Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	479,735	G→T	A42E (GCA→GAA)	acrB ←	efflux RND transporter permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	479,735	0	G	T	99.0%	389.1 / ‑3.7	99	A42E (GCA→GAA)	acrB	efflux RND transporter permease AcrB
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (47/51);  total (47/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01

TGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGCGGTAACTTCTTGTGCTTTTACCTGGACACCAGGACGCACTTTC  >  NZ_CP009273/479489‑479981                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       tgctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCcg                                                                                                                                                                                                                                                     >  2:385492/1‑250 (MQ=255)  gctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCcgg                                                                                                                                                                                                                                                    >  1:386653/1‑250 (MQ=255)   ctgAGCTTCTTGCGGCAGCACCGGCAACGCCAGCTTCAGTTTGTGCTTCCCCTGAACCTGCGCGTTATCCGCAGCAGTACCAGACTCATAGGTCAGGGTGTTCTGCACGGTACCCGTTGTGTCACTGTTAGAGGTCATGTACATCAGGTTATCGATACCGTTCTTATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCATCGCCGGGGTAGGAGGCGGAGATCGTTACTTCcgcc                                                                                                                                                                                                                                                   <  2:364497/250‑3 (MQ=255)      aaCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCcggcgg                                                                                                                                                                                                                                                 <  1:70164/249‑1 (MQ=255)       aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTg                                                                                                                                                                                                                                               >  1:342285/1‑250 (MQ=255)        cttcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTg                                                                                                                                                                                                                                               >  1:573087/1‑249 (MQ=255)          tcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCaa                                                                                                                                                                                                                                            >  2:27302/1‑250 (MQ=255)           ctcgCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCaa                                                                                                                                                                                                                                            <  2:316294/246‑1 (MQ=255)                ggCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAg                                                                                                                                                                                                                                      >  1:820407/1‑250 (MQ=255)                ggCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAg                                                                                                                                                                                                                                      >  2:327840/1‑250 (MQ=255)                  cagcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGa                                                                                                                                                                                                                                    <  1:913436/250‑1 (MQ=255)                        cGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTgc                                                                                                                                                                                                                              <  2:288706/250‑1 (MQ=255)                         ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACGGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTgcg                                                                                                                                                                                                                             >  2:296821/1‑250 (MQ=255)                          gcctCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTgcgc                                                                                                                                                                                                                            <  2:779830/247‑1 (MQ=255)                           cATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTgcgc                                                                                                                                                                                                                            >  1:501892/1‑249 (MQ=255)                               gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCg                                                                                                                                                                                                                       <  2:354627/250‑1 (MQ=255)                               gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCg                                                                                                                                                                                                                       >  2:339998/1‑250 (MQ=255)                               gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCg                                                                                                                                                                                                                       <  2:362549/250‑1 (MQ=255)                                   gCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAg                                                                                                                                                                                                                   <  1:135735/250‑1 (MQ=255)                                   gCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAg                                                                                                                                                                                                                   <  1:830929/250‑1 (MQ=255)                                         gTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGt                                                                                                                                                                                                                  >  2:544178/1‑245 (MQ=255)                                          tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGtt                                                                                                                                                                                                                 <  1:544178/245‑1 (MQ=255)                                                 tGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGc                                                                                                                                                                                                       >  2:688650/1‑248 (MQ=255)                                                   cACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGc                                                                                                                                                                                                   >  2:196371/1‑250 (MQ=255)                                                       tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGccccc                                                                                                                                                                                               >  1:910082/1‑250 (MQ=255)                                                         aaCCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCt                                                                                                                                                                                             <  1:538615/250‑1 (MQ=255)                                                               cgcgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCaa                                                                                                                                                                                        >  1:176343/1‑249 (MQ=255)                                                                  gATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACat                                                                                                                                                                                     >  2:161399/1‑249 (MQ=255)                                                                       ccGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACAtgatga                                                                                                                                                                                >  1:617647/1‑249 (MQ=255)                                                                           aTCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATg                                                                                                                                                                           <  1:429667/250‑1 (MQ=255)                                                                           aTCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATg                                                                                                                                                                           <  2:29835/250‑1 (MQ=255)                                                                            tCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATgg                                                                                                                                                                          >  1:61175/1‑250 (MQ=255)                                                                                   cAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCAc                                                                                                                                                                   >  2:650909/1‑250 (MQ=255)                                                                                      aCTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCa                                                                                                                                                                >  1:237937/1‑250 (MQ=255)                                                                                       cTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCAc                                                                                                                                                               >  2:50024/1‑250 (MQ=255)                                                                                          aaaGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGc                                                                                                                                                             >  2:497895/1‑249 (MQ=255)                                                                                          aaaGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCa                                                                                                                                                            <  2:720360/250‑1 (MQ=255)                                                                                                 aGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATc                                                                                                                                                      <  2:391875/249‑1 (MQ=255)                                                                                                 aGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCg                                                                                                                                                     >  1:362081/1‑250 (MQ=255)                                                                                                     tGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcg                                                                                                                                                 <  2:841847/250‑1 (MQ=255)                                                                                                      gATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcga                                                                                                                                                >  2:488510/1‑250 (MQ=255)                                                                                                          tGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcgatcga                                                                                                                                            >  1:774612/1‑250 (MQ=255)                                                                                                            cACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcgatcgat                                                                                                                                           >  1:65226/1‑249 (MQ=255)                                                                                                            cACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcgatcgat                                                                                                                                           <  1:352282/249‑1 (MQ=255)                                                                                                            cACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATa                                                                                                                                          <  1:433968/250‑1 (MQ=255)                                                                                                             aCGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATa                                                                                                                                          >  2:797186/1‑249 (MQ=255)                                                                                                                gTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAg                                                                                                                                       >  2:616933/1‑249 (MQ=255)                                                                                                                   cccGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGaaa                                                                                                                                    <  1:691638/249‑1 (MQ=255)                                                                                                                    ccGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAAtt                                                                                                                                  <  2:784967/250‑1 (MQ=255)                                                                                                                      gTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAg                                                                                                                                <  2:940752/250‑1 (MQ=255)                                                                                                                      gTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAg                                                                                                                                <  1:327840/250‑1 (MQ=255)                                                                                                                       tGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAgg                                                                                                                               >  2:92646/1‑250 (MQ=255)                                                                                                                              aCTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCt                                                                                                                        >  1:116562/1‑250 (MQ=255)                                                                                                                              aCTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCt                                                                                                                        <  1:672032/250‑1 (MQ=255)                                                                                                                                tGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCtt                                                                                                                       >  1:82282/1‑249 (MQ=255)                                                                                                                                  ttAGAGGACATGTACATCAGGTTATCGATGCCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTa                                                                                                                      <  1:921341/248‑1 (MQ=255)                                                                                                                                            tGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGtt                                                                                                          >  1:520025/1‑250 (MQ=255)                                                                                                                                              tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGttt                                                                                                         <  2:638426/249‑1 (MQ=255)                                                                                                                                                cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCaaa                                                                                                                                                          >  1:937174/1‑198 (MQ=255)                                                                                                                                                 aTCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCaaaa                                                                                                                                                         <  2:937174/198‑1 (MQ=255)                                                                                                                                                        tATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGCCAGTTTGAGAATCGCCAGCCCCCCTCCCAACATGATGATAATGGCGATCCCCCACGCCAAAATCGTGCGAACCATAAAGAAAATTGTCATGTATTAACGGCTCCTGTttaagctaagtcc                                                                                              >  2:319394/1‑247 (MQ=38)                                                                                                                                                          tCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTg                                                                                            >  1:579893/1‑250 (MQ=255)                                                                                                                                                            gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTgg                                                                                           <  2:906427/249‑1 (MQ=255)                                                                                                                                                            gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTgg                                                                                           <  2:472869/249‑1 (MQ=255)                                                                                                                                                            gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGa                                                                                          <  1:806460/250‑1 (MQ=255)                                                                                                                                                              tACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACt                                                                                        >  1:855477/1‑250 (MQ=255)                                                                                                                                                                   ttCATATCCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGtt                                                                                     >  2:515322/1‑248 (MQ=255)                                                                                                                                                                      atatTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAgg                                                                                 <  2:922991/249‑1 (MQ=255)                                                                                                                                                                         ttCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGa                                                                             <  2:651530/250‑1 (MQ=255)                                                                                                                                                                         ttCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGa                                                                             <  1:282358/250‑1 (MQ=255)                                                                                                                                                                            ggtgcGATAACCTGTGTCACCGTGTCCTGCACTGGTGTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGc                                                                           <  2:173267/245‑1 (MQ=255)                                                                                                                                                                                  aTAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCtt                                                                    <  1:796463/250‑1 (MQ=255)                                                                                                                                                                                   tagccTGTGTCACCGTGTGCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTGTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTg                                                                   <  2:266120/247‑1 (MQ=255)                                                                                                                                                                                    aaCCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGc                                                                  >  1:204264/1‑250 (MQ=255)                                                                                                                                                                                    aaCCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGc                                                                  >  2:661044/1‑250 (MQ=255)                                                                                                                                                                                     aCCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCg                                                                 <  2:471735/250‑1 (MQ=255)                                                                                                                                                                                          tgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCtt                                                             <  1:216353/249‑1 (MQ=255)                                                                                                                                                                                             cACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTg                                                         >  1:12339/1‑250 (MQ=255)                                                                                                                                                                                               ccGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGt                                                       <  2:352884/250‑1 (MQ=255)                                                                                                                                                                                                cGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGtt                                                      <  1:48616/250‑1 (MQ=255)                                                                                                                                                                                                 gtgtCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGtta                                                     <  2:910082/250‑1 (MQ=255)                                                                                                                                                                                                  tgtCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGttat                                                    >  2:395860/1‑250 (MQ=255)                                                                                                                                                                                                         cACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGc                                             >  2:71377/1‑250 (MQ=255)                                                                                                                                                                                                          aCTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGCg                                            <  2:193528/250‑1 (MQ=255)                                                                                                                                                                                                           cTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGCgg                                           <  2:500754/250‑1 (MQ=255)                                                                                                                                                                                                                ttCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGCGGTAAct                                      >  2:478377/1‑250 (MQ=255)                                                                                                                                                                                                                      tCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGCGGTAACTTCTtgt                                <  1:385492/250‑1 (MQ=255)                                                                                                                                                                                                                      tCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGCGGTAACTTCTtgt                                <  2:60447/250‑1 (MQ=255)                                                                                                                                                                                                                        aGCGCCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGCGGTAACTTCTTGTGc                              <  1:50024/250‑1 (MQ=255)                                                                                                                                                                                                                         gcgcCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGATTGAGGATCGCCAGCCCACCTGCCAACATGATGATAATGGCGATAACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTCAGTTAAGAATTGGACTGTTCAGGCTGAGAACCGCTTGCGGCTTGTCGGATATTATTAGCGGTAActtactatgat                             >  2:814457/1‑242 (MQ=255)                                                                                                                                                                                                                           gcCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGCGGTAACTTCTTGTGCttt                           <  1:500648/250‑1 (MQ=255)                                                                                                                                                                                                                           gcCGGGGTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGCGGTAACTTCTTGTGCttt                           <  1:783530/250‑1 (MQ=255)                                                                                                                                                                                                                                 gTAGGAGGCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGCGGTAACTTCTTGTGCTTTTACCTg                     <  1:242403/250‑1 (MQ=255)                                                                                                                                                                                                                                    ggaggCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGCGGTAACTTCTTGTGCTTTTACCTGGa                   <  1:797186/249‑1 (MQ=255)                                                                                                                                                                                                                                      aggCGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGCGGTAACTTCTTGTGCTTTTACCTGGacac                >  1:464458/1‑250 (MQ=255)                                                                                                                                                                                                                                         cGGAGATCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGCGGTAACTTCTTGTGCTTTTACCTGGACACCAg             <  1:744655/250‑1 (MQ=255)                                                                                                                                                                                                                                              aTCGTTACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGCGGTAACTTCTTGTGCTTTTACCTGGACACCAGGACGc        <  1:688650/250‑1 (MQ=255)                                                                                                                                                                                                                                                  ttACTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGCGGTAACTTCTTGTGCTTTTACCTGGACACCAGGACGCACtt    >  1:683026/1‑250 (MQ=255)                                                                                                                                                                                                                                                    aCTTCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGCGGTAACTTCTTGTGCTTTTACCTGGACACCAGGACGCCCTTTc  >  2:251489/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       TGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATTATCAGCGGTAACTTCTTGTGCTTTTACCTGGACACCAGGACGCACTTTC  >  NZ_CP009273/479489‑479981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑