Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,036,390	0	A	G	50.0%	50.2 / 18.5	52	N206D (AAT→GAT)	srkA	stress response kinase SrkA
Reads supporting (aligned to +/- strand):  ref base A (13/13);  new base G (26/0);  total (39/13)
Fisher's exact test for biased strand distribution p-value = 3.28e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGAT  >  NZ_CP009273/4036142‑4036635                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                        aaGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAGAATTTTACCGTTCTGCGGCTGCATGGAGACTGGCCCGCCGGGa                                                                                                                                                                                                                                                         >  2:838532/1‑249 (MQ=255)   gCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGGGACTGCCCCCCCGGGGata                                                                                                                                                                                                                                                      >  1:102841/1‑250 (MQ=255)    cGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGatat                                                                                                                                                                                                                                                     >  1:88683/1‑250 (MQ=255)             gTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCCGGGAAATTCTCTGgcg                                                                                                                                                                                                                                            >  1:503355/1‑250 (MQ=255)                                 cAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTttgttg                                                                                                                                                                                                                        >  1:274438/1‑250 (MQ=255)                                     cGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGGGACTGCCACGCCGGGGATATTTTCTGGCGCGGTGGTCCAATGTTTGTTGATCt                                                                                                                                                                                                                    >  1:746601/1‑250 (MQ=255)                                      ggggCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAAATTTTAACGTTCTGCGGCGACATGGAGAATGCCCCCCCGGGGATATTTTCTGGGGCGGTGGGCCAATGTTTGTTGATCTg                                                                                                                                                                                                                   >  2:567692/1‑250 (MQ=255)                                                  gCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAGGAGTTAACAGGTCTGCGGCGACATGGAGGCTGACCCGGCGGGAGTAATCTCTTGGGCGGTGGTCCAATGTTTGTTGTTCTGGGTTGTGCACg                                                                                                                                                                                                        >  2:435338/1‑249 (MQ=255)                                                      tttATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGGGACTGCCACGCCGGGGATATTTTCTTGCGCGGTGGGCCAATGTTTGTTGATCTGGATGATGCACGTAATg                                                                                                                                                                                                   >  1:223164/1‑250 (MQ=255)                                                         aTCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGt                                                                                                                                                                                                 <  2:966016/249‑1 (MQ=255)                                                               cGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGAATGCCACGCCGGGGATATTCTCTGGCGCGCTGGTCCACTGTTTGTTGGTCTGGGTGATGCACGGAATGGTCCAGCCg                                                                                                                                                                                          >  1:370646/1‑250 (MQ=255)                                                                gCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTAACGCTCTGCGGCTACATGGAGACCGCCACCCCGGGGATATTCTCTTGCGCGATGGTCCCATGTTTGTTGATCTGGGTGATGGACGTAATGGTCCAGCCGt                                                                                                                                                                                         >  1:258212/1‑250 (MQ=255)                                                                 cccGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGATGGGCCCATGTTTGTTGATCTGGGTGATGCACGTAATGGTCCAGCCGtt                                                                                                                                                                                        >  1:378466/1‑250 (MQ=255)                                                                       cATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATCCCTTCCGGGTTGAAAGCGGCATTCCGGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGAt                                                                                                                                                                                  <  2:762587/250‑1 (MQ=255)                                                                           ggTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGGGACTGCCACGCCGGGGATATTTTCCGGCGCGGTGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCCGGGTTtgt                                                                                                                                                                              >  1:181726/1‑250 (MQ=255)                                                                                   cGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACCCTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACATATGGGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTg                                                                                                                                                                      <  2:672052/250‑1 (MQ=255)                                                                                      atatCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGGGACTGCCACGCCGGGGATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGGTGATGCACGTAATGGGCCAGCCGCTCAGGATTTGTGGATGTTTCt                                                                                                                                                                    >  1:26309/1‑249 (MQ=255)                                                                                        atCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTTTGCGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTGGCGCGATGGTCCCATGTTTGTTGATTTGGGTGATGCACGGAATGGTCCAGCCGTTCAGGGTTTTTGGGTGTTGCTCaa                                                                                                                                                                 >  1:836395/1‑250 (MQ=255)                                                                                             aTTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTTCAGCCGTTCAGGATTTGTGGATGTTGCTCAAt                                                                                                                                                                <  1:390019/246‑1 (MQ=255)                                                                                             aTTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGc                                                                                                                                                             <  1:435831/249‑1 (MQ=255)                                                                                              ttGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCCCGCCGGGGATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGGTGTTGCTCAATTGCGa                                                                                                                                                           >  1:792704/1‑250 (MQ=255)                                                                                                gAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTTCGGCCATATGGAGACAGGCCCGCCGGGGATATTCTTTTGCGCGGGGGACCAATGTTTGTTGATCTGGGTGGTGTATGGAATGGTCCTACCGTTCAGGATTTTTGGGTGGTGCTCAATTGCggt                                                                                                                                                          >  2:253947/1‑247 (MQ=255)                                                                                                    cACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTTTCTGGCGCGATGGTCCCATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGGTGCTCAATGGGGATAAAGc                                                                                                                                                     >  1:125375/1‑250 (MQ=255)                                                                                                         aaGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTTTCTGGCGCGATGGGCCCATGTTTGTTGATCTGGATGATGCACGGAATGGTCCAGCCGGTCAGGGTTTGTGGGTGTTGCTCAATGGCGGTAAAACCGag                                                                                                                                                 >  1:731222/1‑249 (MQ=255)                                                                                                          aGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACAAGGAGACTGCCACGGCGGGGAAATTTTCTGGCGCGATGGGTCAATGTTTTGTGTTCTGGATGATGCACGTAATGGGCCAGGCGGTTTAGATTTGTGGGTGTTGGTCAATGGCGGTAAAGACGag                                                                                                                                                 >  2:702302/1‑248 (MQ=255)                                                                                                               tttGGGGACGCTACTCTGATACTTTCCGGGTTTAAGGCGGCTTTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGag‑cagctg                                                                                                                                          <  2:979897/249‑3 (MQ=255)                                                                                                                 tGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGAATTTACCGTTCTGCGGCGACATGGAGACTGCCCCGCCCGGGATATTCTCTGGCGCGGTGGTCCCATGTTTGTTTTTCTGGGTGATGTACGTAATGGTCCAGCCGTTCAGGGTTTGTGGGGGTTGCTCAATGGGGGTAAAAACGGG‑GAGCggggg                                                                                                                                       >  2:570978/1‑247 (MQ=255)                                                                                                                          tACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGGGACTGCCACGCCGGGGATATTCTCTTGCGCGATGGTCCAATGTTTGTTGTTCTGGATGATGCACGTAATGGTCCAGCCGTTCTGGGTTTGTGGGTGTTGCTCAAAGGCGATAAAGCCGAG‑GCGCGGGTGCAAA‑Tggg                                                                                                                              >  2:87863/1‑248 (MQ=255)                                                                                                                             acTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGAACGCCACGCCGGGGATATTTTCTGGGGCGGTGGGCCAATGTTTGGTTATCTGGATGATGCACGGAATGGGCCAGGCGGTTCGGGTTTGTGGGTGTTGGTCAATTGGGGTAAAAGCGAG‑CAGCGGGTGGAAC‑TGGgaaa                                                                                                                           >  2:263970/1‑248 (MQ=255)                                                                                                                                  tACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCCCGCCGGGGATATTCTCTTGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGGAATGGTCCAGCCCGTCAGGGTTTTTGGGTGTTGCTCAATGGCGGTAAAACCGAG‑CAGGGGGTGGCAA‑TGGGAAAtattat                                                                                                                     >  1:112598/1‑250 (MQ=255)                                                                                                                                            gTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCCCGCCGGGAATATTCTCTGGCGCGATGGGCCAATGTTTGTTGATCTGGGTGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGGTGGTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAA‑TGGGAACTATTATTGAAGCtttt                                                                                                           >  2:215083/1‑248 (MQ=255)                                                                                                                                            gTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCCTCATGGAGAATGCCACGCCGGGGATATTTTCTTGCGCGGTGGTTCACTGTTTGTTGGTTTGGGTGATGCACGTAATGGTTCAGCCCGTCCGGGATTGTGGGTGTTGGTCAATGGCGATAAAACCCAG‑CAACGGGTGGAAA‑TGGTAACTATTTTTTAAGCGTAt                                                                                                           >  2:390220/1‑250 (MQ=255)                                                                                                                                             ttGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAAATATTATTGAAGCTTAt                                                                                                           >  1:410658/1‑249 (MQ=255)                                                                                                                                               gAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTACCGTTCTTCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTTGCGCGATGGTCCCATGTTTTTTGGTCTGGGTGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGGTGCAAC‑TGGGAACTAATATTTaaagttttga                                                                                                         >  2:301293/1‑241 (MQ=255)                                                                                                                                                aaaGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATgaag                                                                                                       <  2:925497/250‑1 (MQ=255)                                                                                                                                                          tCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCCGTCTTCGGCCCCCTGGAGACCGCCACGCCCGGGATATTTTCTCGCGCGGTGGGCCCATGTTTGTTGGTCTGGGTGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGGTGTTGGTCAAAGGCGATAAAAACCAG‑CAGCGGGTGGAAA‑TGGGAACTATTTTTGTAGGTTATTAAAAATTTAga                                                                                               >  2:800421/1‑247 (MQ=255)                                                                                                                                                                     aCAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCCCGCCCGGGATATTCTCTTGCGCGATGGGCCCATGTTTGTTGATCTTGATGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGATGTTGCTCAATGGCGGTAAAACCGAG‑CAGCGGGTGGAAC‑TGGAAACTATTTTTTAAGCTTATGAAGAATTTTGCGAGTTCGACACCg                                                                                  >  2:900004/1‑250 (MQ=255)                                                                                                                                                                         aTGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTTCCCGTCTTCGGCTTCATGGAGACTTCCACCCCGGGAATATTCTTTTGGGCGGTGGGCCCATGTTTGTTGATCTGGGTTATGCACCTAATGGGCCTGGCGGTTAGGGTTTTTGGGTGTTTCTCAATGGGGAAAAAGACCCG‑GAACCGCTGCAACATGGAACTTATTTTTGAAG‑TTATGAAGGAATTTGGGGGGTCGGCACCGCtt                                                                               >  2:964021/1‑248 (MQ=255)                                                                                                                                                                            aGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGGGACTGCCACGCCGGGGATATTTTCTGGCGCGATGGTCCCATGTTTTTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGGGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTTAAGCTTATTAAGAATTTAGCGAGTTCGACACCGCTGAAAt                                                                           >  1:742105/1‑250 (MQ=255)                                                                                                                                                                                       gccgTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGGGAATGCCCCGCCGGGGATATTCTCTGGCGCGGTGGTCCAATGTTTGTTGGTCTGGAGGATGGACGTAATGGTCCAGCCGCTCAGGATTTTTGGGTGTTTCTCCATGGCGGTCAAACCGAGCCGGGGGAGTGAAC‑GGGAAA‑TATTTTTTAAGCTTATGAAGGATTTAGCGGGGTTGACAACGCTGAAAACgggaggatt                                                                 >  2:764218/1‑242 (MQ=255)                                                                                                                                                                                                             aaGCTTTCACGGTTCGGCGGCTACATGGAGACTGCCACGGCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGttt                                          <  2:820637/250‑1 (MQ=255)                                                                                                                                                                                                                      ccGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTttatta                                 >  1:944585/1‑250 (MQ=255)                                                                                                                                                                                                                          tCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCtt                             >  1:785788/1‑250 (MQ=255)                                                                                                                                                                                                                           cTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGAGTGAACCTGAACGCCCCATGCGTTTGGTTTATTATCTTg                            >  1:372791/1‑250 (MQ=255)                                                                                                                                                                                                                               gtcTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTg                        <  2:65982/248‑1 (MQ=255)                                                                                                                                                                                                                                 cTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGc                      >  1:297737/1‑250 (MQ=255)                                                                                                                                                                                                                                  tACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGc                      >  1:45772/1‑249 (MQ=255)                                                                                                                                                                                                                                    cATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTa                    <  1:563033/249‑1 (MQ=255)                                                                                                                                                                                                                                        gagaCTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATgc                <  1:391595/249‑1 (MQ=255)                                                                                                                                                                                                                                              gCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTg         >  1:618322/1‑250 (MQ=255)                                                                                                                                                                                                                                               ccACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTgg        >  2:219150/1‑250 (MQ=255)                                                                                                                                                                                                                                                 aCGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGc      <  1:828199/250‑1 (MQ=255)                                                                                                                                                                                                                                                     cGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGAt  <  2:102841/250‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                        AAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG‑CAGCGGATGCAAC‑TGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGAT  >  NZ_CP009273/4036142‑4036635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑