Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,861,200 G→T P118P (CCG→CCT)  FTN78_chr1908 → neutral endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,861,2000GT99.9% 2219.9 / NA 679P118P (CCG→CCT) FTN78_chr1908neutral endopeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (8/670);  total (8/671)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 679 total aligned reads displayed.

AAAATAATTTGACTGGTTCTGTTGCAAAGTTTGAAAATCAAACGCACCCGCTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTG  >  CP043523/1861151‑1861346
                                                 |                                                                                                                                                  
ataaatctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTta                                                <  1:1765175/143‑1 (MQ=255)
ataaatctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTta                                                <  1:3121024/143‑1 (MQ=255)
ataaatctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTta                                                <  1:2281655/143‑1 (MQ=255)
 taaatctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTtat                                               <  2:3364256/144‑1 (MQ=255)
     tctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGATCTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTtatatan                                           <  2:1210574/148‑2 (MQ=255)
     tctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTatatat                                           <  1:322084/148‑1 (MQ=255)
      ctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATAtt                                          <  1:3178468/149‑1 (MQ=255)
       tttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTc                                         <  1:1173263/150‑1 (MQ=255)
       tattggcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTc                                         <  1:4106158/143‑1 (MQ=255)
       aattgatGGTTCTATTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTc                                         <  1:1001819/144‑1 (MQ=38)
        ttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTcc                                        <  2:71458/150‑1 (MQ=255)
        ctatttGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTcc                                        <  1:858512/145‑1 (MQ=255)
        attggcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTcc                                        <  2:95857/144‑1 (MQ=255)
        attggcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTcc                                        <  1:1957509/144‑1 (MQ=255)
         ttgatGGTTCTATTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCt                                       <  2:3424292/146‑1 (MQ=38)
         tatttGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCt                                       <  2:479252/146‑1 (MQ=39)
          tttcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc                                      <  2:1143308/146‑1 (MQ=39)
          tggtGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGATCTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCCGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTc                                      <  1:2612994/147‑1 (MQ=38)
          tggtGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTc                                      <  1:2498049/147‑1 (MQ=38)
          tggcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc                                      <  1:744725/146‑1 (MQ=39)
          tggcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc                                      <  2:4316568/146‑1 (MQ=39)
          taagggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc                                      <  2:1302275/146‑1 (MQ=39)
          aaacggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc                                      <  1:3307463/146‑1 (MQ=255)
           ttcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg                                     <  1:1439257/147‑1 (MQ=39)
           ttcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg                                     <  2:2898065/147‑1 (MQ=39)
           cacgggtCTGTTGCGAAGTTTGAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg                                     <  1:998501/144‑1 (MQ=37)
           aacggTTCTGTTGCGAAGTTTGAATATCAGACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg                                     <  2:4538486/147‑1 (MQ=255)
           aCTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg                                     <  2:2639494/150‑1 (MQ=255)
           aCTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg                                     <  1:1355489/150‑1 (MQ=38)
            atGGTTCTATTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGa                                    <  1:1546900/149‑1 (MQ=38)
            agggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGa                                    <  1:258200/148‑1 (MQ=39)
            acggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGa                                    <  1:2172252/148‑1 (MQ=38)
             tgtttCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaa                                   <  2:2206842/147‑1 (MQ=38)
             tGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaa                                   <  2:2990635/150‑1 (MQ=255)
             cgggtCTGTTGCGAAGTTTGAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaa                                   <  1:2051399/146‑1 (MQ=37)
              ggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAg                                  <  1:3085512/150‑1 (MQ=39)
              ggTTCTATTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAg                                  <  1:2442414/150‑1 (MQ=38)
               tttCTGTTGCGAAGTTTCAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCACTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaatn                                 <  2:3881527/149‑3 (MQ=37)
               gTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAgg                                 <  2:1410955/150‑1 (MQ=39)
                gtCTGTTGCGAAGTTTGAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAggg                                <  2:4011121/149‑1 (MQ=38)
                gtCTGTTGCGAAGTTTGAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAggg                                <  2:4182564/149‑1 (MQ=38)
                 tctattGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGt                               <  1:492814/146‑1 (MQ=38)
                  ctattGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtc                              <  1:4379871/147‑1 (MQ=39)
                  cTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtc                              <  1:1857473/150‑1 (MQ=39)
                  cTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtc                              <  1:786412/150‑1 (MQ=255)
                   tGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGtct                             <  1:2358877/150‑1 (MQ=38)
                   tGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGaattgttt                             <  2:3079743/150‑7 (MQ=38)
                   tGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtct                             <  1:3848425/150‑1 (MQ=38)
                    gTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtctc                            <  1:4546851/150‑1 (MQ=39)
                    gTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtctc                            <  1:2861623/150‑1 (MQ=39)
                    attGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtctc                            <  1:4598870/149‑1 (MQ=39)
                     ttGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTcc                           <  2:579931/150‑1 (MQ=38)
                     ttGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTcc                           <  2:2771632/150‑1 (MQ=38)
                      tgcgaaGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCa                          <  2:197813/146‑1 (MQ=38)
                       gcgaaGTTTGAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCAt                         <  1:2945199/147‑1 (MQ=38)
                       gcgaaGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCAt                         <  2:1664808/147‑1 (MQ=37)
                       gcgaaGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCAt                         <  2:1107822/147‑1 (MQ=38)
                        cgaaGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATg                        <  1:3382217/148‑1 (MQ=38)
                        cgaaGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTCGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATg                        <  1:3579016/148‑1 (MQ=38)
                          aaGTTTGAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGcc                      <  2:2535564/150‑1 (MQ=38)
                          aaGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGcc                      <  2:2920651/150‑1 (MQ=255)
                           aGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGATCTCCATGccc                     <  1:3919827/150‑1 (MQ=38)
                           aGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGccc                     <  1:376582/150‑1 (MQ=38)
                            gTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCt                    <  1:4458318/150‑1 (MQ=255)
                            gTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCt                    <  1:4287751/150‑1 (MQ=255)
                             tttGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCtt                   <  1:2656776/150‑1 (MQ=37)
                             tttGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCtt                   <  1:3053549/150‑1 (MQ=255)
                              ttGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTTCATTTCTGCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTa                  <  2:3725902/150‑1 (MQ=255)
                              ttGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTa                  <  2:821277/150‑1 (MQ=37)
                              ttGAAAATCAAACGCGCCCTCTCTGAACTCGCAATATCTTAGGCGGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTa                  <  2:1847318/150‑1 (MQ=37)
                              ttGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGAATTGTTTCCATGCCCTTa                  <  1:3639910/150‑1 (MQ=25)
                               tGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTan                 <  2:2101834/150‑2 (MQ=255)
                                gAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCGTTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTaag                <  1:3477706/150‑2 (MQ=37)
                                gAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTaag                <  1:2146944/150‑2 (MQ=37)
                                 aaaaTTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATc               <  2:3520500/150‑1 (MQ=37)
                                 aaaaTCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTaagc               <  1:4020054/150‑3 (MQ=37)
                                 aaaaTCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCAGTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTaagc               <  1:1236969/150‑3 (MQ=37)
                                 aaaaTCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATc               <  2:148723/150‑1 (MQ=255)
                                 aaaaTCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATc               <  1:598545/150‑1 (MQ=38)
                                 aaaaTCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATc               <  2:2036143/150‑1 (MQ=38)
                                  aaaTTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCg              <  2:3277450/150‑1 (MQ=37)
                                  aaaTCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCg              <  2:4103709/150‑1 (MQ=37)
                                   aaTCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCGt             <  2:2290006/150‑1 (MQ=37)
                                   aaTCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCGt             <  2:656850/150‑1 (MQ=37)
                                   aaTCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGt             <  2:3602239/150‑1 (MQ=255)
                                   aaTCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGATCTCCATGCCCTTAATCGt             <  1:1674780/150‑1 (MQ=38)
                                    aTCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCACTATTACCTCGATTTCAGTAGTCACCGAAAAGCCGAAGAGCGTTCCATTTCTGCCGTTCTTTTTATACATTCCTCGAATGATCTCCATGCCCTTAATCGTg            <  2:4390714/150‑1 (MQ=255)
                                     tCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTgg           <  1:277961/150‑1 (MQ=255)
                                      cAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGa          <  1:1091224/150‑1 (MQ=255)
                                      cAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGa          <  2:442439/150‑1 (MQ=255)
                                       aaaCGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGaa         <  1:936045/150‑1 (MQ=37)
                                       aaaCGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGaa         <  2:3138860/150‑1 (MQ=255)
                                        aaCGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAag        <  1:2686464/150‑1 (MQ=37)
                                         aCGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAaga       <  1:1589006/150‑1 (MQ=255)
                                         aCGCGCCCTCTCTGAACTCCAAATATCTCAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGAATTGTTTCCATGCCCTTAATCGTggttga       <  1:3018597/150‑5 (MQ=21)
                                          cgtgcccTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCGTGGAagag      <  1:3701071/146‑1 (MQ=37)
                                           gtgcccTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCGTGGAAGAgg     <  1:702143/147‑1 (MQ=37)
                                            cgcccTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGc    <  2:135600/148‑1 (MQ=38)
                                              cccTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCGTGGAAGAggttt  <  1:1993844/150‑4 (MQ=37)
                                              cccGCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCAGTAATACCTTTATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTg  <  2:3224708/150‑1 (MQ=255)
                                                 |                                                                                                                                                  
AAAATAATTTGACTGGTTCTGTTGCAAAGTTTGAAAATCAAACGCACCCGCTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTG  >  CP043523/1861151‑1861346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑