| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,089,571 | 0 | A | G | 43.3% | 101.3 / 24.2 | 68 | I83V (ATT→GTT) | hisF | imidazole glycerol phosphate synthase subunit HisF |
| Reads supporting (aligned to +/- strand): ref base A (2/36); new base G (28/1); total (31/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.04e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTA > NZ_CP009273/2089336‑2089813 | aCGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGCTCTgg > 1:79117/1‑248 (MQ=255) gaagatgccATGTCTCGACCTTCGTCAGAGTCAAGTAATAAAAGGCTGACAGTTTCGCAACCATGAAAACATTGTCGATAGCGTGTCGCTGGCAAAACGGTACGCTGAAGAAGGCGCTGACGAAGTGGTGTTATAGGATATCACCGCTTCCAGCGATGGGCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTAGGGGAAGTGATCGACATTCCGTTGTGTGTGGGGGGTGGGGTTAAGTCTCTGGAAGAt < 2:155874/243‑1 (MQ=255) cATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGAt < 2:612526/250‑1 (MQ=255) tGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGGTAAGTCTCTGGAAGATGCCGC‑Ggaaa > 2:510746/1‑250 (MQ=255) ttCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCtt < 2:852986/250‑1 (MQ=255) gTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTg < 1:597978/249‑1 (MQ=255) tGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGgc < 2:866890/250‑1 (MQ=255) tGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTTTGTCTCCGGAAGATGCCGC‑GAAAAATCTTTTCTTTGgc > 2:604120/1‑250 (MQ=255) tGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGcgcg < 1:21869/250‑1 (MQ=255) tCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑At < 1:216452/250‑1 (MQ=255) tCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCGGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATGAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑At < 2:740671/250‑1 (MQ=255) cAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGGCTTTTGTGGGTGACGC‑GGAAAATCTTTCCTTTGTGGCgggt > 2:616386/1‑247 (MQ=255) cAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGGTCTTGAAGATGGCGC‑GGAAATTTTTTCCTTTTGTGCggcg > 2:576173/1‑247 (MQ=255) gtggtgAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATaaaa < 2:758503/250‑1 (MQ=255) gtgAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAAtt < 2:157034/249‑1 (MQ=255) tgAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCCGCGATGGCCGAGTGGTAGATAAAAGCTGGGCATCTCGCGCGGCGGCAGTGATCGACATTCCGTTTTGTGTGGCGGGAGGGGTTGAGTGTCTTGGAGGTGGTAC‑GGAAATTCTTTCCTTTAGCGCGG‑GGAGAAtttt > 2:374381/1‑249 (MQ=255) aaaGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCa < 2:922685/250‑1 (MQ=255) aaGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAAGATTTTGGAGATGCCGC‑GAAAATTTTTTTCTTTTGCGCGG‑GGAAAAATTTCAt > 2:430555/1‑250 (MQ=255) gTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGTGTTAGTGTCTGGAAGACGCCGC‑GTAAATTCTTTCTTTTTGCGCGG‑GGAAAAATTCCTACAAca > 2:137427/1‑249 (MQ=255) aGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTcc < 2:113592/250‑1 (MQ=255) cGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTgcg < 1:174003/249‑1 (MQ=255) gCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCt < 1:512994/250‑1 (MQ=255) aCCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGc < 2:853687/250‑1 (MQ=255) aCCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGc < 1:430555/250‑1 (MQ=255) aCCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGc < 1:409331/250‑1 (MQ=255) aCCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGc < 2:475736/250‑1 (MQ=255) aTCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGTTTCTGGGAGATGCCGC‑GGAAAATTTTTTTTTTTGCGCCG‑GAAAAAATTCCATCAACCCTTCTGCGCGCGCAGA‑ACCaa > 2:579570/1‑250 (MQ=255) ggCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGTCTCTGGGAGAGGCCGG‑GAAAATTTTTTTCTTTTGGGCGG‑GTAAAAATTTCATTAACTCTTCTGCGCCGGCCGA‑CCCCACcttta > 2:202644/1‑248 (MQ=255) atatCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGCCCC‑GAAAATTCTTTCCTTTTGCCCGG‑GTAAAAATTTCATCAACTCCCCTGCGCTGGCAGA‑CCCCACAATAAATAc > 1:273807/1‑250 (MQ=255) ccGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTgg < 1:432856/250‑1 (MQ=255) gCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTTAGGCTCTGGGAGATGCCGC‑GAAAATTCTTTTCTTTTGCGCGG‑GTTAAATTTCCATCAACTCTCCCTCGCCGGCAGA‑CCCCACATTAATTTCTCGCCTTGcc > 2:112936/1‑250 (MQ=255) aaaaCGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGc < 2:11775/250‑1 (MQ=255) aaaCGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTTAGTCTCTTGGAGATTCCCC‑GGAAATTTTTTTCTTTGGCGCGG‑GTAAAAATTTCATCAACCCTCCTTCGCCGGCAGA‑CCCCACATTAATTACTCGCCCTGCCCATcccc > 2:466123/1‑247 (MQ=255) aaaCGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGGATCTGGAAGAAGGCGC‑GGAAAATCTTTTCTTTGGGGCGG‑GGAAAATTTTCATCAACTCTCCTGCGCTGGGCGA‑CCCCACATTAATTAATAGCCTCGCCGCACGCt > 2:59928/1‑250 (MQ=255) aaaCGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCt < 1:850299/250‑1 (MQ=255) acgctacgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCttt < 2:711699/250‑1 (MQ=255) acgctacgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGATAAATCTCTGGGAAAAGCCGC‑CAAAAATCTTTCCCTTGGGGCGG‑GTAAAAATTTCATCAACACTCCTGCGCCTGCAGA‑CCCAACCATAAATACTCGCCTGGGCGATCGCctt > 2:845969/1‑250 (MQ=255) cgctacgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTg < 2:610776/250‑1 (MQ=255) aagaagGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGATGCCCC‑GAAAATTCTTTCCTTTTGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAgtg > 1:641263/1‑250 (MQ=255) aagGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAATCTCTGGAAGATGCCGC‑GAAAATTTTTTCCTTTTGCGCGG‑GTAAAATTTCCCTCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTAATCCCCTGGCCGATCGCTTTGGCGTGCAGTGTAt > 1:831356/1‑250 (MQ=255) agGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGGAGATGCCGC‑GGAAATTCTTTTCTTTTGCGCGG‑ATAAAAATTTCATCAACTCTCCTGCGCTGGCAGA‑CCCCACATTAATTAATCGCCTGGCCGATCGCTTTGGCGGGCAGGGGAtt > 2:406187/1‑250 (MQ=255) cTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑GTAAAATTTTCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGt > 1:638626/1‑250 (MQ=255) cGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGt < 1:554020/250‑1 (MQ=255) gAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGGAGATGCCGC‑GCAAAATTTTTTCTTTTGCGCGG‑GTAAAATTTCCCTCAAATCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGGTCGCCTTTGCGGGCAGTGGGTTGTGGTTgggt > 2:254167/1‑248 (MQ=255) tgtTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGATGCCGC‑GAAAAATTTTTTCTTTGGCGCGG‑GTAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATAc > 1:249517/1‑250 (MQ=255) ttCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGGAGATGCCGC‑GAAAATTCTTTCCTTTTGGGCGG‑GTAAAAATTTCCTCAACTCTCCTGCGCTGGCCGACCCCCACA‑TAATTACTCCCCTGCCCGATCGCCTTGGCGTGGCGGGTATTTTGGTCGGGATTGATACCt > 2:644442/1‑250 (MQ=255) ttCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATAcc < 1:607547/249‑1 (MQ=255) cGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTac < 2:910025/250‑1 (MQ=255) gATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTTGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTTGCGTGCAGGGTATTGTGGTCGGTATTTATACCTGGTac > 1:354962/1‑249 (MQ=255) gCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTaa < 1:400787/250‑1 (MQ=255) cGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGAAGCCGC‑GGAAATTCTTTTCTTTTGCGCGG‑GTAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCCACATTTATTACTCGCCTGGCCGCTCGCTTTGGCGTGCAGTGTATTGTGGTCGGGATTGATACCTGGTACGACGCCG‑AAACCCGtga > 2:88405/1‑247 (MQ=255) gATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGATGCCGC‑GAAAATTTTTTCCTTTTGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGGGCAGTGTATTGTGGTCGGGATTGATACCTGGTACGACGCCG‑AAACCGGTaaa > 1:670252/1‑249 (MQ=255) gATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGATGCCGC‑GAAAAATCTTTCCTTTTGCGCGG‑GTAAAATTTTCATCAACTCTCCTGCGCTGGCAGA‑CCCCACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGGGTATTGTGGTCGGGATTGGTACCTGGTACGACGCCG‑AAACCGGGaaa > 1:780644/1‑249 (MQ=255) gATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAat < 1:232088/250‑1 (MQ=255) aaaGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTGAATCAATATAc < 1:293033/250‑1 (MQ=255) cTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTGAATCAATATACCGGc < 1:787138/250‑1 (MQ=255) tATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGGTAAGTCCCTGGGAGGTGGCCC‑GCAAATTCTTTTCTTTTGGGCGGAAAAAAATTTCCTCCAAC‑CTCCTGCGCCGGCAGA‑ACCAACATTAAATACTCTCCCTGCCGGTCGCCTTTGCGTGCCGTGGCTTTTGGTCGGGATTTTTACCTGGTAATACGCCGCAAACCGG‑AAATTACCTGTGAATTAAAATAcccggggtg > 2:774250/1‑242 (MQ=255) gcgTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGcc < 1:887070/249‑1 (MQ=255) cgTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGc < 1:71599/250‑1 (MQ=255) cgTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGGAGAAGGCGCGGAAAATTTTTTCTTTTGGGGGGG‑TAAAAATTTCC‑TCAACTCTCCCGCGCCGGGAGA‑ACCCACAATTATTACTCGCCTTGCCGGTCCCTTTTGCGGGGCGTGGTTTGTGGTCGGGGTTGTTAACCGGGACGACGCCG‑GAAACGGGAAAAATCATGTGGATAAATATACCGGCGGAGaaaaacgc > 2:321183/1‑245 (MQ=255) gAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCcgcg < 1:406187/250‑1 (MQ=255) aaGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGGAGATGCCGC‑GAAAATTCTTTTCTTTTGCGCGG‑GTAAAATTTTCATCAACTCTCCTGCGCTGGCAGA‑CCCCACATTAATTACTCGCCTGGCCGATCGCTTTGGCGGGCGGGGTATTTTGGTCGGGATTGATACCTGGTACGACGCCG‑AAACCGGGAAATATCATGTGAATCAATATACCGGCGAAGAAAGCCGCACCCGCGt > 2:824308/1‑250 (MQ=255) aGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAAGCTCTGGGAGATGCCCC‑GGAAATTTTTTTCTTTGGCGCGG‑AGAAAATTTCCATCAACTCTCCTGCGCTGGCCGA‑CCCAACATTAATTAATCGCCTGGCCGATCCCTTTTGCGTGGAGGGGATTTTGGTCGGGATTGATACCCGGGACGACGCCG‑GAACCGGGAAATATTATGTGGATCAATATACCGGGGCAGAAAGGCGCAccccacgc > 2:32768/1‑245 (MQ=255) gACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCaa < 2:182641/249‑1 (MQ=255) tgtgtgGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTc < 1:719783/249‑1 (MQ=255) tgGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACt < 2:896179/249‑1 (MQ=255) gCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTggg < 1:357260/250‑1 (MQ=255) gTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTa > 2:932855/1‑248 (MQ=255) | ACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGA‑CCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTA > NZ_CP009273/2089336‑2089813 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |