Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435232,059,8560CT61.7% 676.7 / inf 938E369K (GAA→AAA) FTN78_chr2133D‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base C (166/193);  new base T (279/300);  total (445/493)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 946 total aligned reads displayed.

GCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATT  >  CP043523/2059707‑2060005
                                                                                                                                                     |                                                                                                                                                     
gcTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTc                                                                                                                                                       >  1:3444847/1‑150 (MQ=255)
    ctacGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAATATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTa                                                                                                                                                   <  2:1132806/150‑1 (MQ=255)
    ctacGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTa                                                                                                                                                   <  2:657081/150‑1 (MQ=255)
       cGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTg                                                                                                                                                <  2:3941233/150‑1 (MQ=255)
        gggggACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGGTTTTTTCTTACTgc                                                                                                                                               >  2:2866425/1‑149 (MQ=255)
        gggggACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGa                                                                                                                                               <  1:1000692/150‑1 (MQ=255)
         ggggACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGaa                                                                                                                                              <  1:321835/150‑1 (MQ=255)
         ggggACAGTCTTCTTATCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGaa                                                                                                                                              <  1:3681478/150‑1 (MQ=255)
          gggACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAAt                                                                                                                                             <  2:209444/150‑1 (MQ=255)
           ggACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAAtt                                                                                                                                            >  1:2564129/1‑150 (MQ=255)
           ggACAGTTTACTTAGCAACCAATGCAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAAtt                                                                                                                                            >  2:3832019/1‑150 (MQ=255)
             aCAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTg                                                                                                                                          >  2:513809/1‑150 (MQ=255)
              cAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGa                                                                                                                                         >  2:1325429/1‑150 (MQ=255)
               aGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGAc                                                                                                                                        >  2:2020562/1‑150 (MQ=255)
                    tCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCa                                                                                                                                   <  2:2953220/150‑1 (MQ=255)
                       tAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTg                                                                                                                                >  1:582545/1‑150 (MQ=255)
                        aGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGGGACGAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGa                                                                                                                               >  2:4192754/1‑150 (MQ=255)
                         gCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGAt                                                                                                                              >  1:1904522/1‑150 (MQ=255)
                         gCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGAt                                                                                                                              <  2:4314515/150‑1 (MQ=255)
                           aaCCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGAttt                                                                                                                            >  1:2793503/1‑150 (MQ=255)
                            aCCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGAtttt                                                                                                                           <  2:4244327/150‑1 (MQ=255)
                               aaTGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTa                                                                                                                        >  2:56654/1‑150 (MQ=255)
                                aTGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTAt                                                                                                                       >  1:728932/1‑150 (MQ=255)
                                  ggAATTTGTTCTGCGGTACAATTTGGAGGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCt                                                                                                                     <  1:450071/150‑1 (MQ=255)
                                  ggAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCt                                                                                                                     >  1:1254702/1‑150 (MQ=255)
                                    aaTTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGc                                                                                                                   <  1:3558770/150‑1 (MQ=255)
                                    aaTTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGc                                                                                                                   >  2:2729176/1‑150 (MQ=255)
                                    aaTTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGc                                                                                                                   <  1:429540/150‑1 (MQ=255)
                                      tttGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCAc                                                                                                                 <  2:1073450/150‑1 (MQ=255)
                                        tGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTa                                                                                                               <  1:4378301/150‑1 (MQ=255)
                                         gTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTAc                                                                                                              <  2:2404195/150‑1 (MQ=255)
                                            cTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCAt                                                                                                           >  2:473612/1‑150 (MQ=255)
                                             tGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATc                                                                                                          >  1:2539360/1‑150 (MQ=255)
                                                ggTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGt                                                                                                       <  2:1902753/150‑1 (MQ=255)
                                                  tACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTATTTTCTTTTTCTTCAACTGAGGCTGTTTTTTTTTTACTTAATTTTACTGTCAGTGATTTTGTTTTTTCTCTACCATCTGTAg                                                                                                     >  2:832002/1‑150 (MQ=255)
                                                  tACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAg                                                                                                     <  2:893991/150‑1 (MQ=255)
                                                     aaTTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGGTTTTGGTCTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGcat                                                                                                  >  1:3993435/1‑148 (MQ=255)
                                                      aTTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTa                                                                                                 <  1:2343105/150‑1 (MQ=255)
                                                       tttGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTaa                                                                                                <  1:2483819/150‑1 (MQ=255)
                                                        ttGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAg                                                                                               >  1:210981/1‑150 (MQ=255)
                                                              gATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGaacaa                                                                                         <  2:1946532/150‑1 (MQ=255)
                                                               aTAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGaacaac                                                                                        <  1:40620/150‑1 (MQ=255)
                                                                 aGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACgg                                                                                      >  2:3108679/1‑150 (MQ=255)
                                                                 aGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACgg                                                                                      <  2:770106/150‑1 (MQ=255)
                                                                 aGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACgg                                                                                      >  1:1318604/1‑150 (MQ=255)
                                                                    cTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAg                                                                                   >  1:692562/1‑150 (MQ=255)
                                                                    cTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAg                                                                                   >  2:3994804/1‑150 (MQ=255)
                                                                       aaCTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGa                                                                                >  1:2888641/1‑150 (MQ=255)
                                                                        aCTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGat                                                                               >  2:1790247/1‑150 (MQ=255)
                                                                        aCTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGat                                                                               <  2:405378/150‑1 (MQ=255)
                                                                             ccTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATAtttt                                                                          <  1:3179496/150‑1 (MQ=255)
                                                                                ttACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTtgtg                                                                       >  1:543253/1‑150 (MQ=255)
                                                                                  aCTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGa                                                                     >  2:2327957/1‑150 (MQ=255)
                                                                                  aCTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCAGCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGa                                                                     <  2:4496257/150‑1 (MQ=255)
                                                                                       agtagAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAAc                                                                >  1:4601617/1‑150 (MQ=255)
                                                                                           gAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGt                                                            >  1:3922501/1‑150 (MQ=255)
                                                                                            aaaGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGtt                                                           <  1:1626183/150‑1 (MQ=255)
                                                                                             aaGAGTGACAAGTTTTTTTTTTTTTTTCACAGTTGCTACAACGGAGGCTGTTTTTTCCTTTCTGAATTTGTCTGTCAGTGATTTTTTATCTGCACTACCATCTGTAGTGTAAGGTATATTGGGAGTGATATTTTGTGGAGCAACGAGTTg                                                          >  2:3444978/1‑150 (MQ=255)
                                                                                               gagTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGaa                                                        <  2:2183489/150‑1 (MQ=255)
                                                                                                agTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAAc                                                       >  2:1372816/1‑150 (MQ=255)
                                                                                                 gTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACa                                                      <  2:3039841/150‑1 (MQ=255)
                                                                                                    aCAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGAt                                                   >  1:3084341/1‑150 (MQ=255)
                                                                                                     cAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGAtt                                                  >  1:2956012/1‑150 (MQ=255)
                                                                                                        gTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGAttttt                                               >  1:328327/1‑150 (MQ=255)
                                                                                                         tttttGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATttttc                                              >  1:2256054/1‑150 (MQ=255)
                                                                                                         tttttGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATttttc                                              >  1:3852862/1‑150 (MQ=255)
                                                                                                           tttGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATttttctt                                            >  2:2521582/1‑150 (MQ=255)
                                                                                                            ttGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATttttcttt                                           <  2:2245346/150‑1 (MQ=255)
                                                                                                             tGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATttttctttt                                          >  1:1161127/1‑150 (MQ=255)
                                                                                                               gTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTcc                                        >  1:651090/1‑150 (MQ=255)
                                                                                                                tttttAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTTCTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCa                                       >  2:3658516/1‑150 (MQ=255)
                                                                                                                 ttttAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCat                                      <  1:2178238/150‑1 (MQ=255)
                                                                                                                   ttAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCatca                                    <  2:1459152/150‑1 (MQ=255)
                                                                                                                     aGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCatcagt                                  >  1:3408726/1‑148 (MQ=255)
                                                                                                                     aGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCatcaat                                  >  1:909534/1‑150 (MQ=255)
                                                                                                                      gTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCatcagtc                                 >  1:146812/1‑150 (MQ=255)
                                                                                                                      gTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGCTATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCatcaatc                                 >  2:1003302/1‑150 (MQ=255)
                                                                                                                        cacaaaagCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGAGATTTTATATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTcac                               >  2:3373110/8‑150 (MQ=255)
                                                                                                                        cacaGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTcac                               <  2:2564129/150‑1 (MQ=255)
                                                                                                                          caGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAg                             <  2:2807227/150‑1 (MQ=255)
                                                                                                                           aGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAgc                            <  2:1250044/150‑2 (MQ=255)
                                                                                                                           aGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAgc                            <  1:3810481/150‑2 (MQ=255)
                                                                                                                            gTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGAGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAgca                           >  2:2648215/1‑148 (MQ=255)
                                                                                                                            gTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTa                           >  1:2649595/1‑150 (MQ=255)
                                                                                                                              tGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAAt                         >  2:589017/1‑150 (MQ=255)
                                                                                                                               gCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAAtt                        >  2:1598206/1‑150 (MQ=255)
                                                                                                                                cTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAAttt                       <  1:4143767/150‑1 (MQ=255)
                                                                                                                                  tCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCt                     >  1:1563600/1‑150 (MQ=255)
                                                                                                                                   cAACGGAGGCTGTTTTTTTTTTTTTTAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCtt                    >  2:3220754/1‑150 (MQ=255)
                                                                                                                                     actgAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTa                  >  1:1082830/4‑150 (MQ=255)
                                                                                                                                      ctgAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTaa                 >  1:3019809/3‑150 (MQ=255)
                                                                                                                                      cGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTaa                 <  2:1652720/150‑1 (MQ=255)
                                                                                                                                       tgtggCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTaaa                <  1:490527/147‑1 (MQ=255)
                                                                                                                                         aGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAat              >  1:1989516/1‑150 (MQ=255)
                                                                                                                                         aGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAat              >  1:2845506/1‑150 (MQ=255)
                                                                                                                                             tGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAatcatc          <  2:3509481/150‑1 (MQ=255)
                                                                                                                                             tGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAatcatc          >  2:4110409/1‑150 (MQ=255)
                                                                                                                                               ttttttTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTa        <  1:3341322/150‑1 (MQ=255)
                                                                                                                                               ttttttTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTa        <  1:1719824/150‑1 (MQ=255)
                                                                                                                                               ttttttCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTa        <  2:4401585/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTACTACGGGGGACAGTTTTCTTAGCAACCAATGGAATTTGTTCTGCGGTACAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATT  >  CP043523/2059707‑2060005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑