BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,883,597	A→G	*106W (TGA→TGG)	FTN78_chr1928 →	Transposase TnpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,883,597	0	A	G	99.8%	1837.9 / NA	521	*106W (TGA→TGG)	FTN78_chr1928	Transposase TnpA
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (254/266); minor base T (1/0); total (255/266)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 524 total aligned reads displayed.

TTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGAAAAGTAAGTGATAGGATTGATGCCAAACTCGCCTGTGATACCTTAGAAATAGCTATAACTAAGAGGAAGCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTCCTTTAGAAAGTTATTAGATG  >  CP043523/1883450‑1883742

 tctGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGgaaa                                                                                                                                                >  2:1652652/1‑150 (MQ=255)

   tGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAaagt                                                                                                                                              >  1:2581460/1‑150 (MQ=255)

       aaTCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAaagtaagt                                                                                                                                          >  1:297473/1‑150 (MQ=255)

       aaTCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAaagtaagt                                                                                                                                          >  1:420970/1‑150 (MQ=255)

         tCTGATTAAAAAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGAACAACAGATTTCATTTATATCGCTAGAGGAGATAAGCGACATGTTTATATGTGTGCTATTCTTGACATCTACTCAAGGAAATGTATTACGTGGAAAGTAAGTGa                                                                                                                                        >  2:2210463/1‑150 (MQ=255)

          cTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGAt                                                                                                                                       <  2:1025863/150‑1 (MQ=255)

           tGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGAtc                                                                                                                                      >  2:741790/1‑149 (MQ=255)

             cTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGAtcga                                                                                                                                    >  1:2064535/1‑147 (MQ=255)

               tAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGaccaccg                                                                                                                                  <  1:1415718/150‑7 (MQ=255)

                aaaCAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGAtcgaatt                                                                                                                                 >  1:3262693/1‑144 (MQ=255)

                aaaCAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGAtcgaatt                                                                                                                                 <  1:4567034/150‑7 (MQ=255)

                aaaCAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGAtcgaatt                                                                                                                                 >  2:3338078/1‑144 (MQ=255)

                aaaCAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGAtcgaatt                                                                                                                                 >  2:788091/1‑144 (MQ=255)

                   cAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGAt                                                                                                                              >  2:3169708/1‑150 (MQ=255)

                   cAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGAt                                                                                                                              <  1:3435102/150‑1 (MQ=255)

                    aaGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATg                                                                                                                             <  1:2029550/150‑1 (MQ=255)

                    aaGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATg                                                                                                                             <  2:527377/150‑1 (MQ=255)

                    aaGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATg                                                                                                                             >  1:1567966/1‑150 (MQ=255)

                     aGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGc                                                                                                                            <  1:3144708/150‑1 (MQ=255)

                       aaTTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCa                                                                                                                          >  1:1843070/1‑150 (MQ=255)

                       aaTTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCa                                                                                                                          >  2:557121/1‑150 (MQ=255)

                        aTTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCaa                                                                                                                         >  1:2364014/1‑150 (MQ=255)

                         tttAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCaaa                                                                                                                        <  2:905013/150‑1 (MQ=255)

                                   aTTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAATCTCGCCtgcg                                                                                                              <  2:1187753/150‑3 (MQ=255)

                                   aTTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATAGAATTGATGACAAACTCGCCtgcg                                                                                                              >  1:1721086/1‑148 (MQ=255)

                                    ttCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGa                                                                                                             <  2:1757420/150‑1 (MQ=255)

                                          aaaTCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCtt                                                                                                       <  1:4148996/150‑1 (MQ=255)

                                           aaTCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTa                                                                                                      <  2:840803/150‑1 (MQ=255)

                                           aaTCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTa                                                                                                      <  2:2734667/150‑1 (MQ=255)

                                                    tGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCt                                                                                             <  1:3910889/150‑1 (MQ=255)

                                                           caGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATa                                                                                      >  1:925331/1‑150 (MQ=255)

                                                             gATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAag                                                                                    >  2:2433436/1‑150 (MQ=255)

                                                             gATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAag                                                                                    <  2:2827031/150‑1 (MQ=255)

                                                             gATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAag                                                                                    <  2:1116862/150‑1 (MQ=255)

                                                               tttCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAagag                                                                                  <  2:4574871/150‑1 (MQ=255)

                                                                ttCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAgg                                                                                 <  1:766473/150‑1 (MQ=255)

                                                                 tCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGa                                                                                >  2:3282090/1‑150 (MQ=255)

                                                                   aCTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGaat                                                                              >  1:2287223/1‑149 (MQ=255)

                                                                   aCTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGaat                                                                              >  1:4036578/1‑149 (MQ=255)

                                                                     ttATATCTCTATAGGAGGTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATcc                                                                            <  1:4179425/150‑1 (MQ=255)

                                                                      tataTCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCt                                                                           <  1:4280058/150‑1 (MQ=255)

                                                                      tataTCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCt                                                                           <  2:3403595/150‑1 (MQ=255)

                                                                      tataTCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCt                                                                           >  2:2919973/1‑150 (MQ=255)

                                                                      tataTCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCt                                                                           <  2:1470108/150‑1 (MQ=255)

                                                                      tataTCTCTATAGGAGCTAAGAGATATGTTTATCTGTGTGCTATTATTGAAGTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATAGAATTGATGCCAAACTCGGTTGTGACAGTTTAGAAAGATTTACAAATAAGAGGaatgct                                                                           >  2:1982354/1‑146 (MQ=255)

                                                                          tctcTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAgg                                                                       <  1:497314/150‑1 (MQ=255)

                                                                          tctcTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAgg                                                                       >  1:1599230/1‑150 (MQ=255)

                                                                              tataGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAAcc                                                                   >  1:1353481/1‑150 (MQ=255)

                                                                               ataGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCa                                                                  >  2:1828094/1‑150 (MQ=255)

                                                                                 aGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAAt                                                                >  2:3595634/1‑150 (MQ=255)

                                                                                 aGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAAt                                                                <  2:3800999/150‑1 (MQ=255)

                                                                                 aGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAAt                                                                >  1:1169659/1‑150 (MQ=255)

                                                                                  ggAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAAtt                                                               <  1:1942234/150‑1 (MQ=255)

                                                                                  ggAGCTAAGCGACATGTTTATCTCTGTGCTATTCATGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAAtt                                                               <  2:2299381/150‑1 (MQ=255)

                                                                                     gCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATc                                                            <  2:3142263/150‑1 (MQ=255)

                                                                                       tAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCtt                                                          >  2:3052192/1‑150 (MQ=255)

                                                                                       tAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCtt                                                          >  2:2618062/1‑150 (MQ=255)

                                                                                        aaGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCttt                                                         <  2:1523517/150‑1 (MQ=255)

                                                                                          gCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCa                                                       >  2:3640156/1‑150 (MQ=255)

                                                                                           cGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCAt                                                      >  2:3524897/1‑150 (MQ=255)

                                                                                            gACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCAtt                                                     >  1:675915/1‑150 (MQ=255)

                                                                                              cATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCg                                                   <  1:711900/150‑1 (MQ=255)

                                                                                                tGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAACTGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGa                                                 <  1:4487247/150‑1 (MQ=255)

                                                                                                 gTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGAt                                                <  2:657048/150‑1 (MQ=255)

                                                                                                  tttATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATc                                               <  1:3524897/150‑1 (MQ=255)

                                                                                                   ttATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCa                                              <  2:1882973/150‑1 (MQ=255)

                                                                                                   ttATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCa                                              <  1:3877214/150‑1 (MQ=255)

                                                                                                     aTCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAg                                            <  1:302879/150‑1 (MQ=255)

                                                                                                     aTCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAg                                            >  2:1778588/1‑150 (MQ=255)

                                                                                                     aTCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAg                                            >  1:1005242/1‑150 (MQ=255)

                                                                                                        tctGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGaa                                         <  2:2059849/150‑1 (MQ=255)

                                                                                                         ctGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAg                                        <  2:1144685/150‑1 (MQ=255)

                                                                                                          tgtgCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGc                                       <  2:1353481/150‑1 (MQ=255)

                                                                                                            tgCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCGTTCATTCGGATCAAGGAAGCCa                                     >  2:4290282/1‑150 (MQ=255)

                                                                                                              cTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAAt                                   <  2:1577882/150‑1 (MQ=255)

                                                                                                              cTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAAt                                   >  1:3798722/1‑150 (MQ=255)

                                                                                                              cTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAAt                                   <  1:2673663/150‑1 (MQ=255)

                                                                                                                  tCTTGACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTaa                               <  2:1414378/150‑1 (MQ=255)

                                                                                                                      gACCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCa                           >  2:4390950/1‑150 (MQ=255)

                                                                                                                       aCCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCTTTCGGATCAAGGAAGCCAATTTAAATCAg                          <  2:1590499/150‑1 (MQ=255)

                                                                                                                       aCCTCTACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAg                          <  1:24962/150‑1 (MQ=255)

                                                                                                                         ctctACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCt                        >  1:2834852/1‑150 (MQ=255)

                                                                                                                           ctACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTc                      >  2:3932170/1‑150 (MQ=255)

                                                                                                                            tACTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTcc                     >  1:2177059/1‑150 (MQ=255)

                                                                                                                             aCTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTCCt                    >  1:3618888/1‑150 (MQ=255)

                                                                                                                              cTCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTCCtt                   >  2:3227752/1‑150 (MQ=255)

                                                                                                                               tCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGACTCCTAAGGAACCAATTAACTTTCATTCGCATCAAGGAAGCCAATTTAAATCAGCTTCCttt                  >  2:4102185/1‑150 (MQ=255)

                                                                                                                               tCAAGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTCCttt                  >  2:618561/1‑150 (MQ=255)

                                                                                                                                 aaGGAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTCCTTTAg                <  1:3362873/150‑1 (MQ=255)

                                                                                                                                   ggAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTCCTTTAGaa              >  1:588498/1‑150 (MQ=255)

                                                                                                                                   ggAAATGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTCCTTTAGaa              >  2:3102886/1‑150 (MQ=255)

                                                                                                                                     aaaTGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTCCTTTAGAAAg            >  2:2551134/1‑150 (MQ=255)

                                                                                                                                        tGTATTGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTCCTTTAGAAAGtta         >  1:618550/1‑150 (MQ=255)

                                                                                                                                           aTTGCCTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTCCTTTAGAAAGttatta      <  2:1721086/150‑1 (MQ=255)

                                                                                                                                            ttGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTCCTTTAGAAAGTTATTAg     >  2:403065/1‑150 (MQ=255)

                                                                                                                                             tGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTCCTTTAGAAAGTTATTAGa    <  2:445790/150‑1 (MQ=255)

                                                                                                                                             tGCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTCCTTTAGAAAGTTATTAGa    >  1:828673/1‑150 (MQ=255)

                                                                                                                                              gCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTCCTTTAGAAAGTTATTAGAt   <  1:2788137/150‑1 (MQ=255)

                                                                                                                                              gCGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTCCTTTAGAAAGTTATTAGAt   >  1:1389592/1‑150 (MQ=255)

                                                                                                                                               cGTGGAAAGTAAGTGATCGAATTGATGCCAAACTCGCCTGCGACACCTTAGAAATAGCTATAAATAAGAGGAATCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTCCTTTAGAAAGTTATTAGATg  <  1:1653316/150‑1 (MQ=255)

TTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCTCTGTGCTATTCTTGACCTCTACTCAAGGAAATGTATTGCGTGAAAAGTAAGTGATAGGATTGATGCCAAACTCGCCTGTGATACCTTAGAAATAGCTATAACTAAGAGGAAGCCTAAGGAACCAATTATCTTTCATTCGGATCAAGGAAGCCAATTTAAATCAGCTTCCTTTAGAAAGTTATTAGATG  >  CP043523/1883450‑1883742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`