| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,428,361 | 0 | T | G | 39.7% | 152.4 / 13.2 | 78 | Y252S (TAC→TCC) | truA | tRNA pseudouridine(38‑40) synthase TruA |
| Reads supporting (aligned to +/- strand): ref base T (14/33); new base G (31/0); total (45/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGACT > NZ_CP009273/2428115‑2428607 | ttAAGAACAAAATGGCAGAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAAt < 2:581292/250‑1 (MQ=255) tGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGGAACCACCGCGccc > 1:869937/1‑249 (MQ=255) acATAACCCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAAACGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGGAATAGCGGGCCCATTGGCGTTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGa < 2:405267/249‑1 (MQ=255) aaaCCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCCGGGGAATCCACCCCGACCAGAAAc > 1:782542/1‑249 (MQ=255) aaaCCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACCGTCAGGGGAATACACCGCGCCCCCATAc > 2:180880/1‑249 (MQ=255) aaCCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATAACGGTCCGGGGAATCCACCGCCACCAGaaaaaa > 1:185191/1‑245 (MQ=255) aCCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCAAACCCGGCAGGGGAATACACCCCGGCCAGAAACaac > 1:445909/1‑248 (MQ=255) gACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGTCAGGGGAATTCACCGCGACCAGATACAGCCCttt > 1:555495/1‑249 (MQ=255) aCGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTcc < 1:853202/250‑1 (MQ=255) cGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAAATCATACCGGTCAGGGGAATCCACCCCGACCAGATACAGCCCTTCCg > 1:748967/1‑250 (MQ=255) gTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATTCTAACGGGCCGGGGAATCCACCGCGACCACCTACAGCCCCTCCGCctt > 1:182269/1‑250 (MQ=255) gcgACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCCCCGCGACCAGATACAGCCCCTCCGCTTTTTCCGtt > 1:520224/1‑250 (MQ=255) gACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTtgc < 1:531089/250‑1 (MQ=255) ccAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTtgctg < 2:520321/250‑1 (MQ=255) ccAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCg < 2:404415/250‑1 (MQ=255) tGCACGTCAATGTGCAGGATAAAATCGATGAGGAAAT‑AATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTTGGAGATCATACCGGTCAGGGGGAACCACCGCGACCCGAAACAGGCCCTCCCCTTTTGCTGTTGCCGCCGCCCCCGTTCttt > 2:653338/1‑248 (MQ=255) cACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACGGTCAGGGGAATTCACCGCGACCAGATACAGCCCCTCCCCCTTTTCCGTTGCTGCCGCCAGCGTTCTT‑Tc > 1:437986/1‑250 (MQ=255) cACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAACCCTTCCGCCTTTTCCGTTGCTGCCCCCCGCGGTCttgc > 1:11243/1‑247 (MQ=255) gTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGAACATAACGGTCAGGGGAATCCACCCCGACCAGATAAAGCCCTTCCGCTTTTTCCGTTGTTGCCGCCCGCGCTCTG‑ttcct > 2:218610/1‑246 (MQ=255) aaTGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAAACCAACGCGACCAGATACAGCCCTTTCGCTTTTGCCCTTGCTGCCGCCACCGTTCTGTTCCtttc > 1:193302/1‑249 (MQ=255) tgCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTgccgcc < 1:245209/250‑1 (MQ=255) gATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGCTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTTGGAGGTCCAACCCGGCAGGGGAAACCACCCCGCCCCAAAACAACCCCTCCGCCTTTTTCGTTTCTGCCCCCCGCGGTCTT‑TCCTTTtccccccccaa > 2:336142/1‑245 (MQ=255) aTAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGGTTATACCGGTTAGGGTAAACCACCCCGGACCGGTAAAGCACTTCCCCTTTTGTCCGTGTTTCCCCCCGCGGTCCG‑TCCTTTgcccgccaccac > 2:504968/1‑241 (MQ=255) aaaaTCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCAGGGGAATCCACCGCGACCCGATACAACCCTTCCGCCTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCCGCAActc > 1:455893/1‑250 (MQ=255) aaaaTCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAActc < 2:806598/250‑1 (MQ=255) aaTCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTg < 1:345462/250‑1 (MQ=255) aTCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCCGGCCGGGGAATCCACCGCGACCCGATACAGCCCCTCCGCCTTTTCCGGTGCTTCCCCCAGCGTTCTG‑TCCTTTTCCGCCAGCAACTCTGc > 1:209241/1‑250 (MQ=255) tCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGGCAGGGGAATCCCCCCCGCCCAGATACAGCCCCTCCGCTTTTTTCGTTTCTGCCCCCCGCGTTCTG‑TCCTTTTCCCCCCGCCACTCTGCt > 2:674522/1‑250 (MQ=255) gATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTAt < 2:575257/250‑1 (MQ=255) tGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATcc < 1:589072/250‑1 (MQ=255) aGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAg < 1:36375/250‑1 (MQ=255) aaatCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGTAGATCATACCCGTCAGGGGAATCCACCGCGCCCCGAAACAACCCCTCCCCTTTTTCCGTTTCTTCCCCCCGCGTTCTG‑TCCCTTTCCGCCCGCCACTCTCCTCTCCCGCCCCCCg > 2:325032/1‑250 (MQ=255) aGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTTCCGTTGCTGCCGCCCGCGTTCTG‑TCCTTTTCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTgg > 1:281032/1‑250 (MQ=255) gTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGtt < 2:320817/250‑1 (MQ=255) atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACCGGCCGGGGAATCCACCCCGACCCGAAACAACCCCTCCCCCTTTTCCCTTGCCGCCCCCCCCGGTCTT‑TCCCTTTCCCCCCCCCACTCCTCTTTCCCGCCCCCCCGCCGGGTGTgggg > 2:257895/1‑249 (MQ=255) ttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCCGATACAGCCCTTCCGCTTTTTCCGTTGCTGCCCCCAGCGTTCTG‑TCCTTTTCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGGTGTGGGCAc > 1:595890/1‑250 (MQ=255) ttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGAACATAACGGTCAGGGGAATCCACCGCGACCAGATACAGCCCCTCCGCTTTTGCCGTTGCTGCCCCCCGCGGTCTG‑TCCTTTGCCGCCCGCAACTCTGCTATCCAGCTCTCCGGCTGGGTGTGGGCAc > 1:596365/1‑250 (MQ=255) gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACCGGCAGGGGAATCCACCGCGACCAGATAAAGCCCCTCCCCCTTTTCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTTTCGCCCGCAACTCTTCTCTCCAGCTCTCCCGCTGGTTGTGGGCAcc > 1:861667/1‑249 (MQ=255) ccTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCg < 1:75163/249‑1 (MQ=255) tGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCCTCCGCTTTTGCCGTTGCTGCCCCCAGCGTTCTG‑TCCTTTTCCGCCCGCAACTCTGCTATCCCGCCCTCCGGCTGGTTGTGGGGCCCGCCTTc > 1:89364/1‑250 (MQ=255) cTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCCGCGTTCTG‑TCCTTTTCCGCCCGCAACTCTGCTTTCCAGCTCTCCCGCTGGTTGTGGGGACCGACTTCCAt > 1:165683/1‑250 (MQ=255) ttGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGAc > 1:106012/1‑141 (MQ=255) tGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGAcc < 2:106012/141‑1 (MQ=255) gTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc < 1:487856/248‑1 (MQ=255) cTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGGTGTGGGGACCGACTTCCCTCAGGCTGCCGACa > 1:68351/1‑249 (MQ=255) tCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAata < 2:532266/249‑1 (MQ=255) tCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTTGAAGATTATAACCGGCAGGGGAAACCACCCCGACCAGATAAAACCCCTCCGCTTTTTCCGTTGCTGCCGCCCGCGGTTTT‑TTCTTTTTCCCCCGCAACCCTGCTATCCCGCTCTCCGGCTGGGTGTGGGGGCCCACCTTCCTCAAGCTTCCGACAaaat > 2:600592/1‑250 (MQ=255) aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTc < 1:767313/250‑1 (MQ=255) tAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGa < 1:205518/248‑1 (MQ=255) cGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCatat < 2:795747/248‑1 (MQ=255) ttGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATAACGGGCAGGGGAAAACACCGCGACCCGATACAGCCCTTCCCCTTTTTGCGGTGCTGCCGCCCGCGTTCTG‑TCCTTTTCCGCCCGCAACTCTTCTATCCAGCTCCCCCGCTGGGTGTGGGCACCGACTTCCCTCAGGCTGCCGCCAAAATTCTTTACCATatgatg > 2:474349/1‑250 (MQ=255) cAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACaaa < 1:337117/249‑1 (MQ=255) cAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACaa < 2:549060/248‑1 (MQ=255) tGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCtt < 1:227858/250‑1 (MQ=255) gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAACCCCTCCCCTTTTTCCGTTGCTGCCCCCAGCGGTCTG‑TCCTTTTCCGCCCGCAACTCTGCTATCCCGCTCTCCGGCTGGGTGTGGGGACCGACTTCCATCAGGCTGCCGACAAAATTCCTGACCATATGATGTACAAAAAAATTTGCttt > 1:332776/1‑250 (MQ=255) cGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAata < 1:323612/250‑1 (MQ=255) ttCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTaccac < 1:180880/249‑1 (MQ=255) cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACGGGCCGGGGAATCCACCCCGGCCAGAAACAACCCTTCCCCCTTTTCCGGTGCTTCCGCCACCGCTCTG‑TCCTTTGCCGCCCGCCACCCCTCCTTCCCGCTCCCCCGCCGGGTGTGGGGACCGCACTCCCTCAGGCTGCCGACCAAAATTCTTCCCATTTTATGTACCAAAGCACTCGCTTTTATATTCTCCACcaca > 2:827673/1‑249 (MQ=255) gCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATAACGGGCAGGGTAATCCACCCCGACCAGATACAGCCCCTCCGCTTTTTTCGTTGCTGCCCCCCGCGCTCTC‑TCCTTTTCCGCCCGCAACTCTGCTATCCAGCTCTCCCGCTGGGTGTGGGCACCGCCTTCCACCAGGCTGCCGGCAAAAATCCTGTCCATATGATGTACAAAAACAATTGCTTTAATAACTACCACCACAAAAGGaacac > 2:282729/1‑246 (MQ=255) ccATAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGTTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTg < 2:302205/250‑1 (MQ=255) gCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGa < 2:586478/249‑1 (MQ=255) gCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCa > 2:853260/1‑249 (MQ=255) ggTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATaa > 1:379030/1‑250 (MQ=255) gTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAAc > 1:791954/1‑250 (MQ=255) ttttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACg > 2:503478/1‑250 (MQ=255) ttttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACg < 1:393160/250‑1 (MQ=255) tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTg > 1:733267/1‑250 (MQ=255) ggAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTg < 1:542278/249‑1 (MQ=255) ggAAGATCATAACGGTCAGGGTAAACCCACGCGACCGGATACAGCCCTTCAGCTTTAGCCGTTGCTGCCGCCAGCGTTCTG‑GCCTTTGCCGCCAGCAACTCTACTATCCAGCTATCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTAGCGAAAATATTCCTGACCATGTGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTg < 2:551669/249‑1 (MQ=255) gAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTgcg > 2:352713/1‑250 (MQ=255) gATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCa > 1:730567/1‑250 (MQ=255) aTCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCAc < 1:194620/250‑1 (MQ=255) cATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACgg > 1:388983/1‑250 (MQ=255) cGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGc > 1:102616/1‑173 (MQ=255) ggTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCa < 2:102616/173‑1 (MQ=255) aGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGAc < 1:218610/250‑1 (MQ=255) aGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGAc > 1:474209/1‑250 (MQ=255) gggTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGACt > 1:561503/1‑250 (MQ=255) | TTAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTG‑TCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGACT > NZ_CP009273/2428115‑2428607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |