Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,036,3530CT46.8% 19.4 / 19.9 48F193F (TTC→TTT) srkAstress response kinase SrkA
Reads supporting (aligned to +/- strand):  ref base C (16/9);  new base T (22/0);  total (39/9)
Fisher's exact test for biased strand distribution p-value = 1.86e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.84e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTT  >  NZ_CP009273/4036110‑4036595
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    
ccAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTCACCGtt                                                                                                                                                                                                                                                >  1:681359/1‑250 (MQ=255)
   gTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTACCGTTCTg                                                                                                                                                                                                                                             >  2:828199/1‑250 (MQ=255)
         aGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGGATTCACCGTTcgtcgggca                                                                                                                                                                                                                                       >  2:563208/1‑242 (MQ=255)
           cTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGAGTTTACCGGTCTTCGGcctca                                                                                                                                                                                                                                     >  1:39085/1‑246 (MQ=255)
                   atCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACCTGGAGACt                                                                                                                                                                                                                             >  1:771805/1‑250 (MQ=255)
                       aTCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCa                                                                                                                                                                                                                         <  2:89326/250‑1 (MQ=255)
                            aTGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCCTTCTTCGGCGACATGGAGACCGCCCCCCCg                                                                                                                                                                                                                    >  2:961839/1‑250 (MQ=255)
                                aaGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAGAATTTTACCGTTCTGCGGCTGCATGGAGACTGGCCCGCCGGGa                                                                                                                                                                                                                 >  2:838532/1‑249 (MQ=255)
                                  gCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGGGACTGCCCCCCCGGGGata                                                                                                                                                                                                              >  1:102841/1‑250 (MQ=255)
                                   cGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGatat                                                                                                                                                                                                             >  1:88683/1‑250 (MQ=255)
                                            gTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCCGGGAAATTCTCTGgcg                                                                                                                                                                                                    >  1:503355/1‑250 (MQ=255)
                                                                cAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTttgttg                                                                                                                                                                                >  1:274438/1‑250 (MQ=255)
                                                                    cGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGGGACTGCCACGCCGGGGATATTTTCTGGCGCGGTGGTCCAATGTTTGTTGATCt                                                                                                                                                                            >  1:746601/1‑250 (MQ=255)
                                                                     ggggCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAAATTTTAACGTTCTGCGGCGACATGGAGAATGCCCCCCCGGGGATATTTTCTGGGGCGGTGGGCCAATGTTTGTTGATCTg                                                                                                                                                                           >  2:567692/1‑250 (MQ=255)
                                                                                 gCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAGGAGTTAACAGGTCTGCGGCGACATGGAGGCTGACCCGGCGGGAGTAATCTCTTGGGCGGTGGTCCAATGTTTGTTGTTCTGGGTTGTGCACg                                                                                                                                                                >  2:435338/1‑249 (MQ=255)
                                                                                     tttATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGGGACTGCCACGCCGGGGATATTTTCTTGCGCGGTGGGCCAATGTTTGTTGATCTGGATGATGCACGTAATg                                                                                                                                                           >  1:223164/1‑250 (MQ=255)
                                                                                        aTCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGt                                                                                                                                                         <  2:966016/249‑1 (MQ=255)
                                                                                              cGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGAATGCCACGCCGGGGATATTCTCTGGCGCGCTGGTCCACTGTTTGTTGGTCTGGGTGATGCACGGAATGGTCCAGCCg                                                                                                                                                  >  1:370646/1‑250 (MQ=255)
                                                                                               gCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTAACGCTCTGCGGCTACATGGAGACCGCCACCCCGGGGATATTCTCTTGCGCGATGGTCCCATGTTTGTTGATCTGGGTGATGGACGTAATGGTCCAGCCGt                                                                                                                                                 >  1:258212/1‑250 (MQ=255)
                                                                                                cccGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGATGGGCCCATGTTTGTTGATCTGGGTGATGCACGTAATGGTCCAGCCGtt                                                                                                                                                >  1:378466/1‑250 (MQ=255)
                                                                                                      cATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATCCCTTCCGGGTTGAAAGCGGCATTCCGGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGAt                                                                                                                                          <  2:762587/250‑1 (MQ=255)
                                                                                                          ggTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGGGACTGCCACGCCGGGGATATTTTCCGGCGCGGTGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCCGGGTTtgt                                                                                                                                      >  1:181726/1‑250 (MQ=255)
                                                                                                                  cGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACCCTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACATATGGGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTg                                                                                                                              <  2:672052/250‑1 (MQ=255)
                                                                                                                     atatCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGGGACTGCCACGCCGGGGATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGGTGATGCACGTAATGGGCCAGCCGCTCAGGATTTGTGGATGTTTCt                                                                                                                            >  1:26309/1‑249 (MQ=255)
                                                                                                                       atCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTTTGCGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTGGCGCGATGGTCCCATGTTTGTTGATTTGGGTGATGCACGGAATGGTCCAGCCGTTCAGGGTTTTTGGGTGTTGCTCaa                                                                                                                         >  1:836395/1‑250 (MQ=255)
                                                                                                                            aTTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTTCAGCCGTTCAGGATTTGTGGATGTTGCTCAAt                                                                                                                        <  1:390019/246‑1 (MQ=255)
                                                                                                                            aTTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGc                                                                                                                     <  1:435831/249‑1 (MQ=255)
                                                                                                                             ttGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCCCGCCGGGGATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGGTGTTGCTCAATTGCGa                                                                                                                   >  1:792704/1‑250 (MQ=255)
                                                                                                                               gAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTTCGGCCATATGGAGACAGGCCCGCCGGGGATATTCTTTTGCGCGGGGGACCAATGTTTGTTGATCTGGGTGGTGTATGGAATGGTCCTACCGTTCAGGATTTTTGGGTGGTGCTCAATTGCggt                                                                                                                  >  2:253947/1‑247 (MQ=255)
                                                                                                                                   cACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTTTCTGGCGCGATGGTCCCATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGGTGCTCAATGGGGATAAAGc                                                                                                             >  1:125375/1‑250 (MQ=255)
                                                                                                                                        aaGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTTTCTGGCGCGATGGGCCCATGTTTGTTGATCTGGATGATGCACGGAATGGTCCAGCCGGTCAGGGTTTGTGGGTGTTGCTCAATGGCGGTAAAACCGag                                                                                                         >  1:731222/1‑249 (MQ=255)
                                                                                                                                         aGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACAAGGAGACTGCCACGGCGGGGAAATTTTCTGGCGCGATGGGTCAATGTTTTGTGTTCTGGATGATGCACGTAATGGGCCAGGCGGTTTAGATTTGTGGGTGTTGGTCAATGGCGGTAAAGACGag                                                                                                         >  2:702302/1‑248 (MQ=255)
                                                                                                                                              tttGGGGACGCTACTCTGATACTTTCCGGGTTTAAGGCGGCTTTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGag‑cagctg                                                                                                  <  2:979897/249‑3 (MQ=255)
                                                                                                                                                tGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGAATTTACCGTTCTGCGGCGACATGGAGACTGCCCCGCCCGGGATATTCTCTGGCGCGGTGGTCCCATGTTTGTTTTTCTGGGTGATGTACGTAATGGTCCAGCCGTTCAGGGTTTGTGGGGGTTGCTCAATGGGGGTAAAAACGGG‑GAGCggggg                                                                                               >  2:570978/1‑247 (MQ=255)
                                                                                                                                                         tACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGGGACTGCCACGCCGGGGATATTCTCTTGCGCGATGGTCCAATGTTTGTTGTTCTGGATGATGCACGTAATGGTCCAGCCGTTCTGGGTTTGTGGGTGTTGCTCAAAGGCGATAAAGCCGAG‑GCGCGGGTGCAAA‑Tggg                                                                                      >  2:87863/1‑248 (MQ=255)
                                                                                                                                                            acTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGAACGCCACGCCGGGGATATTTTCTGGGGCGGTGGGCCAATGTTTGGTTATCTGGATGATGCACGGAATGGGCCAGGCGGTTCGGGTTTGTGGGTGTTGGTCAATTGGGGTAAAAGCGAG‑CAGCGGGTGGAAC‑TGGgaaa                                                                                   >  2:263970/1‑248 (MQ=255)
                                                                                                                                                                 tACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCCCGCCGGGGATATTCTCTTGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGGAATGGTCCAGCCCGTCAGGGTTTTTGGGTGTTGCTCAATGGCGGTAAAACCGAG‑CAGGGGGTGGCAA‑TGGGAAAtattat                                                                             >  1:112598/1‑250 (MQ=255)
                                                                                                                                                                           gTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCCCGCCGGGAATATTCTCTGGCGCGATGGGCCAATGTTTGTTGATCTGGGTGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGGTGGTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAA‑TGGGAACTATTATTGAAGCtttt                                                                   >  2:215083/1‑248 (MQ=255)
                                                                                                                                                                           gTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCCTCATGGAGAATGCCACGCCGGGGATATTTTCTTGCGCGGTGGTTCACTGTTTGTTGGTTTGGGTGATGCACGTAATGGTTCAGCCCGTCCGGGATTGTGGGTGTTGGTCAATGGCGATAAAACCCAG‑CAACGGGTGGAAA‑TGGTAACTATTTTTTAAGCGTAt                                                                   >  2:390220/1‑250 (MQ=255)
                                                                                                                                                                            ttGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAAATATTATTGAAGCTTAt                                                                   >  1:410658/1‑249 (MQ=255)
                                                                                                                                                                              gAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTACCGTTCTTCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTTGCGCGATGGTCCCATGTTTTTTGGTCTGGGTGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGGTGCAAC‑TGGGAACTAATATTTaaagttttga                                                                 >  2:301293/1‑241 (MQ=255)
                                                                                                                                                                               aaaGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATgaag                                                               <  2:925497/250‑1 (MQ=255)
                                                                                                                                                                                         tCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCCGTCTTCGGCCCCCTGGAGACCGCCACGCCCGGGATATTTTCTCGCGCGGTGGGCCCATGTTTGTTGGTCTGGGTGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGGTGTTGGTCAAAGGCGATAAAAACCAG‑CAGCGGGTGGAAA‑TGGGAACTATTTTTGTAGGTTATTAAAAATTTAga                                                       >  2:800421/1‑247 (MQ=255)
                                                                                                                                                                                                    aCAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCCCGCCCGGGATATTCTCTTGCGCGATGGGCCCATGTTTGTTGATCTTGATGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGATGTTGCTCAATGGCGGTAAAACCGAG‑CAGCGGGTGGAAC‑TGGAAACTATTTTTTAAGCTTATGAAGAATTTTGCGAGTTCGACACCg                                          >  2:900004/1‑250 (MQ=255)
                                                                                                                                                                                                        aTGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTTCCCGTCTTCGGCTTCATGGAGACTTCCACCCCGGGAATATTCTTTTGGGCGGTGGGCCCATGTTTGTTGATCTGGGTTATGCACCTAATGGGCCTGGCGGTTAGGGTTTTTGGGTGTTTCTCAATGGGGAAAAAGACCCG‑GAACCGCTGCAACATGGAACTTATTTTTGAAG‑TTATGAAGGAATTTGGGGGGTCGGCACCGCtt                                       >  2:964021/1‑248 (MQ=255)
                                                                                                                                                                                                           aGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGGGACTGCCACGCCGGGGATATTTTCTGGCGCGATGGTCCCATGTTTTTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGGGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTTAAGCTTATTAAGAATTTAGCGAGTTCGACACCGCTGAAAt                                   >  1:742105/1‑250 (MQ=255)
                                                                                                                                                                                                                      gccgTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGGGAATGCCCCGCCGGGGATATTCTCTGGCGCGGTGGTCCAATGTTTGTTGGTCTGGAGGATGGACGTAATGGTCCAGCCGCTCAGGATTTTTGGGTGTTTCTCCATGGCGGTCAAACCGAGCCGGGGGAGTGAAC‑GGGAAA‑TATTTTTTAAGCTTATGAAGGATTTAGCGGGGTTGACAACGCTGAAAACgggaggatt                         >  2:764218/1‑242 (MQ=255)
                                                                                                                                                                                                                                            aaGCTTTCACGGTTCGGCGGCTACATGGAGACTGCCACGGCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGttt  <  2:820637/250‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    
CCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTT  >  NZ_CP009273/4036110‑4036595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑