Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,133,642	Δ1 bp	coding (1452/1854 nt)	asmA ←	outer membrane assembly protein AsmA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,133,642	0	A	.	93.8%	272.1 / 5.3	64	coding (1452/1854 nt)	asmA	outer membrane assembly protein AsmA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (46/14);  minor base C (0/4);  total (46/18)
Fisher's exact test for biased strand distribution p-value = 4.82e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGT  >  NZ_CP009273/2133397‑2133882                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 tAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGc                                                                                                                                                                                                                                             <  1:734774/250‑1 (MQ=255)    aCCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCtt                                                                                                                                                                                                                                          <  2:336358/250‑1 (MQ=255)      ccGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTca                                                                                                                                                                                                                                        <  1:891054/250‑1 (MQ=255)      ccGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTca                                                                                                                                                                                                                                        >  1:19061/1‑250 (MQ=255)      ccGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTca                                                                                                                                                                                                                                        <  2:916630/250‑1 (MQ=255)        gCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTcaca                                                                                                                                                                                                                                      <  1:480242/250‑1 (MQ=255)                  tGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCAt                                                                                                                                                                                                                            >  1:528236/1‑250 (MQ=255)                     cGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTAc                                                                                                                                                                                                                         >  1:421418/1‑250 (MQ=255)                          ctttcAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGctc                                                                                                                                                                                                                     >  2:53986/1‑249 (MQ=255)                             tcAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTAcc                                                                                                                                                                                                                 >  1:528184/1‑250 (MQ=255)                                    atcaatcaGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCt                                                                                                                                                                                                          >  1:37329/1‑250 (MQ=255)                                       aatcaGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGaa                                                                                                                                                                                                       >  2:548328/1‑250 (MQ=255)                                        atcaGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAat                                                                                                                                                                                                      >  2:791769/1‑250 (MQ=255)                                                 cTTTCCCCTTCCCAGCCCCCCCCGCCCCGAATATCAACCGGGGTGTCGAAGTTTTGATCTGCCAGATTCAACATCCCTCCCCCTGTCAGCGCCAGCACAGGCGT‑TTGCCCTTGCATGTCTTTAAACGTCCCGCCGCCATCCTTCAACGTAAAATCGGTGTTAAAGCGGTCAAGCCGCGTCACTTTATAAAATTTTTC‑GCGCCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATctgctg                                                                                                                                                                                             <  1:207579/250‑1 (MQ=255)                                                  tttCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATctgctg                                                                                                                                                                                             >  1:364244/1‑249 (MQ=255)                                                     ccccGTCCCAGCCACCCACGACCCGAATAACAAGCTGGGTGTCGCAGTTTGGATCTGCCAGTTTCAACATGCTTCCCCCGGCAAGCCCAAGCAAGGGATA‑TTGACTTTGAATGTCTTTAACCGTCACGCCCCCCTCCTACAACGTAAAATGGGTGGAAAAGCGGTAAAGCCGCGTCACTTTATCAATGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAg                                                                                                                                                                                         <  2:66532/250‑1 (MQ=255)                                                            ccAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATcc                                                                                                                                                                                  >  1:383575/1‑250 (MQ=255)                                                                    ccacGACCCGAATATCAAACGGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAATTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATg                                                                                                                                                                          <  1:41167/250‑1 (MQ=255)                                                                     cacGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATgc                                                                                                                                                                         >  2:580958/1‑250 (MQ=255)                                                                                 acaaaccTGGGTGTCCCGGTTTTGCTCGGCCAGATTCAACTTCCCTTCCCCTGCCCGCCCCAGCACAGGCGA‑TTGCCCTTGCATTTCTTTTAACGTCACGCCCCCACCCTTCAACTTAAAAGCGGTGGAAAGGCGGCCACGCCGCGTCACTTTATCAAAGTTTTC‑GCGGCCTCCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCa                                                                                                                                                             <  2:40262/244‑1 (MQ=255)                                                                                    aaaCTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCAttt                                                                                                                                                          >  2:513302/1‑250 (MQ=255)                                                                                    aaaCTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCAttt                                                                                                                                                          >  2:933721/1‑250 (MQ=255)                                                                                                   ggTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTc                                                                                                                                           >  2:896953/1‑250 (MQ=255)                                                                                                       tGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTg                                                                                                                                       >  1:190879/1‑250 (MQ=255)                                                                                                                aGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTtgtg                                                                                                                              >  2:711727/1‑250 (MQ=255)                                                                                                                   ttCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCg                                                                                                                           >  1:739639/1‑250 (MQ=255)                                                                                                                   ttCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCg                                                                                                                           >  2:446304/1‑250 (MQ=255)                                                                                                                   ttCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCg                                                                                                                           >  1:62662/1‑250 (MQ=255)                                                                                                                     cAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCCGTTGTGGCgg                                                                                                                          >  2:819003/1‑249 (MQ=255)                                                                                                                     cAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCgg                                                                                                                          >  1:452707/1‑249 (MQ=255)                                                                                                                     cAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCgg                                                                                                                          >  1:153977/1‑249 (MQ=255)                                                                                                                     aaaCACGCTTCCCCCTGCCGGCCCCAGCGCAGGCGA‑TTGCCCTTACATGTCGTTAAACGCCCCGACCCCATCCTTCAACGACAAATGGGTGGTAAAGCGTCCAGGCCGCGCCACTTTATAAAAG‑TTTCCGCGGCCTTCAAATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCgg                                                                                                                          <  2:877952/248‑1 (MQ=255)                                                                                                                      aaCATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGaa                                                                                                                        >  2:732776/1‑250 (MQ=255)                                                                                                                        cATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATg                                                                                                                      >  1:627675/1‑250 (MQ=255)                                                                                                                            ccTCCCCCTGCCAGCGCCA‑CCCAGGGGATTTGACCTGGCATGTCTTTTACCGACACGACGCCATCCTCCAACGTAAATTCGGGGGAAAGGCGTTCAAGCCGCGTCCCTTTATCAAATTTTTC‑GCGCCCTTCACATCCCCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTc                                                                                                                  <  2:409491/250‑1 (MQ=255)                                                                                                                                           cccAGCACAGGCTA‑TTGCCCTTGCATGTCGTTTACCGTCCCGCCGCCACCCTCCACCGACAAAGCGGTGGAAAGGCGGTCACGCCGCGTCCCTTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGc                                                                                                   <  1:18101/249‑1 (MQ=255)                                                                                                                                            ccAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCa                                                                                                  >  1:941951/1‑250 (MQ=255)                                                                                                                                                 acaGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGGGTCGGCATCCATGTCAGCACCg                                                                                               >  2:242558/1‑248 (MQ=255)                                                                                                                                                   agctgA‑TTGACTTTGCATGTCGTTTAACGCCCCCACCCCATCCGCCAATGTAAACTGGGGGGAAGAGGGGCCACGCCGCGTCACTTTAT‑AAAGTTTTGCGCGCCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGgaga                                                                                           <  2:812878/246‑1 (MQ=255)                                                                                                                                                   aGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTGCGCATCTTTGTCCGCACCgggg                                                                                            >  2:78645/1‑247 (MQ=255)                                                                                                                                                     gCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGaaa                                                                                         >  1:688840/1‑250 (MQ=255)                                                                                                                                                                      gTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACa                                                                         >  2:258713/1‑250 (MQ=255)                                                                                                                                                                           ttAACTTCACGACGCCATCCTTCAACATAAAAGCGGGAAAAAAGCGGCAACGCCGCGTCATTTTATAAAAGTTTTC‑GGGGCCTACACATCGCCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACAtt                                                                       <  1:824138/247‑1 (MQ=255)                                                                                                                                                                           taaccgccACGACCCCCCCCTTCAACGTAAAATCGGTGGTAAAGCGGTAAAGCCGCTTCACTTTATCAAATTTTTC‑GCGGCCTTCACATCCCCGCCATCACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACAttt                                                                      <  2:866521/241‑1 (MQ=255)                                                                                                                                                                            tAACGTCACGCCGCCATCCTTCAACGTCAAATGGGTGGTAAAGCGGTCAAGCCGCGTCACTTTATCAAAG‑TTTCCGCGGCTTTCCAATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACAttttt                                                                    <  1:848829/249‑1 (MQ=255)                                                                                                                                                                                    cgacgCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTTCGGTCAAc                                                           >  1:632712/1‑250 (MQ=255)                                                                                                                                                                                     gacgCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATAACCCGCCAGGGAAATTTTTCCGGttc                                                             >  2:418838/1‑245 (MQ=255)                                                                                                                                                                                          caccctaaaaCGTAAAATCGGGGGAAAAGCGGTCAAGCCGCGCCAC‑TTAACAAAAGTTTCCGCGCCCTCCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATc                                                     <  1:693886/242‑1 (MQ=255)                                                                                                                                                                                           aTCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCCTCTCTGTCCGCACCGGGGAAAACACCAGGCAGTGATATTTTTTCGGTCAAc                                                           >  2:207388/1‑243 (MQ=255)                                                                                                                                                                                             ccTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGa                                                  >  1:546581/1‑250 (MQ=255)                                                                                                                                                                                              cTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGa                                                  >  1:521136/1‑249 (MQ=255)                                                                                                                                                                                              cTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGa                                                  >  1:352313/1‑249 (MQ=255)                                                                                                                                                                                                  aaCGTAAAAGCGGTTGAAAAGCGGACAAGCCGCGTCACGTTATCAATGTTTTC‑GGGGCCTTCACCTCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGt                                              <  1:568128/249‑1 (MQ=255)                                                                                                                                                                                                    cGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTa                                            >  1:885607/1‑249 (MQ=255)                                                                                                                                                                                                      tCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAAc                                         >  1:629076/1‑250 (MQ=255)                                                                                                                                                                                                      tCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAAc                                         >  1:17219/1‑250 (MQ=255)                                                                                                                                                                                                               tggtAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCCGTCAACGAAATCGGATAGTTAAACGCCTTCAg                                 >  2:1920/1‑249 (MQ=255)                                                                                                                                                                                                                        ggTCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTAcc                        >  1:286396/1‑249 (MQ=255)                                                                                                                                                                                                                          tCAAGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAAt                     >  1:764744/1‑250 (MQ=255)                                                                                                                                                                                                                            aaGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAAtct                   >  1:708848/1‑250 (MQ=255)                                                                                                                                                                                                                             aGCCGCGTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCCGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAAtct                   >  2:500272/1‑249 (MQ=255)                                                                                                                                                                                                                                cgcgTCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCCGAATGGTACCAATTTCAAc               >  2:458592/1‑250 (MQ=255)                                                                                                                                                                                                                                    tCACGTTATCAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCCGTCAACGAAATCGGATAGTTAAAAGCCCTCAGAATGGGACCAAACTCAACGttt           >  2:830308/1‑250 (MQ=255)                                                                                                                                                                                                                                             cAAAGTTTTC‑GCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGGCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGGTAAACGCCTTCAGAATGGGACCAATCTCAACGTTTTTCAGCCGt  >  2:200770/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 TAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGA‑TTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGT  >  NZ_CP009273/2133397‑2133882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑