Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,885,578 G→C L420L (CTG→CTC)  FTN78_chr1930 → Putative UV‑damage repair protein UvrX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,885,5780GC99.4% 1877.1 / NA 539L420L (CTG→CTC) FTN78_chr1930Putative UV‑damage repair protein UvrX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (243/293);  minor base A (2/1);  total (245/294)
Fisher's exact test for biased strand distribution p-value = 5.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 547 total aligned reads displayed.

GGTTTCTCGCAATATCGAAGCAACGCAAAACACAAAGGAATTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATTAGACAAATAGGGATAAGCGGGAATCAACTTTCTGATAGTTCTGTTAAACAACTGTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACATTCGATTTTCTATCTATACAAAAAGCAAGTAGTTTATCCGAGGGATCACGTGTCATTTATCG  >  CP043523/1885431‑1885724
                                                                                                                                                   |                                                                                                                                                  
 gTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAActctct                                                                                                                                                 <  1:3361612/150‑1 (MQ=255)
  tttCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCtt                                                                                                                                                <  2:319671/150‑1 (MQ=255)
          aaTATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGaa                                                                                                                                        >  2:767593/1‑150 (MQ=255)
           atatTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGaaa                                                                                                                                       >  1:2245904/1‑150 (MQ=255)
                gAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGtt                                                                                                                                  >  1:3814427/1‑150 (MQ=255)
                 aaGCCACGCAAATCACAAAGGAGTTGCAAGGCCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTc                                                                                                                                 <  1:1111435/150‑1 (MQ=255)
                 aaGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTc                                                                                                                                 <  2:796830/150‑1 (MQ=255)
                  agccaCGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCa                                                                                                                                <  1:131986/146‑1 (MQ=255)
                  aGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCa                                                                                                                                >  1:448335/1‑150 (MQ=255)
                    ccaCGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAg                                                                                                                              <  2:3125803/148‑1 (MQ=255)
                     caCGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGa                                                                                                                             >  2:1761591/2‑150 (MQ=255)
                       cGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGaaa                                                                                                                           >  2:912550/1‑150 (MQ=255)
                       cGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGaaa                                                                                                                           <  2:1492381/150‑1 (MQ=255)
                         cAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAAt                                                                                                                         <  1:368959/150‑1 (MQ=255)
                          naaaCACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATc                                                                                                                        >  2:3251548/2‑150 (MQ=255)
                          aaaaCACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATc                                                                                                                        >  2:649543/1‑150 (MQ=255)
                           aaaCACAAAGGGGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCGAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAAGCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCa                                                                                                                       <  1:2563798/150‑1 (MQ=255)
                           aaaCACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCa                                                                                                                       <  1:2348347/150‑1 (MQ=255)
                           aaaCACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCa                                                                                                                       >  1:2649564/1‑150 (MQ=255)
                           aaaCACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCa                                                                                                                       <  1:2911420/150‑1 (MQ=255)
                            aaCACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCaa                                                                                                                      >  1:2023103/1‑150 (MQ=255)
                              cacaAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAAc                                                                                                                    <  1:2201633/150‑1 (MQ=255)
                                caAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACta                                                                                                                  <  1:653753/150‑1 (MQ=255)
                                 aaaGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTAACAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCTAATTTCTGATAGTTCTGTTAAACAACTATCTTTATTTGAAAGTGTTCAAGAaaataaaaataa                                                                                                                 >  1:3987205/1‑150 (MQ=255)
                                  aaGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACtaat                                                                                                                <  1:3575198/150‑1 (MQ=255)
                                   aGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACtaata                                                                                                               <  2:849526/150‑1 (MQ=255)
                                    ggAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACtaataa                                                                                                              <  2:3933851/150‑1 (MQ=255)
                                     gagttGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATaaa                                                                                                             <  1:2785862/147‑1 (MQ=255)
                                       gttGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATaaaaa                                                                                                           <  1:897535/149‑1 (MQ=255)
                                       gttGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATaaaaa                                                                                                           <  2:2702382/149‑1 (MQ=255)
                                         tGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGc                                                                                                         >  1:1243191/1‑150 (MQ=255)
                                           cAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCaa                                                                                                       <  2:2845887/150‑1 (MQ=255)
                                            aaGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAg                                                                                                      >  1:2154046/1‑150 (MQ=255)
                                             aGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGa                                                                                                     <  1:3301700/150‑1 (MQ=255)
                                             aGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGa                                                                                                     >  1:3117088/1‑150 (MQ=255)
                                                ccTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATc                                                                                                  >  1:3154034/1‑150 (MQ=255)
                                                ccTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATc                                                                                                  <  1:4078899/150‑1 (MQ=255)
                                                   cGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACt                                                                                               <  2:3225205/150‑1 (MQ=255)
                                                   cGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACt                                                                                               >  2:156640/1‑150 (MQ=255)
                                                     cAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTcc                                                                                             >  1:1702660/1‑150 (MQ=255)
                                                     cAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTcc                                                                                             <  2:3575318/150‑1 (MQ=255)
                                                     cAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTcc                                                                                             <  1:1742888/150‑1 (MQ=255)
                                                            tttATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGc                                                                                      >  1:1538072/1‑150 (MQ=255)
                                                            tttATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGc                                                                                      >  2:557114/1‑150 (MQ=255)
                                                                   cGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATtgat                                                                               <  2:2745743/150‑1 (MQ=255)
                                                                   cGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATtgat                                                                               <  2:3045861/150‑1 (MQ=255)
                                                                   cGTGAAAAGTACCAAGGGCGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTCTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATCAAAAGCAAGAATCACTCCAAAAAGCTATtgat                                                                               <  2:4359882/150‑1 (MQ=255)
                                                                        aaaGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGAt                                                                          <  1:489733/150‑1 (MQ=255)
                                                                        aaaGTACCAAGGGGGAGCGATAAGACAAATAGGGAAAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGAt                                                                          >  2:4279793/1‑150 (MQ=255)
                                                                         aaGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGAtt                                                                         >  2:3487955/1‑150 (MQ=255)
                                                                         aaGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGAtt                                                                         >  1:2072186/1‑150 (MQ=255)
                                                                          aGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTa                                                                        <  2:1268874/150‑1 (MQ=255)
                                                                          aGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGAGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTa                                                                        <  2:3458310/150‑1 (MQ=255)
                                                                            tACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTaga                                                                      <  1:2973535/150‑1 (MQ=255)
                                                                            tACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTaga                                                                      <  1:1644178/150‑1 (MQ=255)
                                                                             aCCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTagag                                                                     >  2:1676783/1‑150 (MQ=255)
                                                                               cAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGaa                                                                   <  1:1581964/150‑1 (MQ=255)
                                                                                aaGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGaaa                                                                  >  2:396618/1‑150 (MQ=255)
                                                                                aaGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGaaa                                                                  <  1:1818438/150‑1 (MQ=255)
                                                                                 aGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAAc                                                                 >  1:563755/1‑150 (MQ=255)
                                                                                  gggggAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAAct                                                                <  2:3668630/150‑2 (MQ=255)
                                                                                  gggggAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTATCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAAct                                                                <  2:1639182/150‑2 (MQ=255)
                                                                                   ggggAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAAGCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAActt                                                               >  2:247132/1‑148 (MQ=255)
                                                                                    gggAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACttt                                                              <  2:1866640/150‑1 (MQ=255)
                                                                                     ggAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTc                                                             <  2:1120686/150‑1 (MQ=255)
                                                                                     ggAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTc                                                             <  1:3509622/150‑1 (MQ=255)
                                                                                          gATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGAttt                                                        >  1:4216683/1‑150 (MQ=255)
                                                                                          gATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGAttt                                                        >  1:718567/1‑150 (MQ=255)
                                                                                             aagccAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAGATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGAtcttct                                                     >  2:3822508/5‑150 (MQ=255)
                                                                                             aaGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTtct                                                     >  2:478070/2‑150 (MQ=255)
                                                                                              aGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTtctt                                                    <  2:2517613/150‑2 (MQ=255)
                                                                                              aGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTtctt                                                    >  1:387151/1‑149 (MQ=255)
                                                                                               gACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTtcttt                                                   <  2:2662308/150‑3 (MQ=255)
                                                                                                  aaaTAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTtctttcaa                                                >  2:3344254/1‑145 (MQ=255)
                                                                                                    aTAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCaata                                              >  2:3403553/1‑150 (MQ=255)
                                                                                                        ggATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATAAGAGAAACTTTCGATTTTCTTTCAATACaaa                                          >  2:1067554/1‑150 (MQ=255)
                                                                                                         gATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACaaaa                                         >  2:2070253/1‑150 (MQ=255)
                                                                                                         gATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACaaaa                                         <  2:448335/150‑1 (MQ=255)
                                                                                                         gATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACaaaa                                         >  1:783297/1‑150 (MQ=255)
                                                                                                          aTAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACaaaag                                        <  1:4038392/150‑2 (MQ=255)
                                                                                                             aGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCa                                     >  1:269899/1‑150 (MQ=255)
                                                                                                               cggaaaTCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAag                                   <  1:3251548/146‑1 (MQ=255)
                                                                                                               cGGAAATCAACTTTCTGATATTGCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAATAAAATCAAACTAATAAACAGCAAGCATCACTCCAAAAAGCTATTGATGCGATTAGAGAACCTTTCGATTTTCTTTCAATACAAAAGGCAag                                   <  2:2403788/150‑1 (MQ=255)
                                                                                                                 gaaaTCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAagta                                 <  1:100714/148‑1 (MQ=255)
                                                                                                                   aaTCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAagtagt                               >  2:1355260/1‑150 (MQ=255)
                                                                                                                    aTCAACTTTCTGATAGTTCAGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGtt                              <  2:2006658/150‑1 (MQ=255)
                                                                                                                      cAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTa                            <  1:4352698/150‑1 (MQ=255)
                                                                                                                         cTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATcc                         <  2:2247285/150‑1 (MQ=255)
                                                                                                                           ttCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGa                       <  1:1915174/150‑1 (MQ=255)
                                                                                                                             cTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAgg                     >  2:2929056/1‑150 (MQ=255)
                                                                                                                               gATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAggat                   <  1:1397988/150‑3 (MQ=255)
                                                                                                                                aTAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAggatt                  <  2:215144/150‑4 (MQ=255)
                                                                                                                                aTAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGACAAGTAGTTTATCCGAggatt                  >  2:2312512/1‑147 (MQ=255)
                                                                                                                                  aGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAggattca                >  2:2206480/1‑145 (MQ=255)
                                                                                                                                      cTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGACTCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACgtg            <  1:2846717/150‑1 (MQ=255)
                                                                                                                                      cTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACgtg            <  2:1014126/150‑1 (MQ=255)
                                                                                                                                          tAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCAt        <  1:619084/150‑1 (MQ=255)
                                                                                                                                          tAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGACAAGTAGTTTATCCGAGGATTCACGTGTCAt        >  1:247472/1‑150 (MQ=255)
                                                                                                                                             acaacTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTa     >  2:408549/1‑150 (MQ=255)
                                                                                                                                                acTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGAGTAGAGAGACTGTCGATTTTCTTTCAATACAAAAGGCAAGAAGTTTATCCGAGGATTCACGTGTCATTTATCg  >  2:4126073/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTTTCTCGCAATATCGAAGCAACGCAAAACACAAAGGAATTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATTAGACAAATAGGGATAAGCGGGAATCAACTTTCTGATAGTTCTGTTAAACAACTGTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACATTCGATTTTCTATCTATACAAAAAGCAAGTAGTTTATCCGAGGGATCACGTGTCATTTATCG  >  CP043523/1885431‑1885724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑