Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,861,176 A→G A110A (GCA→GCG)  FTN78_chr1908 → neutral endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,861,1760AG99.2% 1283.8 / NA 375A110A (GCA→GCG) FTN78_chr1908neutral endopeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base G (5/365);  minor base T (0/3);  total (5/370)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 375 total aligned reads displayed.

AAAATAATTTGACTGGTTCTGTTGCAAAGTTTGAAAATCAAACGCACCCGCTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCA  >  CP043523/1861151‑1861322
                         |                                                                                                                                                  
ataaatctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTta                        <  1:3121024/143‑1 (MQ=255)
ataaatctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTta                        <  1:1765175/143‑1 (MQ=255)
ataaatctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTta                        <  2:2967751/143‑1 (MQ=255)
ataaatctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTACCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTta                        <  1:3863467/143‑1 (MQ=255)
 taaatctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTtat                       <  2:3442707/144‑1 (MQ=255)
  aaatctttGACTGTTTCTGTTGCGAAGCTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTtata                      <  1:4079566/145‑1 (MQ=255)
   aatctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTtatat                     <  1:510266/146‑1 (MQ=255)
   aatctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTtatat                     <  2:4596290/146‑1 (MQ=255)
    atctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTtatata                    <  1:317305/147‑1 (MQ=255)
     tctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTatatat                   <  1:322084/148‑1 (MQ=255)
     tctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTatatat                   <  1:2394263/148‑1 (MQ=255)
     tctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTatatat                   <  1:2061412/148‑1 (MQ=255)
     tctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTatatat                   <  2:1212972/148‑1 (MQ=255)
      ctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATAtt                  <  1:4572004/149‑1 (MQ=255)
      ccctatttGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATAtt                  <  1:740601/143‑1 (MQ=255)
       tagaaacggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTc                 <  2:1975287/143‑1 (MQ=255)
       tacaaacggTCCTGTTTCGAAGTTTGAAAATCAAGCGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTACTTCCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTc                 <  1:3707920/143‑1 (MQ=255)
       cctatttGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTc                 <  2:2871263/144‑1 (MQ=255)
       cattgatGGTTCTATTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTc                 <  2:745491/144‑1 (MQ=39)
       atgtaagggTTCTGTAGCGAAGTTTGAAACTCAAACGCGCCCTTTCTGAGCTCAAAATATCTTAGGCTGGTATTACCATTAATATCTTGATTTTAGTGGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATAATc                 <  2:2093703/143‑1 (MQ=25)
       aattgatGGTTCTATTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTc                 <  1:1001819/144‑1 (MQ=38)
        tatttcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTcc                <  1:451081/144‑1 (MQ=255)
        tatttcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTcc                <  1:1326625/144‑1 (MQ=255)
        ctatttGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTcc                <  1:865273/145‑1 (MQ=255)
        attggcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTcc                <  2:95857/144‑1 (MQ=255)
         ttggcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCt               <  1:29642/145‑1 (MQ=39)
         tatttGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCt               <  2:479252/146‑1 (MQ=39)
         tGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTccn               <  2:22583/150‑2 (MQ=255)
         tGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCt               <  2:2910326/150‑1 (MQ=255)
         gaaacggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCt               <  1:831717/145‑1 (MQ=255)
          tttcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc              <  2:1143410/146‑1 (MQ=39)
          tggtGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTc              <  1:647694/147‑1 (MQ=38)
          tggcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc              <  1:1091005/146‑1 (MQ=39)
          tggcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc              <  2:857896/146‑1 (MQ=39)
          taagggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc              <  2:1302275/146‑1 (MQ=39)
          taagggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc              <  1:3425745/146‑1 (MQ=39)
          gcacgggtCTGTTGCGAAGTTTGAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc              <  2:3928797/143‑1 (MQ=38)
          gACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCtn              <  2:1210091/150‑2 (MQ=255)
          gACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc              <  2:1553841/150‑1 (MQ=255)
          gACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc              <  1:2186138/150‑1 (MQ=255)
          gACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc              <  1:2593044/150‑1 (MQ=255)
          aaacggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc              <  2:4110278/146‑1 (MQ=38)
          aaacggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc              <  1:3307461/146‑1 (MQ=255)
           ttcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg             <  1:1712340/147‑1 (MQ=39)
           ggcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg             <  2:1136289/147‑1 (MQ=39)
           aagggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg             <  2:2207936/147‑1 (MQ=39)
           aCTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg             <  1:4313086/150‑1 (MQ=255)
           aCTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg             <  2:383015/150‑1 (MQ=38)
           aCTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg             <  2:2639494/150‑1 (MQ=255)
            gcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGa            <  1:3312921/148‑1 (MQ=39)
            ctgtttCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGa            <  1:3918721/146‑1 (MQ=38)
            atGGTTCTATTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGa            <  2:138701/149‑1 (MQ=39)
             tGGTTCTATTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaa           <  1:2463467/150‑1 (MQ=38)
              gtttCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaat          <  1:469024/148‑2 (MQ=38)
              gtttCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaat          <  2:1761659/148‑2 (MQ=38)
              gggtCTGTTGCGAAGTTTGAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAg          <  1:192900/147‑1 (MQ=38)
              ggTTCTGTTGGGAAGTTTGAAAATCAAGCGCGCCCTCTCTGAACTTCAAATATCTTAGGTTGTTATTCCCATTAGTACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGaat          <  2:3308611/150‑2 (MQ=37)
              ggTTCTGTTGCGAAGTTTGAAACTCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCAATAATACCTTTATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAg          <  2:4218754/150‑1 (MQ=38)
              ggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAg          <  1:3085512/150‑1 (MQ=39)
               tttCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaatg         <  2:2159584/149‑3 (MQ=38)
               tttCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaatg         <  1:155465/149‑3 (MQ=38)
               tttCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaatg         <  1:159252/149‑3 (MQ=38)
               tttCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaatg         <  2:3598204/149‑3 (MQ=38)
               tttCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaatg         <  1:4382535/149‑3 (MQ=38)
               tttCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaatg         <  2:57976/149‑3 (MQ=38)
               ggtCTGTTGCGAAGTTTGAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTCGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAgg         <  2:4531188/148‑1 (MQ=37)
               gTTCTGTTGCGCAGTTTGCAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTACGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaatg         <  2:4393714/150‑3 (MQ=38)
               gTTCTGTTGCGACGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAgg         <  2:3535858/150‑1 (MQ=38)
               gTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGaatt         <  1:1973439/150‑3 (MQ=38)
               gTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaatg         <  1:75907/150‑3 (MQ=255)
               gTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAgg         <  2:4332897/150‑1 (MQ=39)
               gTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAgg         <  1:2002966/150‑1 (MQ=38)
                ttCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAtgg        <  2:3128939/150‑1 (MQ=38)
                ttCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAtgg        <  1:3772192/150‑1 (MQ=38)
                ttCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAtgg        <  2:4422042/150‑1 (MQ=38)
                ttCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAggg        <  1:1499305/150‑1 (MQ=39)
                ttCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCCGAGGCTGGTATTCCCATTAATACCTTGACTACAGAAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAggg        <  1:360586/150‑1 (MQ=37)
                ttCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGaattg        <  2:624334/150‑4 (MQ=38)
                ttCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCGTTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGGGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCGCGAAggg        <  2:3245521/150‑1 (MQ=255)
                 tCTTTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAtggn       <  2:1400/150‑2 (MQ=38)
                 tCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGt       <  1:3053325/150‑1 (MQ=38)
                  cTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtc      <  2:703352/150‑1 (MQ=39)
                  cTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtc      <  1:1857473/150‑1 (MQ=39)
                  cTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtc      <  2:3172601/150‑1 (MQ=38)
                  cTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtc      <  2:1892725/150‑1 (MQ=38)
                   tGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGtct     <  1:2358877/150‑1 (MQ=38)
                   tGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtct     <  1:2283839/150‑1 (MQ=39)
                   tGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGaattgttt     <  2:3079743/150‑7 (MQ=38)
                   tGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaatgatct     <  1:1401682/150‑7 (MQ=38)
                    gTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGtctc    <  1:2579817/150‑1 (MQ=38)
                    gTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtctc    <  2:3404924/150‑1 (MQ=255)
                    gTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGAtctc    <  1:2625807/150‑1 (MQ=38)
                    gTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtctc    <  1:2861623/150‑1 (MQ=39)
                    gTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtctc    <  1:1180297/150‑1 (MQ=39)
                    gTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGAAGACAAAAAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGAtctc    <  2:1005070/150‑1 (MQ=38)
                    attGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtctc    <  1:4598870/149‑1 (MQ=39)
                     ttGCGAAGTTTGAAAATTAACCGCGCCCTCTCTGACCTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTcc   <  1:4198986/150‑1 (MQ=38)
                     ttGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTcc   <  2:2771411/150‑1 (MQ=38)
                     ttGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTcc   <  1:3027562/150‑1 (MQ=38)
                     ttGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTcc   <  1:2774315/150‑1 (MQ=255)
                         |                                                                                                                                                  
AAAATAATTTGACTGGTTCTGTTGCAAAGTTTGAAAATCAAACGCACCCGCTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCA  >  CP043523/1861151‑1861322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑