Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,861,227 G→T P225T (CCA→ACA)  FTN78_chr1909 ← Mobile element protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,861,2270GT76.9% 2130.7 / inf 1157P225T (CCA→ACA) FTN78_chr1909Mobile element protein
Reads supporting (aligned to +/- strand):  ref base G (41/225);  new base T (110/777);  total (152/1005)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

Only 100 of 1168 total aligned reads displayed.

AAAATAATTTGACTGGTTCTGTTGCAAAGTTTGAAAATCAAACGCACCCGCTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGATAAAATTTATTCCGTC  >  CP043523/1861151‑1861376
                                                                            |                                                                                                                                                     
ataaatctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTta                                                                              <  1:3121024/143‑1 (MQ=255)
ataaatctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTACCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTta                                                                              <  1:3863467/143‑1 (MQ=255)
   aatctttGTCTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTtatac                                                                           <  2:543977/146‑2 (MQ=255)
     tctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTtatatan                                                                         <  2:1210576/148‑2 (MQ=255)
     tctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTatatat                                                                         <  1:2394263/148‑1 (MQ=255)
      ctttGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATAtt                                                                        <  1:2245647/149‑1 (MQ=38)
       tagaaacggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTc                                                                       <  1:3807128/143‑1 (MQ=255)
       tacaaacggTCCTGTTTCGAAGTTTGAAAATCAAGCGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTACTTCCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTc                                                                       <  1:3707920/143‑1 (MQ=255)
       agatggtGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTc                                                                       <  2:296571/144‑1 (MQ=38)
       aattgatGGTTCTATTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTc                                                                       <  1:1001819/144‑1 (MQ=38)
        attggcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTcc                                                                      <  1:1096653/144‑1 (MQ=255)
         tatttGGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCt                                                                     <  1:2843993/146‑1 (MQ=39)
         tGACTGTTTCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTccn                                                                     <  2:22583/150‑2 (MQ=255)
          aaacggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc                                                                    <  2:4110278/146‑1 (MQ=38)
          aaacggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTc                                                                    <  1:3307461/146‑1 (MQ=255)
           ttcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg                                                                   <  2:2898065/147‑1 (MQ=39)
           ggcggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg                                                                   <  2:1136289/147‑1 (MQ=39)
           cacgggtCTGTTGCGAAGTTTGAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCg                                                                   <  1:998501/144‑1 (MQ=37)
            ctgtttCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGa                                                                  <  1:3920365/146‑1 (MQ=38)
            agggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGa                                                                  <  1:258200/148‑1 (MQ=39)
            acggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGa                                                                  <  1:2172252/148‑1 (MQ=38)
             tgtttCTATTTCTAATTTTTAAAATCAAACTTTCCCTCTCTGAACTCCAAATATCTTATTCTGTTATTCCCATTAATACCTTGATTTCAGTATACATCTAAAATCCGAATATCGTTCCATTTCTTCGTTTCTTTTTATATATTCCTCGaa                                                                 <  2:66198/147‑1 (MQ=255)
             cgggtCTGTTGCGAAGTTTGAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaa                                                                 <  1:2051399/146‑1 (MQ=37)
              ggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAg                                                                <  2:748557/150‑1 (MQ=39)
              ggTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAg                                                                <  2:1764402/150‑1 (MQ=39)
               tttCTGTTGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaatg                                                               <  2:2159584/149‑3 (MQ=38)
               gTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGaatg                                                               <  1:75907/150‑3 (MQ=255)
               gTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAgg                                                               <  2:1410955/150‑1 (MQ=39)
                   tGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGaattgttt                                                           <  2:3079743/150‑7 (MQ=38)
                   tGTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATCTCAGTAGGCATCGAAAAGCCGGAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGtct                                                           <  2:4356165/150‑1 (MQ=38)
                    gTTGCGAAGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGtctc                                                          <  1:4546851/150‑1 (MQ=39)
                    gTTGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGAtctc                                                          <  2:3207573/150‑1 (MQ=38)
                     ttGCGAAGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTcc                                                         <  1:3027562/150‑1 (MQ=38)
                     ttGCGAAGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGATCTcc                                                         <  1:682507/150‑1 (MQ=38)
                      tgcgaaGTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCa                                                        <  2:1562393/146‑1 (MQ=39)
                      tgcgaaGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCa                                                        <  2:2569067/146‑1 (MQ=38)
                      tgcgaaGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCa                                                        <  2:197813/146‑1 (MQ=38)
                       gcgaaGTTTGAAAATTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCAt                                                       <  2:3989452/147‑1 (MQ=38)
                         gaaGTTTGAAACTCAAACGTGCCCTCTCTGAACTCCAAATTTCTTAGGCTGTTATTCCCCTTAATACCTTGATTTCCGTAGGCATCGAAATGCCGACGAGCGTGCTATTTCTTCGGTTCTTTTTATATATTCCTCGAATAGTCTCCATGc                                                     <  2:2749621/149‑1 (MQ=25)
                          aaGTTTGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGcc                                                    <  1:340219/150‑1 (MQ=38)
                          aaGTTTGAAAATCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGAATTGTTTCCATGcc                                                    <  1:3530585/150‑1 (MQ=37)
                            gTTTGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCt                                                  <  1:4287751/150‑1 (MQ=255)
                             tttGAAAATCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCtt                                                 <  2:337930/150‑1 (MQ=38)
                              ttGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTa                                                <  1:3093666/150‑1 (MQ=255)
                               tGAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTaa                                               <  1:248658/150‑1 (MQ=255)
                                gAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAAt                                              <  1:955421/150‑1 (MQ=255)
                                gAAAATCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAAt                                              <  2:4214367/150‑1 (MQ=255)
                                  aaaTTAAACGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCg                                            <  2:3275793/150‑1 (MQ=38)
                                  aaaTCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGATCTCCATGCCCTTAATCg                                            <  2:3789324/150‑1 (MQ=38)
                                   aaTCAAACGTGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCGt                                           <  1:2124760/150‑1 (MQ=37)
                                   aaTCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGt                                           <  2:3603461/150‑1 (MQ=255)
                                    aTCAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGAATTGTTTCCATGCCCTTAATCGTg                                          <  2:4575184/150‑1 (MQ=25)
                                     tCAAACGCGCCCTTTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTgg                                         <  1:277961/150‑1 (MQ=255)
                                      cAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGAATTGTTTCCATGCCCTTAATCGTggt                                        <  1:1338996/150‑2 (MQ=25)
                                      cAAACGCGCCCTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGa                                        <  2:442439/150‑1 (MQ=255)
                                        aaCGCGCCCTCTCTGAACTCCGAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAag                                      <  1:2687184/150‑1 (MQ=37)
                                          cgcgCCCTCTCTTAACTCCAAATATCTTAGGCTGTTATTCCCATTAATCCCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAagag                                    <  1:1288335/150‑1 (MQ=37)
                                            tggcctctctGAACTCCAAATATCTTAGGTGGTTATTCCCATTGATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCGTGGAAGAggt                                  <  1:4004044/144‑2 (MQ=25)
                                            tgccctctctGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCGTGGAAGAggt                                  <  1:1516454/144‑2 (MQ=37)
                                             gccctctctGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCGTGGAAGAggtt                                 <  2:929248/145‑3 (MQ=37)
                                             gccctctctGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCGTGGAAGAggtt                                 <  1:3223473/145‑3 (MQ=37)
                                              cccTCTCTGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCGTGGAAGAggttt                                <  1:3170363/150‑4 (MQ=37)
                                               cctctctGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCGTGGAAGAggtttg                               <  2:204145/147‑5 (MQ=37)
                                               cctctctGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCGTGGAAGAggtttg                               <  1:4068975/147‑5 (MQ=37)
                                                 tctctGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACATCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGGTCTCCATGCCCTTAAGCGTGGAAGAggtttgat                             <  1:163941/149‑7 (MQ=37)
                                                   tctGAACTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGATCTCCATGCCCTTAATCGTGGAAAAGGCTGTACgg                           <  1:554578/150‑1 (MQ=38)
                                                     tGAACTCCAAATATCTTAGGCTGTTATTCCCCTTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGATCTCCATGCCCTTAATCGTGGAAAAGGCTGTACGgag                         <  1:4142037/150‑1 (MQ=38)
                                                       aaCTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGAc                       <  1:2619649/150‑1 (MQ=255)
                                                       aaCTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGAc                       >  1:2909696/1‑150 (MQ=255)
                                                        aCTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGATCTCCATGCCCTTAATCGTGGAAAAGGCTGTACGGAGACt                      >  1:610830/1‑150 (MQ=38)
                                                        aCTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGATCTCCATGCCCTTAATCGTGGAAAAGGCTGTACGGAGACt                      >  1:3589674/1‑150 (MQ=38)
                                                        aCTCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACt                      >  1:177971/1‑150 (MQ=255)
                                                        aCTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACt                      <  1:2081468/150‑1 (MQ=255)
                                                         cTCCAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGAATTGTTTCCATGCCCTTAATCGTGGTTGAGGCAGTTCGTAGACtt                     >  1:2319273/1‑150 (MQ=18)
                                                          tCCAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACAGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACttt                    <  1:3461462/150‑1 (MQ=255)
                                                          tCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACttt                    >  1:887099/1‑150 (MQ=255)
                                                          tCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACttt                    >  1:1670561/1‑150 (MQ=255)
                                                          tCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACttt                    <  1:4484523/150‑1 (MQ=255)
                                                           ccAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGAATTGTTTCCATGCCCTTAATCGTGGTTGAGGCAGTTCGTAGACttcg                   >  1:419649/1‑148 (MQ=17)
                                                           ccAAATATCTTAGGTTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTGTATATTCCTCGAATTGTTTCCATGCCCTTAATCGTGGTTGAGGCAGTTCGTAGACttcg                   >  1:2518779/1‑148 (MQ=17)
                                                           ccAAATATCTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTg                   >  1:3060550/1‑150 (MQ=255)
                                                           ccAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTg                   >  1:1725136/1‑150 (MQ=255)
                                                            cAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGa                  <  1:1439850/150‑1 (MQ=255)
                                                             aaaTATCCTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGAt                 <  1:1851371/150‑1 (MQ=255)
                                                              aaTATCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGATa                >  1:2958752/1‑150 (MQ=255)
                                                                tatCTTAGGCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGATaaa              <  1:500104/150‑1 (MQ=255)
                                                                   cTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAATGATCTCCATGCCCTTAATCGTGGAAAAGGCTGTACGGAGACTTTGATAAAAtt           >  1:823842/1‑150 (MQ=37)
                                                                   cTTAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGATAAAAtt           <  1:4353997/150‑1 (MQ=255)
                                                                   cTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGATAAAAtt           >  1:1960822/1‑150 (MQ=255)
                                                                    ttAGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGATAAAAttt          <  1:3646746/150‑1 (MQ=255)
                                                                    ttAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGATAAAAttt          <  1:1566641/150‑1 (MQ=255)
                                                                     gaGGCTGTTATTCCCATTAATACCTTGATGTAAGTAGACACTGAAAAGCCGAAGAGCGCTCCATTTCTTCGGGCCTTTTCAGAGATTCCTCGAAGGGTCTCCATGCCCTTAATCGTAGAAGAGGCTGTACGCAGACTTTGATAAAATTTa         >  2:3520350/2‑150 (MQ=255)
                                                                      aGGCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGATAAAATTTAt        >  1:4428453/1‑150 (MQ=255)
                                                                       ggCTGTTATTCCCATTAATACCTTGATTTAAGTAGACACTGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGATAAAATTTAtt       >  1:3345324/1‑150 (MQ=255)
                                                                       ggCTGGTATTCCCATTAATACCTTCATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGATAAAATTTAtt       <  2:1346419/150‑1 (MQ=255)
                                                                        gCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGATAAAATTTAttt      <  1:2664008/150‑2 (MQ=255)
                                                                        gCTGTTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGATAAAATTTAttt      >  1:118728/1‑149 (MQ=255)
                                                                          tGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGATAAAATTTATTCcg    >  1:1986899/1‑150 (MQ=255)
                                                                          tGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGATAAAATTTATTCcg    <  1:4322154/150‑1 (MQ=255)
                                                                           ggTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGATAAAATTTATTCcgt   <  1:4600277/150‑1 (MQ=255)
                                                                            |                                                                                                                                                     
AAAATAATTTGACTGGTTCTGTTGCAAAGTTTGAAAATCAAACGCACCCGCTCTGAACTCCAAATATCTTAGGCTGGTATTCCCATTAATACCTTGATTTCAGTAGACACCGAAAAGCCGAAGAGCGTTCCATTTCTTCGGTTCTTTTTATATATTCCTCGAAGGGTCTCCATGCCCTTAATCGTGGAAGAGGCTGTACGGAGACTTTGATAAAATTTATTCCGTC  >  CP043523/1861151‑1861376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑