BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,885,798	T→C	S8S (AGT→AGC)	FTN78_chr1931 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,885,798	0	T	C	99.7%	619.9 / NA	330	S8S (AGT→AGC)	FTN78_chr1931	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (147/181); total (148/182)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 334 total aligned reads displayed.

ATTAGAGAAACATTCGATTTTCTATCTATACAAAAAGCAAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTATTAG‑GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATT  >  CP043523/1885651‑1885929

 ttAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGcccc                                                                                                                                   <  2:46038/150‑1 (MQ=18)

  tAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCt                                                                                                                                  <  2:1348846/150‑1 (MQ=18)

  tAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCt                                                                                                                                  <  1:2483246/150‑1 (MQ=18)

  tAGAGAAACGTTGTATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATGCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCt                                                                                                                                  <  2:632002/150‑1 (MQ=18)

   agagAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTa                                                                                                                                 <  2:3267007/150‑1 (MQ=18)

      gaAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGa                                                                                                                              >  1:4543473/1‑150 (MQ=18)

       aaaCTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTCAAAGATGTCAGTTGACCGTTCATACAGCCCCTatgaa                                                                                                                             >  2:3978347/1‑146 (MQ=17)

       aaaCTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTatgaa                                                                                                                             >  1:1781326/1‑146 (MQ=18)

        aaCTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGaag                                                                                                                            >  2:815573/1‑148 (MQ=17)

                                  aggCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCCTTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAggg                                                                                                 >  2:3747436/3‑149 (MQ=14)

                                       agtagtTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGgatgat                                                                                            >  1:530123/1‑150 (MQ=14)

                                         tagtTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGgatgatta                                                                                          <  2:783297/150‑3 (MQ=14)

                                          agtTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGaa                                                                                         <  1:3746494/150‑1 (MQ=9)

                                            tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAAt                                                                                       <  1:4116284/150‑1 (MQ=9)

                                               aTCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATggg                                                                                    >  2:3446173/1‑150 (MQ=14)

                                                   gaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGgcgc                                                                                >  1:4305185/7‑150 (MQ=14)

                                                    aggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                               <  1:4204673/145‑1 (MQ=9)

                                                     ggattCACGTGTCATTTATCTGAACAAACGCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGAGGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCAt                                                                              <  2:1950454/146‑1 (MQ=9)

                                                     ggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCAt                                                                              <  2:277568/146‑1 (MQ=14)

                                                      gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCAtt                                                                             >  1:3040287/4‑150 (MQ=14)

                                                       attCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCAttt                                                                            <  1:294148/148‑1 (MQ=14)

                                                          cACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCg                                                                         >  2:1124930/1‑150 (MQ=14)

                                                          cACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCg                                                                         <  2:2604310/150‑1 (MQ=14)

                                                          cACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCg                                                                         >  1:1010248/1‑150 (MQ=14)

                                                           aCGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGa                                                                        <  2:3922191/150‑1 (MQ=14)

                                                            cGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGAc                                                                       >  1:269839/1‑150 (MQ=14)

                                                                 cATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTggag                                                                  >  2:1485147/1‑148 (MQ=14)

                                                                  aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGgaga                                                                 <  1:2417155/150‑1 (MQ=9)

                                                                  aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGgaga                                                                 >  2:3115784/1‑150 (MQ=14)

                                                                   tttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaa                                                                >  1:4341455/1‑150 (MQ=9)

                                                                   tttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaa                                                                <  1:2615835/150‑1 (MQ=14)

                                                                     tATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaact                                                              >  2:715613/1‑148 (MQ=9)

                                                                      aTCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactc                                                             >  2:1172582/1‑147 (MQ=11)

                                                                      aTCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactc                                                             >  2:656931/1‑147 (MQ=14)

                                                                         ggAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                          <  1:641939/150‑7 (MQ=9)

                                                                         ggAACAAACGCATTGGAGGTCATGAAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTGTGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                          >  2:3834014/1‑144 (MQ=9)

                                                                          gAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacgg                                                         <  2:3600395/150‑8 (MQ=9)

                                                                          gAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacgg                                                         <  1:3668067/150‑8 (MQ=9)

                                                                          gAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacgg                                                         >  2:3038851/1‑143 (MQ=9)

                                                                                     ttGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAAt                                              >  2:3561894/1‑150 (MQ=2)

                                                                                      tGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATa                                             >  2:3014202/1‑150 (MQ=2)

                                                                                       ggAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATAc                                            >  1:4071851/1‑150 (MQ=2)

                                                                                       ggAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATAc                                            <  2:2091810/150‑1 (MQ=2)

                                                                                       ggAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATAc                                            >  1:3416511/1‑150 (MQ=2)

                                                                                        gAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                           <  2:4456027/150‑1 (MQ=255)

                                                                                        gAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                           <  1:4279452/150‑1 (MQ=255)

                                                                                        gAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                           <  2:2598753/150‑1 (MQ=255)

                                                                                        gAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                           >  2:3165454/1‑150 (MQ=2)

                                                                                         aGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGGTGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑AGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtt                                          >  2:4261521/1‑150 (MQ=2)

                                                                                         aGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtt                                          >  1:1650637/1‑150 (MQ=2)

                                                                                          ggTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                         >  1:844691/1‑150 (MQ=2)

                                                                                           gTCATGCAGCAAGTCTAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                        <  2:1694682/150‑1 (MQ=2)

                                                                                           gTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                        <  1:1530/150‑1 (MQ=2)

                                                                                           gTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                        >  2:743939/1‑150 (MQ=255)

                                                                                           gTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                        <  2:2305787/150‑1 (MQ=2)

                                                                                           gTCATGCAGCAAGTCAAGACAAGGACGTAAAAGATGTCAGTTTACCGTTCATACAGCCCGTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                        <  2:1709647/150‑1 (MQ=255)

                                                                                            tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTg                                       <  2:877348/150‑1 (MQ=255)

                                                                                             cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTga                                      <  1:3489650/150‑1 (MQ=9)

                                                                                             cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTga                                      <  1:2754092/150‑1 (MQ=9)

                                                                                             cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTga                                      <  2:155011/150‑1 (MQ=9)

                                                                                             cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTga                                      <  2:180161/150‑1 (MQ=9)

                                                                                              aTGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                     >  1:2033160/1‑150 (MQ=9)

                                                                                              aTGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGTTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                     >  2:1390922/1‑150 (MQ=9)

                                                                                              aTGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                     >  2:2379401/1‑150 (MQ=9)

                                                                                              aTGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                     >  2:4164997/1‑150 (MQ=9)

                                                                                               tGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaa                                    <  1:4131417/150‑1 (MQ=9)

                                                                                               tGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaa                                    <  2:2674103/150‑1 (MQ=9)

                                                                                                gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaa                                   <  2:3625006/150‑1 (MQ=255)

                                                                                                 cagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaa                                  <  1:2680094/150‑1 (MQ=9)

                                                                                                  agcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaag                                 <  1:1124930/150‑1 (MQ=9)

                                                                                                  agcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaag                                 >  1:947553/1‑150 (MQ=9)

                                                                                                   gcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaaga                                <  1:3356541/150‑1 (MQ=9)

                                                                                                   gcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaaga                                >  1:701127/1‑150 (MQ=255)

                                                                                                    caAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaa                               <  1:1849910/150‑1 (MQ=255)

                                                                                                    caAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaa                               <  1:3453092/150‑1 (MQ=255)

                                                                                                     aaGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaa                              >  1:2322288/1‑150 (MQ=9)

                                                                                                        tCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaaaan                           <  2:947553/150‑2 (MQ=255)

                                                                                                        tCAAGACAAGCAGGTAAAAGATGTCAGTTGACCGTTCATACAGTCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGaaaaaa                           <  1:4399312/150‑1 (MQ=255)

                                                                                                          aaGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGaaaaaaaa                         >  2:513060/1‑150 (MQ=9)

                                                                                                             aCAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaagat                      <  1:2848969/150‑2 (MQ=9)

                                                                                                             aCAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaagat                      >  2:4143427/1‑149 (MQ=9)

                                                                                                              cAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaagatg                     <  1:3767857/150‑3 (MQ=9)

                                                                                                              cAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaagacg                     >  1:4507698/1‑148 (MQ=9)

                                                                                                               aaGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAagatga                    <  1:3094416/150‑1 (MQ=9)

                                                                                                               aaGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAagatga                    >  1:1608970/1‑150 (MQ=255)

                                                                                                               aaGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAagatga                    >  1:1488314/1‑150 (MQ=255)

                                                                                                               aaGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTAATAAG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTAAAAAAAAAAAAAAAagacga                    <  2:2493527/150‑1 (MQ=9)

                                                                                                                 ggaggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgata                  <  1:4006959/150‑1 (MQ=9)

                                                                                                                 ggaggtAAAAGATGTCAGTTGACCGTTAATACAGCCCTTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgata                  <  2:2912571/150‑1 (MQ=9)

                                                                                                                   aggtcaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataag                <  1:3487814/145‑1 (MQ=9)

                                                                                                                   aggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataag                <  2:202463/150‑1 (MQ=12)

                                                                                                                    ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATAGTTTTGAAAAAGAAAAAAAAGatgataaaa               >  1:3540286/4‑148 (MQ=9)

                                                                                                                    ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataaga               <  1:1196615/147‑1 (MQ=255)

                                                                                                                    ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataaga               <  1:2989974/147‑1 (MQ=255)

                                                                                                                    ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTAAAAAAAGAAAAAAAAGatgataaga               <  2:3786129/147‑1 (MQ=255)

                                                                                                                       aaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataagaaaa            >  1:1356010/1‑147 (MQ=255)

                                                                                                                        aaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAAt           >  1:2154653/1‑150 (MQ=9)

                                                                                                                         aaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAAtt          <  2:1232225/150‑1 (MQ=255)

                                                                                                                           gATGTCAGTTGACCGTTCAGACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCa        <  2:3964977/150‑1 (MQ=255)

                                                                                                                             tGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAg      >  1:4442148/1‑150 (MQ=255)

ATTAGAGAAACATTCGATTTTCTATCTATACAAAAAGCAAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTATTAG‑GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATT  >  CP043523/1885651‑1885929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`