| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 681,493 | 0 | C | A | 45.5% | 6.5 / 10.7 | 22 | S211S (TCG→TCT) | gltJ | glutamate/aspartate ABC transporter permease GltJ |
| Reads supporting (aligned to +/- strand): ref base C (6/6); new base A (10/0); total (16/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATAAACGCATCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGG‑ACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTG‑AGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGG > NZ_CP009273/681247‑681695 | aTAAACGCATCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGG‑ACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGGAAAAGa > 1:299093/1‑249 (MQ=255) ccAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGG‑ACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGTAAAACAATCCCAAGccaggggc > 1:911639/1‑243 (MQ=255) cAGCATCGCCCCCCCAAAAATCCCAACCCCACCCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGG‑ACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTg < 2:672258/250‑1 (MQ=255) tcccAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGG‑ACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTG‑AGTAATCGAGCAATTTAccc < 1:147777/248‑1 (MQ=255) cAATCCCTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGG‑ACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTG‑AGTAATCGAGCAATTTACCCGcc < 1:517876/250‑1 (MQ=255) aaTCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGG‑ACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGGAAAAGACTCCCCCGCAAGGGGGG‑GGGAAACGAGCAAATTAACccccc > 1:314324/1‑249 (MQ=255) tACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGG‑ACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGGAAAAAACTCCCAGGCACGGGCTG‑AGGAAACGAGGAAATTACCCCCCCGcgccc > 1:299785/1‑249 (MQ=255) gccgTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGG‑ACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGGAAAAGACCCCCAGGCCAGGGGTG‑GGGAATCGAGCAAATTTCCCCCCTGCgcccccc > 1:809846/1‑248 (MQ=255) ccgTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGG‑ACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGTAAAAGACTCCCCCGCAAGGGCTG‑AGTAATCGAGCAATTTTCCCCCCTGCGCCGCCata > 1:784208/1‑250 (MQ=255) gCAGATA‑GGCAGGGGAGGGAACAAGGAAACCCCAATAAAACTATTAC‑TTTTTTCCCCCCCCTGTTCCCCGGGGGGCCAAATTTTCCTTCCAACCACCGTCACCCCCAGAAAAAAAAAAGC‑TTAACCAACCCCATCCCCCGGC‑AAAGGGGCGAAAACCGCCCCCCGGGAGGGGGTGAATTAACCGAAAATTTTCCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTc < 2:67153/250‑1 (MQ=255) aGATATGGCAGGGAAGGG‑ACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGGAAAAGACTCCCAGGGATGGGGTG‑AGGAATAGAGGAATTTAACCGCCTGCGCCGCCATAACCACCAGACCGAAAGGCGAGGGGGTGGCGGGGgtttttaa > 1:60725/1‑247 (MQ=255) ggCAGGGAAGGG‑ACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTG‑CGTCATCGAGCAAATTAACCGCCTGCGCCGCCCTATCCACCAGACCCCTAGTCGCGGCGATGGCGGAGATTTTTACCAgggt > 1:673898/1‑248 (MQ=255) cAGGGAAGGG‑ACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGTAAAAGACTCCCAGGCATTGGGTG‑AGTAATCGAGCAATTTACCCGCCCGCGCCGCCATATCCACCCGACCGGTAGTTGAGGCGAAGGCGGGGGTTTTCAcccaggtca > 1:238393/1‑243 (MQ=255) aaTGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGTAAAAGACTCCCCGGCATGGGCTG‑AGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCCCCAGACCGATAGTCGAGGCGTTGGCGGGGTTTTTTCCCAGGTTCATCATCTCTGAgg > 1:655580/1‑250 (MQ=255) aTGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTACCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTG‑AGGAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCCGGTTCATCATCTCTGAGGt > 1:526828/1‑250 (MQ=255) cAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTG‑AGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGGTGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggcgg > 1:916308/1‑250 (MQ=255) tACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGTAAAAGACTCCCAAGCATGGGCTG‑GGGAATCGAGCAATTTACCCCCCTGCGCCGCCCTATCCACCCGACCGATAATCGAGGCGGTGGCGGGGGTTTTTACCAGGGTCCTCATCCCTGAGGTCATCGGCggggggagaaaac > 2:2798/1‑239 (MQ=255) aCATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTG‑AGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATTCACCAGACCGATAGTCGAGGCGATGGCGGAGGTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCgggggggctaacgga > 1:554887/1‑238 (MQ=255) ttattTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTG‑AGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAAACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGGCGCTAAagcgcga > 1:83373/1‑246 (MQ=255) ttattTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGTAAACCACTCCCAGGCATGGGGTG‑AGTAATCGAGCAAATTACCCGCCCGCGCCGCCCTAACCACCAGACCGAAAGACGAGGCGATGGCGGAGGTTTTTACCACGGTCATCATCTCTTGGGTCATCGGCGGGGCgggaaagagcta > 2:429764/1‑238 (MQ=255) cagctacacccccAGCATAAAAAAGGCTTAAATCAA‑CACACCCGCCAGCGAAAGGGGGATAACCGCCCCCCGGGCGGGGGCTG‑AAAAACGGAGCATTTTCCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAgg < 1:606321/239‑1 (MQ=255) ataaaaaaaGCTTTAAAAAA‑CACACCCCCGAGTGAAAGGGGGGAAACCCACCCCCGGGCGGGGGCTA‑AAAAACCGAGAATTTCCCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCgg < 1:889758/245‑1 (MQ=255) | ATAAACGCATCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGG‑ACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTT‑CAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAA‑CACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTG‑AGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGG > NZ_CP009273/681247‑681695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |