| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,257,298 | 0 | T | G | 42.1% | 123.5 / 17.7 | 77 | T12P (ACC→CCC) | prs | ribose‑phosphate diphosphokinase |
| Reads supporting (aligned to +/- strand): ref base T (9/35); new base G (32/0); total (42/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.30e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCCAAAGTAGGGGATAACAGCGGTGATACGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGCCATTGAGCCA > NZ_CP009273/1257052‑1257544 | aGCCAAAGTAGGGGATAACAGCGGTGATACGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCACCGAGTGAAGTGTACAGGCGGTTGGCAAAACGTTGTGC‑TAGTTCCGGGGGGGc > 1:912122/1‑250 (MQ=255) aTAACAGCGGTGATACGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCaaaaa < 1:118710/250‑1 (MQ=255) aGCGGTGATACGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAAAACGTTTTGC‑TGGATACGGGGGGGGGGTACCCGCAAAAAGCTTc > 2:230619/1‑250 (MQ=255) gCGGTGATACGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGGTGGGAAAAAGTTGGGG‑TAGGTCCGGGGGGGGGGTACCAGGAAAAAGGTTCa > 1:68173/1‑250 (MQ=255) gTGATACGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TTGTTCCGGGGGGGGGGTAACAGCAAAAAGCTTCatat > 1:55685/1‑250 (MQ=255) gATACGATCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATGCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCa < 2:496688/250‑1 (MQ=255) aTACGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCa < 2:632462/249‑1 (MQ=255) aCGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATATGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCACGc > 1:584337/1‑250 (MQ=255) gCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTTGGCATAAGATGTGC‑TAGTTTCGGGGGGGCGGTTACAGCAAAAAACCTCctattcagcacgcga > 2:542146/1‑238 (MQ=255) cGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACgaga < 1:147424/248‑1 (MQ=255) aaGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAAc < 1:868912/250‑1 (MQ=255) acgacgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGGTGTGC‑TAGTTCCCGGGGGGCGTTACCAGCAAAAAACTTCATATCAGGCACGGGAAGAACCt > 1:250219/1‑248 (MQ=255) cgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGGAATAAGGTGTGC‑TAGTTCCCGGGGGGGGGTACCCGCAAAAAACGTCCTATCCGGGACGAGAAGAACCCCAGGCa > 2:708239/1‑250 (MQ=255) gCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAAAACGTTGTGC‑TAGTTCCGGGGGGGGGGTACCAGCAAAAAGATTCATATCAGGGCCCAGAAAAAACTCAAGCAt > 2:837594/1‑250 (MQ=255) cAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGGAATACGTTGTGC‑TAGTTTCGGGGGGGCGGTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAAACTCAGGGATg > 1:737791/1‑250 (MQ=255) aGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTTCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCAAGc > 1:680204/1‑250 (MQ=255) cATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAAAAAGTTGGGC‑TAGTTCCGGGGGGGCGTGAACAGCAAAAAACTTCATATCAGGTACAAGAAAAACATCAggaaatggcc > 2:208134/1‑241 (MQ=255) aaCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑Ttg < 2:168304/250‑1 (MQ=255) aaCGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGGAATAACTTGTGC‑TAGTTCCGGGGGGGGGGTTCCAGCAAAAAGCTTCCTATCAGGCCCGAGGAGAACCCTCGGCATGCGTCCC‑TTGGTGGa > 2:754347/1‑249 (MQ=255) cGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAg < 1:402876/250‑1 (MQ=255) gACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTTC‑TAGTTTCGGGGGGGCGGTACCAGCAAAAAACTTCATATCAGGCACGAGAAGAACCCCAGGCATGCGGCCA‑TTTGTGGAAAg > 1:802816/1‑249 (MQ=255) aaTTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGG‑TAGGTCCGGGGGGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGGCCA‑TTGGTGGAAAGAAttt > 1:209270/1‑248 (MQ=255) aTTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGGGGCGTTACCAGCAAAAAGCTTTCTATCAAGCACGAGAAGAACCTCCGGCATGCGTCCC‑TTGGTGGTAAGTAttct > 2:212363/1‑247 (MQ=255) ccATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCCATACGTTGGGC‑TAGTTCCGGGGGGGGGTTTCCAGCAAAAAACTTCATATCAAGCCCGAGAAGAACCTCCGGCATGCGGCCC‑TTTGTGGGAAGAAACTGCCg > 2:916822/1‑250 (MQ=255) cATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCg < 1:783318/249‑1 (MQ=255) cATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATAAGTTGTGC‑TAGTTCCGGGGGGGCGGTACCAGCAAAAAACTTCATATCAGGCACGAGAAGAACCTCAGGCCTGCGGCCATTTGGTGG‑AAGAATCTGCCg > 2:213384/1‑249 (MQ=255) gTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCgg < 2:887439/249‑1 (MQ=255) gTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGGGGCGTTACCCGCAAAAAGCTTCATATCAGGCACGAGGAGAACCTCCGGCATGCGGCCC‑TTGGTGGAAAGAATCTGCCGCAAAATGTGCggg > 2:552053/1‑250 (MQ=255) gtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTTGCAATACGTTGTGC‑TTGTTCCGGGGGGGGGGTAACAGCAAAAAACTTCCTATCAGGCACGAGGAGAAACTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGGCGAAAACTGTGCggggccg > 2:662482/1‑246 (MQ=255) gtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCa < 1:562528/249‑1 (MQ=255) gCACAAGTTGACTGGATGATGAAAATATCACCACCGCGTACATGTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGa < 2:748333/250‑1 (MQ=255) aaGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGGGGGGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAAATGTGCGGGCCAGGCATGATCCt > 1:831580/1‑250 (MQ=255) gACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCa < 1:253013/250‑1 (MQ=255) aCTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAg < 1:397859/250‑1 (MQ=255) tgatgaAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGgtg < 1:234513/250‑1 (MQ=255) gatgaAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATAAGTTGTGC‑TAGTTCCGGGGTGGGGTTACCAGCAAAAAGCTTTATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGGGGAAAGAAACTGCCGAAAAATGTGCGGGCCAGGCATGATCCTTTCCAGGCgggg > 1:64730/1‑247 (MQ=255) atgaAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑Ta < 1:287448/250‑1 (MQ=255) tCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAagag < 1:169725/249‑1 (MQ=255) caccacCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGGGGCGGTACCCGCAAAAAGCTTCATATCAGGCACGAGAAGAAACTCAGGCATGCGTCCA‑TTGGTGGGAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGAACCTTTCCAGGCGGGG‑TATTTAAGAgcg > 2:119533/1‑250 (MQ=255) ccaccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGGACTACCTTGTGC‑TAGTTCCGGGGGGGCGTTACCCGCAAAAAACTTCATTTCAGGGACGAGAAGAACCTCAGGCATGCGTCCC‑TTGGTGGAAAGAAACTTCCGAAAACAGAGCGGGCCAAGAATGATCCTTTTCAGGCGGTG‑TATTAAAAAGagcg > 2:593468/1‑250 (MQ=255) accGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGAt < 1:522758/250‑1 (MQ=255) cgTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCCATAAGTTGTGG‑TTGTTTCGGGGTGGGGGTACCACCAAAAAGCTTCATATCAGGCACGAGAAAAACCTCCGGCCAGCGTCCC‑TTGGTGGAAAAAATCTTGCGAAAAATGTGCGGGCCCGGCCTGATCCCTTCCCGGGGGTG‑GATTTAAGAGCGCGGTggc > 2:486171/1‑247 (MQ=255) gTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAAc < 1:302744/250‑1 (MQ=255) tACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGGGGCGTTAACAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCC‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCCTGATCCTTTCCAGGCGGGG‑TATTAAAGAGCGCGATGCAACg > 1:516928/1‑250 (MQ=255) aTTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTTGCAAAACGTTGTGC‑TAGTTCCGGGGGGGCGTTACCCGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGGAAAAATCTGCCGCAAACAGTGCGGGCCAGGCATGATCCTTTCCAGGCGGGG‑TATTAAAGAGAGCGATGCCAccgct > 2:125985/1‑246 (MQ=255) ttttCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGCAGTGTACAGGCGGATGGCAATACCATGTGCTTGGTTCCGGGG‑GGCGTTAACAGCAAAAAACTTTCTATCAGGCACGAGAAGAAACCCCGGCATGCGTCCC‑TTGGTGGGAAAAATCCGCCCAAAACTGTGCCGGCCCGGCATGATCCCTTCCAGGCGGCG‑GTTTAAAAAGCGCGGTGcccacgctt > 2:98164/1‑242 (MQ=255) ttttCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑Ttgg > 1:458188/1‑169 (MQ=255) tttCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑Ttggt < 2:458188/169‑1 (MQ=255) aTTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACa < 1:607245/250‑1 (MQ=255) aTTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAAAACGTTGTGC‑TAGTTTCGGGGGGGCGTTACCAGCAAAAAGATTCCTATCAGGCACGAGAAGAAACTCAGGCATGCGTCCC‑TTTGTGGAAAGAAACTTCCGAAAAATGTGCGGGCCAGGCATGATCCTTTTCCGGCGGGGTTGTAAAAAAGCGCG‑TGCCACGTCTGGAAccacgg > 2:871684/1‑245 (MQ=255) tACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGt < 1:820693/250‑1 (MQ=255) gCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGGAATACGTTGGGC‑CCGTTCCGGGGGGGCGTTACCAGCCAAAAGCTTACTATCATGCACGAGAAGAAACTCAGGCCAGCCTCCA‑TTGGGGGGAAGAATCTGCCGAAAACTGCGTGGGCCAGGGATGGACCTTTCCAGGCGGTG‑AATTAAAAAGGGCGATTGAACGCCTGGAACCAAGT‑GACGTTGt > 2:525895/1‑250 (MQ=255) aCTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAGCTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCa < 2:711085/248‑1 (MQ=255) cTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGGGGCGTTACCAGCAAAAAACTTCATATCAGGCACGAGAAGAACCTCCGGCATGCGTCCA‑TTTGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGGG‑TATTAAAAAGCGCGATGCAACGTCTGGAACAAGGG‑GACGTTGTCAcc > 1:591930/1‑249 (MQ=255) aaaGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTTGCAATACGTTGTGC‑TAGTTCCGGGGGGGGGGTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCCTGCGGCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCCGGCATGATCCTTTCCAGGGGGGG‑TATTTAAGAGCGCGATGCCACGGCTTGGACAAGGG‑GGAGGTGGCACCCCAAATCAGCCTGc > 2:237624/1‑250 (MQ=255) aGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGccc < 1:849979/250‑1 (MQ=255) gCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAAAACGTTGTGC‑TAGTTCCGGGGGGGGGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCCGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAAATGTGCGGGCCCGGCATGATCCTTTCCAGGCGGGG‑TTTTTAAAAGGGCGGTGCAACGTCTGGAAAAAGGG‑GACGTTGTCACCGAAACTCAGCTTGCCCg > 2:627541/1‑250 (MQ=255) gACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGGGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAAACTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGG‑GACGTTGTCACCGAAACTCAGCTTGCCCGGc > 1:887020/1‑250 (MQ=255) aCCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGCCCGGCt < 1:34947/250‑1 (MQ=255) ggcgTCGCCGAGTGAAGTGTACAGGCGGTTGGCTATACGTTGGTC‑TTGTTCCGGGGGGGGGGTTTCAACAAAAAGGTTCATATCCAGCACCAGAAGGAACTCAAGGATGTTATCA‑TTCGTGTGAAGAATCCGCCGAAAACTTTGCGGGGCCCGGATGAAGCTTTTCACGCCGCG‑GATTTAACAATGCGGTGTAACCACTGGAAAAAAGTCGCCGTTTTACCCGAAA‑TCACATTGCCCGGCTTtaaagg > 2:744289/1‑245 (MQ=255) gTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGGTCCGGGGGGGCGGTACCAGCAAAAAACTTCATATCCGGCACGAGAAGAAACCCCGGCATGCGGCCA‑TTGGTGGGAAGAAACTGCCGAAAACTGTGCGGGCCAGGCATGGTCCTTTTCCGGCGGGG‑GATTTAAAAGGGCGGTGCAACGTCCGGAACAAAGT‑GACGTTGTTACCGAAACTCAACTTTCCCCGCTTTAAACAAGGct > 2:771584/1‑250 (MQ=255) tCGCCGAGTGAAGTGTACAGGCGGTTGGAAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGctc < 2:886462/250‑1 (MQ=255) gCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCtg < 2:150406/250‑1 (MQ=255) tGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAAt < 2:55685/250‑1 (MQ=255) aGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATggg < 2:385950/250‑1 (MQ=255) gtgtACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATgggg < 1:486171/250‑1 (MQ=255) tACAGGCGGTTTGCAATAAGTTGTGC‑TAGTTTCGGGGGGGCGTTACCAGCAAAAAGCTTCATTTCAGGCACGAGAAGAACCTCAGGCATGCGGCCC‑TTGGTGGAAAGAATCTGCCGCAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGGG‑TATTTAAAAGCGCGATGCCACGCCTGGGACAAAGT‑GACGTTTTCACCGAAACCCAGCTTTGCCGGCTTTAAGCATCGCTCCTGGCCATggggc > 2:211326/1‑247 (MQ=255) tACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGaa < 2:797817/249‑1 (MQ=255) gCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTa < 2:106365/250‑1 (MQ=255) gCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGGACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGGGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCtt < 1:171118/250‑1 (MQ=255) gCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCt < 1:708239/249‑1 (MQ=255) cGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCca < 2:424842/250‑1 (MQ=255) gC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGc > 2:330801/1‑250 (MQ=255) ttCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGCCAtt > 2:52368/1‑249 (MQ=255) tCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGCCAtt > 1:131563/1‑248 (MQ=255) ggggTGGCGTTACCAGCAAAAAGCTACATAACAGGCACGAGAAGAAACTCAGGCATGCGTCCA‑TTGGGGGAAAGAATCTGCCGAAAACTGTGCGGGACAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCGCCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGCCATTGAGca < 2:354628/250‑2 (MQ=255) gggTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGCCATTGAGCCa > 1:632221/1‑250 (MQ=255) | AGCCAAAGTAGGGGATAACAGCGGTGATACGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC‑TAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA‑TTGGTGGAAAGAATCTGCCGAAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTG‑TATTAAAGAGCGCGATGCAACGTCTGGAACAAGGT‑GACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGCCATTGAGCCA > NZ_CP009273/1257052‑1257544 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |