| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,089,571 | 0 | A | G | 39.1% | 101.5 / 22.6 | 70 | I83V (ATT→GTT) | hisF | imidazole glycerol phosphate synthase subunit HisF |
| Reads supporting (aligned to +/- strand): ref base A (9/33); new base G (27/0); total (37/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.52e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAG > NZ_CP009273/2089328‑2089820 | cTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGt < 2:790773/250‑1 (MQ=255) cGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAg < 1:568966/250‑1 (MQ=255) cATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGGCTCTG‑GGAGATggcccgg > 1:890708/1‑243 (MQ=255) tctcGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAAt < 1:38189/249‑1 (MQ=255) tctcGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTG‑GGAGATGCCGC‑GAAAAtt > 1:968269/1‑250 (MQ=255) gACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTG‑GTAGATGGCGC‑GCAAATTC‑ttt > 2:312712/1‑250 (MQ=255) ttCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAATCTCTG‑GAGAATGGCGC‑CGCAAttc‑ttttctt > 2:890673/1‑250 (MQ=255) tggtgAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAAt < 2:230377/250‑1 (MQ=255) tgAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTc < 1:340535/250‑1 (MQ=255) aaaGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCa < 2:945453/250‑1 (MQ=255) ggCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCa < 1:719453/250‑1 (MQ=255) gTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAAct < 1:338878/250‑1 (MQ=255) gTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAAct < 2:934300/250‑1 (MQ=255) gCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTG‑GAAGATGCCGC‑GAAAACTCTTTTCTTTTTGCCCGG‑TAAAATTTTCCTCCACTCCCCTCCGCt > 2:90602/1‑250 (MQ=255) cAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTg < 1:130761/250‑1 (MQ=255) aaCCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGATAAGTATCTG‑GGAGAAGGCGC‑GCAAAATC‑TTTTCTTTTGCGGGGGTAAAAATTTCCTAAACCCTCCTCCGCTgg > 2:241243/1‑250 (MQ=255) cATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTTGCGCGGATAAAATTTTCATCAACTCTCCTGCGCTgg > 1:977040/1‑247 (MQ=255) aTTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACat < 1:833991/250‑1 (MQ=255) aTTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTG‑GGAGATGCCGC‑GGAAATTT‑TTTCCTTTGTCGCGGATAAAATTTTCCTCAACTCTCCTGCGCTGG‑CAGACCCAACat > 1:193953/1‑250 (MQ=255) aTTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAATTTTTG‑GAGGATGACGC‑GCAAATTC‑TTTTCTTTGGCGCGGATAAAAATTCCCTCAACTCTCCTGCGCTGG‑CAGACCCCACat > 2:721180/1‑250 (MQ=255) tatCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACt < 2:721264/250‑1 (MQ=255) atCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTTCTTTGGCGCGGATAAAATTTTCATCAACTCTCCTTCGCCGG‑CAGACCCCACAATAAATACTc > 1:59760/1‑250 (MQ=255) gCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGTTCTG‑GGAAATGCCCC‑GGAAATTT‑TTTTCTTTTGCGCGGATAAAATTTCCATCCACTCTCCTTCCCTGGCCGGCCCCAACA‑TAATTAATcccccg > 2:82581/1‑248 (MQ=255) ccgccgGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTG‑GAAGATGCCGC‑GAAAAATC‑TTTTCTTTTGCGCGGATAAAATTTTCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTAATCGCCCgg > 1:552729/1‑250 (MQ=255) gCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTTAGGCTCTG‑GGAGATTGCGC‑GAAAATTT‑TTTCCTTTTGGGCGGGTAAAAATTTCCTCAACCCCCCTGCCCTGG‑CCGACCCAACACTAATTAATccccctgcc > 2:461890/1‑245 (MQ=255) gCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGAt < 1:466595/249‑1 (MQ=255) cAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCg < 2:793288/250‑1 (MQ=255) aaaCGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCt < 1:741278/250‑1 (MQ=255) aaaCGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGTCTCTG‑GGAGATGCCGC‑GCAAAATT‑TTTCCTTTTGGGCGGGGAAAATTTTCATCCACTCTCCCGCGCTGG‑GAGACCCAACCTTTATTACTCGCCTGTccccccccc > 2:118842/1‑243 (MQ=255) aaCGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCtt < 2:478648/250‑1 (MQ=255) ctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGTGGGGCTTAAGATTATG‑GCAAAGGGCGC‑CAAAAATC‑TTTCCTTTTGCGCGGGGAAAATTTCTATCCACTCTCCTGCGCTGG‑CAGACCCACACTTAATTACTCGTCTGGCCCATCGCTTTGGCgcg > 2:762885/1‑246 (MQ=255) aagaagGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGGTAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTTATTACTCGCCTGGGCGATCGCTTTGGCGTGCAgtg > 1:614201/1‑250 (MQ=255) tGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTTAGGCTCTT‑GGAGAGGCCGC‑GAAAATTC‑TTTTCTTTTGCGCGGGGAAAATTTCCATTAACTCTCCTCCGCTGG‑CAGACCCACCATTAATTATCCGCCCGGCCCATCCCTTTTGCGGGCGgtggtttttgggc > 2:833544/1‑240 (MQ=255) tgtTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGTGGGGATTAAATATCTT‑GTAAAAGACGC‑GGAAATTT‑TTTTCTTTTTGGCGGATAAAAATTCCATTAACCCTCCGCCGCTGG‑CAGACCCAAAAATTATTACTCTCCCGGGCGATCGCTTTGTCGTGCAGTGGTTTTTTGTGGGTATTGATAc > 2:867367/1‑250 (MQ=255) tgtTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTT‑TTTCCTTTTGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTTGCGTGCAGTGTATTGTGGTCGGTATTGATAc > 1:880390/1‑250 (MQ=255) ttCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCt < 1:530726/250‑1 (MQ=255) cTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTTAGGCTCTG‑GGAGATGCCGC‑GAAAAATT‑TTTTCTTTTGCGCGGGTAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCCACATTAAATACTCCCCTGGCCGACCGCCTTTGCGTGGCGCGGATTGTGGTCGGGATTGGTACCTgg > 2:876538/1‑250 (MQ=255) cTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTg < 1:26901/249‑1 (MQ=255) tACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTTCTTTTGCGCGGATAAAATTTCCATCAAATCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTgg > 1:546605/1‑249 (MQ=255) cGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTG‑GGAGATGCCGC‑GGAAATTC‑TTTTCTTTGGCGCGGGTAAAATTTTCATCAACTCTCCTGCGCTGG‑CAGAACCAACATTAATTACTCGCCTGGCCGATCGCTTTTGCGGGGAGTGGATTGTGGTCGGTATTGATACCTGGTac > 1:607049/1‑250 (MQ=255) gATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCTTTTTGTGTGGCGTGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTacg < 1:871885/250‑1 (MQ=255) aCCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTTAGGCTCTG‑GAAAATGCCGC‑GGAAATTT‑TTTTCTTTTGCGCGGGTAAAATTTTCATCAACTCTCCTGCCCTGG‑CAGACCCAACATTAATTAATTCCCTGGGCCATCGCCTTTGCGGGCAGGGGATTTTGGTCCGGCTTTGTACCTGGGACGACGCCg > 2:416481/1‑250 (MQ=255) ccGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGCTCTG‑GAAGATGCCGC‑GCAAATTC‑TTTTCTTTTGCGCGGGTAAAATTTTCATCAACTCTCCTGCGCTGG‑GAGACCCCACATTAATTAATCGCCTCGCCGACCGCCTTGGCGTGCAGCGGATTTTGGTCGGGATTGTTACCTGGTACGGCGccc > 2:58901/1‑248 (MQ=255) cGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGaa < 2:159394/250‑1 (MQ=255) ccAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAAcc < 1:861320/248‑1 (MQ=255) ccAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCg < 1:675687/249‑1 (MQ=255) gCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTG‑GAAGATGCCGC‑GAAAAATC‑TTTTCTTTTGCGCGGATAAAATTTTCATCAAATCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCCGGCCGATCGCTTTGGCGGGCAGTGGATTTTGGGCGGGATTGATACCTGGGACGACGCCGAAACCGGTa > 1:449974/1‑249 (MQ=255) gATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTTAATGTCTGTGGACAT‑CGCC‑GCACATTC‑TTTCCTTTTGCGCGGGTAAAATTTCCCTCACCTCTCCTCCGCTGG‑CAGACCCACCATTATTTAATCTTCTCGCCGCTCTCTTTTGTGTGTAGTGTATTGTTGTGGGTATTTATTCCTTGTACGACGTCGATACCGGTAAat > 2:558073/1‑250 (MQ=255) aTGGCCATGTGGTAGATGAAAGCTGGGTATCTCTCGTGGCGGAAGTGATCTACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTATG‑GAAGATGCCGC‑GAAAAGTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCGGCGCTGG‑CATACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAata < 1:743895/250‑1 (MQ=255) cGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGCTCTG‑GGAGATGCCGC‑GAAAAATT‑TTTCCTTTGGCGCGGGGAAAAATTCCATCAACCCTCCCGCGCCGG‑CAGACCCCACATTAATTAATCGCCCGGCCGATCGCCTTGGCGGGCGGGGGATTGTGGTGGGGGTTTGTACCCGGGACGACGCCGGAACCCGGAAAAATAAtg > 2:268296/1‑250 (MQ=255) tgGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGa < 1:805982/249‑1 (MQ=255) caaggtggggTATCTCGCGTGGCTGAAGTCATCAACATTCCGTTTTGTGTGTCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTACCGTCAACTCGCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATAGCTGGTACAACGCCGAAACCGGTAAATATCATGTGAATCAAtata < 2:338874/243‑1 (MQ=255) aGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCg < 1:544334/250‑1 (MQ=255) tATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAATCTCTT‑GAAGATGCCGCGGAAAATTT‑TTTCTTTTGG‑GCGGGTAAAAATTTCATCAACTCTCCTGCGCTGG‑CAGAACCAACATTAATTACTCCACCTGCCGATCCCCTTGGCCTGCAGTGTATTGTGGTCGGTATTGATAACCGGGTCGGCGCCGAAAACGGGGAAAATCATGTGAATCAATATAATGGGGATg > 2:441421/1‑249 (MQ=255) ctcGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTTGCGCGGATAAAATTTTCATCAACTCTCCTGCGCTGG‑CAGACCCCACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGGATTGATACCTGGTACGGCGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAg > 1:752500/1‑250 (MQ=255) gcgTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTTGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGGACGACGCCGAAACCGGGAAATATCATGCGAATCAATATACCGGCGATGAAAGCCg > 1:53672/1‑250 (MQ=255) gCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCAccc < 2:100514/250‑1 (MQ=255) gCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCAcc < 1:113217/249‑1 (MQ=255) ggAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCcgc < 2:207463/250‑1 (MQ=255) ggAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAAATC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAAATCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGGAGGGTATTGTGGTCGGGATTGATAACTGGTACGACGCCGAAACCGGGAAAAATCATGTGAAACAATATACCGGCGATGAAAGCCGCACCcgc > 1:301077/1‑250 (MQ=255) gAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GGAGATGCCGC‑GAAAATTC‑TTTTCTTTTGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCCGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGGATTGATACCTGGTACGACGCCGAAACCGGTAAATATAATGTGAAACAATATACCGGCGAAGAAAGCCGCACCcgcg > 2:361046/1‑250 (MQ=255) gAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGGTAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTAATCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGGATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGGTGAAAACCGCACCcgcg > 1:659719/1‑250 (MQ=255) tGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTG‑GGAGATGCCCC‑GAAAAATT‑TTTTCTTTTGCGCGGGTAAAATTTTCATCAACTCTCCTGCGCTGG‑CAGACCCCACCTTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGGGGATTTTGGGCGGGATTGATACCTGGTACGACGCCGAAAACGGTAAATAACATGTGAATCAATATAACGGCGGTGAAAACCGCAcccccctccc > 2:821810/1‑243 (MQ=255) gACATTCCGTGTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAAt < 2:738264/250‑1 (MQ=255) cGTTTTGTGTGGCGGGTGGGATTTAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTTCTTTTGCGCGGGTAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCCACATTAATTACTCGCCTCGCCGATCGCTTTTGCGTGGAGTGTATTTTGGTCGGGATTGATACCTGGTACGACGCCGAAACCGGGAAATATCATGTGGATCAATATACCGGCGGTGAAAACCGCCCCCCCCTCACTCCATGGGaaa > 2:686572/1‑249 (MQ=255) cGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAAc < 1:207523/250‑1 (MQ=255) ttttGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTTCTTTTGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCCACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGc > 1:413780/1‑250 (MQ=255) tgGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACt < 1:890673/249‑1 (MQ=255) gTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTa > 1:22862/1‑248 (MQ=255) tGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAg < 1:312712/250‑1 (MQ=255) aTTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAg > 1:953619/1‑250 (MQ=255) | CTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTG‑GAAGATGCCGC‑GAAAATTC‑TTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGG‑CAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAG > NZ_CP009273/2089328‑2089820 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |