Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,145,154	0	T	G	46.0%	58.7 / 20.9	54	intergenic (+27/+38)	yegJ/pphC	DUF2314 domain‑containing protein/protein‑serine/threonine phosphatase PphC
Reads supporting (aligned to +/- strand):  ref base T (19/8);  new base G (0/23);  total (19/35)
Fisher's exact test for biased strand distribution p-value = 1.03e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACC‑TATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCG  >  NZ_CP009273/2144906‑2145400                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                           gTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGATATTACAATTATTCTTTTTTTGTTACC‑TTTCCCAAAAAAAAAAACGACGCGACAAGAGGAAAAATAATTTGCTTATCAGCCGCCTTATTTTTTT‑TTTTAA‑A‑Tg                                                                                                                                                                                                                                                           >  1:537911/1‑250 (MQ=255)    aCTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACC‑TATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGc                                                                                                                                                                                                                                                         <  2:239637/249‑1 (MQ=255)    aCTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACC‑TATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGc                                                                                                                                                                                                                                                         <  2:465950/249‑1 (MQ=255)         ttCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACC‑TATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTGa                                                                                                                                                                                                                                                  <  2:498889/250‑1 (MQ=255)              aCGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACC‑TATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑ttg                                                                                                                                                                                                                                            <  1:380314/249‑1 (MQ=255)                    tGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACC‑TATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTGTCtt                                                                                                                                                                                                                                     <  1:32587/250‑1 (MQ=255)                      tttATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACC‑TATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTGTCTT‑Ca                                                                                                                                                                                                                                  <  2:751944/250‑1 (MQ=255)                                            ccctgggTATTGTAAAAAAGTAAAAATAGGGCAGGGAGGGAAAAATAAAAAAAAAAAATTTTTCAGACTGGATTTTTCCGCGGGGGAAAAAATTTTAGGGGGTTTATCCTTTTGTTCTTTTATTGGCCCCC‑TACCAAAAAAAAGAAGCCGACGAGTAAAGAAAAAAATTATTTCGCTAACCAGCCTTTATATTTTTTT‑TTAAAA‑C‑GGGCGG‑TTGA‑T‑TTTTTGTCTT‑CAACCGCCATGAGAAACTCActct                                                                                                                                                                                                            <  2:312159/245‑1 (MQ=255)                                                      tgtaaaaaccgaaGAATATGGCCGGGAGGGAAAAATAAAAAAAGAAGATTTTGCAGGCTGGATGTATCCGCGGGGGAAAAAAATTTACGGCGGTTATCCCATTGATCCTTTATTGGTCACC‑TACCAAAAAAAAGAAGCCGACGAGCTAAGAGAAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTAAAA‑C‑GGGCGG‑TTGA‑A‑TTGTTGTCTT‑AAACCGCCATGAGAAACTCACTCTGCCCACAgc                                                                                                                                                                                                   <  1:359684/237‑1 (MQ=255)                                                       gTTACAAACGTAGAATATGGCAAGGAGTGAAAAATAAAGAAAGAAGATATTCCAGACTGAATGTATACGCGGGGGAATAAAATTTACGGCGTTTATCCCTTTGACCCTTTATTGGTCCCT‑TATCAAAAAGAAGAAGCCGACGAGCAAAGAGAAAAATTATTTCGCTAATCAGCCTTTATATTTTTTT‑TTTAAC‑C‑GGGCGG‑TTGA‑T‑TTGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAgcg                                                                                                                                                                                                  <  1:362235/249‑1 (MQ=255)                                                       gTTAAAAAAGAAGAATAGGGCAAGGAGTGAAAAAAAAAAAAAGAAAATATTTCAGACTGGTTGTATCCGGGGGGGAAAAAATTTTACGGGGTTTATCCCTTTGTTCTTTTATTGTCCCCC‑TACCAAAAAAAAGAAGCCGACGAGCAAAGAAAAAATTTATTTCGTAAACCAGCCTTTTTTTTTTTTT‑TTAAAC‑C‑GGGCGG‑TTAA‑T‑TTGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAgcg                                                                                                                                                                                                  <  2:785923/249‑1 (MQ=255)                                                             aaCGTAAAATATGGCAAGGAGTGAAAAATAAAGAAAGAAGATATTTCAGACTGGTTGTATACGCGGGGGGAAAAATTTTCCGGGGTTTATCCCTTTGATCTTTTATTGGTCCCC‑TATCAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTATTTCGCTAATCAGCCTTTATATTTATTG‑TTAAAA‑C‑GGGCGT‑TTAA‑A‑TTGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGc                                                                                                                                                                                           <  1:563703/250‑1 (MQ=255)                                                                           caaGGAGTGAAAAATAAAAAAAAAAAATATTTGAGACGGGATGTAAACGCGGGGGAAAAAATTTTACGGCGTTAATCCCTTTGTTCTTTTATGGGTCCCC‑TACCAAAAAGAAGAGCCCGAGGAGCAAAGAAAAAAATTAGTTCACTAGTCCGCCGTTATTTTTATTT‑TAAAAC‑C‑GGGCGG‑TAAA‑T‑TTGTTGTCTC‑AAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcat                                                                                                                                                                             <  2:90186/248‑1 (MQ=255)                                                                                          aaaaaaaaaaGATATTTCAGACTGGATGTATACGCGCGGGAAAAAAATTTACGGCGTTTATACCATTGATCTTTTATTGGTCCCC‑TACCAAAAAAAAGAAGCCGACGACCAAAGAGAAAAATTAGTTCCCTAATCAGCCGTTATATTTTTTG‑TTAAAA‑C‑GGGCGG‑TTGA‑A‑TTGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGt                                                                                                                                                              <  1:619421/246‑1 (MQ=255)                                                                                                 gaataTATTTAAGACTGGTTGTATACGCGGGGGAAAAAAATTTACGGGGGTTAACCCATTGATCTTTTATTGGTCCCC‑TACCAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTAGTTCGTTAATCAGCCTTTATATTTTTTG‑TTAAAA‑C‑GGGCGG‑TTAA‑A‑TTGTTGTCTT‑AAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTAccgc                                                                                                                                                       <  1:259583/250‑1 (MQ=255)                                                                                                           catatgggATGTATCCGGGGGGGAAAAAATTTTGCGGCTGTTATACTTTTGATCCTTTATTGGTCCCC‑TATCAAAAAGAAGAAGCCGAGGAGAAAAAAAAAAAATTTTTCCGATAATCAGCTTTTTTTTTTATT‑‑TTTAAA‑CGGGGCGT‑TTGA‑T‑TTTTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGa                                                                                                                                              <  2:651283/243‑1 (MQ=255)                                                                                                           cAGACTGGATGTATACGCGGGGGGATAAAATTTACGGCGTTTATACCTTTGATCTTTTA‑TGGTCCCCTTATCAAAAAAAAGAAGCCGACGAGAAAAGAGAAAAATTATTTCGCTAACCACCCTTTATATTTATTT‑TTTAAA‑C‑GGGCGG‑TTGA‑A‑TTGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGa                                                                                                                                              <  1:126682/249‑1 (MQ=255)                                                                                                               cTGGTTTTATCCGCGGGGGAAAAAATTTTACGGGGTTAATCCCTTTGACCTTTTATGGGCCCCC‑TATCAAAAAAAAGAAGCCGACGAGAAAAGAGAAAATTTAGTCCGCAAATCAGCCTTTTTTTTTTTTT‑TTAAAA‑C‑GGGGGT‑TTAA‑T‑TTTTTGTCTT‑AAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaaaa                                                                                                                                         <  1:567777/250‑1 (MQ=255)                                                                                                                         aggggagggaaaaaatTTTACGGGGTTTTTACCATTGTTCTTTTATTGGTCCCC‑TAACAAAAAGAAGAACCCGAGGATAAAAAAGAAAAATTGTTCCCCTAATCAGCTTTTAT‑TTTTTTT‑TTTAAA‑C‑CGGGGGTTTGA‑T‑TTGTTTTCTC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTaa                                                                                                                               <  2:749054/238‑1 (MQ=255)                                                                                                                          cgcgcgGGAATAAATTTTACGGCGGTTATACCTTTGATCTTTTATTGGTCACC‑TACAAAAAAGAAGAAGCCGACGAGCAAAGAAAAAAATTATTTCCCTAATCAGCCTTTATATTTTTTT‑TTTAAC‑C‑GGGCGT‑TTAA‑T‑TTTTTTTCTT‑AAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTaa                                                                                                                               <  1:914711/249‑1 (MQ=255)                                                                                                                              ggggaaaaaaaTTTACGGGGGTTATCCCATTGTCCTTTTATTGGTCCCT‑TAACAAAAAAAAGAACCCGACGAGCAAAGAGAAAAATTATTTCGCTAACCAGCCTTTATTTTTATTT‑TTTAAC‑C‑GGGCGG‑TTAA‑T‑TTTTTTTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAAtt                                                                                                                          <  1:686158/243‑1 (MQ=255)                                                                                                                                        ctttACGGCTTTTATCCCTTTTTTCTTTTATTGTTCACC‑TCCCAAAAAAAAAAACCCGACGAGAAAAGAAAAAATTTAGTTCGTTACTCA‑CCTTTTTTTTTTTTT‑TTAAAA‑C‑CGGGGG‑TTTA‑ATTTTTTGTCTA‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaataa                                                                                                                <  2:316305/249‑1 (MQ=255)                                                                                                                                               gcggTTATACCATTGATCCTTTATTGGTCACC‑TATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTTA‑A‑TTTTTGTCTT‑TAACCCCCATGAGAAAATCACCCTTCCCCCAGCGCCCGTGCCAACGTTTTTTCATCATCCCTCAGCCCCTTTATCGCCCGGCTGGGCAAAAACTTCTTTTACAATTCAGGTAATaaaatgagg                                                                                                         >  2:246490/1‑244 (MQ=255)                                                                                                                                               gcggTTATACCATTGATCCTTTATTGGTCACC‑TATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTTTTTTCTT‑CACCCCCCCTGAGAAAAACAATCTGCCCACAGCGCCAGTGCCCACGGTTTTTCCTCATCCCCACGCCCCCTTACCCCCCGGCTGGGCAAAAACTCCCTTTACAAATCAGGGAATAAAACCAgt                                                                                                         >  2:653923/1‑250 (MQ=255)                                                                                                                                                              aTCTTTTATTGTCAACC‑TATCAAAAAAAAGAAGCCGACGAGCAAAGAAAAAATTTAGTTCGTTAATCATCTTTTATTTTTTTTT‑TTAAAC‑C‑GGGCGG‑TTAA‑T‑TTTTTTTCTC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgc                                                                                           <  2:899150/249‑1 (MQ=255)                                                                                                                                                                ccTTTATTGGTCACC‑TATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAACGCCCGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgctgc                                                                                        >  1:838392/1‑250 (MQ=255)                                                                                                                                                                       tGGTCACC‑TATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTGTTTT‑CAAACGCCATGAGAAAATCAATCTGCCCACAGCGCCAGGGGCAACGGTTTATCATCATCCGTACGCTCGGTTACCGCCGGGCTGGACAAAAAATGCTTTAACAAATCAGGGAATAAAAAGAGGTGTTCCTGCGTTGCTGCCGCCAgg                                                                                 >  1:697719/1‑249 (MQ=255)                                                                                                                                                                         ggcccccaaccaaaaaaaaaaaGCCGAGGAGAAAAGAAAAAATTTAGTCCGCAAACCACCTTTTAT‑TTTTTTT‑TTTAAA‑C‑CGGGGG‑TTTA‑ATTTTTTTTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGt                                                                                 <  1:212791/237‑1 (MQ=255)                                                                                                                                                                            cccttATCAAAAAAAAAAAGCCGAGGAGTAAAGAAAAAAATTAGTCCGTTAATCGGCGTTTATTTTTTTTT‑TTAAAC‑C‑GGGGGG‑TTAA‑A‑TTGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCAt                                                                             <  1:528583/246‑1 (MQ=255)                                                                                                                                                                            cccctcccaaaaaaaaaaaCCCGACGAAAAAAGAAAAAATTTGGTTCGTTAACCAGCTTTTATTTTTTTTT‑TTAAAA‑C‑GGGCGT‑TTAA‑T‑TTGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCAt                                                                             <  1:109587/241‑1 (MQ=255)                                                                                                                                                                                 aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTGTCTT‑CAAACGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATTATCATCCGTACGCCCGTTTACCGCCGGGCTGGACAAAAAATGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTTaa                                                                         >  2:617022/1‑249 (MQ=255)                                                                                                                                                                                          aagaagCCGAAGAACAAAGAGAAAAATTAGTTCGTAAATCAGCGGTTATTTTTATTG‑TTAAAA‑C‑GGGCGG‑TTAA‑T‑TTGTTTTCTT‑AAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTa                                                               <  1:486087/250‑1 (MQ=255)                                                                                                                                                                                                 gaaggagaaaaGAGAAAGTTTAGTTCGTAACTCACTCTTTATATTTTTTT‑GTTAAC‑G‑GGGCGT‑TTGA‑T‑TTGTTTTCAC‑AACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTaaagaaag                                                        <  2:822349/241‑1 (MQ=255)                                                                                                                                                                                                   acgaGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTTTCATCATCCGTACGCTCGTTTACCTCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGGAATAAATCGAGTTGTTCCTGCGTTGCTTCCGCCAGTCCATTGAAAAACAGGGTaaagaaaag                                                       >  1:603882/1‑247 (MQ=255)                                                                                                                                                                                                     gaGCAAAGAGAAAAATTAGTCCGCTAACCAGCCGTTTTTTTTTTTT‑TTTAAC‑C‑TGGCGT‑TTAA‑T‑TTTTTGTCTC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGcac                                                    <  1:685383/250‑1 (MQ=255)                                                                                                                                                                                                      aGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTGTCTT‑TAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGGTAAAGAAAGGcaca                                                   >  1:887982/1‑250 (MQ=255)                                                                                                                                                                                                         tAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGGCGGACAAAAAATGCTTTAACAATTCAGGGATTAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGGGAAAAAAAGGCACAt                                                  >  1:915628/1‑248 (MQ=255)                                                                                                                                                                                                            gagCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTTTCTT‑CCAACGCCCTGAGAAACTCACCCTGCCCACCGCGCCAGTGCCAACGTTTTTTCATTATCCGTACCCCCGTTTACCGCCGGGCCGGACAAAAAATGCTTTTACAATTCAGGTAATAAATCGAGTTTTTCCTGCGTTGCTGCCGCCCGTCCATTGGAAAACGGGGGAAAGAAAAGCACCAgagg                                              >  2:503640/1‑249 (MQ=255)                                                                                                                                                                                                              gCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTGTCTT‑CAACCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTTAAAAACGGGGTAAAGAAAGGCACATgaggag                                            >  1:356430/1‑249 (MQ=255)                                                                                                                                                                                                                 aaaTTAGTCCGATAATCAGCCTTTATATTTATTG‑TTAAAA‑C‑GGGGGG‑TTGA‑A‑TTGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAAtt                                        <  1:106685/250‑1 (MQ=255)                                                                                                                                                                                                                       tttCGCAAATCAGCCTTTATATTTATTG‑TTAAAA‑C‑GGGCGG‑TTAA‑T‑TTTTTGTCTC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCa                                   <  1:142077/248‑1 (MQ=255)                                                                                                                                                                                                                       gTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTGTCTT‑CAAACGCCCTTAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATTATCATCCGTACGCCCGTTTTCCGCCGGGCTGGGCAAAAACTGCTTTAACAATTCAGGGAATAAAACGAGTTTTTTCTGCGTTGCTGCCGCCAGTCCCTTGAAAAACGGGGTAAAGAAAAGGACATTAGGAGAATTAACCAg                                  >  2:689705/1‑250 (MQ=255)                                                                                                                                                                                                                        ttCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTGTCTT‑CAACCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGGAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAg                                  >  1:633329/1‑249 (MQ=255)                                                                                                                                                                                                                                atctggctttttttttttt‑tttAAAGC‑GGGCGT‑TTAA‑T‑TTGTTGTCTT‑CACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTa                          <  2:565202/238‑1 (MQ=255)                                                                                                                                                                                                                                   catcgtttattttttGTTTTAAA‑C‑CGGGGG‑TTTAGT‑TTTTTCTCTC‑CACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACGATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAAc                        <  2:13107/243‑1 (MQ=255)                                                                                                                                                                                                                                        ttttttttttG‑TTAAAT‑C‑GGGCTG‑TT‑A‑T‑TTTTTTTCTTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGt                  <  2:208071/245‑1 (MQ=255)                                                                                                                                                                                                                                          ttttttttt‑tTAAAA‑C‑TGGCGT‑TTAA‑T‑TTGTTTTCTC‑CACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCg                <  1:660525/247‑1 (MQ=255)                                                                                                                                                                                                                                            ttttttt‑tttAAACC‑GGGCGG‑TTGA‑T‑TTGTTTTCTT‑CACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGc               <  1:494908/249‑1 (MQ=255)                                                                                                                                                                                                                                             ttattG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTTTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGGTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAAAAATTCAGGTAATAAAACGAGTTGTTTCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAAAGCCAGGCGCTgg            >  1:311640/1‑250 (MQ=255)                                                                                                                                                                                                                                             ttattG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTTTCTT‑CAACCGCCATTAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTAACAATTCCGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCCTTGAAAAACGGGGGAAAGAAAAGCACATGAGGAGAATTATCCAGCATATTTAACACCAGTCGCTgg            >  2:80978/1‑250 (MQ=255)                                                                                                                                                                                                                                             ttattG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTTTCTT‑CAACCGCCATGAGAAAATCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCCTCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTTACAATTCAGGGAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGGTAAAAAAAGGCACATGAGGAGAATTATTCAGCATATTTAACGCCAGTCGCTgg            >  2:866734/1‑250 (MQ=255)                                                                                                                                                                                                                                              tattG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTGTCTT‑CAACCGCCATTAGAAAATCACTCTGCCCACAGCGCCAGTGCCCACGGTTTTTCATCATCCGTACGCCCGTTTACCGCCGGGGTGGGCAAAAAATGCTTTAACAATTCAGGGAATAAATCGAGGTGTTCCTGCGTTGCTGCCCCCAGTCCATTGAAAAAAGGGGGGAAAAAAAGCACATGAGGAGAAATATTCAGCATATTTAAAGCCAGTCGCTgg            >  2:358517/1‑249 (MQ=255)                                                                                                                                                                                                                                              tattG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa           >  1:894736/1‑250 (MQ=255)                                                                                                                                                                                                                                                        aa‑C‑TGGCGG‑TTGA‑A‑TTGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCg  >  1:668209/1‑250 (MQ=255)                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                           GTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACC‑TATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTG‑TTTAAA‑C‑TGGCGG‑TTGA‑A‑TTGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCG  >  NZ_CP009273/2144906‑2145400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑