BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,883,288	C→T	A3A (GCC→GCT)	FTN78_chr1928 →	Transposase TnpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,883,288	0	C	T	99.8%	1553.9 / NA	458	A3A (GCC→GCT)	FTN78_chr1928	Transposase TnpA
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (209/246); total (212/246)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 458 total aligned reads displayed.

CCGATTTGAACACACGACAGTCATGCTCTGTCGTGTTTTACATGTCAATCGTTCCATCAACTATAACTTTATAAACAAGAGGCCTTCGAAGCGTGAAGTAAAAAATCAACGCTTGTGAAAACACCTCCTTGAGATTTATATGAAAGCCAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCTGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATAAAACCTCGTTTTAAATCAAATAA  >  CP043523/1883141‑1883434

ccGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaa                                                                                                                                                  >  2:4145660/1‑150 (MQ=255)

   aTTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaa                                                                                                                                               >  1:2464598/1‑150 (MQ=255)

   aTTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaa                                                                                                                                               <  2:3326335/150‑1 (MQ=255)

    tttGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaaa                                                                                                                                              >  1:3050112/1‑150 (MQ=255)

    tttGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaaa                                                                                                                                              <  2:2193231/150‑1 (MQ=255)

      tGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaaaag                                                                                                                                            <  2:3239488/150‑1 (MQ=255)

       gAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaaaaga                                                                                                                                           >  1:915566/1‑150 (MQ=255)

        aaCACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaaaagaa                                                                                                                                          >  2:4561493/1‑150 (MQ=255)

        aaCACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaaaagaa                                                                                                                                          <  2:724487/150‑1 (MQ=255)

         acacacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAAt                                                                                                                                         <  1:2783746/150‑1 (MQ=255)

          cacacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAAtt                                                                                                                                        >  2:2240268/1‑150 (MQ=255)

           acacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTg                                                                                                                                       <  2:382385/150‑1 (MQ=255)

           acacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTg                                                                                                                                       >  2:1069160/1‑150 (MQ=255)

           acacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTg                                                                                                                                       <  2:2480991/150‑1 (MQ=255)

            cacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTgg                                                                                                                                      >  1:1551730/1‑150 (MQ=255)

             acGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGa                                                                                                                                     <  1:485970/150‑1 (MQ=255)

             acGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGa                                                                                                                                     <  1:4056080/150‑1 (MQ=255)

              cGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGaa                                                                                                                                    <  2:1148408/150‑1 (MQ=255)

              cGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGaa                                                                                                                                    >  1:3759987/1‑150 (MQ=255)

                aCAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACg                                                                                                                                  >  1:2627211/1‑150 (MQ=255)

                  aGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACgag                                                                                                                                >  2:351307/1‑150 (MQ=255)

                     taTGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGc                                                                                                                             <  1:547378/149‑1 (MQ=255)

                      aTGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCt                                                                                                                            <  2:3533902/150‑1 (MQ=255)

                        gCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCttt                                                                                                                          <  1:2398937/150‑1 (MQ=255)

                         ctctGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCtttt                                                                                                                         >  2:780415/1‑150 (MQ=255)

                           ctGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTaa                                                                                                                       <  1:4120857/150‑1 (MQ=255)

                                gtgtTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCa                                                                                                                  <  1:659813/150‑1 (MQ=255)

                                 tgtTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAt                                                                                                                 <  2:2764409/150‑1 (MQ=255)

                                  gtTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAACGAATTGGAACGAGAGCTTTTAAAATCAtt                                                                                                                >  1:3509923/1‑150 (MQ=255)

                                  gtTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAtt                                                                                                                >  1:1695329/1‑150 (MQ=255)

                                   ttttACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttc                                                                                                               <  1:1437984/150‑1 (MQ=255)

                                      tACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttcttc                                                                                                            <  2:622360/150‑1 (MQ=255)

                                       aCATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATtcttct                                                                                                           <  1:2860051/150‑1 (MQ=255)

                                        cATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTg                                                                                                          >  1:1048316/1‑150 (MQ=255)

                                         aTGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGc                                                                                                         <  2:3618266/150‑1 (MQ=255)

                                          tGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAGAATCAACGATTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCg                                                                                                        >  2:4485293/1‑150 (MQ=255)

                                           gTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGt                                                                                                       >  1:984844/1‑150 (MQ=255)

                                             cAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGa                                                                                                     <  2:204814/150‑1 (MQ=255)

                                              aaTCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGAt                                                                                                    >  2:1685574/1‑150 (MQ=255)

                                              aaTCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGAt                                                                                                    >  1:3967215/1‑150 (MQ=255)

                                              aaTCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGAt                                                                                                    >  2:4293252/1‑150 (MQ=255)

                                              aaTCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTACGAAGCGTGAAGTAGAAAATCAACGCTTAAGAAAATTAGTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTGTAATATCATTCTTCTGCGTGAt                                                                                                    >  2:4218836/1‑150 (MQ=255)

                                               aTCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGAtt                                                                                                   >  1:1778556/1‑150 (MQ=255)

                                                 cGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTAt                                                                                                 >  1:100141/1‑150 (MQ=255)

                                                         ctactgtaACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTaa                                                                                         >  1:1741857/7‑150 (MQ=255)

                                                         ctactgtaACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTaa                                                                                         >  1:2250968/7‑150 (MQ=255)

                                                          taCTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAat                                                                                        >  2:1841603/2‑150 (MQ=255)

                                                          taCTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAat                                                                                        >  2:1708968/2‑150 (MQ=255)

                                                           aCTGTAACTTCATGAACAAGTGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAata                                                                                       <  1:4364041/150‑1 (MQ=255)

                                                              gtaACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTAGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATAttt                                                                                    >  2:4012130/2‑150 (MQ=255)

                                                                   ttcataAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATGACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGaa                                                                               >  2:2695164/4‑150 (MQ=255)

                                                                     cataAACAAGAGGCCTTGGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAgg                                                                             <  1:4205487/149‑1 (MQ=255)

                                                                     cataAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAgg                                                                             >  1:926155/2‑150 (MQ=255)

                                                                       taAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGcc                                                                           <  2:1456487/150‑2 (MQ=255)

                                                                           cAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTAt                                                                       <  1:1984739/150‑1 (MQ=255)

                                                                           cAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTAt                                                                       <  2:2080124/150‑1 (MQ=255)

                                                                            aaGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATc                                                                      <  1:590272/150‑1 (MQ=255)

                                                                             agagGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCt                                                                     <  1:3216028/150‑1 (MQ=255)

                                                                              gagGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCtt                                                                    <  1:3781538/150‑1 (MQ=255)

                                                                                  ccTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGa                                                                <  2:2429075/150‑1 (MQ=255)

                                                                                    ttCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACt                                                              >  1:2358163/1‑150 (MQ=255)

                                                                                        aaGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTtctc                                                          >  2:3379375/1‑150 (MQ=255)

                                                                                         aGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCa                                                         >  2:525101/1‑150 (MQ=255)

                                                                                           cGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAg                                                       >  1:3389444/1‑150 (MQ=255)

                                                                                           cGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAg                                                       <  2:1993335/150‑1 (MQ=255)

                                                                                            gTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGt                                                      <  1:160535/150‑1 (MQ=255)

                                                                                             tGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTc                                                     >  2:2810535/1‑150 (MQ=255)

                                                                                             tGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTc                                                     <  1:2037514/150‑1 (MQ=255)

                                                                                              gAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCt                                                    <  1:3020942/150‑1 (MQ=255)

                                                                                                aGTAGAAAATCAACGCGTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTAt                                                  <  1:1517919/150‑1 (MQ=255)

                                                                                                   agaaaaTCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGac                                               >  2:2531771/3‑150 (MQ=255)

                                                                                                    gaaaaTCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGaca                                              <  1:2450445/149‑1 (MQ=255)

                                                                                                    gaaaaTCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGaca                                              >  1:4305859/2‑150 (MQ=255)

                                                                                                          cAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCt                                        <  1:1299600/150‑1 (MQ=255)

                                                                                                           aaCGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTa                                       >  2:3068893/1‑150 (MQ=255)

                                                                                                           aaCGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTa                                       <  1:428589/150‑1 (MQ=255)

                                                                                                            aCGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTaa                                      <  1:216065/150‑1 (MQ=255)

                                                                                                                       aattactcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCa                           <  2:4073372/145‑1 (MQ=255)

                                                                                                                        attactcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCAt                          <  2:2348879/146‑1 (MQ=255)

                                                                                                                          tactcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCAtca                        <  2:833034/148‑3 (MQ=255)

                                                                                                                           actcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAATGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCAtcca                       >  2:1288630/2‑147 (MQ=255)

                                                                                                                           actcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATcaa                       <  2:3759930/149‑1 (MQ=255)

                                                                                                                              cctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAAcc                    >  2:3952472/1‑150 (MQ=255)

                                                                                                                               ctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCGAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCt                   >  1:3874374/1‑150 (MQ=255)

                                                                                                                               ctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCt                   >  1:137164/1‑150 (MQ=255)

                                                                                                                               ctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCt                   <  1:1708968/150‑1 (MQ=255)

                                                                                                                                 tGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCttg                 <  1:3623172/150‑3 (MQ=255)

                                                                                                                                 tGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCttg                 >  2:3020812/1‑148 (MQ=255)

                                                                                                                                   agaTTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGtt               >  1:1865546/1‑150 (MQ=255)

                                                                                                                                    gaTTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGttt              <  1:497499/150‑1 (MQ=255)

                                                                                                                                     aTTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGtttt             <  1:3282772/150‑1 (MQ=255)

                                                                                                                                       ttATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTaa           >  2:3551856/1‑150 (MQ=255)

                                                                                                                                         atatGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAAt         >  1:2125067/1‑150 (MQ=255)

                                                                                                                                         atatGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAAt         >  1:1132556/1‑150 (MQ=255)

                                                                                                                                         atatGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAAt         <  1:2920898/150‑1 (MQ=255)

                                                                                                                                           atGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCa       <  2:926155/150‑1 (MQ=255)

                                                                                                                                           atGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTATGCGTGATTATGGAGTTAATATTTCTGAAGGCCGTATCTTAAGACTGCTCAAGTATATGACAACCCCTAAAATGTCAACCATCAAACCTTGTTTTATATCa       >  2:1579624/1‑150 (MQ=255)

                                                                                                                                            tGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaa      <  1:866717/150‑1 (MQ=255)

                                                                                                                                               aaGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaaata   <  2:1624250/150‑1 (MQ=255)

                                                                                                                                                aGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaaataa  >  1:2571005/1‑150 (MQ=255)

CCGATTTGAACACACGACAGTCATGCTCTGTCGTGTTTTACATGTCAATCGTTCCATCAACTATAACTTTATAAACAAGAGGCCTTCGAAGCGTGAAGTAAAAAATCAACGCTTGTGAAAACACCTCCTTGAGATTTATATGAAAGCCAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCTGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATAAAACCTCGTTTTAAATCAAATAA  >  CP043523/1883141‑1883434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`