‑1986371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑