Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,009,4750AG40.8% 197.4 / 47.4 128E254G (GAA→GGA) pqiBintermembrane transport protein PqiB
Reads supporting (aligned to +/- strand):  ref base A (11/63);  new base G (51/0);  total (64/64)
Fisher's exact test for biased strand distribution p-value = 1.39e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 130 total aligned reads displayed.

CAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGG  >  NZ_CP009273/1009229‑1009723
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                             
cAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAgg                                                                                                                                                                                                                                                            <  2:125293/250‑1 (MQ=255)
       tCCGGTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTTGGCTTTTTTGTGTCGGGAggggtgggt                                                                                                                                                                                                                                                     >  1:227738/1‑243 (MQ=255)
         cGGTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGGGTCAGGGTTTTTGTGGCGGGAGGGTTGGGttt                                                                                                                                                                                                                                                   >  1:137895/1‑250 (MQ=255)
          ggTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTa                                                                                                                                                                                                                                                  <  1:883547/250‑1 (MQ=255)
           gTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAg                                                                                                                                                                                                                                                 <  2:769936/250‑1 (MQ=255)
             gCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTTATGTGCCGGGAGGGCTGGGTTTAggg                                                                                                                                                                                                                                               >  1:971828/1‑250 (MQ=255)
               tGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGGCTTTTTGTGGCGGGAGGGCTGGGTTTTggg                                                                                                                                                                                                                                               >  2:824311/1‑248 (MQ=255)
                      tGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCCGCGTTGGTTTGCCCGGAGGCGTGGATTTTggggggcggggg                                                                                                                                                                                                                                      >  2:463248/1‑241 (MQ=255)
                      tGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGGGTCAGGTTTTATGTGGCGGGAGGGCTGGGTTTAGGGGAGGCAggg                                                                                                                                                                                                                                      >  1:511765/1‑248 (MQ=255)
                            tCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTTATGTGCCGGGAGGGCTGGGTTTTGGGCAGCCCGTGGCACC‑g                                                                                                                                                                                                                               >  1:419415/1‑250 (MQ=255)
                                gTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCCGGTTTGTTGTGCCGGGAGGGCTGGGTTTTTGGGAGGCAGCGGCAccccac                                                                                                                                                                                                                             >  2:127508/1‑245 (MQ=255)
                                   ggTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTTATGTTGCGGGAGGGCTGGGTTTATGGGCGCCAGTGGGCCC‑Gaaaaaac                                                                                                                                                                                                                        >  1:364979/1‑247 (MQ=255)
                                               aCCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGTCCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTgg                                                                                                                                                                                                            <  2:614738/250‑2 (MQ=255)
                                                 cAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTat                                                                                                                                                                                                          <  1:668669/250‑1 (MQ=255)
                                                 cAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTat                                                                                                                                                                                                          <  1:595561/250‑1 (MQ=255)
                                                  aGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTatg                                                                                                                                                                                                         <  2:323508/250‑1 (MQ=255)
                                                   gCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTGAGCGTTGATGTGTCGGGAGGGGTGGGGTTTGGGGGGGCAGTGGGACC‑GAAAACAGGGTTCGttttttttgg                                                                                                                                                                                                        >  2:652781/1‑244 (MQ=255)
                                                     aCCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGCGTGTGCTTTTATGGGTTGGCAGGTATGGGTTTAGTGGTGCCCGCGGCCCC‑GCAAACAGCTCTTGTTTTTtattatt                                                                                                                                                                                                      >  2:282003/1‑249 (MQ=255)
                                                      ccTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTAtgatga                                                                                                                                                                                                     <  1:433784/250‑1 (MQ=255)
                                                        ttCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTTATGTGCCGGGAGGGCTGGGTTTTGGGCAGCCAGGGGCACC‑GGAAACAACTTTCGTTTTTTATgatgat                                                                                                                                                                                                    >  1:119048/1‑249 (MQ=255)
                                                         tCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGGCCGCTTTTGTGTGCCGGGAGGGCGGGGTTTTGGGGAGGCCGAGGTCCC‑CGAAACAACTTTCCTTTTTTTTgttgat                                                                                                                                                                                                    >  2:199326/1‑248 (MQ=255)
                                                               cacaAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGGCAGCTTTGATGTGGCGGGAGGGCTGGGTTTAGGGGAGCCAGTGGGGCC‑GGAAACAACTTTTGTTTTGTTTTATGATTAGAaga‑g                                                                                                                                                                                           >  1:54667/1‑250 (MQ=255)
                                                                 caAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGGAGGGCTGGGTTTAGGGCAGCCAGGGGGACC‑GAAAACAGCTTTTGTTTTTTATGATGGTCAGAaga‑gaa                                                                                                                                                                                         >  1:547422/1‑248 (MQ=255)
                                                                 caAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGGGTCTGCTTTGTTGTGGCGGGAGGTCTGGGTTTTGGGCAGCCAGGGGCACC‑GGAAACAGCTTTTGTTTTTTATGATGTTCAGAaga‑gaa                                                                                                                                                                                         >  1:23577/1‑248 (MQ=255)
                                                                     aaCGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCa                                                                                                                                                                                     <  1:722235/250‑1 (MQ=255)
                                                                      aCGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTTTTGTGGCGGGAGGGCTGGGTTTTTGGGAGGCCGTGGGACC‑CAAAAAAACCTTTGTTTTTTTTGATGGTTAGAAGA‑GGAttt                                                                                                                                                                                      >  1:767826/1‑247 (MQ=255)
                                                                      aCGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAg                                                                                                                                                                                    <  1:282003/250‑1 (MQ=255)
                                                                        gCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAATAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGa                                                                                                                                                                                  <  2:173715/250‑1 (MQ=255)
                                                                                    aTCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTTATGTGGCGGGAGGGCTGGGTTTTGGGGAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTTTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTtcac                                                                                                                                                                      >  1:332782/1‑250 (MQ=255)
                                                                                      cAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTTAGCTTTGATGTGGCGGGAGGGCTGGGTTTTGGGGAGCCAGGGGCACC‑GGAAAAAACTTTTGGTTTGTTTGGTGATCAGAAGA‑AGAATCAGGAATCGGTGTTCAACg                                                                                                                                                                    >  1:522755/1‑250 (MQ=255)
                                                                                       aaCTGTTCATCCATGCACCTTATGACCGACTGGCGACCAACAATGTTCGCTGCTGGAAAGATAGTGGCGTTGCGGTTGATCGGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGAGGCGGTGTCAGCTTTGATGTGCCGGTAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTACGTTGTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGa                                                                                                                                                                   <  2:605027/250‑1 (MQ=255)
                                                                                       aaCTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGa                                                                                                                                                                   <  1:104666/250‑1 (MQ=255)
                                                                                          tGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCGGCTTTGATGTGGCGGGAGGGCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAAAAGCCTTCGTTTTGTATGATGTACAGAAGA‑GCATTTCGGATTCGGTGTACACCGATca                                                                                                                                                                >  2:39447/1‑250 (MQ=255)
                                                                                             tcatcaATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTTAGCCTTTATTTGCCGGGAGGGCTGGGTTTTGGGGAGCCAGGGGCACC‑GGCAAAAGATTTCGTTTTGTATGATGATCAGAAGA‑GCATTTAGGATTCGTTTTTAACCGATCACAt                                                                                                                                                             >  1:614851/1‑250 (MQ=255)
                                                                                                   aTGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTa                                                                                                                                                       <  2:884083/250‑1 (MQ=255)
                                                                                                        ccTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGa                                                                                                                                                  <  1:134039/250‑1 (MQ=255)
                                                                                                        ccTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGa                                                                                                                                                  <  1:754041/250‑1 (MQ=255)
                                                                                                        ccTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGGGTCAGGTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTACGTTTTGGATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGa                                                                                                                                                  <  2:926913/250‑1 (MQ=255)
                                                                                                             tGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTc                                                                                                                                             <  1:173481/250‑1 (MQ=255)
                                                                                                                     tGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGCGTGCGGTTTGCTGTGCCTGCGGGGCGGGGTTTTCGGCCGCCCGCGGCACC‑GCAAACACCGTTTGTTTTGTTTGTTTAACAGAAAG‑GGATTTACGATTCTCTGTTCACCAAACACCTTGATTATCTGAGGTTTTTTtaaaa                                                                                                                                     >  2:640761/1‑248 (MQ=255)
                                                                                                                       gTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGAtt                                                                                                                                   <  1:357439/250‑1 (MQ=255)
                                                                                                                       gTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGAt                                                                                                                                    <  2:393680/249‑1 (MQ=255)
                                                                                                                           ccAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGt                                                                                                                               <  1:306443/250‑1 (MQ=255)
                                                                                                                           ccAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGt                                                                                                                               <  1:41987/250‑1 (MQ=255)
                                                                                                                           ccAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGt                                                                                                                               <  1:231721/250‑1 (MQ=255)
                                                                                                                              acaaTGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTAcg                                                                                                                            <  1:269090/250‑1 (MQ=255)
                                                                                                                                aaTGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGACGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTAcgcg                                                                                                                          <  2:53418/250‑1 (MQ=255)
                                                                                                                                    ttCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCt                                                                                                                      <  2:134969/250‑1 (MQ=255)
                                                                                                                                       gCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTTATGTGTCGGGAGGGGTTGGGTTTGGGGAGGCAGGGGCACC‑GGAAAAAGCTTTCGTTTTTTATGATGATCAGAAGA‑GGATTCCGGATTCGTTGTTAACCGATCACATTTATTTTTTGTTGTTTTTTTAAGATTTGGTACGCGGTCTGc                                                                                                                    >  2:823345/1‑249 (MQ=255)
                                                                                                                                       gCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTTATGTGCCGGGAGGGCTGGGTTTAGGGGAGCCCGGGGGACC‑GAAAACAGCTTTCGTTTTTTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTTAAGATTCGGTACGCGGTCTGc                                                                                                                    >  1:80025/1‑249 (MQ=255)
                                                                                                                                        cTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGGCTTTATGTGGCGGGGGGGCTGGGTTTTGGGGGGGCCGTGGCGCC‑GCAAAAAGGTTTCGTTTTGTGTGATGATCAGAAGA‑GGAGTCTGGAATCGTTGTTCACCGATTACATTGTTTTTTTGTTGTTCTTTTAAGATTTTGGACACGGGCTGcga                                                                                                                  >  2:203018/1‑248 (MQ=255)
                                                                                                                                           cTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCGTTTGTGTGGCGGGAGGGCTGGGTTTTGGGGAGGCCGTGGCACC‑CAAAAAAGCTTTTGTTTTGTATGATGGTCAGAAAA‑GGATTCAGGGTTCGTTTTTAACCGCTCAAATTGATTATCTTTTGTTTTTTTAAGATTTGGTACGCGGGCTGcgaacg                                                                                                               >  2:733247/1‑245 (MQ=255)
                                                                                                                                              gAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCgg                                                                                                             <  1:493861/248‑1 (MQ=255)
                                                                                                                                              gAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCCTTTATGTGGCGGGAGGGCTGGGTTTTGGGGAGGCCGGGGGGCC‑GGAAAAAGCTTTTGTTTTTTATGATGATCAGAAGA‑GGATTCAGGATTCGTTGTACACCGATCACATTGGTTTTCTGGTGTTTTTTTAAAATTTGGGACGCGGGCTGCAAACCggg                                                                                                            >  1:781385/1‑250 (MQ=255)
                                                                                                                                                aaGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGc                                                                                                         <  1:300503/250‑1 (MQ=255)
                                                                                                                                                 aGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTTATGTGCCGGGAGGTCTGGGTTTAGGGCAGCCAGGGGCACC‑GAAAACAGCTTTTGTTTTGTTTGATGATCAGAAGA‑GCATTCAGGATTTGTTGTACACCGATCACATTGATTATCTGATGTTCTTTTAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCt                                                                                                        >  1:651840/1‑250 (MQ=255)
                                                                                                                                                        ggCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCCGGGTTGTTGTGGCGGGAGGGGCGGGTTTTGGGGGGCCAGGGGGCCC‑GCAAAAAGCTTTCGTTTTTTTTGATGATCAGAAGA‑GAAATCTGGATTCGTTGTGCACCGATCACATTTTTTATTTTATGTTTTTTTAAAATTCTGTACGCGGTGTGC‑AAACCGGAGGTCCgggcg                                                                                                 >  2:758640/1‑247 (MQ=255)
                                                                                                                                                          cATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTagag                                                                                               <  1:415763/250‑1 (MQ=255)
                                                                                                                                                            ttGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTTCGCTTTTATGTGCCGGGAGGTCTGGGTTTTGGGGAGCCCGTGGCAAC‑GGAAACAGCCTTCGTTTTTTTTGATGATCAGGAGA‑GCATTCAGGAGTCGTTGTACACCGATCCCCTTTTTTATCTGATGTTTTTTATAGATTTTGTACTCGGGCTGC‑AACCGGGAGCTCCGGTAGAG‑tt                                                                                            >  2:818741/1‑250 (MQ=255)
                                                                                                                                                               cGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCg                                                                                         <  1:80304/250‑1 (MQ=255)
                                                                                                                                                                ggTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCgg                                                                                        <  2:4332/250‑1 (MQ=255)
                                                                                                                                                                  ttGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCgggg                                                                                      <  2:273801/250‑1 (MQ=255)
                                                                                                                                                                    gATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGt                                                                                     <  2:534487/249‑1 (MQ=255)
                                                                                                                                                                    gATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGGGTCCGCTTTTATTTGGCGGGAGGGGTGGGTTTTGGGCAGCCAGTGGCACC‑CGAAACAGCCTTTGTTTTGTTTGGTGATTAGAAGA‑GCATTCAGGATTCGTTGTTCACCCATCACATTTATTTTCTGATGTTCTTTTAAGATTCTGTATGCGGTCTGC‑AACCCGGGGGTCCGGGGGAGTTTCCggggg                                                                                     >  2:901880/1‑249 (MQ=255)
                                                                                                                                                                      tCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGGAGGGCTGGGTTTAGGGGAGCCCGTGGCACC‑GAAAAAAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTTGTTGTACACCGATCACATTGATTATCTGATGTTCTTTTAAGATTTGGTACGCGGGCTGC‑AACCGGGAGCTCCGGGAGAG‑TTCCGGGGGAtt                                                                                  >  1:261147/1‑250 (MQ=255)
                                                                                                                                                                       cTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGGCAGCGTTTATGTGCCGGGAGGGCTGGGTTTTGGGCAGCCAGTGGCCCC‑CCAAACAACTTTCTTTTTGTATGATGATTAGAAGA‑GGAATCAGGATTTGTTGTTCACCGATCACAATGATTATTTGATGTTTTTTTAAAATTTGGTACGCGGTCTGC‑AACCGGGCGCTCCGGTAGAG‑GTCCggggggttc                                                                                 >  2:607921/1‑245 (MQ=255)
                                                                                                                                                                        tGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGGCAGCTTTTGTGTGGCGGGAGGGCTGGGGTTAGGGGAGCCAGGGGGGCCGGAAAAAAGC‑TTCCTTTTTTTTGATGATTAGAAGAGGAATTCGGGATT‑GTTGTTCACCCAACACATTTATTATCTGGTGGTCTTTTAAAAATTTGTACGCGGTCTGC‑CACCGGGGGCTCCGGTAGAG‑TTCCggggggattg                                                                                >  2:310322/1‑244 (MQ=255)
                                                                                                                                                                          aCGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTAtt                                                                                  <  2:901712/246‑1 (MQ=255)
                                                                                                                                                                            gTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTTATGTGCCGGAAGGGCTGGGTTTTGGGCAGCCAGTGGCACC‑GGAAACAGCTTTTGTTTTTTATGATTATCAGAAGA‑GCATTTAGGGTTCGTTGTACACCGATCACATTGATTATCTGATGTTTTTTTAAGATTCGGTACGCGGGCTGC‑AACCCGGAGCTCCGGTAGAG‑GTCCGGGGGATTTGcccg                                                                            >  1:692356/1‑248 (MQ=255)
                                                                                                                                                                             tCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCAGTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTgg                                                                           <  1:538804/250‑1 (MQ=255)
                                                                                                                                                                               agcagGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTTAGCTTTTATGTTGCGGGAGGTCTGGATTTTGGGGAGCCAGTGGCACC‑CAAAACAGCTTTCGTTTTGTATGGTGATCAGAAGA‑GCATTCAGGATTTCTTGTTCACCGATCACATTTATTATTTGATGTTCTTTAAAGATTTGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTTCGGGGGATTTGCCTggg                                                                          >  1:633267/1‑249 (MQ=255)
                                                                                                                                                                               agcagGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGt                                                                         <  2:969533/250‑1 (MQ=255)
                                                                                                                                                                                gcagGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGGCAGCTTTGATGTGGCGGAAGGTCTGGGTTTAGGGCAGCCAGTGGGACC‑GAAAACAGCCTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTAAACCGATCACATTGATTATTTGATGTTCTTTTAAAATTCGGGACGCGGGCTGC‑AACCGGGGGCTCCGGGAGAG‑GTTCGGGGGATTTGCCTgggga                                                                        >  1:888355/1‑248 (MQ=255)
                                                                                                                                                                                 cagGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTAc                                                                       <  2:46016/250‑1 (MQ=255)
                                                                                                                                                                                     gATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCCGCTTTTATGTGCCGGGAGGGCTGGGTTTTGGGGAGGCAGGGGGACC‑GCAAACAGCCTTTGGTTTTTATGATGATTAGAAGA‑GCAATCAGGATTCGTTGTACACCCATCACATTTATTATCTGGTGTTCTTTAAAAATTTGGTACACGGGCTGC‑AACCCGGGGCTGCCGTAGAG‑GTTCGGGGGGTTTGCCTGGGGACC‑Gt                                                                   >  2:586054/1‑249 (MQ=255)
                                                                                                                                                                                      aTGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTaa                                                                 <  1:945448/250‑1 (MQ=255)
                                                                                                                                                                                           tgtgGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCCGCTTTTATGTGCCGGGAGGTCTGGGTTTAGGGCAGCCAGGGGCACC‑GCAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCCGGATTCGTTGTACACCGCTCACATTGTTTTTTTGATGTTTTTTTAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGGATTCGCCTGGGGACC‑GTAAGCa                                                              >  1:198314/1‑248 (MQ=255)
                                                                                                                                                                                           tgtgGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGGCAGGTTTGGTGTGCCGGGAGGGCTGGGTTTTAGGGAGGCCGGGGGACC‑GGAAAAAGATTTTGGTTTTTTTGATGGTCAAAAAA‑GCAATCAGGGATCGTTGTTAACCGATCACCTTTATTATTTGATGTTTTTTTAAAATTTGGGAAGCGGGCTGC‑CAACGGGGGGTCCGGTAGGG‑TTTCGGGGGATTTGCCTGGGGACC‑GGAAGCaa                                                             >  2:199382/1‑249 (MQ=255)
                                                                                                                                                                                             tgGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAg                                                           <  1:156569/249‑1 (MQ=255)
                                                                                                                                                                                               gagaTGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGTACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGc                                                        <  1:797979/250‑1 (MQ=255)
                                                                                                                                                                                               gagaTGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGc                                                        <  1:72974/250‑1 (MQ=255)
                                                                                                                                                                                               gagaTGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGc                                                        <  1:890716/250‑1 (MQ=255)
                                                                                                                                                                                                    gggCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGGTGTTCCGGGAGGGCCGGGTTTTGGGGAGCCAGGGGCACC‑GGAAAAAACCTTTGTTTTTTTTGATGATCAGAAGA‑GCATTCAGGATTTGTTGTTCACCGATCACCTTTATTATTTGATGTTTTTTTTAAATTCGGTACGCGGTCTGC‑AAACGGGGGCCCCGGGAGAG‑TTTCGGGGGATTCGCCCGGGGACC‑GGAAGCACAAGGCCATTc                                                   >  2:464393/1‑250 (MQ=255)
                                                                                                                                                                                                          aTTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTgc                                              <  1:970639/249‑1 (MQ=255)
                                                                                                                                                                                                          aTTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTGTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTg                                               <  2:759164/248‑1 (MQ=255)
                                                                                                                                                                                                            tGACAACGCTGCTGAGTGGCGGTGTCAGCTTTTATGTGCCGGGAGGGGTGGGTTTTGGGCAGGCCGGGGGACC‑GAAAAAAGCTTTTGTTTTTTTTGATGATTAGAAGA‑GGAATCTGGGATTGTTTTTCACCGGTCACATTGATTTTTTGATGTTCTTTTAAAATTTGGTGAGCGGGGTGC‑AAACCGGGGGTCCCGGAGGG‑TTTCGGGGGAGTTGCCTGGGGACCGGGAAGCAAA‑AGCCCTTCtttttgcc                                           >  2:647937/1‑245 (MQ=255)
                                                                                                                                                                                                               cAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTGCGCCGaa                                        <  2:532821/250‑1 (MQ=255)
                                                                                                                                                                                                                  cGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTGCGCCGAatat                                     <  2:114618/250‑1 (MQ=255)
                                                                                                                                                                                                                          aGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTATGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGa                             <  2:268602/250‑1 (MQ=255)
                                                                                                                                                                                                                              gCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACAtt                         >  1:315035/1‑250 (MQ=255)
                                                                                                                                                                                                                                ggTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACAttt                        >  2:937558/1‑249 (MQ=255)
                                                                                                                                                                                                                                    tCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACga                   <  1:608967/250‑1 (MQ=255)
                                                                                                                                                                                                                                    tCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACg                    <  1:371179/249‑1 (MQ=255)
                                                                                                                                                                                                                                       gCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACgatga                >  2:271557/1‑250 (MQ=255)
                                                                                                                                                                                                                                             aTGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCg          >  1:412143/1‑250 (MQ=255)
                                                                                                                                                                                                                                                tgCCGGAAGGTCTGGATTTAGGGCAGCCTGTTGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTAt       <  1:477713/250‑1 (MQ=255)
                                                                                                                                                                                                                                                  ccGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTAtt      <  1:454410/249‑1 (MQ=255)
                                                                                                                                                                                                                                                  ccGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTAtt      <  1:776003/249‑1 (MQ=255)
                                                                                                                                                                                                                                                      aaGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCgg  >  1:751249/1‑249 (MQ=255)
                                                                                                                                                                                                                                                      aaGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCgg  >  2:421471/1‑249 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                             
CAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACC‑GAAAACAGCTTTCGTTTTGTATGATGATCAGAAGA‑GCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGC‑AACCGGGAGCTCCGGTAGAG‑TTCCGGGGTATTCGCCTGGGTACC‑GTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGG  >  NZ_CP009273/1009229‑1009723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑