Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,883,411 A→C I44I (ATA→ATC)  FTN78_chr1928 → Transposase TnpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,883,4110AC99.8% 1704.5 / NA 481I44I (ATA→ATC) FTN78_chr1928Transposase TnpA
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (238/242);  total (238/243)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 486 total aligned reads displayed.

CCTCCTTGAGATTTATATGAAAGCCAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCTGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATAAAACCTCGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTG  >  CP043523/1883264‑1883557
                                                                                                                                                   |                                                                                                                                                  
actcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATcaa                                                                                                                                                  <  2:3759930/149‑1 (MQ=255)
 ctcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATcaaa                                                                                                                                                 >  1:2044827/1‑150 (MQ=255)
    ctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCGAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCt                                                                                                                                              >  1:3874374/1‑150 (MQ=255)
    ctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCt                                                                                                                                              >  1:137164/1‑150 (MQ=255)
    ctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCt                                                                                                                                              <  1:1708544/150‑1 (MQ=255)
     ttGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCtt                                                                                                                                             <  2:2522027/150‑2 (MQ=255)
          aTTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGtttt                                                                                                                                        >  2:610143/1‑150 (MQ=255)
           tttATATGCAAGCTCAGCAAAGAATGGGAACGAGAGCTTTTAAAATCATTATTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGATACTCCCTAAAATGTCCACCATCAAACCTTGTATTa                                                                                                                                       >  2:3354164/1‑150 (MQ=255)
           tttATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTa                                                                                                                                       <  1:543647/150‑1 (MQ=255)
            ttATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTaa                                                                                                                                      >  1:760078/1‑150 (MQ=255)
              atatGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAAt                                                                                                                                    >  2:2224073/1‑150 (MQ=255)
                atGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCa                                                                                                                                  >  2:1595538/1‑150 (MQ=255)
                atGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCa                                                                                                                                  <  2:926155/150‑1 (MQ=255)
                 tGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaa                                                                                                                                 >  2:3070879/1‑150 (MQ=255)
                    aaGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaaata                                                                                                                              <  2:381323/150‑1 (MQ=255)
                      gctaagaaaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAAACaaataaa                                                                                                                            <  2:1551730/147‑1 (MQ=255)
                       ctaagaaaagatGTGGAACGAGAGCTTTTAGAATCATTCTTCTGCGTGATTATGGCGTTAGTATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACGCTCCCGAAAATGTCAACCATCAAACCTTGTTTTAAATCaactaaaa                                                                                                                           <  2:2341734/148‑2 (MQ=255)
                         aagaaaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAAtct                                                                                                                         <  1:610093/150‑1 (MQ=255)
                             aaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTg                                                                                                                     <  1:236825/150‑1 (MQ=255)
                             aaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTg                                                                                                                     >  1:1896786/1‑150 (MQ=255)
                             aaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTg                                                                                                                     <  2:3006590/150‑1 (MQ=255)
                              aagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGt                                                                                                                    <  2:3967215/150‑1 (MQ=255)
                              aagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGt                                                                                                                    <  1:3534460/150‑1 (MQ=255)
                                 aaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTAtt                                                                                                                 <  1:2185437/150‑1 (MQ=255)
                                 aaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTAtt                                                                                                                 <  2:984844/150‑1 (MQ=255)
                                 aaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTAtt                                                                                                                 <  1:825881/150‑1 (MQ=255)
                                   ttGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTAtttt                                                                                                               <  1:4218836/150‑1 (MQ=255)
                                   ttGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTCAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTAtttt                                                                                                               <  1:3586158/150‑1 (MQ=255)
                                        aCGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTtcttct                                                                                                          <  1:4485833/150‑1 (MQ=255)
                                        aCGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTtcttct                                                                                                          <  2:2627211/150‑1 (MQ=255)
                                           agagCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGAt                                                                                                       <  1:1665080/150‑1 (MQ=255)
                                             agCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATaa                                                                                                     >  2:1696181/1‑150 (MQ=255)
                                              gCTTTTAACATCATTCTTCTGCGTGATTATCGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTATTAAATCAAATAAATCTCCTGTGTTGTCTTCTGATAAt                                                                                                    <  2:2076147/150‑1 (MQ=255)
                                              gCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTCATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAAt                                                                                                    <  1:3773216/150‑1 (MQ=255)
                                               cTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATc                                                                                                   <  1:3268810/150‑1 (MQ=255)
                                                 tttAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTg                                                                                                 <  1:43204/150‑1 (MQ=255)
                                                 tttAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATAACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTg                                                                                                 >  2:4193712/1‑150 (MQ=255)
                                                     aaaTCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTa                                                                                             <  2:1048316/150‑1 (MQ=255)
                                                         cATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACa                                                                                         >  1:1233282/1‑150 (MQ=255)
                                                            tcttctGCGTGATTATGGCGTTAATATTTCTGAATGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAATTCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGa                                                                                      <  2:4494457/150‑1 (MQ=255)
                                                                     tGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAAccc                                                                             >  2:3435102/1‑150 (MQ=255)
                                                                     tGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAAccc                                                                             >  1:1772492/1‑150 (MQ=255)
                                                                      gATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCg                                                                            >  1:527377/1‑150 (MQ=255)
                                                                      gATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCg                                                                            >  2:289341/1‑150 (MQ=255)
                                                                      gATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCGCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCg                                                                            <  2:3559438/150‑1 (MQ=255)
                                                                       aTTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGa                                                                           >  1:1140291/1‑150 (MQ=255)
                                                                        ttATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGaa                                                                          >  1:905013/1‑150 (MQ=255)
                                                                        ttATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGaa                                                                          <  1:2103812/150‑1 (MQ=255)
                                                                        ntATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTTTTTTTTTCTGCTTAAACAAGAATTTAACCCGaa                                                                          >  2:1965135/2‑150 (MQ=255)
                                                                         tATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAAt                                                                         >  1:1469735/1‑150 (MQ=255)
                                                                            ggCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTcn                                                                      <  2:2371000/150‑2 (MQ=255)
                                                                              cGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTcccc                                                                    >  2:860538/1‑150 (MQ=255)
                                                                              cGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTcccc                                                                    <  2:100141/150‑1 (MQ=255)
                                                                               gTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCa                                                                   >  1:4465093/1‑150 (MQ=255)
                                                                                 tAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCaaa                                                                 >  1:3649050/1‑150 (MQ=255)
                                                                                  aaTATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAAt                                                                <  2:309926/150‑1 (MQ=255)
                                                                                     atTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCaa                                                             >  1:2734667/1‑150 (MQ=255)
                                                                                         cTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGttt                                                         <  1:1841603/150‑1 (MQ=255)
                                                                                         cTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGttt                                                         <  2:4303541/150‑1 (MQ=255)
                                                                                         cTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGttt                                                         >  1:665414/1‑150 (MQ=255)
                                                                                            aaGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGa                                                      >  1:1179383/1‑150 (MQ=255)
                                                                                             aGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGac                                                     >  2:4386659/1‑150 (MQ=255)
                                                                                              ggCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGaca                                                    >  1:314314/1‑150 (MQ=255)
                                                                                                ccgTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGacaac                                                  <  1:2666674/148‑1 (MQ=255)
                                                                                                      cTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGAttt                                            <  1:2981602/150‑1 (MQ=255)
                                                                                                       ttAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTc                                           >  1:1025863/1‑150 (MQ=255)
                                                                                                        tAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCa                                          >  2:766473/1‑150 (MQ=255)
                                                                                                        tAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCa                                          <  1:3354164/150‑1 (MQ=255)
                                                                                                         aaGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCAc                                         >  2:2562087/1‑150 (MQ=255)
                                                                                                           gACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACtt                                       >  2:1749471/1‑150 (MQ=255)
                                                                                                              ttCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTtata                                    <  1:3068893/150‑1 (MQ=255)
                                                                                                                ctcAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGGATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATAtc                                  >  2:4028297/1‑150 (MQ=255)
                                                                                                                  cAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATGTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATAtctc                                <  2:2140455/150‑1 (MQ=255)
                                                                                                                    aGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCta                              <  1:247327/150‑1 (MQ=255)
                                                                                                                    aGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCta                              <  2:1471038/150‑1 (MQ=255)
                                                                                                                        tATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAgg                          >  1:3206579/1‑150 (MQ=255)
                                                                                                                        tATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAgg                          >  1:1755463/1‑150 (MQ=255)
                                                                                                                         aTGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGa                         >  2:389473/1‑150 (MQ=255)
                                                                                                                          tGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAg                        <  1:3982450/150‑1 (MQ=255)
                                                                                                                          tGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAg                        >  1:2571515/1‑150 (MQ=255)
                                                                                                                           gACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTGGGACAACAGATTTCACTTATATCTCTATAGGAGc                       >  1:4012823/1‑150 (MQ=255)
                                                                                                                            acacTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCt                      <  1:2810535/150‑1 (MQ=255)
                                                                                                                             cacTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTa                     <  2:1386681/150‑1 (MQ=255)
                                                                                                                              acTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTaa                    >  1:1557423/1‑150 (MQ=255)
                                                                                                                                 cccTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATGCCCCAAATCAAGTTTGCACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCg                 <  2:684221/150‑1 (MQ=255)
                                                                                                                                  ccTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGa                >  1:2299381/1‑150 (MQ=255)
                                                                                                                                   cTAAAATGTCAACCATCGAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGAc               <  1:4170104/150‑1 (MQ=255)
                                                                                                                                   cTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGAc               <  1:2224073/150‑1 (MQ=255)
                                                                                                                                    tAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACa              <  2:4089329/150‑1 (MQ=255)
                                                                                                                                     aaaaTGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACAt             >  2:32765/1‑150 (MQ=255)
                                                                                                                                     aaaaTGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACAt             >  2:1242601/1‑150 (MQ=255)
                                                                                                                                      aaaTGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATg            <  1:344583/150‑1 (MQ=255)
                                                                                                                                       aaTGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGt           <  2:528540/150‑1 (MQ=255)
                                                                                                                                         tGTCAACGATAAAACCTTGTTTTAAATCATATAGATGTCATGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTATCCTGAATTTCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACCTGttt         <  2:1853581/150‑1 (MQ=255)
                                                                                                                                          gTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTa        >  1:516328/1‑150 (MQ=255)
                                                                                                                                             aaCCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTAtct     >  2:2800587/1‑150 (MQ=255)
                                                                                                                                             aaCCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTAtct     >  1:815287/1‑150 (MQ=255)
                                                                                                                                               ccATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGCTTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATctct   <  1:1933046/150‑1 (MQ=255)
                                                                                                                                                cATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCtg  <  2:4171796/150‑1 (MQ=255)
                                                                                                                                                cATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCtg  <  2:2276435/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCTCCTTGAGATTTATATGAAAGCCAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCTGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATAAAACCTCGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTG  >  CP043523/1883264‑1883557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑