Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 2,043,810 C→A D311Y (GAC→TAC)  FTN78_chr2119 ← ATP‑dependent helicase/nuclease AddAB, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435232,043,8100CA99.8% 1602.6 / NA 482D311Y (GAC→TAC) FTN78_chr2119ATP‑dependent helicase/nuclease AddAB, subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (238/242);  minor base G (1/0);  total (240/242)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 482 total aligned reads displayed.

AACTTTGGTAAAACTCAATAACAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTCTTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAGTGGTTATTGCTTCTAAGAGTTCATTTT  >  CP043523/2043663‑2043956
                                                                                                                                                   |                                                                                                                                                  
aaCTTTGGTAAAACTCAATAACAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTAtt                                                                                                                                                  >  2:4491408/1‑150 (MQ=255)
 aCTTTGGTAAAACTCAATAACAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTg                                                                                                                                                 >  1:3997579/1‑150 (MQ=255)
   tttGGTAAAACTCAATAACAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGtt                                                                                                                                               <  2:2188663/150‑1 (MQ=255)
       gTAAAACTCAATAACAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTg                                                                                                                                           >  1:2262158/1‑150 (MQ=255)
         aaaaCTCAATAACAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTa                                                                                                                                         <  1:1938225/150‑1 (MQ=255)
          aaaCTCAATAACAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTAc                                                                                                                                        <  2:3242306/150‑1 (MQ=255)
           aaCTCAATAACAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACt                                                                                                                                       >  2:861179/1‑150 (MQ=255)
             cTCAATAACAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGa                                                                                                                                     >  2:1361066/1‑150 (MQ=255)
              tCAATAACAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGaa                                                                                                                                    <  1:2852902/150‑1 (MQ=255)
                aaTAACAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGaaaa                                                                                                                                  >  1:1040736/1‑150 (MQ=255)
                  tAACAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTTTTTCACTTTACCATGTATTATTTTATCAACAAACTTCCGAATTTCTCTAGGTCTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAatat                                                                                                                                >  2:899302/1‑150 (MQ=255)
                  tAACAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAAt                                                                                                                                >  1:765125/1‑150 (MQ=255)
                  tAACAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAAt                                                                                                                                >  1:2124995/1‑150 (MQ=255)
                   aaCAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATc                                                                                                                               <  1:3673654/150‑1 (MQ=255)
                    aCAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCt                                                                                                                              <  1:1847567/150‑1 (MQ=255)
                     cAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCtt                                                                                                                             >  1:4488537/1‑150 (MQ=255)
                     cAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCtt                                                                                                                             >  1:2432660/1‑150 (MQ=255)
                       aaTTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCtttt                                                                                                                           >  1:2959007/1‑150 (MQ=255)
                       aaTTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCtttt                                                                                                                           <  1:3520670/150‑1 (MQ=255)
                            ttGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCag                                                                                                                      >  2:3069358/1‑150 (MQ=255)
                            ttGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACCAAGTATTGTTTTTGTACTGAAAAATCTTTTTTAag                                                                                                                      >  1:4216454/1‑150 (MQ=255)
                             tGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTTACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAACTCTTTTTTCaga                                                                                                                     >  2:3182194/1‑150 (MQ=255)
                             tGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAATAATCTTTTTTCaga                                                                                                                     <  1:2735854/150‑1 (MQ=255)
                               tAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGc                                                                                                                   <  1:4582723/150‑1 (MQ=255)
                                 aGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCg                                                                                                                 >  2:1452353/1‑150 (MQ=255)
                                     ttCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAg                                                                                                             <  1:1994660/150‑1 (MQ=255)
                                     ttCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAg                                                                                                             <  1:173884/150‑1 (MQ=255)
                                      tCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTTTTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCTGAAAGt                                                                                                            >  2:1568778/1‑150 (MQ=255)
                                       cTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGACTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTa                                                                                                           >  2:4415853/1‑150 (MQ=255)
                                         gCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTAtt                                                                                                         >  2:2562859/1‑150 (MQ=255)
                                         gCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTAtt                                                                                                         <  1:924188/150‑1 (MQ=255)
                                          cGATTTCAAATGCTTGATTTTGCTATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTAttt                                                                                                        <  1:3426698/150‑1 (MQ=255)
                                            aTTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCa                                                                                                      <  2:2132386/150‑1 (MQ=255)
                                             tttCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCAt                                                                                                     >  1:1098221/1‑150 (MQ=255)
                                             tttCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCAt                                                                                                     <  1:3922240/150‑1 (MQ=255)
                                               tCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCt                                                                                                   <  2:3119997/150‑1 (MQ=255)
                                                  aaTGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCtttt                                                                                                >  2:3368716/1‑150 (MQ=255)
                                                   aTGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTg                                                                                               >  1:3658866/1‑150 (MQ=255)
                                                     gCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCtttt                                                                                                <  2:1910409/147‑1 (MQ=255)
                                                     gCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTgaa                                                                                             >  2:4306923/1‑150 (MQ=255)
                                                      cTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTgaag                                                                                            <  2:652873/150‑1 (MQ=255)
                                                         gATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTAATGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAAc                                                                                         <  2:1038629/150‑1 (MQ=255)
                                                          aTTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAAcc                                                                                        >  1:1602682/1‑150 (MQ=255)
                                                           ttttGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAAccc                                                                                       >  1:3335527/1‑150 (MQ=255)
                                                           ttttGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGAATTTCGAGCCCACTAAGTATTGTTTTTGTGCTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAAccc                                                                                       >  2:4468778/1‑150 (MQ=255)
                                                             ttGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCAc                                                                                     >  1:4320042/1‑150 (MQ=255)
                                                               gATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTc                                                                                   >  1:2575456/1‑150 (MQ=255)
                                                               gATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTc                                                                                   <  2:73084/150‑1 (MQ=255)
                                                               gATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTc                                                                                   <  1:858272/150‑1 (MQ=255)
                                                                  atatttCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGaa                                                                                <  2:165436/147‑1 (MQ=255)
                                                                  atTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGaa                                                                                >  1:716839/1‑150 (MQ=255)
                                                                         aatcaaCTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGt                                                                         <  1:1967825/150‑1 (MQ=255)
                                                                            caaCTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATc                                                                      <  1:499648/150‑1 (MQ=255)
                                                                              aCTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTa                                                                    <  2:3471815/150‑1 (MQ=255)
                                                                               cttaCCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGTATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTAt                                                                   <  2:1762560/147‑1 (MQ=255)
                                                                               cTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTAt                                                                   <  1:476286/150‑1 (MQ=255)
                                                                               cTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTAt                                                                   >  2:3810161/1‑150 (MQ=255)
                                                                                   ccATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGAt                                                               >  1:2761193/1‑150 (MQ=255)
                                                                                    cATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATa                                                              >  1:1400145/1‑150 (MQ=255)
                                                                                     aTGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATaa                                                             >  2:3013394/1‑150 (MQ=255)
                                                                                        ttttattttaTCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATaaaaa                                                          >  1:1205674/1‑150 (MQ=255)
                                                                                        ttttattttaTCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATaaaaa                                                          <  1:1534337/150‑1 (MQ=255)
                                                                                           tattttatCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAg                                                       <  2:2644748/150‑1 (MQ=255)
                                                                                              tttatCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTc                                                    >  2:4565796/1‑150 (MQ=255)
                                                                                                tatCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCtg                                                  >  1:811739/1‑150 (MQ=255)
                                                                                                 atCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCtgt                                                 <  2:2006348/150‑1 (MQ=255)
                                                                                                 atCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCtgt                                                 <  2:2683428/150‑1 (MQ=255)
                                                                                                     acaaACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGaan                                             <  2:3619867/150‑2 (MQ=255)
                                                                                                     acaaACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGaaa                                             <  1:3603722/150‑1 (MQ=255)
                                                                                                       aaaCTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGGTAAAAATAGTTCGGTGAAAGt                                           >  1:3847687/1‑150 (MQ=255)
                                                                                                       aaaCTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGt                                           >  1:2366518/1‑150 (MQ=255)
                                                                                                        aaCTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGtn                                          <  2:2872858/150‑2 (MQ=255)
                                                                                                         aCTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTg                                         <  1:594680/150‑1 (MQ=255)
                                                                                                         aCTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTg                                         >  2:2099038/1‑150 (MQ=255)
                                                                                                          cTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGc                                        >  1:4397647/1‑150 (MQ=255)
                                                                                                             ccGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCaaa                                     <  2:2431806/150‑1 (MQ=255)
                                                                                                              cGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCaaaa                                    >  2:2454127/1‑150 (MQ=255)
                                                                                                                aaTTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATc                                  >  1:4516196/1‑150 (MQ=255)
                                                                                                                aaTTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTAGTACTGAAAAATCGTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTGTCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATc                                  >  2:3869998/1‑150 (MQ=255)
                                                                                                                  tttCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCtt                                >  1:1464970/1‑150 (MQ=255)
                                                                                                                  tttCTCCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCtt                                <  2:1242440/150‑1 (MQ=255)
                                                                                                                     ctcCAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAg                             >  2:2738071/1‑150 (MQ=255)
                                                                                                                       ccAGGTTTGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAGTg                           >  1:4155446/1‑150 (MQ=255)
                                                                                                                       ccAGGTTTGGATTTCAAACTCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTAAAAAATAAAAAAAACAGTTCTGTGAAAGTTGCAAAATCTTTAGTg                           <  2:2262158/150‑1 (MQ=255)
                                                                                                                             ttGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGGAAGTTGCAAAATCTTTAGTGGTTAtt                     <  1:2185335/150‑1 (MQ=255)
                                                                                                                              tGGATTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAGTGGTTATTg                    <  1:3022573/150‑1 (MQ=255)
                                                                                                                                 aTTTCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAGTGGTTATTGCtt                 <  1:3276061/150‑1 (MQ=255)
                                                                                                                                  tttCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAGTGGTTATTGCTTc                <  2:2959007/150‑1 (MQ=255)
                                                                                                                                   ttCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAGTGGTTATTGCTTCt               <  2:1067696/150‑1 (MQ=255)
                                                                                                                                   ttCGAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAGTGGTTATTGCTTCt               <  1:3127561/150‑1 (MQ=255)
                                                                                                                                      gAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAGTGGTTATTGCTTCTAag            >  2:4022169/1‑150 (MQ=255)
                                                                                                                                      gAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAGTGGTTATTGCTTCTAag            <  2:3952179/150‑1 (MQ=255)
                                                                                                                                      gAGCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAGTGGTTATTGCTTCTAag            <  2:181107/150‑1 (MQ=255)
                                                                                                                                        gCCCACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGATCCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAATGGTTATTGCTTCTAagag          <  2:4132121/150‑1 (MQ=255)
                                                                                                                                         cccACTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAGTGGTTATTGCTTCTAAGAGt         >  1:1793426/1‑150 (MQ=255)
                                                                                                                                            aCTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAGTGGTTATTGCTTCTAAGAGTTCa      <  2:1256472/150‑1 (MQ=255)
                                                                                                                                            aCTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAGTGGTTATTGCTTCTAAGAGTTCa      <  1:3069358/150‑1 (MQ=255)
                                                                                                                                             cTAAGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAGTGGTTATTGCTTCTAAGAGTTCAt     <  2:494506/150‑1 (MQ=255)
                                                                                                                                               aaGTATTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAGTGGATATTGCTTCGAAGAGTTCAttt   >  1:3397520/1‑150 (MQ=255)
                                                                                                                                               aaGTATTGTTTTTGTACTGAAAAATCATTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAGTGGTTATTGCTTCTAAGAGTTCAttt   <  1:3876499/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACTTTGGTAAAACTCAATAACAAATTTTTGTAAGTTTTCTGCGATTTCAAATGCTTGATTTTGATATTTTTCAATCAACTGACCATGTTTTATTTTATCAACAAACTTCCGAATTTCTCCAGGTTTGGATTTCGAGCCCACTAAGTCTTGTTTTTGTACTGAAAAATCTTTTTTCAGAGCCGAAAGTATTTCATCTTTTGAAGAACCCACTCGAATATCTGTATCTATTGATAAAAATAGTTCTGTGAAAGTTGCAAAATCTTTAGTGGTTATTGCTTCTAAGAGTTCATTTT  >  CP043523/2043663‑2043956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑