Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435232,059,9080GA61.9% 647.0 / inf 954Y351Y (TAC→TAT) FTN78_chr2133D‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base G (222/141);  new base A (298/292);  total (521/433)
Fisher's exact test for biased strand distribution p-value = 1.62e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-02
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 959 total aligned reads displayed.

CAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATAAACATAGTTCAT  >  CP043523/2059759‑2060057
                                                                                                                                                     |                                                                                                                                                     
     tGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAgg                                                                                                                                                  >  2:458040/1‑150 (MQ=255)
        aaGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGaac                                                                                                                                               >  1:1861853/1‑150 (MQ=255)
         aGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGaaca                                                                                                                                              >  2:26644/1‑150 (MQ=255)
           aTAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGaacaac                                                                                                                                            <  1:17429/150‑1 (MQ=255)
             aGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACgg                                                                                                                                          >  2:3108679/1‑150 (MQ=255)
               ccTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGa                                                                                                                                        <  1:4018107/150‑1 (MQ=255)
                  aaaCTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTg                                                                                                                                     >  2:1527340/1‑150 (MQ=255)
                  aaaCTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTg                                                                                                                                     <  2:543202/150‑1 (MQ=255)
                    aCTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGat                                                                                                                                   >  1:2869387/1‑150 (MQ=255)
                      tATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAAAGGAGGCTGTTTTTTCCTTACTGCATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGatat                                                                                                                                 <  2:1915222/150‑1 (MQ=255)
                        tCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATAttt                                                                                                                               >  2:2160981/1‑150 (MQ=255)
                         ccTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATAtttt                                                                                                                              <  1:3184694/150‑1 (MQ=255)
                         ccTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATAtttt                                                                                                                              >  1:697439/1‑150 (MQ=255)
                            ttACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTtgtg                                                                                                                           >  1:543253/1‑150 (MQ=255)
                            ttACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTtgtg                                                                                                                           <  2:647690/150‑1 (MQ=255)
                             tACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTgg                                                                                                                          <  2:4185122/150‑1 (MQ=255)
                               cTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAg                                                                                                                        >  1:2405690/1‑150 (MQ=255)
                                tGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGc                                                                                                                       >  1:2545829/1‑150 (MQ=255)
                                   agtagAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAAc                                                                                                                    >  1:2688165/1‑150 (MQ=255)
                                     tagAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGa                                                                                                                  <  2:1098317/150‑1 (MQ=255)
                                      agAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAg                                                                                                                 <  1:44974/150‑1 (MQ=255)
                                      agAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAg                                                                                                                 >  1:2993811/1‑150 (MQ=255)
                                       gAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGt                                                                                                                <  2:750051/150‑1 (MQ=255)
                                       gAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGt                                                                                                                >  1:3922501/1‑150 (MQ=255)
                                         aaGAGTGACAAGTTTTTTTTTTTTTTTCACAGTTGCTACAACGGAGGCTGTTTTTTCCTTTCTGAATTTGTCTGTCAGTGATTTTTTATCTGCACTACCATCTGTAGTGTAAGGTATATTGGGAGTGATATTTTGTGGAGCAACGAGTTg                                                                                                              >  2:3444978/1‑150 (MQ=255)
                                            tgTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAAc                                                                                                           >  2:493765/2‑150 (MQ=255)
                                            agTGACAAGTTTTTTGTTTTTTTTTACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTATTGTACTACGGGATTGATATTTTGTGGAGCAACGAGTTGAAc                                                                                                           >  2:3214338/1‑150 (MQ=255)
                                             gTGACAAGTTTTTTGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAAcn                                                                                                          <  1:2638114/150‑2 (MQ=255)
                                              tGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAg                                                                                                         >  1:1670096/1‑150 (MQ=255)
                                               gACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGa                                                                                                        >  1:195710/1‑150 (MQ=255)
                                                   aGTTTTTGGTTTTTTTTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGAtttt                                                                                                    >  2:4357870/1‑150 (MQ=255)
                                                   aGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGAgttt                                                                                                    >  1:4088545/1‑150 (MQ=255)
                                                    gTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGAttttt                                                                                                   <  2:2348099/150‑1 (MQ=255)
                                                     tttttGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATttttc                                                                                                  <  2:4028166/150‑1 (MQ=255)
                                                       tttGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATttttctt                                                                                                >  2:2521582/1‑150 (MQ=255)
                                                        ttGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATttttcttt                                                                                               <  2:2245346/150‑1 (MQ=255)
                                                         tGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATttttctttt                                                                                              <  1:484531/150‑1 (MQ=255)
                                                              tttAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCatc                                                                                         <  2:911276/150‑1 (MQ=255)
                                                                 aGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGTAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCatcagn                                                                                      <  2:134014/150‑3 (MQ=255)
                                                                 aGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCatcagt                                                                                      <  2:127325/150‑3 (MQ=255)
                                                                 aGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCatcagt                                                                                      >  1:3408726/1‑148 (MQ=255)
                                                                 aGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCatcaat                                                                                      >  1:909534/1‑150 (MQ=255)
                                                                  gTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCatcagtc                                                                                     >  1:147176/1‑147 (MQ=255)
                                                                   tCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCatcaatca                                                                                    <  1:308690/150‑1 (MQ=255)
                                                                    cacaaaagCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGAGATTTTATATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTcac                                                                                   >  2:3373110/8‑150 (MQ=255)
                                                                     acaGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTcaca                                                                                  <  2:1670096/150‑1 (MQ=255)
                                                                      caGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAg                                                                                 <  2:3054484/150‑1 (MQ=255)
                                                                      caGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAg                                                                                 >  1:1250051/1‑150 (MQ=255)
                                                                      caGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAg                                                                                 <  2:2807188/150‑1 (MQ=255)
                                                                       aGTTGCTTCAACTGAGGATGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAgc                                                                                <  1:4170987/150‑2 (MQ=255)
                                                                           gCTTCAACGGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAAtt                                                                            >  2:4470703/1‑150 (MQ=255)
                                                                            cTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAAttt                                                                           <  1:3836857/150‑1 (MQ=255)
                                                                              tCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCt                                                                         <  1:3758517/150‑1 (MQ=255)
                                                                                   tgAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTaaa                                                                    >  1:3642766/2‑150 (MQ=255)
                                                                                    gAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTaaaa                                                                   <  1:4309324/150‑1 (MQ=255)
                                                                                      ggCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAatc                                                                 <  1:2824561/150‑1 (MQ=255)
                                                                                        cTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAatcat                                                               <  2:2863223/150‑1 (MQ=255)
                                                                                         tGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATATGCACTACCATCTGTAGCATAATGAACATCGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAatcatc                                                              <  2:3509766/150‑1 (MQ=255)
                                                                                           ttttttTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTa                                                            <  1:3341322/150‑1 (MQ=255)
                                                                                             ttttTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAg                                                          >  1:277907/1‑150 (MQ=255)
                                                                                               tttcTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGga                                                        >  1:2271963/4‑150 (MQ=255)
                                                                                                  cTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGgattt                                                     <  2:3289140/150‑2 (MQ=255)
                                                                                                  cTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGgattt                                                     <  2:196691/150‑2 (MQ=255)
                                                                                                  cTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGgattt                                                     >  1:1574206/1‑149 (MQ=255)
                                                                                                    tACTGAATTTGACTGTCAGTGATATTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGCTATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAATATCATCTAAGgatttgt                                                   >  2:298136/1‑147 (MQ=255)
                                                                                                        gAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGAttttt                                               >  1:2505070/1‑150 (MQ=255)
                                                                                                        gAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTAttttt                                               <  2:2027382/150‑1 (MQ=255)
                                                                                                        gAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTAttttt                                               <  2:1012929/150‑1 (MQ=255)
                                                                                                         aaTTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGAtttttt                                              <  2:592615/150‑1 (MQ=255)
                                                                                                          aTTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTAttttttt                                             <  1:20769/150‑1 (MQ=255)
                                                                                                             tGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGca                                          >  1:3258596/1‑150 (MQ=255)
                                                                                                             tGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGca                                          >  1:2309487/1‑150 (MQ=255)
                                                                                                               aCTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGcaca                                        >  1:4401560/1‑150 (MQ=255)
                                                                                                               aCTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGcaca                                        >  1:3489132/1‑150 (MQ=255)
                                                                                                                 tGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAg                                      <  1:110905/150‑1 (MQ=255)
                                                                                                                  gTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGa                                     >  2:4266459/1‑150 (MQ=255)
                                                                                                                   tCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGAc                                    >  1:1187210/1‑150 (MQ=255)
                                                                                                                      gTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTgg                                 <  2:3761890/150‑1 (MQ=255)
                                                                                                                       tGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGt                                <  2:210981/150‑1 (MQ=255)
                                                                                                                        gATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTc                               >  1:2938441/1‑150 (MQ=255)
                                                                                                                          ttttGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGt                             <  2:794829/150‑1 (MQ=255)
                                                                                                                           tttGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTa                            <  2:2607496/150‑1 (MQ=255)
                                                                                                                           tttGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGTCGTa                            >  2:4079512/1‑150 (MQ=255)
                                                                                                                           nttGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTa                            >  2:1604087/2‑150 (MQ=255)
                                                                                                                            ttGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTCAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTAc                           <  2:3888726/150‑1 (MQ=255)
                                                                                                                            ttGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGTCGTAc                           <  1:1618600/150‑1 (MQ=255)
                                                                                                                              gTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGTCGTACGc                         >  1:632817/1‑150 (MQ=255)
                                                                                                                              gTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGTCGTACGc                         >  1:4583714/1‑150 (MQ=255)
                                                                                                                                  cTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGTCGTACGCCAAt                     <  1:816859/150‑1 (MQ=255)
                                                                                                                                   tGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATg                    >  2:1390203/1‑150 (MQ=255)
                                                                                                                                      aCTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGTCGTACGCCAATGAcc                 >  1:1332953/1‑150 (MQ=255)
                                                                                                                                        tACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCAt               >  2:398161/1‑150 (MQ=255)
                                                                                                                                         aCCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATa              >  1:213599/1‑150 (MQ=255)
                                                                                                                                          ccATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCTATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATaa             >  2:270614/1‑150 (MQ=255)
                                                                                                                                            aTCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATAAAc           >  2:2928957/1‑150 (MQ=255)
                                                                                                                                             tCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATAAACa          <  2:1482856/150‑1 (MQ=255)
                                                                                                                                             tCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATAAACa          <  2:1854922/150‑1 (MQ=255)
                                                                                                                                                gTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATAAACATAg       >  2:1199214/1‑150 (MQ=255)
                                                                                                                                                 tAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATAAACATAGt      <  2:3608397/150‑1 (MQ=255)
                                                                                                                                                     gTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATAAACATAGTtcat  >  1:1008883/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATAAACATAGTTCAT  >  CP043523/2059759‑2060057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑