Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,895,466 T→C L196L (TTA→CTA)  FTN78_chr1944 → Mobile element protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,895,4660TC81.1% 2892.9 / inf 2202L196L (TTA→CTA) FTN78_chr1944Mobile element protein
Reads supporting (aligned to +/- strand):  ref base T (177/237);  new base C (799/980);  total (978/1224)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

Only 100 of 2208 total aligned reads displayed.

ATGGATTTAAATGTCATGCTTTAGTCAGTGAATCAGGCTATGTCATAGACTACACAATTACTCCTGCTTCAATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTCTCTTTTTTGACAAAT  >  CP043523/1895320‑1895615
                                                                                                                                                  |                                                                                                                                                     
atggctgtAAATGTCATGCTTTAGTCAGTGAATCAGGCTATGTCATAGACTACACAATTACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAg                                                                                                                                                    >  2:1190040/8‑150 (MQ=0)
    atgtAAATGTCATGCTTTAGTCAGTGAATCAGGCTATGTCATAGACTACACAATTACTCCTGCTTCAATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGgtca                                                                                                                                                >  1:4499166/4‑150 (MQ=1)
                   ttcaGTCAGTGAATCAGGCTATGTCATAGACTACACAATTACTCCTGCTTCAATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAg                                                                                                                                 >  1:4432565/4‑150 (MQ=1)
                                               ggttACACTGTTCCTCGTGGTTGAATGGCTGATCGTGCAATGCTCGAGCAAGTGTTGAGTGCATTTGGGCCGCCAACAGGCCTTGGAGATATGGGATATCTAGGTCGGTCACTGCATGATAGAAAAGAATTAAAAGGAATTGATCTAATg                                                                                                     <  2:2469391/147‑1 (MQ=2)
                                                aCTACACAATTACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGa                                                                                                    <  2:2624426/150‑1 (MQ=14)
                                                aCTACACAATTACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGACAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGa                                                                                                    >  1:3583340/1‑150 (MQ=9)
                                                  tACACAATTACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGaca                                                                                                  >  2:1372239/1‑150 (MQ=12)
                                                   acacAATTACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGacac                                                                                                 <  1:2608675/150‑1 (MQ=12)
                                                    cacaATTACTCCTGCTTCAATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACAcc                                                                                                <  1:1505602/150‑1 (MQ=17)
                                                      caATTACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTg                                                                                              >  2:545169/1‑150 (MQ=12)
                                                       aaTTACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGt                                                                                             <  1:519214/150‑1 (MQ=17)
                                                       aaTTACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGt                                                                                             >  2:2304325/1‑150 (MQ=17)
                                                        aTTACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTc                                                                                            >  1:3190826/1‑150 (MQ=17)
                                                        aTTACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTc                                                                                            <  2:1915524/150‑1 (MQ=12)
                                                        aTTACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTc                                                                                            >  1:3340344/1‑150 (MQ=12)
                                                         ttACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCa                                                                                           <  1:401570/150‑1 (MQ=17)
                                                          tACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAg                                                                                          <  1:2903047/150‑1 (MQ=12)
                                                            cTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGga                                                                                        <  1:850291/150‑1 (MQ=12)
                                                             tCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGgaa                                                                                       <  2:736017/150‑1 (MQ=12)
                                                               cTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGgaaga                                                                                     >  1:207427/1‑150 (MQ=12)
                                                               cTGCTTCAATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGgaaga                                                                                     >  1:3821759/1‑150 (MQ=17)
                                                                 acTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGAAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAAc                                                                                   >  2:3091732/2‑150 (MQ=255)
                                                                  cTTCAATGGCTCATCGTTGAATGACCGACGAAGTGTTGAGTCAATTTGGGACACCACCAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGTAATTGATCTAATGACACCTGTCAGGAAGAACa                                                                                  <  2:4368346/150‑1 (MQ=11)
                                                                    tCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATg                                                                                <  1:3661703/150‑1 (MQ=12)
                                                                    tCAATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATg                                                                                <  2:2106599/150‑1 (MQ=17)
                                                                     cAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAGAGGAATTGATCTAATGACACCTGTCAGGAGGAACATGa                                                                               >  1:3872387/1‑150 (MQ=255)
                                                                      aaTGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGaa                                                                              >  2:2852386/1‑150 (MQ=17)
                                                                      aaTGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGaa                                                                              <  1:3001848/150‑1 (MQ=17)
                                                                       aTGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGaat                                                                             <  1:2001218/150‑2 (MQ=17)
                                                                        tGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAga                                                                            <  1:2511/150‑2 (MQ=12)
                                                                        tGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGc                                                                            <  2:905490/150‑1 (MQ=12)
                                                                         ggCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCa                                                                           >  1:4369439/1‑150 (MQ=12)
                                                                         ggCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCa                                                                           >  2:163541/1‑150 (MQ=12)
                                                                          gCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCaa                                                                          >  1:1265091/1‑150 (MQ=12)
                                                                            tGATAGTTCAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCaaaa                                                                        >  1:3274972/1‑150 (MQ=17)
                                                                             gATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGAGATATCTAGGTCACTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAg                                                                       >  2:4141948/1‑150 (MQ=255)
                                                                             gATAGTTCAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAg                                                                       >  1:1014269/1‑150 (MQ=17)
                                                                              atcgTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGGAAAAGa                                                                      >  2:3825854/4‑150 (MQ=12)
                                                                              atcgTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGa                                                                      >  1:4076581/4‑150 (MQ=17)
                                                                                cgTTCAATGACCGAGGACGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGaaa                                                                    <  1:4204802/149‑1 (MQ=12)
                                                                                cgTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGaaa                                                                    <  2:2953633/149‑1 (MQ=17)
                                                                                  ttCAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAAt                                                                  >  1:1428158/1‑150 (MQ=17)
                                                                                  ttCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAAt                                                                  <  2:4020188/150‑1 (MQ=17)
                                                                                  ttCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAAt                                                                  <  1:2432473/150‑1 (MQ=17)
                                                                                   tCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATc                                                                 >  1:4292894/1‑150 (MQ=17)
                                                                                    cAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATcc                                                                <  2:2021862/150‑1 (MQ=17)
                                                                                    cAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATcc                                                                >  1:2369806/1‑150 (MQ=17)
                                                                                     aaTGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCt                                                               >  2:1267391/1‑150 (MQ=17)
                                                                                      aTGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCtt                                                              <  1:177558/150‑1 (MQ=17)
                                                                                      aTGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCtt                                                              >  1:1633136/1‑150 (MQ=17)
                                                                                      aTGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCtt                                                              <  2:1000360/150‑1 (MQ=17)
                                                                                       tGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCttt                                                             <  2:3176164/150‑1 (MQ=17)
                                                                                        gACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCtttt                                                            >  2:817581/1‑150 (MQ=17)
                                                                                          ccGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTcc                                                          >  2:2534229/1‑150 (MQ=17)
                                                                                             aGGAAGTGTTGAGTAAATTTGGGACACCAACAGTCATTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATGTAATGACAACTGTCAGGAAGAAGATGAAGCAAAAGAAAATCCttttacgta                                                       >  1:1090774/1‑145 (MQ=11)
                                                                                                aaGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAAtt                                                    <  1:4150175/150‑1 (MQ=17)
                                                                                                aaGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAAtt                                                    >  1:4584661/1‑150 (MQ=17)
                                                                                                 aGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAAttt                                                   <  1:2713432/150‑1 (MQ=17)
                                                                                                 aGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAAttt                                                   <  2:1840727/150‑1 (MQ=17)
                                                                                                  tttttGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAAtttt                                                  >  2:603384/4‑150 (MQ=17)
                                                                                                  gtgtTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAAtttt                                                  >  2:4296825/1‑150 (MQ=17)
                                                                                                   tgtTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAAttttt                                                 >  1:771372/1‑150 (MQ=17)
                                                                                                     ttGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCa                                               >  2:32605/1‑150 (MQ=17)
                                                                                                     ttGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCa                                               <  1:1347009/150‑1 (MQ=17)
                                                                                                      tGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCaa                                              <  1:696564/150‑1 (MQ=17)
                                                                                                          tCAATTTGGGACACCAACAGGCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACTCCTGTTAGGAATAACATGAAGCAAAAGAAAATACTTTTCCCTGATTTTTCAATACg                                          >  2:3447597/1‑150 (MQ=11)
                                                                                                             aTTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCaaa                                             <  2:2714766/144‑1 (MQ=17)
                                                                                                             aTTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAg                                       <  2:2784446/150‑1 (MQ=17)
                                                                                                              tttGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGa                                      >  2:2223215/1‑150 (MQ=17)
                                                                                                              tttGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGa                                      <  1:185179/150‑1 (MQ=17)
                                                                                                               ttGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGaa                                     <  1:3934733/150‑1 (MQ=17)
                                                                                                               ttGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGaa                                     <  1:343243/150‑1 (MQ=17)
                                                                                                                tGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGaan                                    <  2:3638613/150‑2 (MQ=17)
                                                                                                                tGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGaaa                                    >  2:4207515/1‑150 (MQ=17)
                                                                                                                tGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGaaa                                    >  1:157834/1‑150 (MQ=17)
                                                                                                                  ggACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAg                                  >  1:2271629/1‑150 (MQ=17)
                                                                                                                  ggACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAg                                  >  1:2861929/1‑150 (MQ=17)
                                                                                                                   gACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGt                                 <  1:3757584/150‑1 (MQ=32)
                                                                                                                   gACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGt                                 <  1:3509139/150‑1 (MQ=17)
                                                                                                                    acacCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTg                                >  1:1922657/1‑150 (MQ=17)
                                                                                                                    acacCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTg                                <  2:3359206/150‑1 (MQ=17)
                                                                                                                     cacCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGa                               >  1:3096773/1‑150 (MQ=17)
                                                                                                                       ccAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGAtt                             <  2:3933952/150‑1 (MQ=17)
                                                                                                                         aaCAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGa                           <  2:3024659/150‑1 (MQ=17)
                                                                                                                            aGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCg                        >  2:466333/1‑150 (MQ=32)
                                                                                                                             gTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGa                       <  1:1187054/150‑1 (MQ=17)
                                                                                                                               ccTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGt                     >  2:3554396/1‑150 (MQ=17)
                                                                                                                               ccTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGt                     >  1:920120/1‑150 (MQ=17)
                                                                                                                                cTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGtt                    <  1:2193942/150‑1 (MQ=17)
                                                                                                                                cTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGtt                    >  2:4519971/1‑150 (MQ=17)
                                                                                                                                 ttGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGttt                   >  2:2394637/1‑150 (MQ=17)
                                                                                                                                 ttGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGttt                   <  2:1560356/150‑1 (MQ=17)
                                                                                                                                  tGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGtttt                  <  2:1786914/150‑1 (MQ=17)
                                                                                                                                      gaTATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAAATAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTctct              >  2:3739279/1‑150 (MQ=17)
                                                                                                                                        tatGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTCTCttt            <  1:168955/150‑1 (MQ=17)
                                                                                                                                          tGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTCTCttttt          >  2:1096641/1‑150 (MQ=17)
                                                                                                                                             gATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTCTCTTTTTTGa       <  1:2780075/150‑1 (MQ=17)
                                                                                                                                              atatTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTCTCTTTTTTGAc      <  2:1476071/150‑1 (MQ=32)
                                                                                                                                              atatCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTCTCTTTTTTGAc      <  1:600013/150‑1 (MQ=17)
                                                                                                                                                  ttAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTCTCTTTTTTGACAAAt  <  2:173813/150‑1 (MQ=32)
                                                                                                                                                  |                                                                                                                                                     
ATGGATTTAAATGTCATGCTTTAGTCAGTGAATCAGGCTATGTCATAGACTACACAATTACTCCTGCTTCAATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTCTCTTTTTTGACAAAT  >  CP043523/1895320‑1895615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑