Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,861,196 A→G H117R (CAC→CGC)  FTN78_chr1908 → neutral endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,861,1960AG99.9% 2218.3 / NA 653H117R (CAC→CGC) FTN78_chr1908neutral endopeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (7/644);  total (7/646)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 656 total aligned reads displayed.

AAAATAATTTGACTGGTTCTGTTGCAAAGTTTGAAAATCAAACGCACCCGCTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAG  >  CP043523/1861151‑1861342
                                             |                                                                                                                                                  
ataaatctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTta                                            <  1:3121024/143‑1 (MQ=255)
ataaatctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTACCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTta                                            <  1:3863467/143‑1 (MQ=255)
  aaatctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTtata                                          <  1:2342448/145‑1 (MQ=255)
  aaatctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTtata                                          <  2:2138361/145‑1 (MQ=255)
     tctttGACTGTTTCTGTTGCTAAGTTTGAAAATTAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCTATTAATACCTTGATTTCAGTAGTTATCGAAAAGCCGAAGAGTGTTCCATTTCTTCGGTTCTTTTTatatat                                       <  2:1877474/148‑1 (MQ=255)
     tctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTtatatan                                       <  2:1210576/148‑2 (MQ=255)
      gagatggtGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATAtt                                      <  1:4600849/143‑1 (MQ=38)
      gagatggtGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATAtt                                      <  1:330763/143‑1 (MQ=38)
      ctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATAtt                                      <  1:2245647/149‑1 (MQ=38)
      ctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATAtt                                      <  2:3376660/149‑1 (MQ=255)
      ctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATAtt                                      <  1:4572004/149‑1 (MQ=255)
       tttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTc                                     <  1:1173263/150‑1 (MQ=255)
       aattgatGGTTCTATTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTc                                     <  1:1001819/144‑1 (MQ=38)
        gatggtGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTcc                                    <  1:1544401/145‑1 (MQ=38)
        attggcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTTCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTcc                                    <  2:4232750/144‑1 (MQ=255)
        attggcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTcc                                    <  2:95857/144‑1 (MQ=255)
         ttgatGGTTCTATTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCt                                   <  2:3424292/146‑1 (MQ=38)
         tatttGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCt                                   <  2:479252/146‑1 (MQ=39)
         tGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCt                                   <  1:617780/150‑1 (MQ=255)
         tGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCt                                   <  1:2684980/150‑1 (MQ=255)
         gaaacggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCt                                   <  1:2407907/145‑1 (MQ=255)
         atttcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCt                                   <  1:3179479/145‑1 (MQ=39)
          tttcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc                                  <  2:1143410/146‑1 (MQ=39)
          tggtGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTc                                  <  1:4405297/147‑1 (MQ=38)
          taagggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc                                  <  2:1302275/146‑1 (MQ=39)
          gcacgggtCTGTTGCGAAGTTTGAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc                                  <  2:2342544/143‑1 (MQ=38)
          gACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc                                  <  2:1553841/150‑1 (MQ=255)
           ttcggTTCTGTTTCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg                                 <  1:3384625/147‑1 (MQ=39)
           aagggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg                                 <  2:2207936/147‑1 (MQ=39)
           aacggTTCTGTTGCGAAGTTTGAATATCAGACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg                                 <  2:4538486/147‑1 (MQ=255)
           aCTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg                                 <  2:3117119/150‑1 (MQ=255)
             tgtttCTATTTCTAATTTTTAAAATCAAACTTTCCCTCTCTGAACTCCAAATATCTTATTCTGTTATTCCCATTAATACCTTGATTTCAGTATACATCTAAAATCCGAATATCGTTCCATTTCTTCGTTTCTTTTTATATATTCCTCGaa                               <  2:66198/147‑1 (MQ=255)
             tGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaa                               <  2:2990635/150‑1 (MQ=255)
              gtttCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaat                              <  1:1585449/148‑2 (MQ=38)
              gggtCTGTTGCGAAGTTTGAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAg                              <  1:192900/147‑1 (MQ=38)
               tttCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaatg                             <  1:3078795/149‑3 (MQ=38)
               tttCTGTTGCGAAGTTTCAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCACTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaatn                             <  2:3881527/149‑3 (MQ=37)
               gTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGaatt                             <  1:1973439/150‑3 (MQ=38)
               gTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGaatt                             <  1:3049013/150‑3 (MQ=38)
               gTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaatg                             <  1:75907/150‑3 (MQ=255)
               gTTCTATTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAgg                             <  2:580318/150‑1 (MQ=38)
                ttCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGAAACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAggg                            <  2:4154722/150‑1 (MQ=39)
                ttCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGaattg                            <  1:4197751/150‑4 (MQ=37)
                ttCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGaattg                            <  2:2735650/150‑4 (MQ=38)
                 tCTATTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGt                           <  2:135780/150‑1 (MQ=38)
                  cTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtc                          <  1:795473/150‑1 (MQ=39)
                   tGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtct                         <  1:4467833/150‑1 (MQ=39)
                    gTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGtctc                        <  1:2579817/150‑1 (MQ=38)
                    gTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGAtctc                        <  1:3595629/150‑1 (MQ=38)
                    gTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGAAGACAAAAAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGAtctc                        <  2:1005070/150‑1 (MQ=38)
                      tgcgaaGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCa                      <  1:143072/146‑1 (MQ=39)
                      tgcgaaGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCa                      <  2:197813/146‑1 (MQ=38)
                       gcgaaGTTTGAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCAt                     <  2:3989452/147‑1 (MQ=38)
                       gcgaaGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCAt                     <  1:2651471/147‑1 (MQ=38)
                       gcgaaGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCAt                     <  2:1664808/147‑1 (MQ=37)
                       gcgaaGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCAt                     <  1:4312919/147‑1 (MQ=39)
                       gcgaaGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCAt                     <  2:3685015/147‑1 (MQ=255)
                       gcgaaGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCAt                     <  1:2050543/147‑1 (MQ=38)
                       gcgaaGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCAt                     <  1:2810588/147‑1 (MQ=38)
                       gcgaaGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCAt                     <  2:1107822/147‑1 (MQ=38)
                        cgaaGTTTGAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATg                    <  2:1427807/148‑1 (MQ=38)
                        cgaaGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATg                    <  1:404446/148‑1 (MQ=255)
                         gaaGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGc                   <  1:3768856/149‑1 (MQ=38)
                          aaGTTTGAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGcc                  <  2:2535564/150‑1 (MQ=38)
                          aaGTTTGAAAATTAAACGCGCACTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGcc                  <  2:2921801/150‑1 (MQ=38)
                          aaGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGcc                  <  1:348038/150‑1 (MQ=38)
                          aaGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGcc                  <  1:939750/150‑1 (MQ=255)
                           aGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGATCTCCATGccc                 <  1:3919827/150‑1 (MQ=38)
                            gTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCt                <  1:2495624/150‑1 (MQ=37)
                            gTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCt                <  1:709434/150‑1 (MQ=255)
                             tttGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCtt               <  1:3704592/150‑1 (MQ=37)
                             tttGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCtt               <  1:1740864/150‑1 (MQ=37)
                             tttGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGAATTGTTTCCATGCCCtt               <  1:2930486/150‑1 (MQ=25)
                             tttGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCtt               <  2:3240640/150‑1 (MQ=255)
                              ttGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTa              <  2:821277/150‑1 (MQ=37)
                              ttGAAAATCAAACGTGCCCTCTCTGAAATCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGACGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTa              <  2:2899417/150‑1 (MQ=37)
                              ttGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGAATTGTTTCCATGCCCTTa              <  1:3639910/150‑1 (MQ=25)
                               tGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTaa             <  1:2761980/150‑1 (MQ=255)
                                gAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAAt            <  1:1829904/150‑1 (MQ=38)
                                gAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTaag            <  2:72361/150‑2 (MQ=37)
                                 aaaaTTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATc           <  2:3520500/150‑1 (MQ=37)
                                 aaaaTCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATc           <  1:1228449/150‑1 (MQ=38)
                                  aaaTTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCg          <  2:3277450/150‑1 (MQ=37)
                                  aaaTCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCg          <  1:874880/150‑1 (MQ=37)
                                  aaaTCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCg          <  2:2037454/150‑1 (MQ=38)
                                   aaTCAAACGTGCCCTCTCTGAACTCCAAATATCTTGGGCTGTGATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCGt         <  1:3229481/150‑1 (MQ=37)
                                   aaTCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGt         <  2:3602239/150‑1 (MQ=255)
                                   aaTCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGATCTCCATGCCCTTAATCGt         <  1:1668271/150‑1 (MQ=38)
                                    aTCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCGTg        <  2:4102747/150‑1 (MQ=37)
                                    aTCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCACTATTACCTCGATTTCAGTAGTCACCGAAAAGCCGAAGAGCGTTCCATTTCTGCCGTTCTTTTTATACATTCCTCGAATGATCTCCATGCCCTTAATCGTg        <  2:4390714/150‑1 (MQ=255)
                                     tCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTgg       <  1:539550/150‑1 (MQ=255)
                                     tCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTgg       <  1:2167754/150‑1 (MQ=255)
                                      cAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGAATTGTTTCCATGCCCTTAATCGTggt      <  1:1338996/150‑2 (MQ=25)
                                      cAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGATCTCCATGCCCTTAATCGTGGa      <  1:2595727/150‑1 (MQ=37)
                                      cAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGa      <  2:442439/150‑1 (MQ=255)
                                      cAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGa      <  1:1091224/150‑1 (MQ=255)
                                       aaaCGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGaa     <  2:3138860/150‑1 (MQ=255)
                                        aaCGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGAATTGTTTCCATGCCCTTAATCGTggttg    <  1:2102873/150‑4 (MQ=25)
                                        aaCGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAag    <  2:2779627/150‑1 (MQ=255)
                                         aCGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGAATTGTTTCCATGCCCTTAATCGTggttga   <  1:3017795/150‑5 (MQ=21)
                                             |                                                                                                                                                  
AAAATAATTTGACTGGTTCTGTTGCAAAGTTTGAAAATCAAACGCACCCGCTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAG  >  CP043523/1861151‑1861342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑