Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,885,677 T→A S453S (TCT→TCA)  FTN78_chr1930 → Putative UV‑damage repair protein UvrX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,885,6770TA99.8% 1503.0 / NA 493S453S (TCT→TCA) FTN78_chr1930Putative UV‑damage repair protein UvrX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (267/225);  minor base C (0/1);  total (267/226)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 497 total aligned reads displayed.

AATAGGGATAAGCGGGAATCAACTTTCTGATAGTTCTGTTAAACAACTGTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACATTCGATTTTCTATCTATACAAAAAGCAAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGG  >  CP043523/1885530‑1885808
                                                                                                                                                   |                                                                                                                                   
 aTAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCaata                                                                                                                                  <  2:2797919/150‑1 (MQ=255)
 aTAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCaata                                                                                                                                  >  2:3406502/1‑150 (MQ=255)
  tAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATAc                                                                                                                                 <  1:2814081/150‑1 (MQ=255)
      gATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACaaaa                                                                                                                             <  2:448335/150‑1 (MQ=255)
       aTAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACaaaag                                                                                                                            >  1:3138913/1‑149 (MQ=255)
            cggaaaTCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAag                                                                                                                       <  1:3251548/146‑1 (MQ=255)
            cGGAAATCAACTTTCTGATATTGCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAATAAAATCAAACTAATAAACAGCAAGCATCACTCCAAAAAGCTATTGATGCGATTAGAGAACCTTTCGATTTTCTTTCAATACAAAAGGCAag                                                                                                                       <  2:2403788/150‑1 (MQ=255)
             ggaaaTCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAagt                                                                                                                      >  1:597055/4‑150 (MQ=255)
              gaaaTCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAagta                                                                                                                     <  1:100714/148‑1 (MQ=255)
              gaaaTCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAagta                                                                                                                     <  2:1030650/148‑1 (MQ=255)
              gaaaTCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAagta                                                                                                                     <  2:4592150/148‑1 (MQ=255)
                  tCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGttt                                                                                                                 <  1:2851671/150‑1 (MQ=255)
                    aaCTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTAt                                                                                                               >  2:1213814/1‑150 (MQ=255)
                      cTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATcc                                                                                                             <  1:3509988/150‑1 (MQ=255)
                      cTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATcc                                                                                                             <  1:1811730/150‑1 (MQ=255)
                        ttCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGa                                                                                                           <  1:1915174/150‑1 (MQ=255)
                          cTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAgg                                                                                                         >  1:2400796/1‑150 (MQ=255)
                          cTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAgg                                                                                                         >  2:522726/1‑150 (MQ=255)
                          cTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAgg                                                                                                         <  1:673469/150‑1 (MQ=255)
                           tGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAgga                                                                                                        >  2:43972/1‑149 (MQ=255)
                            gATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAggat                                                                                                       <  1:1790153/150‑3 (MQ=255)
                             aTAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAggatt                                                                                                      >  2:4116284/1‑147 (MQ=255)
                               aGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAggattca                                                                                                    >  2:2206480/1‑145 (MQ=255)
                                gTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATCAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAggattcac                                                                                                   >  2:4050823/1‑144 (MQ=255)
                                gTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAggattcac                                                                                                   <  2:2154046/150‑7 (MQ=255)
                                gTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAggattcac                                                                                                   >  1:2687180/1‑144 (MQ=255)
                                 ttCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAggattcacg                                                                                                  <  1:1085124/150‑8 (MQ=255)
                                  tCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACgt                                                                                                 >  2:815400/1‑150 (MQ=255)
                                    tGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACgtgt                                                                                               <  1:767593/150‑1 (MQ=255)
                                       tAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGACAAGTAGTTTATCCGAGGATTCACGTGTCAt                                                                                            >  1:247472/1‑150 (MQ=255)
                                        aaaCAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCAtt                                                                                           <  2:2065229/150‑1 (MQ=255)
                                         aacaacTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTACAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCAttt                                                                                          <  2:1605548/150‑1 (MQ=255)
                                           caacTCTCTTTATTTGAAATTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTAt                                                                                        <  1:3631873/150‑1 (MQ=255)
                                           caacTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTAt                                                                                        >  1:3988812/1‑150 (MQ=255)
                                            aacTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATc                                                                                       <  1:2163852/150‑1 (MQ=255)
                                             actctCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCg                                                                                      <  1:347128/146‑1 (MQ=255)
                                             acTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCg                                                                                      <  2:3471528/150‑1 (MQ=255)
                                              ctctCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCgg                                                                                     >  1:3600395/4‑150 (MQ=255)
                                                ctCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGaa                                                                                   >  1:3831439/2‑150 (MQ=255)
                                                  cTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACa                                                                                 >  2:2615835/1‑150 (MQ=255)
                                                  cTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACa                                                                                 <  2:3340799/150‑1 (MQ=255)
                                                   tttATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACaa                                                                                >  1:1556221/1‑150 (MQ=255)
                                                      aTTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACt                                                                             >  2:912623/1‑150 (MQ=255)
                                                      aTTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACt                                                                             <  1:2533746/150‑1 (MQ=255)
                                                       tttGGAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTACAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGTAACAAtctc                                                                            <  2:3825523/150‑1 (MQ=255)
                                                          gAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCAtt                                                                         >  1:1466626/1‑150 (MQ=255)
                                                            aaGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTgg                                                                       >  1:535263/1‑150 (MQ=255)
                                                            aaGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTgg                                                                       >  1:3349853/1‑150 (MQ=255)
                                                             aGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGa                                                                      <  2:2918393/150‑1 (MQ=255)
                                                             aGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGa                                                                      <  2:4134316/150‑1 (MQ=255)
                                                                gtTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGt                                                                   <  2:692537/150‑1 (MQ=255)
                                                                gtTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGt                                                                   >  2:634304/1‑150 (MQ=255)
                                                                  tCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCa                                                                 >  1:2342720/1‑150 (MQ=255)
                                                                    aaGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATg                                                               >  1:1294786/1‑150 (MQ=255)
                                                                      gAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGAATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATgca                                                             >  2:2680094/1‑150 (MQ=255)
                                                                       aaaaTCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATgcag                                                            <  1:2578711/150‑1 (MQ=255)
                                                                        aaaTCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGAAGAGATAAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGAGTCATATATCGGAACAAACTCATTGGAGGTCATgcagc                                                           >  2:2982638/1‑150 (MQ=255)
                                                                         aaTCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATgcagca                                                          >  2:1849910/1‑150 (MQ=255)
                                                                             aaaCTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTc                                                      <  2:3117088/150‑1 (MQ=25)
                                                                                 taataaAAAGCAAGAATCACTCCAAAAAGCTATTGATGACATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGa                                                  >  1:2757618/1‑150 (MQ=18)
                                                                                  aataaAAAGCAAGAATCACTCCAAAAAGCTATTGACGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTAACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGAc                                                 >  2:4279452/1‑150 (MQ=18)
                                                                                    taaAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACaa                                               >  2:3539955/1‑150 (MQ=25)
                                                                                      aaaaGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAagg                                             <  1:3937453/150‑1 (MQ=25)
                                                                                           cAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAaggaggta                                        >  1:1877727/1‑148 (MQ=25)
                                                                                            aaGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGTCAAGGAGGtaa                                       >  1:9469/1‑150 (MQ=21)
                                                                                             aGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGtaaa                                      >  1:2084705/1‑150 (MQ=21)
                                                                                              gAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGtaaaa                                     >  1:4043717/1‑150 (MQ=25)
                                                                                                 tCACTCCAAAAAGCTATTGATGAGATTAGAGCAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGAt                                  >  2:2754092/1‑150 (MQ=21)
                                                                                                  cACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATg                                 >  1:2026825/1‑150 (MQ=25)
                                                                                                   aCTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGt                                >  1:1709647/1‑150 (MQ=21)
                                                                                                   aCTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGt                                >  1:877348/1‑150 (MQ=21)
                                                                                                    cTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTc                               <  1:43972/150‑1 (MQ=21)
                                                                                                    cTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTc                               >  2:3767857/1‑150 (MQ=21)
                                                                                                          aaaGCTTTTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGCCAAGGAGGTAAAAGATGTCAGTTGa                         <  2:3931765/150‑1 (MQ=18)
                                                                                                           aaGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGAc                        >  2:3053288/1‑150 (MQ=21)
                                                                                                           aaGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGAc                        <  2:3190389/150‑1 (MQ=21)
                                                                                                            agcaaaagatgaGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGAcc                       >  2:1552188/8‑150 (MQ=18)
                                                                                                            aGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGAcc                       <  1:4179128/150‑1 (MQ=21)
                                                                                                             gCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCg                      <  2:1702660/150‑1 (MQ=21)
                                                                                                              cTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGt                     >  2:1439099/1‑150 (MQ=21)
                                                                                                              cTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGt                     <  2:1087628/150‑1 (MQ=21)
                                                                                                               tATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGtt                    >  2:1348132/1‑150 (MQ=21)
                                                                                                               tATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGtt                    <  1:168936/150‑1 (MQ=18)
                                                                                                                aTTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTc                   >  1:2274933/1‑150 (MQ=21)
                                                                                                                aTTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTc                   <  2:597055/150‑1 (MQ=21)
                                                                                                                 ttGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCa                  >  1:2990435/1‑150 (MQ=21)
                                                                                                                  tgatgaGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCTGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCat                 >  2:3255590/1‑150 (MQ=18)
                                                                                                                   gatgaGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCata                <  1:1587385/150‑1 (MQ=21)
                                                                                                                    gtgaGATTAGAGGATCTTTCGATTTGCTTTCAATACAAGAGGCGAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAGACTCATTGGAGGTGATGCAGCCAGTCAAGACAAGGAGGTAAAGGGTGTCAGTTGACCGTTCgtac               <  2:1994234/149‑2 (MQ=255)
                                                                                                                        gaTTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAgcc           <  2:225376/150‑3 (MQ=18)
                                                                                                                         aTTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCatacagccc          >  1:749938/1‑144 (MQ=18)
                                                                                                                         aTTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGccc          <  2:2472348/150‑1 (MQ=18)
                                                                                                                            agagAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTa       <  2:293772/150‑1 (MQ=18)
                                                                                                                              agaAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTatg     >  1:262479/1‑148 (MQ=18)
                                                                                                                               gaAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGa    >  1:4543473/1‑150 (MQ=18)
                                                                                                                               gaAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGa    <  1:4325483/150‑1 (MQ=18)
                                                                                                                               gaAACTTTCGATTTTCTTGCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTatgc    <  2:3138913/150‑4 (MQ=18)
                                                                                                                                aaaCTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTatgaa   >  1:3763370/1‑146 (MQ=18)
                                                                                                                                aaaCTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGaa   <  2:1525688/150‑2 (MQ=18)
                                                                                                                                 aactttCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGaag  <  1:1213814/146‑3 (MQ=18)
                                                                                                                                                   |                                                                                                                                   
AATAGGGATAAGCGGGAATCAACTTTCTGATAGTTCTGTTAAACAACTGTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACATTCGATTTTCTATCTATACAAAAAGCAAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGG  >  CP043523/1885530‑1885808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑