BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,885,804	C→T	Y10Y (TAC→TAT)	FTN78_chr1931 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,885,804	0	C	T	99.7%	560.7 / NA	308	Y10Y (TAC→TAT)	FTN78_chr1931	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (141/164); total (141/167)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 308 total aligned reads displayed.

GAAACATTCGATTTTCTATCTATACAAAAAGCAAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTATTAG‑GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATT  >  CP043523/1885657‑1885929

                            aggCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCCTTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAggg                                                                                                 >  2:3747436/3‑149 (MQ=14)

                              gCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGat                                                                                               >  2:3584142/1‑150 (MQ=14)

                                aaGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGatga                                                                                             >  1:1698037/1‑150 (MQ=14)

                                    agtTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGaa                                                                                         >  2:191672/1‑150 (MQ=14)

                                    agtTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGaa                                                                                         >  2:12721/1‑150 (MQ=14)

                                      tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAAt                                                                                       <  1:4114900/150‑1 (MQ=9)

                                          tCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATgggg                                                                                   <  1:1808500/150‑1 (MQ=9)

                                              aggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                               <  2:3988812/145‑1 (MQ=9)

                                              aggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                               >  2:1428216/6‑150 (MQ=14)

                                                 attCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAttt                                                                            <  2:2688023/148‑1 (MQ=14)

                                                 attCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCAttt                                                                            <  1:294148/148‑1 (MQ=14)

                                                  ttCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTg                                                                           >  1:67297/2‑150 (MQ=14)

                                                    cACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCg                                                                         <  2:2604308/150‑1 (MQ=14)

                                                    cACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCg                                                                         >  1:1010248/1‑150 (MQ=14)

                                                     aCGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGa                                                                        >  2:2567853/1‑150 (MQ=14)

                                                     aCGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGa                                                                        <  2:3922191/150‑1 (MQ=14)

                                                       gtgtCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACt                                                                      <  2:1781326/150‑1 (MQ=17)

                                                        tgtCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTg                                                                     <  1:4335212/150‑1 (MQ=14)

                                                         gtCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCGCGATAGAGGGATGATGAAATGGGGCGCATTGGCGACTgg                                                                    <  2:247472/150‑1 (MQ=14)

                                                           cATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTggag                                                                  >  1:4362931/1‑148 (MQ=14)

                                                            aTTTATCGGAACAAACTCATTGGAGGTCATGGAGCAAGTCAAGACAAGGCGGTAAAAGATGTCAGTTGACCGTTCATACTGCCCCTACGAAGTCATCCGTGGTG‑ATCACGATAGAGTGATGATGAAATGGGGCGCATTTCCGACTGgaga                                                                 <  2:4358028/150‑1 (MQ=12)

                                                            aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTggaga                                                                 >  2:1490045/1‑147 (MQ=14)

                                                            aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGgaga                                                                 >  2:3115784/1‑150 (MQ=14)

                                                            aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGgaga                                                                 >  1:2395911/1‑150 (MQ=14)

                                                             tttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaa                                                                <  1:2615835/150‑1 (MQ=14)

                                                              ttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaac                                                               >  1:3572837/1‑149 (MQ=9)

                                                              ttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaac                                                               <  1:815400/150‑2 (MQ=14)

                                                               tATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaact                                                              <  1:201508/150‑3 (MQ=14)

                                                               tATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCGATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaact                                                              <  2:563425/150‑3 (MQ=14)

                                                                  cGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcac                                                           >  1:1508023/1‑145 (MQ=9)

                                                                   ggAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                          >  2:3184626/1‑144 (MQ=9)

                                                                   ggAACAAACGCATTGGAGGTCATGAAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTGTGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                          >  2:3834014/1‑144 (MQ=9)

                                                                    gAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacgg                                                         <  2:1156429/150‑8 (MQ=9)

                                                                    gAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacgg                                                         <  2:3601527/150‑8 (MQ=9)

                                                                    gAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacgg                                                         >  2:3038851/1‑143 (MQ=9)

                                                                              aTTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCaaaa                                               >  2:1002806/1‑150 (MQ=2)

                                                                                tGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATa                                             >  2:3014202/1‑150 (MQ=2)

                                                                                 ggAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATAc                                            <  1:3053288/150‑1 (MQ=2)

                                                                                 ggAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATAc                                            <  1:2982638/150‑1 (MQ=2)

                                                                                 ggAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATAc                                            <  2:2091762/150‑1 (MQ=2)

                                                                                  gAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                           <  1:4287441/150‑1 (MQ=255)

                                                                                  gAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                           <  2:4514907/150‑1 (MQ=255)

                                                                                  gAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                           <  1:1649633/150‑1 (MQ=255)

                                                                                  gAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                           >  2:786089/1‑150 (MQ=2)

                                                                                   aGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtt                                          <  1:4437836/150‑1 (MQ=2)

                                                                                   aGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtt                                          <  1:3986798/150‑1 (MQ=2)

                                                                                   aGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtt                                          <  1:2119949/150‑1 (MQ=2)

                                                                                   aGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtt                                          <  1:2716637/150‑1 (MQ=2)

                                                                                   aGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑GGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtt                                          >  2:4259880/1‑150 (MQ=9)

                                                                                    ggTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                         <  2:1352606/150‑1 (MQ=2)

                                                                                    ggTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                         >  1:844691/1‑150 (MQ=2)

                                                                                     gTCATGCAGCAAGTCTAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                        <  2:1694682/150‑1 (MQ=2)

                                                                                     gTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                        <  1:3433139/150‑1 (MQ=2)

                                                                                     gTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                        <  2:749938/150‑1 (MQ=2)

                                                                                     gTCATGCAGCAAGTCAAGACAAGGACGTAAAAGATGTCAGTTTACCGTTCATACAGCCCGTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                        <  2:1709647/150‑1 (MQ=255)

                                                                                      tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTg                                       <  1:1452070/150‑1 (MQ=255)

                                                                                      tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTg                                       <  2:877348/150‑1 (MQ=255)

                                                                                       cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTga                                      <  1:2754092/150‑1 (MQ=9)

                                                                                       cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTga                                      <  1:3489650/150‑1 (MQ=9)

                                                                                       cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTga                                      <  2:155011/150‑1 (MQ=9)

                                                                                        aTGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                     <  2:723119/150‑1 (MQ=9)

                                                                                        aTGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                     <  2:2342721/150‑1 (MQ=9)

                                                                                         tGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaa                                    <  2:2673607/150‑1 (MQ=9)

                                                                                         tGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaa                                    >  2:1989055/1‑150 (MQ=255)

                                                                                         tGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaa                                    <  1:4130741/150‑1 (MQ=9)

                                                                                          gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaa                                   <  1:511440/150‑1 (MQ=255)

                                                                                            agcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaag                                 <  1:1124930/150‑1 (MQ=9)

                                                                                            agcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaag                                 >  1:1345486/1‑150 (MQ=9)

                                                                                             gcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaaga                                >  1:532697/1‑150 (MQ=255)

                                                                                              caAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaa                               <  1:3454517/150‑1 (MQ=255)

                                                                                              caAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGTACTCACGGAGGCTCAAAATACTTTTgaaaaagaa                               >  1:4033877/1‑150 (MQ=9)

                                                                                              caAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTaaaaaagaa                               <  2:288411/150‑1 (MQ=255)

                                                                                                  tCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaaaan                           <  2:947553/150‑2 (MQ=255)

                                                                                                  tCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGaaaaaa                           >  2:1226298/1‑150 (MQ=12)

                                                                                                   cAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGaaaaaaa                          >  2:2224238/1‑150 (MQ=9)

                                                                                                    aaGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGaaaaaaaa                         <  1:4500904/150‑1 (MQ=12)

                                                                                                    aaGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaacaga                                >  1:156593/1‑143 (MQ=2)

                                                                                                    aaGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGaaaaaaaa                         >  2:512959/1‑150 (MQ=9)

                                                                                                     aGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTAGGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaag                        <  1:4158803/150‑1 (MQ=12)

                                                                                                       aCAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaagat                      >  2:4143427/1‑149 (MQ=9)

                                                                                                       aCAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaagat                      <  1:2848969/150‑2 (MQ=9)

                                                                                                        cAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaagatg                     <  1:3767857/150‑3 (MQ=9)

                                                                                                        cAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaagatg                     >  2:3487981/1‑148 (MQ=9)

                                                                                                        cAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAAAAAAAAaagatg                     <  2:703741/150‑3 (MQ=9)

                                                                                                        cAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTAATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAAAAAAAAaagatg                     <  2:3658246/150‑3 (MQ=9)

                                                                                                         aaGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaagatga                    >  2:825453/1‑147 (MQ=255)

                                                                                                          aggaggTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAgatgat                   >  1:2480151/1‑150 (MQ=9)

                                                                                                           ggaggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgata                  >  1:2687084/1‑150 (MQ=9)

                                                                                                           ggaggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgata                  <  1:2312512/150‑1 (MQ=9)

                                                                                                           ggaggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCACTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGaagcta                  >  1:3197780/1‑150 (MQ=9)

                                                                                                            gaggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataa                 >  1:3040108/1‑150 (MQ=9)

                                                                                                             aggtcaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataag                <  1:3487814/145‑1 (MQ=9)

                                                                                                              ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATAGTTTTGAAAAAGAAAAAAAAGatgataaaa               >  1:3540286/4‑148 (MQ=9)

                                                                                                              ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataaga               <  1:1196615/147‑1 (MQ=255)

                                                                                                              ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataaga               <  1:4513168/147‑1 (MQ=255)

                                                                                                              ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTAAAAAAAGAAAAAAAAGatgataaga               <  2:3786129/147‑1 (MQ=255)

                                                                                                                   aaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAAtt          >  1:4272350/1‑150 (MQ=9)

                                                                                                                   aaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAAtt          <  2:3429287/150‑1 (MQ=255)

                                                                                                                     gATGTCAGTTGACCGTTCAGACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCa        <  2:3964977/150‑1 (MQ=255)

                                                                                                                          cAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGACAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGctt   >  1:3729152/1‑148 (MQ=255)

GAAACATTCGATTTTCTATCTATACAAAAAGCAAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTATTAG‑GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATT  >  CP043523/1885657‑1885929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`