Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 4,892 C→A R546S (CGT→AGT)  FTN78_chr0004 → Phenylalanyl‑tRNA synthetase beta chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435234,8920CA99.6% 1700.0 / NA 509R546S (CGT→AGT) FTN78_chr0004Phenylalanyl‑tRNA synthetase beta chain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (237/270);  minor base G (1/1);  total (238/271)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 509 total aligned reads displayed.

AAAAATTCCGTCGTACAGTGCGAACAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATCGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCAAATCCAGATGATATTCGTCCGATAGAAGTACC  >  CP043523/4746‑5037
                                                                                                                                                  |                                                                                                                                                 
 aaaaTTCCGTCGTACAGTGCGAACAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCa                                                                                                                                               <  1:3190803/150‑1 (MQ=255)
 aaaaTTCCGTCGTACAGTGCGAACAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCa                                                                                                                                               <  2:3388724/150‑1 (MQ=255)
   aaTTCCGTCGTACAGTGCGAACAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCaaa                                                                                                                                             >  1:3326995/1‑150 (MQ=255)
    aTTCCGTCGTACAGTGCGAACAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAac                                                                                                                                            <  1:2489386/150‑1 (MQ=255)
      tCCGTCGTACAGTGCGAACAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAacac                                                                                                                                          >  1:2409529/1‑150 (MQ=255)
       ccGTCGTACAGTGCGAACAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACt                                                                                                                                         <  1:411835/150‑1 (MQ=255)
           ngtACAGTGCGAACAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGt                                                                                                                                     >  2:2100809/2‑150 (MQ=255)
           cgtACAGTGCGAACAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGt                                                                                                                                     >  1:127486/1‑150 (MQ=255)
            gtACAGTGCGAACAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTg                                                                                                                                    >  2:304936/1‑150 (MQ=255)
             tACAGTGCGAACAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGc                                                                                                                                   <  1:2963021/150‑1 (MQ=255)
                aGTGCGAACAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCaaa                                                                                                                                >  1:1694740/1‑150 (MQ=255)
                aGTGCGAACAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCaaa                                                                                                                                >  2:4157829/1‑150 (MQ=255)
                     gAACAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGa                                                                                                                           >  1:1526624/1‑150 (MQ=255)
                      aaCAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGAt                                                                                                                          <  2:2400552/150‑1 (MQ=255)
                        cAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTc                                                                                                                        >  1:1878599/1‑150 (MQ=255)
                        cAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTc                                                                                                                        >  2:2977968/1‑150 (MQ=255)
                         aGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTcc                                                                                                                       <  1:1032130/150‑1 (MQ=255)
                         aGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTcc                                                                                                                       <  1:89454/150‑1 (MQ=255)
                          ggTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCa                                                                                                                      <  1:3228923/150‑1 (MQ=255)
                           gTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAg                                                                                                                     <  2:143775/150‑1 (MQ=255)
                            tctcGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAgg                                                                                                                    >  1:1648811/1‑150 (MQ=255)
                              tcGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGAc                                                                                                                  <  1:4343965/150‑1 (MQ=255)
                              tcGAAAGTTCAGGGCTTAATGAAGTTATTGGCTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGAc                                                                                                                  <  2:4337883/150‑1 (MQ=255)
                               cGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTCTTACTCCTGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAGCGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCTTGCAAACATGATTCCAGGACt                                                                                                                 <  2:4448232/150‑1 (MQ=255)
                                 aaaGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACttt                                                                                                               >  2:1041521/1‑150 (MQ=255)
                                 aaaGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACttt                                                                                                               <  2:1490403/150‑1 (MQ=255)
                                 aaaGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACttt                                                                                                               >  1:2040103/1‑150 (MQ=255)
                                 aaaGTACAGGGCATAATGAAGTTATTGGTTCTTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACttt                                                                                                               >  2:3542323/1‑150 (MQ=255)
                                  aaGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACtttt                                                                                                              <  2:3780465/150‑1 (MQ=255)
                                    gTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTgg                                                                                                            <  2:3072524/150‑1 (MQ=255)
                                      tCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGat                                                                                                          <  2:3710982/150‑1 (MQ=255)
                                       cAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGata                                                                                                         >  2:1417741/1‑150 (MQ=255)
                                       cAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGata                                                                                                         >  2:1233198/1‑150 (MQ=255)
                                       cAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGata                                                                                                         >  1:451913/1‑150 (MQ=255)
                                       cAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGata                                                                                                         >  2:3939166/1‑150 (MQ=255)
                                        aGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGatat                                                                                                        >  1:527043/1‑150 (MQ=255)
                                         gggCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATAtt                                                                                                       >  1:4592979/1‑150 (MQ=255)
                                          ggCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTg                                                                                                      <  1:192184/150‑1 (MQ=255)
                                          ggCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTg                                                                                                      <  2:2885900/150‑1 (MQ=255)
                                            cgtAAGGAAGTTATTGGTTATTCGCTTGAAACTCCAGAAAAAGCAACGGAATTTGTGGGTAAGCTTGAAACAACAACGCTTATGATGCAAGGGACCGAGGATGGTCAAAAACTTCGTGCAAACGTGATTCCTGAAGTTTTGGATATTTTa                                                                                                    <  2:526906/148‑1 (MQ=255)
                                              tAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTaaa                                                                                                  <  1:3064947/150‑1 (MQ=255)
                                               aaTGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAAt                                                                                                 >  2:1533394/1‑150 (MQ=255)
                                                aTGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAAtc                                                                                                <  2:2746158/150‑2 (MQ=255)
                                                 tGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTa                                                                                               >  1:75536/1‑150 (MQ=255)
                                                 agAAGTTATTAGTTATTCGCTTGTAACACCAAAAAAAGCAACTGAATTTGTGGGGCAGCTAGAAACAACAACGCTAATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGTAAACATGATTCCACGACTTTTGGATATTGTAAAtca                                                                                               <  2:4015954/149‑3 (MQ=255)
                                                  gAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTAc                                                                                              <  2:1867746/150‑1 (MQ=255)
                                                  gAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTAc                                                                                              >  1:2622209/1‑150 (MQ=255)
                                                     gTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAAt                                                                                           >  2:3634023/1‑150 (MQ=255)
                                                     gTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAAt                                                                                           <  1:4178005/150‑1 (MQ=255)
                                                     gTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAAt                                                                                           <  1:2460710/150‑1 (MQ=255)
                                                      ttATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATc                                                                                          >  1:3875904/1‑150 (MQ=255)
                                                       tATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCa                                                                                         >  1:4265701/1‑150 (MQ=255)
                                                        aTTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAg                                                                                        >  1:3363367/1‑150 (MQ=255)
                                                         ttGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGa                                                                                       <  1:1818289/150‑1 (MQ=255)
                                                              tATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGt                                                                                  <  1:277051/150‑1 (MQ=255)
                                                                ttCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTaa                                                                                <  1:4309308/150‑1 (MQ=255)
                                                                ttCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTaa                                                                                <  1:955988/150‑1 (MQ=255)
                                                                ttCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTaa                                                                                >  2:643736/1‑150 (MQ=255)
                                                                       gTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCt                                                                         <  2:892015/150‑1 (MQ=255)
                                                                       gTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCt                                                                         >  1:3648530/1‑150 (MQ=255)
                                                                        tAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTg                                                                        <  2:2300317/150‑1 (MQ=255)
                                                                             ccAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGAtgtg                                                                   >  1:315106/1‑150 (MQ=255)
                                                                             ccAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGAtgtg                                                                   >  2:284185/1‑150 (MQ=255)
                                                                               aGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGc                                                                 >  1:3525055/1‑150 (MQ=255)
                                                                                 aaaaaGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCaa                                                               >  1:2284192/1‑150 (MQ=255)
                                                                                  aaaaGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAAt                                                              <  2:3292968/150‑1 (MQ=255)
                                                                                       cAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATg                                                         >  2:1743152/1‑150 (MQ=255)
                                                                                       cAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATg                                                         <  1:991296/150‑1 (MQ=255)
                                                                                         aCTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGaa                                                       <  1:4041821/150‑1 (MQ=255)
                                                                                          cTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGaaa                                                      <  1:2333624/150‑1 (MQ=255)
                                                                                          cTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGaaa                                                      <  1:254146/150‑1 (MQ=255)
                                                                                           tGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAAt                                                     <  2:234875/150‑1 (MQ=255)
                                                                                           tGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAAt                                                     >  1:2992493/1‑150 (MQ=255)
                                                                                             aaTTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTg                                                   >  2:3209516/1‑150 (MQ=255)
                                                                                              ccttGTGGGTCAGCTTGAAACCACAGCGCTTATGATGCCAATGACCGAAGATAGTCCAACACTTCGTGCAAACATGAGTCCCGGACTTTGGGATATTGTAAATTACAAGCAAAATCATAAAAATGCTGATGTGGCAATTAATGAAATTgg                                                  >  2:2609857/3‑150 (MQ=255)
                                                                                              aTTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTgg                                                  >  2:753001/1‑150 (MQ=255)
                                                                                              aTTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACAGACGATAGTCAAACACTTCTTGCAAACATGAATAAAGGACTATTGGATATTGTAAATTACAATCAGAATCGTAAAAATGATGGTGTGGCAAATTATGAAATTga                                                  >  2:3449465/1‑149 (MQ=255)
                                                                                                 tgtgGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCaa                                               >  1:3385532/1‑150 (MQ=255)
                                                                                                  gtgGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAat                                              <  2:2559943/150‑1 (MQ=255)
                                                                                                         aGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCt                                       <  1:1756457/150‑1 (MQ=255)
                                                                                                         aGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCt                                       >  2:1798182/1‑150 (MQ=255)
                                                                                                           cTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTa                                     <  1:325516/150‑1 (MQ=255)
                                                                                                             tGAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTAcc                                   <  2:3141211/150‑1 (MQ=255)
                                                                                                              gAAACAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCa                                  <  1:3748219/150‑1 (MQ=255)
                                                                                                               aaaCAACAACGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCaa                                 <  1:1558327/150‑1 (MQ=255)
                                                                                                                      aacGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCAAATCCAga                          <  2:1120524/150‑1 (MQ=255)
                                                                                                                        cGCTTATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCAAATCCAgatg                        >  1:1164691/1‑150 (MQ=255)
                                                                                                                        cGCTTATGATACCAATGTCCTAAGATAGTCAAACACTACGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCAAATCCAgatg                        <  2:1643550/150‑1 (MQ=255)
                                                                                                                           ttATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCAAATCCAGATGata                     >  2:473478/1‑150 (MQ=255)
                                                                                                                            tATGATGCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCAAATCCAGATGatat                    >  2:4401215/1‑150 (MQ=255)
                                                                                                                             atgatgCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCAAATCCAGATGATAtt                   >  2:4303321/1‑150 (MQ=255)
                                                                                                                              tgatgCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCAAATCCAGATGATATTc                  >  1:2151405/1‑150 (MQ=255)
                                                                                                                               gatgCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCAAATCCAGATGATATTCg                 >  1:1107946/1‑150 (MQ=255)
                                                                                                                                  gCCAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCAAATCCAGATGATATTCGTcc              <  2:451913/150‑1 (MQ=255)
                                                                                                                                    cAATGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCAAATCCAGATGATATTCGTCCGa            >  2:1996388/1‑150 (MQ=255)
                                                                                                                                     aaTGACCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCAAATCCAGATGATATTCGTCCGAt           >  1:835456/1‑150 (MQ=255)
                                                                                                                                         aCCGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCTATTTATGAAATTGTCAATATTTTCTTACCAAATCCAGATGATATTCGTCCGATAGaa       <  2:3875755/150‑1 (MQ=255)
                                                                                                                                           cGAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCAAATCCAGATGATATTCGTCCGATAGAAGt     >  1:825860/1‑150 (MQ=255)
                                                                                                                                            gAAGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCAAATCCAGATGATATTCGTCCGATAGAAGTa    >  1:712766/1‑150 (MQ=255)
                                                                                                                                             aaGATAGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCAAATCCAGATGATATTCGTCCGATAGAAGTAc   <  1:2076202/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AAAAATTCCGTCGTACAGTGCGAACAGGTCTCGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTGAAACAACAACGCTTATGATGCCAATGACCGAAGATCGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATTGTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCAAATCCAGATGATATTCGTCCGATAGAAGTACC  >  CP043523/4746‑5037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑