Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,860,921 T→C D25D (GAT→GAC)  FTN78_chr1908 → neutral endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,860,9210TC99.6% 758.1 / NA 225D25D (GAT→GAC) FTN78_chr1908neutral endopeptidase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (107/116);  total (109/116)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 225 total aligned reads displayed.

TTTTCGCAATTCTAAAGTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGATAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTGGCTAAAGATTTAGCTGA  >  CP043523/1860775‑1860992
                                                                                                                                                  |                                                                       
ttttCGCAATTCTAAAGTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACaaa                                                                      >  2:764174/1‑150 (MQ=255)
 tttCGCAATTCTAAAGTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAAc                                                                     >  2:3469153/1‑150 (MQ=255)
       aaTTCTAAAGTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGaa                                                               <  1:3074325/150‑1 (MQ=255)
       aaTTCTAAAGTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGaa                                                               >  2:2099501/1‑150 (MQ=255)
       aaTTCTAAAGTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGaa                                                               <  2:801309/150‑1 (MQ=255)
        aTTCTAAAGTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAAt                                                              <  2:3992595/150‑1 (MQ=255)
        aTTCTAAAGTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAAt                                                              <  2:1535411/150‑1 (MQ=255)
        aTTCTAAAGTCACAGAAGGTCAAATTTATGATTTGAGGTTCTTTTATTAAAAAAGGAGTGTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCGGCTGACAAACCAAGAAt                                                              <  2:693243/150‑1 (MQ=21)
            tAAAGTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTcn                                                          <  2:2873282/150‑2 (MQ=255)
            tAAAGTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCa                                                          <  1:4528507/150‑1 (MQ=255)
             aaaGTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAg                                                         >  2:934023/1‑150 (MQ=255)
             aaaGTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAg                                                         >  2:3390570/1‑150 (MQ=255)
             aaaGTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAg                                                         >  1:4385285/1‑150 (MQ=255)
              aaGTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGc                                                        <  1:2546691/150‑1 (MQ=255)
               aGTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCt                                                       >  2:1229368/1‑150 (MQ=255)
               aGTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCt                                                       <  2:829832/150‑1 (MQ=255)
                gTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCtt                                                      >  1:542308/1‑150 (MQ=255)
                gTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCtt                                                      >  2:1844917/1‑150 (MQ=255)
                gTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCtt                                                      <  1:342487/150‑1 (MQ=255)
                 tCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCttt                                                     <  1:3362938/150‑1 (MQ=255)
                  cacaGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGCAATTCCTGCTGACAAACCAAGAATTTCAGCtttt                                                    >  1:4020961/1‑150 (MQ=255)
                  cacaGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCtttt                                                    >  2:3983233/1‑150 (MQ=255)
                  cacaGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCtttt                                                    >  1:614683/1‑150 (MQ=255)
                  cacaGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCtttt                                                    >  2:906023/1‑150 (MQ=255)
                    caGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTtga                                                  >  1:3038396/1‑150 (MQ=255)
                    caGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTtga                                                  <  2:1559269/150‑1 (MQ=255)
                     aGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTtgat                                                 <  1:1603157/150‑1 (MQ=255)
                     aGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTtgat                                                 <  1:3687588/150‑1 (MQ=255)
                     aGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTAGCTACTGTTAAAGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTtgat                                                 <  2:2915024/150‑1 (MQ=255)
                      gAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTtgatg                                                >  1:957617/1‑150 (MQ=255)
                       aaGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTtgatga                                               <  2:3969117/150‑1 (MQ=255)
                       aaGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTtgatga                                               >  1:2693411/1‑150 (MQ=255)
                       aaGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTtgatga                                               <  2:2234086/150‑1 (MQ=255)
                       aaGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTtgatga                                               >  1:321374/1‑150 (MQ=255)
                          ttCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAAtt                                            >  1:2956090/1‑150 (MQ=255)
                           tCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTa                                           >  1:4588077/1‑150 (MQ=255)
                           tCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTa                                           <  1:140793/150‑1 (MQ=255)
                            cAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAg                                          >  2:2710102/1‑150 (MQ=255)
                            cAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAg                                          >  1:4467389/1‑150 (MQ=255)
                            cAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAg                                          <  1:147503/150‑1 (MQ=255)
                             aaaTTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGt                                         <  1:2255719/150‑1 (MQ=255)
                               aTTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTAc                                       >  2:1276746/1‑150 (MQ=255)
                               aTTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTAc                                       <  2:4273987/150‑1 (MQ=255)
                                 ttATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTa                                     <  1:2911707/150‑1 (MQ=255)
                                 ttATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTa                                     >  1:3638012/1‑150 (MQ=255)
                                 ttATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTa                                     >  1:3638468/1‑150 (MQ=255)
                                   aTGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTaaa                                   <  2:1186302/150‑1 (MQ=255)
                                     gATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTaaaaa                                 <  2:571346/150‑1 (MQ=255)
                                     gATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTaaaaa                                 >  2:2808862/1‑150 (MQ=255)
                                     gATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTaaaaa                                 >  2:1805877/1‑150 (MQ=255)
                                     gATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTaaaaa                                 >  2:1805395/1‑150 (MQ=255)
                                      aTTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTaaaaaa                                >  1:1182443/1‑150 (MQ=255)
                                          gAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATgag                            >  1:2388349/1‑150 (MQ=255)
                                          gAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATgag                            <  1:1026058/150‑1 (MQ=255)
                                          gAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATgag                            >  2:653964/1‑150 (MQ=255)
                                           aGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGATAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATgaga                           <  2:4035410/150‑1 (MQ=255)
                                           aGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATgaga                           >  2:1006197/1‑150 (MQ=255)
                                           aGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATgaga                           <  1:3272263/150‑1 (MQ=255)
                                           aGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATgaga                           <  1:2109326/150‑1 (MQ=255)
                                            gCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGaa                          >  2:1722672/1‑150 (MQ=255)
                                            gCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGaa                          <  2:313633/150‑1 (MQ=255)
                                             cttcttTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACAAAAAAATGAGaaa                         <  2:753920/150‑1 (MQ=255)
                                              ttcttTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGaaaa                        <  1:2007569/150‑1 (MQ=255)
                                               tcttTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGaaaaa                       >  1:2211339/1‑150 (MQ=255)
                                                cttTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAAt                      <  2:4607191/150‑1 (MQ=255)
                                                cttTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAAt                      <  2:4016056/150‑1 (MQ=255)
                                                cttTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAAt                      >  1:3540949/1‑150 (MQ=255)
                                                cttTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAAt                      >  1:1954603/1‑150 (MQ=255)
                                                cttTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAAt                      >  2:1380403/1‑150 (MQ=255)
                                                 ttttATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAAtt                     <  2:3658893/150‑1 (MQ=255)
                                                  tttATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAAttt                    <  2:1076571/150‑1 (MQ=255)
                                                  tttATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAAttt                    >  2:1157239/1‑150 (MQ=255)
                                                  tttATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAAttt                    >  1:2149653/1‑150 (MQ=255)
                                                  tttATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAAttt                    <  1:2759943/150‑1 (MQ=255)
                                                  tttATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAAttt                    <  1:3765286/150‑1 (MQ=255)
                                                  actataaATAAAGGAGTTTGATCTGATAAGGATTCAAGATGAATTATTCGCTACTGTTAATGCTGAATGGTTTGAAAATGCAGAAATTCCTGCTTACAAACTAAGAATTTCAGCTTTTGATGAATGAGTACTAAAAAATGAGAAACAttt                    <  2:1947481/148‑1 (MQ=11)
                                                   ttattaAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAAtttn                   <  2:1501495/150‑2 (MQ=255)
                                                   ttattaAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTg                   <  1:3981204/150‑1 (MQ=255)
                                                   ttattaAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTg                   <  2:4120672/150‑1 (MQ=255)
                                                   ttattaAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTg                   <  2:261971/150‑1 (MQ=255)
                                                    tattaAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTgg                  >  1:415919/1‑150 (MQ=255)
                                                    tattaAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTgg                  >  2:2285553/1‑150 (MQ=255)
                                                     attaTAAAAGGAGTTTGCTATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTATAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGCACTAAAAAATGAGAAAAATTTGGc                 <  2:3859213/150‑1 (MQ=11)
                                                      ttaAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTGGCt                <  2:2505097/150‑1 (MQ=255)
                                                      ttaAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTCTTGATGAATTAGTACTAAAAAATGAGAAAAATTTGGCt                >  2:3344339/1‑150 (MQ=255)
                                                         aaaaaGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTGGCTaaa             >  1:920152/1‑150 (MQ=255)
                                                         aaaaaGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTGGCTaaa             <  1:1266838/150‑1 (MQ=38)
                                                          aaaaGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTGGCTAAAg            <  2:2136168/150‑1 (MQ=25)
                                                          aaaaGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTGGCTAAAg            <  2:852019/150‑1 (MQ=255)
                                                            aaGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTGGCTAAAGAt          >  2:3907097/1‑150 (MQ=37)
                                                             aGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTGGCTAAAGAtt         <  2:4572496/150‑1 (MQ=25)
                                                              ggAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTGGCTAAAGAttt        <  1:1223719/150‑1 (MQ=25)
                                                               gAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTGCTGCTGGCAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAGAAATGAGAAAAATTTGGCTAAAGATTTa       >  2:4334049/1‑150 (MQ=255)
                                                               gAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTGGCTAAAGATTTa       <  2:2666792/150‑1 (MQ=21)
                                                               gAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTGGCTAAAGATTTa       >  1:31567/1‑150 (MQ=21)
                                                               gAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTGGCTAAAGATTTa       >  2:1969319/1‑150 (MQ=21)
                                                                aGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAGAAATTTGGCTAAAGATTTAg      <  1:232948/150‑1 (MQ=18)
                                                                  tttGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTGGCTAAAGATTTAGCt    <  2:2979686/150‑1 (MQ=17)
                                                                  tttGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTGGCTAAAGATTTAGCt    <  1:1844917/150‑1 (MQ=17)
                                                                   ttGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGACAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTGGCTAAAGATTTAGCTg   <  1:619082/150‑1 (MQ=14)
                                                                                                                                                  |                                                                       
TTTTCGCAATTCTAAAGTCACAGAAGTTCAAATTTATGATTTGAGCTTCTTTTATTAAAAAAGGAGTTTGATATGACAAGGATTCAAGATGATTTATTCGCTACTGTTAATGCTGAATGGTTAGAAAATGCAGAAATTCCTGCTGATAAACCAAGAATTTCAGCTTTTGATGAATTAGTACTAAAAAATGAGAAAAATTTGGCTAAAGATTTAGCTGA  >  CP043523/1860775‑1860992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑