BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	2,013,099	G→T	A410S (GCT→TCT)	FTN78_chr2083 →	DNA primase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	2,013,099	0	G	T	99.7%	1922.6 / ‑5.5	580	A410S (GCT→TCT)	FTN78_chr2083	DNA primase
Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (292/285); total (294/286)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 582 total aligned reads displayed.

CCAAAGTTTAGAAATAAGTCAAATGGAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCGCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAGTGATTTTCTACCGACCGCCTTT  >  CP043523/2012952‑2013245

  aaaGTTTAGAAATAAGTCAAATGGAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCttt                                                                                                                                                <  2:479769/150‑1 (MQ=255)

  aaaGTTTAGAAATAAGTCAAATGGAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCttt                                                                                                                                                <  2:2118278/150‑1 (MQ=255)

  aaaGTTTAGAAATAAGTCAAATGGAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTAGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCttt                                                                                                                                                <  2:2673327/150‑1 (MQ=255)

   aaGTTTAGAAATAAGTCAAATGGAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGCTAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTa                                                                                                                                               <  2:4247356/150‑1 (MQ=255)

    aGTTTAGAAATAAGTCAAATGGAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTACAATCTCTTTAt                                                                                                                                              <  2:4070445/150‑1 (MQ=255)

    aGTTTAGAAATAAGTCAAATGGAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTAt                                                                                                                                              >  2:619820/1‑150 (MQ=255)

    aGTTTAGAAATAAGTCAAATGGAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTAt                                                                                                                                              <  1:2276118/150‑1 (MQ=255)

          gAAATAAGTCAAATGGAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTa                                                                                                                                        >  2:1454632/1‑150 (MQ=255)

           aaaTAAGTCAAATGGAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTaa                                                                                                                                       >  1:4001188/1‑150 (MQ=255)

            aaTAAGTCAAATGGAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAAc                                                                                                                                      <  2:3094841/150‑1 (MQ=255)

                aGTCAAATGGAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTg                                                                                                                                  <  1:1497739/150‑1 (MQ=255)

                aGTCAAATGGAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTg                                                                                                                                  <  1:1338455/150‑1 (MQ=255)

                    aaaTGGAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGaa                                                                                                                              >  1:686676/1‑150 (MQ=255)

                    aaaTGGAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGaa                                                                                                                              >  2:390278/1‑150 (MQ=255)

                    aaaTGGAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGaa                                                                                                                              <  1:214370/150‑1 (MQ=255)

                         gAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTg                                                                                                                         >  1:2425852/1‑150 (MQ=255)

                         gAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTg                                                                                                                         >  2:2377032/1‑150 (MQ=255)

                           aCATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGAc                                                                                                                       <  1:3506082/150‑1 (MQ=255)

                              tACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGaa                                                                                                                    >  1:4125524/1‑150 (MQ=255)

                              tACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGaa                                                                                                                    <  2:3049113/150‑1 (MQ=255)

                               aCAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAAc                                                                                                                   >  2:2905691/1‑150 (MQ=255)

                               aCAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAAc                                                                                                                   <  2:3752252/150‑1 (MQ=255)

                               aCAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAAc                                                                                                                   <  1:2633833/150‑1 (MQ=255)

                               aCAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGACCGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAACATCTCTTTATCGCTTAACTCTGAGCAGTTTGACGAAc                                                                                                                   >  2:1249044/1‑150 (MQ=255)

                                cAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAAcc                                                                                                                  <  2:2752281/150‑1 (MQ=255)

                                cAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATATCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAAcc                                                                                                                  >  2:1201259/1‑150 (MQ=255)

                                    cGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGaaa                                                                                                              <  2:1596453/150‑1 (MQ=255)

                                        tGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCca                                                                                                          >  1:4055749/1‑150 (MQ=255)

                                        tGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCca                                                                                                          >  2:758149/1‑150 (MQ=255)

                                         gCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCcac                                                                                                         <  1:1635759/150‑1 (MQ=255)

                                          cTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCcaca                                                                                                        >  2:2709485/1‑150 (MQ=255)

                                           ttATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCcacac                                                                                                       <  1:997659/150‑1 (MQ=255)

                                            tATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCcacaca                                                                                                      >  1:498005/1‑150 (MQ=255)

                                             aTCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACaa                                                                                                     >  2:2998191/1‑150 (MQ=255)

                                              tCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAg                                                                                                    <  1:2838005/150‑1 (MQ=255)

                                              tCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAg                                                                                                    >  1:3679281/1‑150 (MQ=255)

                                              tCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCGCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAg                                                                                                    >  2:2183705/1‑150 (MQ=255)

                                               cGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAgg                                                                                                   <  1:849990/150‑1 (MQ=255)

                                               cGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAgg                                                                                                   <  1:586548/150‑1 (MQ=255)

                                                gTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAggg                                                                                                  >  2:864548/1‑150 (MQ=255)

                                                  gCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCt                                                                                                >  2:3608815/1‑150 (MQ=255)

                                                  gCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCt                                                                                                >  2:506685/1‑150 (MQ=255)

                                                   ccTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTg                                                                                               <  1:2924258/150‑1 (MQ=255)

                                                   ccTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTg                                                                                               <  1:2858043/150‑1 (MQ=255)

                                                    cTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGt                                                                                              >  1:2214078/1‑150 (MQ=255)

                                                     ttttGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGtt                                                                                             >  1:3917740/1‑150 (MQ=255)

                                                       ttGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAg                                                                                           >  1:2530647/1‑150 (MQ=255)

                                                        tGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGa                                                                                          >  1:3236379/1‑150 (MQ=255)

                                                                    tACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAAt                                                                              <  2:3484741/150‑1 (MQ=255)

                                                                      ccGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCa                                                                            >  1:2789739/1‑150 (MQ=255)

                                                                       cGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAg                                                                           <  1:4367682/150‑1 (MQ=255)

                                                                       cGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAg                                                                           <  1:4217805/150‑1 (MQ=255)

                                                                          aGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCaa                                                                        >  2:2216431/1‑150 (MQ=255)

                                                                          aGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCaa                                                                        >  1:1915207/1‑150 (MQ=255)

                                                                           gATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAAt                                                                       <  2:440502/150‑1 (MQ=255)

                                                                              aaTGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGAc                                                                    <  1:2043607/150‑1 (MQ=255)

                                                                                tGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACaa                                                                  >  2:2523333/1‑150 (MQ=255)

                                                                                 gACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAAt                                                                 <  1:2671411/150‑1 (MQ=255)

                                                                                       tGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTg                                                           >  2:1087178/1‑150 (MQ=255)

                                                                                        ggAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTgg                                                          >  2:3672501/1‑150 (MQ=255)

                                                                                            aaTAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCttt                                                      <  2:1130677/150‑1 (MQ=255)

                                                                                            aaTAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCttt                                                      >  2:4348380/1‑150 (MQ=255)

                                                                                             aTAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCtttt                                                     >  2:167751/1‑150 (MQ=255)

                                                                                             aTAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCtttt                                                     <  1:3403645/150‑1 (MQ=255)

                                                                                               aaaGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGt                                                   >  2:4026206/1‑150 (MQ=255)

                                                                                                aaGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTa                                                  >  1:3069393/1‑150 (MQ=255)

                                                                                                aaGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTa                                                  >  1:3349745/1‑150 (MQ=255)

                                                                                                    atgatTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATg                                              <  1:2746114/150‑1 (MQ=255)

                                                                                                     tgatTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGa                                             <  1:1970272/150‑1 (MQ=255)

                                                                                                     tgatTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGa                                             >  2:3139589/1‑150 (MQ=255)

                                                                                                      gatTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGat                                            >  1:1188270/1‑150 (MQ=255)

                                                                                                        ttATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGatat                                          <  1:126334/150‑1 (MQ=255)

                                                                                                        ttATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGatat                                          <  1:1007328/150‑1 (MQ=255)

                                                                                                         tataTTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATg                                         >  1:1378/1‑150 (MQ=255)

                                                                                                          atatTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGt                                        <  1:4591553/150‑1 (MQ=255)

                                                                                                             ttAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTg                                     <  2:1383383/150‑1 (MQ=255)

                                                                                                             ttAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTg                                     >  1:3100439/1‑150 (MQ=255)

                                                                                                                  cGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTgaggag                                <  2:2604754/150‑1 (MQ=255)

                                                                                                                  cGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTgaggag                                >  1:1697118/1‑150 (MQ=255)

                                                                                                                  cGCAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTgaggag                                >  1:897971/1‑150 (MQ=255)

                                                                                                                    cAAAGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGtt                              >  1:1813940/1‑150 (MQ=255)

                                                                                                                     aaaGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTc                             <  1:3847863/150‑1 (MQ=255)

                                                                                                                      aaGACGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCg                            <  1:619820/150‑1 (MQ=255)

                                                                                                                         aCGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCa                         <  1:2377032/150‑1 (MQ=255)

                                                                                                                          cGTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAg                        >  2:3380533/1‑150 (MQ=255)

                                                                                                                           gTTTTAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAGt                       >  1:1562608/1‑150 (MQ=255)

                                                                                                                            ttttAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAGTg                      >  1:4445033/1‑150 (MQ=255)

                                                                                                                              ttAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAGTGAt                    <  1:12980/150‑1 (MQ=255)

                                                                                                                               tAGAGTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAGTGAtt                   <  2:3199264/150‑1 (MQ=255)

                                                                                                                                agagTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAGTGAttt                  >  1:2565838/1‑150 (MQ=255)

                                                                                                                                 gagTATGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAGTGAtttt                 >  2:3265197/1‑150 (MQ=255)

                                                                                                                                     aTGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAGTGATTTTCTac             <  2:2789739/150‑1 (MQ=255)

                                                                                                                                     aTGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAGTGATTTTCTac             >  1:2979726/1‑150 (MQ=255)

                                                                                                                                      tGTCCTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAGTGATTTTCTacc            <  1:1104295/150‑1 (MQ=255)

                                                                                                                                          cTTAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAGTGATTTTCTaccgacc        <  2:4559506/150‑1 (MQ=255)

                                                                                                                                            tAAAATCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAGTGATTTTCTACCGACCGc      >  2:1812294/1‑150 (MQ=255)

                                                                                                                                              aaaTCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAGTGATTTTCTACCGACCGCCt    <  2:4166027/150‑1 (MQ=255)

                                                                                                                                              aaaTCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAGTGATTTTCTACCGACCGCCt    <  2:1892023/150‑1 (MQ=255)

                                                                                                                                               aaTCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAGTGATTTTCTACCGACCGCCtt   <  1:1204538/150‑1 (MQ=255)

                                                                                                                                               aaTCTCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAGTGATTTTCTACCGACCGCCtt   <  2:2047184/150‑1 (MQ=255)

CCAAAGTTTAGAAATAAGTCAAATGGAACATACAGACGTTTGCTTATCGTGCCTTTTGAAAAGTCTTTTACCGCAGATAATGACGATTGGAAAATAAAGGATGATTATATTAAACGCAAAGACGTTTTAGAGTATGTCCTTAAAATCGCTTTATCGCTTAACTTTGAGAAGTTTGACGAACCGAAAGCCACACAAGGGCTGTTAGATGACTTTAAAATCAGCAATGACAATGTATTGGCTTTTGTAAATGATATGTTTGAGGAGTTCGTCAGTGATTTTCTACCGACCGCCTTT  >  CP043523/2012952‑2013245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`