Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435232,059,8030AT61.7% 889.2 / inf 942T386T (ACT→ACA) FTN78_chr2133D‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base A (135/225);  new base T (296/284);  total (431/511)
Fisher's exact test for biased strand distribution p-value = 5.32e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 962 total aligned reads displayed.

TAGAGTTTTTCATTAACAAAATTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAAC  >  CP043523/2059654‑2059952
                                                                                                                                                     |                                                                                                                                                     
tAGAGTTTTTCATTAACAAAATTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAaga                                                                                                                                                       >  2:1626183/1‑150 (MQ=255)
   agTTTTTCATTAACAAAATTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTg                                                                                                                                                    <  1:1521815/150‑1 (MQ=255)
    gTTTTTCATTAACGAAATTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGa                                                                                                                                                   >  1:2481260/1‑150 (MQ=255)
     tttttCATTAACGAAATTTACAAAATGATTCCAAATAACCTTTTGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGAc                                                                                                                                                  <  2:2954864/150‑1 (MQ=255)
     tttatCATTAACAAAATTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGAc                                                                                                                                                  <  1:4106852/150‑1 (MQ=255)
      ttttCATTAACGAAATTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACa                                                                                                                                                 <  1:1349633/150‑1 (MQ=255)
       tttCATTAACGAAATTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGTGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACaa                                                                                                                                                <  2:2862207/150‑1 (MQ=255)
        ttCATTAACGAAATTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAg                                                                                                                                               >  2:225440/1‑150 (MQ=255)
         tCATTAACAAAATTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGt                                                                                                                                              >  1:645351/1‑150 (MQ=255)
          cATTAACGAAATTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGtt                                                                                                                                             >  1:1946532/1‑150 (MQ=255)
          cATTAACAAAATTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGtn                                                                                                                                             <  2:3789116/150‑2 (MQ=255)
            ttAACGAAATTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGtttt                                                                                                                                           <  1:1425195/150‑1 (MQ=255)
             tAACAAAATTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGttttg                                                                                                                                          <  2:1580789/150‑2 (MQ=255)
                  aaaTTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGttt                                                                                                                                     >  1:189056/1‑150 (MQ=255)
                  aaaTTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGttt                                                                                                                                     >  1:3180013/1‑150 (MQ=255)
                   aaTTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGtttt                                                                                                                                    >  1:491692/1‑150 (MQ=255)
                   aaTTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGtttt                                                                                                                                    <  1:2100883/150‑1 (MQ=255)
                    aTTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGttttt                                                                                                                                   <  2:3037642/150‑1 (MQ=255)
                      ttACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAg                                                                                                                                 >  1:2969791/1‑150 (MQ=255)
                      ttACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAg                                                                                                                                 >  1:2679204/1‑150 (MQ=255)
                        aCAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTc                                                                                                                               >  1:4226683/1‑150 (MQ=255)
                        aCAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTCACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTc                                                                                                                               >  1:4496257/1‑150 (MQ=255)
                         cAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTca                                                                                                                              <  1:2312326/150‑1 (MQ=255)
                             aTGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGt                                                                                                                          >  2:2779975/1‑150 (MQ=255)
                               gATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTg                                                                                                                        >  2:3370051/1‑150 (MQ=255)
                                aTTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGc                                                                                                                       >  2:17429/1‑150 (MQ=255)
                                aTTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGc                                                                                                                       <  2:392351/150‑1 (MQ=255)
                                  tCCAAAAAACCATTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACGAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCtt                                                                                                                     >  2:4071222/1‑150 (MQ=255)
                                   ccAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTc                                                                                                                    <  1:1171610/150‑1 (MQ=255)
                                    cAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCa                                                                                                                   <  1:2158209/150‑1 (MQ=255)
                                     aaaaaaCCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCaa                                                                                                                  <  2:3284880/150‑1 (MQ=255)
                                       aaaaCCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAAct                                                                                                                <  1:283985/150‑2 (MQ=255)
                                       aaaaCCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACg                                                                                                                >  1:2593297/1‑150 (MQ=255)
                                        aaaCCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAActg                                                                                                               >  2:4343424/1‑148 (MQ=255)
                                          aCCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAg                                                                                                             >  2:1244549/1‑150 (MQ=255)
                                          aCCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGCAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAg                                                                                                             >  1:4028166/1‑150 (MQ=255)
                                            cTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGc                                                                                                           <  1:1267703/150‑1 (MQ=255)
                                             tttGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCTTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCt                                                                                                          <  2:4554446/150‑1 (MQ=255)
                                                ggAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGtt                                                                                                       >  2:3432210/1‑150 (MQ=255)
                                                ggAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGtt                                                                                                       >  1:845660/1‑150 (MQ=255)
                                                ggAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGtt                                                                                                       <  2:3130525/150‑1 (MQ=255)
                                                ggAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGtt                                                                                                       >  1:2240882/1‑150 (MQ=255)
                                                    cgcTACTACGGGGGACAGTTTTCTTAGCATCCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGtttttt                                                                                                   <  1:3534272/150‑1 (MQ=255)
                                                    cgcTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGtttttt                                                                                                   <  1:3891023/150‑1 (MQ=255)
                                                    cgcTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGtttttt                                                                                                   >  1:3835334/1‑150 (MQ=255)
                                                    cgcTACTACGGGGGACAGTTTTCCTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGtttttt                                                                                                   <  2:1336676/150‑1 (MQ=255)
                                                      ctactaCGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGtttttttc                                                                                                 <  1:3734264/150‑3 (MQ=255)
                                                       tactacGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGtttttttct                                                                                                >  1:2027382/1‑147 (MQ=255)
                                                       tactacGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCt                                                                                                <  2:879735/150‑1 (MQ=255)
                                                        actacGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAACCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCtt                                                                                               <  1:3978277/150‑1 (MQ=255)
                                                         ctacGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTa                                                                                              <  1:94704/150‑1 (MQ=255)
                                                          tacGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTAc                                                                                             >  1:574956/1‑150 (MQ=255)
                                                          tacGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTAc                                                                                             <  2:2364651/150‑1 (MQ=255)
                                                             gggggACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGa                                                                                          <  1:1000692/150‑1 (MQ=255)
                                                                  aCAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTg                                                                                     >  1:3098114/1‑150 (MQ=255)
                                                                   cAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGa                                                                                    >  1:3289140/1‑150 (MQ=255)
                                                                              gCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGan                                                                         <  1:520149/150‑2 (MQ=255)
                                                                                aaCCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGAttt                                                                       >  2:612819/1‑150 (MQ=255)
                                                                                 aCCAATGGAATTTGTTTTGCGGTACAATTAGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGAtttt                                                                      <  2:476290/150‑1 (MQ=255)
                                                                                    aaTGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTa                                                                   >  2:4143767/1‑150 (MQ=255)
                                                                                    aaTGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTa                                                                   <  2:518182/150‑1 (MQ=255)
                                                                                     aTGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTAt                                                                  >  1:728932/1‑150 (MQ=255)
                                                                                     aTGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTAt                                                                  <  1:4579845/150‑1 (MQ=255)
                                                                                     aTGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTCTTCCTTACTGAATTTGACTGTCAGTGATTTTGTan                                                                  <  2:4286311/150‑2 (MQ=255)
                                                                                      tGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATc                                                                 <  2:4202464/150‑1 (MQ=255)
                                                                                        gAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTCTCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTg                                                               <  1:4349126/150‑1 (MQ=255)
                                                                                         aaTTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGc                                                              >  1:4409625/1‑150 (MQ=255)
                                                                                             tGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTa                                                          >  2:3640593/1‑150 (MQ=255)
                                                                                                  tGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATc                                                     >  1:2539360/1‑150 (MQ=255)
                                                                                                     ggTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGt                                                  <  1:1059036/150‑1 (MQ=255)
                                                                                                     ggTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGt                                                  >  1:3608397/1‑150 (MQ=255)
                                                                                                     ggTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGt                                                  <  2:1902753/150‑1 (MQ=255)
                                                                                                      gTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTa                                                 <  1:3063405/150‑1 (MQ=255)
                                                                                                          aaTTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTGAGTGATTTTGTATCTGCACTACCATCTGTAGcat                                             >  2:4484173/1‑148 (MQ=255)
                                                                                                          aaTTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGt                                             >  1:3458267/1‑150 (MQ=255)
                                                                                                           ntttGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCCTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGcata                                            >  2:20831/2‑147 (MQ=255)
                                                                                                                gAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGaa                                       <  1:1744355/150‑1 (MQ=255)
                                                                                                                 aaGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGaac                                      >  1:3220377/1‑150 (MQ=255)
                                                                                                                    aTAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGaacaac                                   >  2:1504457/1‑150 (MQ=255)
                                                                                                                      aGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACgg                                 >  1:2790640/1‑150 (MQ=255)
                                                                                                                       gCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTATTTAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACgag                                >  2:1129340/1‑148 (MQ=255)
                                                                                                                         cTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAg                              >  1:692562/1‑150 (MQ=255)
                                                                                                                          tAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGt                             <  2:104276/150‑1 (MQ=255)
                                                                                                                           aaaCTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTATTTTTTATTCACAGTTGCTTCAACTGAGGCTATTTTTTTCTTTCTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTg                            >  2:4043497/1‑150 (MQ=255)
                                                                                                                            aaCTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTTAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGa                           >  2:398445/1‑150 (MQ=255)
                                                                                                                            aaCTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTGGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGGTTTTGTATCTGCACTGCCATCTGTAGCATCAGGAACAACGGGAGTGa                           >  1:2349496/1‑150 (MQ=255)
                                                                                                                            aaCTATCCTTTACTGTAGTAGAAAGAGTGACAAGATTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGa                           >  1:2905861/1‑150 (MQ=255)
                                                                                                                                 tCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATAttt                      <  2:1983623/150‑1 (MQ=255)
                                                                                                                                 tCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATAttt                      >  2:2160981/1‑150 (MQ=255)
                                                                                                                                 tCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATAttt                      <  1:3364039/150‑1 (MQ=255)
                                                                                                                                  ccTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATAtttt                     >  2:740797/1‑150 (MQ=255)
                                                                                                                                       aCTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGa                >  2:2327957/1‑150 (MQ=255)
                                                                                                                                       aCTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGa                <  2:2678922/150‑1 (MQ=255)
                                                                                                                                         tGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGc              <  2:2561879/150‑1 (MQ=255)
                                                                                                                                              tagAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTGGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGa         >  2:789940/1‑150 (MQ=255)
                                                                                                                                                gAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGt       <  2:3488645/150‑1 (MQ=255)
                                                                                                                                                 aaaGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGtt      <  1:3422582/150‑1 (MQ=255)
                                                                                                                                                 aaaGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGtt      <  2:2481260/150‑1 (MQ=255)
                                                                                                                                                 aaaGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGtt      <  1:1623797/150‑1 (MQ=255)
                                                                                                                                                     agTGACAAGTTTTTTTTTTTTTTTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAAc  >  2:1396344/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAGAGTTTTTCATTAACAAAATTTACAAAATGATTCCAAAAAACCTTTGGAGCGCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAAC  >  CP043523/2059654‑2059952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑