| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 681,493 | 0 | C | A | 48.4% | 6.9 / 15.6 | 32 | S211S (TCG→TCT) | gltJ | glutamate/aspartate ABC transporter permease GltJ |
| Reads supporting (aligned to +/- strand): ref base C (10/6); new base A (15/0); total (26/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.77e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAAACGCATCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAACGACTCCCA‑GGCATGGGCTGAGTAATCGAGC‑AATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGC‑GGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTT > NZ_CP009273/681248‑681704 | aaaccGCACCCCCGCCAGCATCGTGCCCCACAAAATACCAACCCCTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGCTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAACGACt < 1:564146/246‑1 (MQ=255) aTCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAAAGAACCCCA‑AGc > 1:510827/1‑250 (MQ=255) ccGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAACAAACCCCACGGCATg > 1:163162/1‑250 (MQ=255) cAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAACGACTCCCA‑GGCATGGGCTg < 1:280287/250‑1 (MQ=255) cATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGGAAAAGACCCCCA‑GGCAAGGGCTGAg > 1:607288/1‑249 (MQ=255) aTCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAAAGACCCCCC‑AGCATGGGCTGAGt > 1:584097/1‑249 (MQ=255) cGTGCCCCACAAAATACCAACCCCTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAACGACTCCCA‑GGCATGGGCTGAGTAAt < 1:777836/250‑1 (MQ=255) ccccACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAACGACTCCCA‑GGCATGGGCTGAGTAATCGAg < 1:751928/250‑1 (MQ=255) ccccACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAAATCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGGAAAAAAATCCCC‑AGCATGGGGTggggaaaccag > 1:164161/1‑240 (MQ=255) ccACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCCGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAACGTTAATCAACACATACGCCAGCGCAATGGC‑GGGAAAAAAATCCCA‑CGCCTTGGGCGAGTAAACgtgg > 1:464130/1‑246 (MQ=255) cacaAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCCTACATTATTTGCCCCCCCTGTTGCCAGGCAGGCGGACTTTACGTTCAAACAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACCCATACGCCCGCGTACAGGCTGGTAAAGAACCCCCA‑GGAATGGGCGGAGAAATagaga > 2:498929/1‑248 (MQ=255) ccAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAACGACTCCCA‑GGCATGGGCTGAGTAATCGAGC‑AATTTACCCGc < 2:338147/250‑1 (MQ=255) cAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGGAAAAGACTCCCC‑GGCAAGGGGTGGGGAAACGAGC‑AAttttaccccc > 1:897925/1‑243 (MQ=255) aaTCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCCTGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAACGACCCCCC‑GAGATGGGCCGAGTAATTGCGC‑AACTTAccccccc > 1:676903/1‑249 (MQ=255) tCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAACGACTCCCA‑AGCATGGGGTGAGTAAAAGAGC‑AATTTACccgcccgc > 1:119077/1‑250 (MQ=255) cgccgTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAACGACCCCCC‑GGCATGGGCTGGGGAAACGAGC‑AATTTACCCCCCCGCgccgccc > 1:892855/1‑249 (MQ=255) cgccgTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAAAGAATCCCC‑GGCATGGGGTGAGGAAACGAGCAAATTTACCC‑CCCGCgcccccc > 1:324264/1‑249 (MQ=255) ccgTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGGAAAAGACTCCCC‑GGCATGGGGCGAGGAAACCGGC‑AATTTTCCCCCCTGCGCCGCCcta > 1:321688/1‑250 (MQ=255) tGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGGAAACGACTCCCA‑CGCATGGGGTGAGTAAACGAGC‑AATTTTCCCCCCCGCGCCCCCCTATcc > 1:922032/1‑250 (MQ=255) ttttCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAAAGACTCCCC‑CGCATGGGGTGAGGAATAGAGC‑AATTTACCCGCCCGCGCCGCCATATCCACCAGACCg > 1:645731/1‑250 (MQ=255) tCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGGAAAAGACCCCCC‑AGCATGGGGTGGGGAAACGAGG‑AAATTTCCCCCCCGCGCCCCCCTATCCCCCCCACCCCTAATCGAGGCg > 1:711513/1‑250 (MQ=255) atatGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAACGACTCCCA‑GGCATGGGCTGAGTAATCGAGC‑AATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGC‑GGAGTTTTTCAc > 1:91197/1‑248 (MQ=255) atGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAACGACTCCCA‑GGCATGGGCTGAGTAATCGAGC‑AATTTACCCGCCTGCGCCGCCCTATCCACCAGACCGATAGTCGAGGCGATGGC‑GGAGTTTTTTACCAgg > 1:855973/1‑250 (MQ=255) gCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAAAGACTCCCA‑GGCATGGGCTGAGTAATCGAGG‑AATTTACCCGCCTGCGCCGCCAAATCCACCAGACCGATAGTCGAGGCGATGGC‑GGAGTTTTTCACCAGGtt > 2:799130/1‑249 (MQ=255) ggaagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAACGACTCCCA‑GGCATGGGCTGAGTAATCGAGC‑AATTTACCCGCCCGCGCCGCCCTAACCCCCAGACCCAAAGTCGAGGCGATGGC‑GGGGGTTTTTCCCCGGTtcatca > 2:624578/1‑250 (MQ=255) aCAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAAAGACTCCCA‑GGCCTGGGCTGAGTAATCCAGG‑AATTTACCCCCCCGCGCCGCCATATCCACCAGACCGAAAGTCGAGGCGATGGC‑GGGGGTTTTTACCCCGTTCATCACctct > 2:406229/1‑248 (MQ=255) aTGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAAAGACCCCCA‑GGCATGGGCTGAGTAATCGAGC‑AATTTACCCGCCCGCGCCGCCCTATCCACCAGACCGAAAGGCGAGGCGATGGC‑GGAGGTTTTCACCAGGTTCATCATCTCTGCggg > 2:712937/1‑249 (MQ=255) gAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAAAGACTCCCC‑AGCATGGGCCGAGTAATCCAGC‑AATTTACCCGCCTGCGTCGCCACCTCCACCAGACCGAAAGTTCAGGCGACGGC‑CGAGTTTTTCACCACGTTCCTCAtctccgccgtcct > 2:870987/1‑241 (MQ=255) cAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAACGAATCCCA‑GGCATGGGCTGAGTAATCGAGC‑AATTTACCCCCCTGCGCCGCCCTATCCACCAGACCGATAGTCGAGGCGGTGGCGGGGGTTTTTCCCCA‑GTTCATCATCCCTGAGGGCATCGGCGGGGCg > 2:21744/1‑250 (MQ=255) aaCTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAAAGACTCCCA‑GGCATGGGGCGGGGAAACGAGC‑AATTTACCCCCCCGCGCCGCCCTATACACCAGACCGAAAGTCGAGGCGGTGGC‑GGAGGTTTTTCCCAGGGTCAACATTCCCGAGGTCCTCGGCgggggga > 2:465058/1‑245 (MQ=255) aCTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAACGACTCCCA‑GGCATGGGCTGAGTAATCGAGC‑AATTTACCCGCCTGCGCCCCCATATCCACCAGACCGATAGTCGAGGCGGTGGC‑GGAGGTTTTCACCAGGGTCCTCATCTCTGAAGGCCTCGGCGGGGCggt > 2:273179/1‑247 (MQ=255) gCGTAAATCAACACATGCGCCAGCAAAAGGGC‑GATAACCCCCCCCAA‑GGAGGGGGCGGAGAAACCGAAA‑AATTCCCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGC‑GGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCAttttttt < 1:233777/250‑1 (MQ=255) | TAAACGCATCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGC‑GGTAAACGACTCCCA‑GGCATGGGCTGAGTAATCGAGC‑AATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGC‑GGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTT > NZ_CP009273/681248‑681704 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |